Análise genotípica e filogenética com base nos genes das glicoproteínas C e D de herpesvírus bovino 1 e 5
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/4095 |
Resumo: | Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) are genetic and antigenic closely related pathogens of cattle, historically associated with respiratory/genital and neurological disease, respectively. This thesis reports the genomic and phylogenetic analyzes based on glycoproteins C (gC) and D (gD) genes of BoHV-1 and BoHV-5. Initially, the differentiation between BoHV-1 and BoHV-5 was performed by a differential PCR based on 5 gC gene. Then, a molecular analysis based on the 3 region of the gC gene of 45 BoHV isolates from Brazil, Uruguay and Argentina (1981-2009) was described. The phylogenetic tree reconstruction provided a clear distinction between BoHV-1 and BoHV-5, and BoHV-1 into subtypes BoHV-1.1 and BoHV-1.2. The levels of nucleotide (nt) similarity ranged from 99.1 to 100% among BoHV-1 sequences (n=12); 96.2-100% among BoHV-5 sequences (n=32); and 77.7-90.3% between BoHV-1 and BoHV-5 sequences. A transmembrane domain of 24 amino-acid (aa) and the putative cytoplasmic tail of 8 aa were also identified. In addition, a phylogenetic study was performed to investigate genetic divergences at the 3 region of gD gene of respiratory/genital BoHV-1 (n=7), neurological BoHV-1 (n=7) and neurological BoHV-5 (n=7) isolates, and whether these differences would be associated with the respective neurological presentation. The phylogenetic reconstruction allowed a clear differentiation of BoHV-1 (n=14) and BoHV-5 (n=7), but BoHV-1 isolates from neurological disease grouped within BoHV-1 branch. The nt and aa similarity levels were on average 98.3% among BoHV-1; 97.8% and 95.8% among BoHV-5; 73.7% and 64.1% between viral species. These results indicate that both genes revealed a high conserved 3 region within each species and a less conserved region between BoHV-1 and BoHV-5. The phylogenetic analyzes allowed differentiation of BoHV-1 and BoHV-5 species, and even subtypes grouped in distinct branches in the 3 gC gene-based study, indicating that this region represents a better choice for phylogenetic subgrouping. So, it was concluded that these genome regions represent a suitable target for phylogenetic classification of BoHV-1 and BoHV-5 isolates, and, perhaps, for understanding evolutionary relationships. However, no conclusion of a possible association of genetic differences with phenotypes could be drawn. |
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Análise genotípica e filogenética com base nos genes das glicoproteínas C e D de herpesvírus bovino 1 e 5Genotypic analysis and phylogeny based on glycoproteins C and D genes of bovine herpesvirusesBoHV-1BoHV-5GCGDFilogeniaPhylogenyCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIABovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) are genetic and antigenic closely related pathogens of cattle, historically associated with respiratory/genital and neurological disease, respectively. This thesis reports the genomic and phylogenetic analyzes based on glycoproteins C (gC) and D (gD) genes of BoHV-1 and BoHV-5. Initially, the differentiation between BoHV-1 and BoHV-5 was performed by a differential PCR based on 5 gC gene. Then, a molecular analysis based on the 3 region of the gC gene of 45 BoHV isolates from Brazil, Uruguay and Argentina (1981-2009) was described. The phylogenetic tree reconstruction provided a clear distinction between BoHV-1 and BoHV-5, and BoHV-1 into subtypes BoHV-1.1 and BoHV-1.2. The levels of nucleotide (nt) similarity ranged from 99.1 to 100% among BoHV-1 sequences (n=12); 96.2-100% among BoHV-5 sequences (n=32); and 77.7-90.3% between BoHV-1 and BoHV-5 sequences. A transmembrane domain of 24 amino-acid (aa) and the putative cytoplasmic tail of 8 aa were also identified. In addition, a phylogenetic study was performed to investigate genetic divergences at the 3 region of gD gene of respiratory/genital BoHV-1 (n=7), neurological BoHV-1 (n=7) and neurological BoHV-5 (n=7) isolates, and whether these differences would be associated with the respective neurological presentation. The phylogenetic reconstruction allowed a clear differentiation of BoHV-1 (n=14) and BoHV-5 (n=7), but BoHV-1 isolates from neurological disease grouped within BoHV-1 branch. The nt and aa similarity levels were on average 98.3% among BoHV-1; 97.8% and 95.8% among BoHV-5; 73.7% and 64.1% between viral species. These results indicate that both genes revealed a high conserved 3 region within each species and a less conserved region between BoHV-1 and BoHV-5. The phylogenetic analyzes allowed differentiation of BoHV-1 and BoHV-5 species, and even subtypes grouped in distinct branches in the 3 gC gene-based study, indicating that this region represents a better choice for phylogenetic subgrouping. So, it was concluded that these genome regions represent a suitable target for phylogenetic classification of BoHV-1 and BoHV-5 isolates, and, perhaps, for understanding evolutionary relationships. However, no conclusion of a possible association of genetic differences with phenotypes could be drawn.Conselho Nacional de Desenvolvimento Científico e TecnológicoBovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) are genetic and antigenic closely related pathogens of cattle, historically associated with respiratory/genital and neurological disease, respectively. This thesis reports the genomic and phylogenetic analyzes based on glycoproteins C (gC) and D (gD) genes of BoHV-1 and BoHV-5. Initially, the differentiation between BoHV-1 and BoHV-5 was performed by a differential PCR based on 5 gC gene. Then, a molecular analysis based on the 3 region of the gC gene of 45 BoHV isolates from Brazil, Uruguay and Argentina (1981-2009) was described. The phylogenetic tree reconstruction provided a clear distinction between BoHV-1 and BoHV-5, and BoHV-1 into subtypes BoHV-1.1 and BoHV-1.2. The levels of nucleotide (nt) similarity ranged from 99.1 to 100% among BoHV-1 sequences (n=12); 96.2-100% among BoHV-5 sequences (n=32); and 77.7-90.3% between BoHV-1 and BoHV-5 sequences. A transmembrane domain of 24 amino-acid (aa) and the putative cytoplasmic tail of 8 aa were also identified. In addition, a phylogenetic study was performed to investigate genetic divergences at the 3 region of gD gene of respiratory/genital BoHV-1 (n=7), neurological BoHV-1 (n=7) and neurological BoHV-5 (n=7) isolates, and whether these differences would be associated with the respective neurological presentation. The phylogenetic reconstruction allowed a clear differentiation of BoHV-1 (n=14) and BoHV-5 (n=7), but BoHV-1 isolates from neurological disease grouped within BoHV-1 branch. The nt and aa similarity levels were on average 98.3% among BoHV-1; 97.8% and 95.8% among BoHV-5; 73.7% and 64.1% between viral species. These results indicate that both genes revealed a high conserved 3 region within each species and a less conserved region between BoHV-1 and BoHV-5. The phylogenetic analyzes allowed differentiation of BoHV-1 and BoHV-5 species, and even subtypes grouped in distinct branches in the 3 gC gene-based study, indicating that this region represents a better choice for phylogenetic subgrouping. So, it was concluded that these genome regions represent a suitable target for phylogenetic classification of BoHV-1 and BoHV-5 isolates, and, perhaps, for understanding evolutionary relationships. However, no conclusion of a possible association of genetic differences with phenotypes could be drawn.Universidade Federal de Santa MariaBRMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaFlores, Eduardo Furtadohttp://lattes.cnpq.br/0446078331070694Weiblen, Rudihttp://lattes.cnpq.br/7946350215388090Kreutz, Luiz Carloshttp://lattes.cnpq.br/6207090944092820Spilki, Fernando Rosadohttp://lattes.cnpq.br/3481593940960227Brum, Mário Celso Sperottohttp://lattes.cnpq.br/9761857774819478Traesel, Carolina Kist2017-05-302017-05-302013-03-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfTRAESEL, Carolina Kist. Genotypic analysis and phylogeny based on glycoproteins C and D genes of bovine herpesviruses. 2013. 80 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2013.http://repositorio.ufsm.br/handle/1/4095porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-04-08T19:12:12Zoai:repositorio.ufsm.br:1/4095Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-04-08T19:12:12Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Análise genotípica e filogenética com base nos genes das glicoproteínas C e D de herpesvírus bovino 1 e 5 Genotypic analysis and phylogeny based on glycoproteins C and D genes of bovine herpesviruses |
title |
Análise genotípica e filogenética com base nos genes das glicoproteínas C e D de herpesvírus bovino 1 e 5 |
spellingShingle |
Análise genotípica e filogenética com base nos genes das glicoproteínas C e D de herpesvírus bovino 1 e 5 Traesel, Carolina Kist BoHV-1 BoHV-5 GC GD Filogenia Phylogeny CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Análise genotípica e filogenética com base nos genes das glicoproteínas C e D de herpesvírus bovino 1 e 5 |
title_full |
Análise genotípica e filogenética com base nos genes das glicoproteínas C e D de herpesvírus bovino 1 e 5 |
title_fullStr |
Análise genotípica e filogenética com base nos genes das glicoproteínas C e D de herpesvírus bovino 1 e 5 |
title_full_unstemmed |
Análise genotípica e filogenética com base nos genes das glicoproteínas C e D de herpesvírus bovino 1 e 5 |
title_sort |
Análise genotípica e filogenética com base nos genes das glicoproteínas C e D de herpesvírus bovino 1 e 5 |
author |
Traesel, Carolina Kist |
author_facet |
Traesel, Carolina Kist |
author_role |
author |
dc.contributor.none.fl_str_mv |
Flores, Eduardo Furtado http://lattes.cnpq.br/0446078331070694 Weiblen, Rudi http://lattes.cnpq.br/7946350215388090 Kreutz, Luiz Carlos http://lattes.cnpq.br/6207090944092820 Spilki, Fernando Rosado http://lattes.cnpq.br/3481593940960227 Brum, Mário Celso Sperotto http://lattes.cnpq.br/9761857774819478 |
dc.contributor.author.fl_str_mv |
Traesel, Carolina Kist |
dc.subject.por.fl_str_mv |
BoHV-1 BoHV-5 GC GD Filogenia Phylogeny CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
BoHV-1 BoHV-5 GC GD Filogenia Phylogeny CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) are genetic and antigenic closely related pathogens of cattle, historically associated with respiratory/genital and neurological disease, respectively. This thesis reports the genomic and phylogenetic analyzes based on glycoproteins C (gC) and D (gD) genes of BoHV-1 and BoHV-5. Initially, the differentiation between BoHV-1 and BoHV-5 was performed by a differential PCR based on 5 gC gene. Then, a molecular analysis based on the 3 region of the gC gene of 45 BoHV isolates from Brazil, Uruguay and Argentina (1981-2009) was described. The phylogenetic tree reconstruction provided a clear distinction between BoHV-1 and BoHV-5, and BoHV-1 into subtypes BoHV-1.1 and BoHV-1.2. The levels of nucleotide (nt) similarity ranged from 99.1 to 100% among BoHV-1 sequences (n=12); 96.2-100% among BoHV-5 sequences (n=32); and 77.7-90.3% between BoHV-1 and BoHV-5 sequences. A transmembrane domain of 24 amino-acid (aa) and the putative cytoplasmic tail of 8 aa were also identified. In addition, a phylogenetic study was performed to investigate genetic divergences at the 3 region of gD gene of respiratory/genital BoHV-1 (n=7), neurological BoHV-1 (n=7) and neurological BoHV-5 (n=7) isolates, and whether these differences would be associated with the respective neurological presentation. The phylogenetic reconstruction allowed a clear differentiation of BoHV-1 (n=14) and BoHV-5 (n=7), but BoHV-1 isolates from neurological disease grouped within BoHV-1 branch. The nt and aa similarity levels were on average 98.3% among BoHV-1; 97.8% and 95.8% among BoHV-5; 73.7% and 64.1% between viral species. These results indicate that both genes revealed a high conserved 3 region within each species and a less conserved region between BoHV-1 and BoHV-5. The phylogenetic analyzes allowed differentiation of BoHV-1 and BoHV-5 species, and even subtypes grouped in distinct branches in the 3 gC gene-based study, indicating that this region represents a better choice for phylogenetic subgrouping. So, it was concluded that these genome regions represent a suitable target for phylogenetic classification of BoHV-1 and BoHV-5 isolates, and, perhaps, for understanding evolutionary relationships. However, no conclusion of a possible association of genetic differences with phenotypes could be drawn. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-08 2017-05-30 2017-05-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
TRAESEL, Carolina Kist. Genotypic analysis and phylogeny based on glycoproteins C and D genes of bovine herpesviruses. 2013. 80 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2013. http://repositorio.ufsm.br/handle/1/4095 |
identifier_str_mv |
TRAESEL, Carolina Kist. Genotypic analysis and phylogeny based on glycoproteins C and D genes of bovine herpesviruses. 2013. 80 f. Tese (Doutorado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2013. |
url |
http://repositorio.ufsm.br/handle/1/4095 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922171470479360 |