Caracterização dos efeitos letais e subletais da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para espécies de Spodoptera (Lepidoptera: Noctuidae)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/27031 |
Resumo: | MON 87701 × MON 89788 × MON 87751 × MON 87708 soybean that expresses Cry1A.105, Cry2Ab2, and Cry1Ac insecticidal proteins and confers tolerance to glyphosate and dicamba is a potential tool for managing Spodoptera species in soybean in Brazil. In this study, we characterized the lethal and sub-lethal effects of Cry1A.105/Cry2Ab2/Cry1Ac soybean against Spodoptera cosmioides (Walker, 1898), Spodoptera eridania (Stoll 1782), Spodoptera albula (Walker, 1857) and genotypes of Spodoptera frugiperda (J. E. Smith 1797) (Lepidoptera: Noctuidae) resistant and susceptible to Cry1 and Cry2 proteins. These evaluations were also conducted with MON 87701 × MON 89788 soybean, which expresses Cry1Ac protein. Initially, bioassays were performed with Bt and non-Bt soybean leaves removed from plants at the phenological stages V4-5, V7-8, R2-3 and R3-4 to evaluate the neonate survivorship. Subsequently, the development of the species was also evaluated throughout the biological cycle in Bt and non-Bt soybeans. Finally, the fertility life table of Spodoptera species and was estimated to identify effects on population growth parameters. Results showed that Cry1A.105/Cry2Ab2/Cry1Ac soybean had high lethality (>92%) against neonates of S. cosmioides and S. albula. However, it showed low lethality in S. frugiperda genotypes homozygous for resistance to Cry1 and Cry2 proteins but reduced their population growth potential. Spodoptera frugiperda genotypes susceptible and heterozygous for Cry1 and Cry2 resistance were controlled by Cry1A.105/Cry2Ab2/Cry1Ac soybean, with no insects completing the biological cycle. This Bt soybean also caused intermediate mortality (60–83%) of neonates of S. eridania but no surviving larvae developed to adulthood, resulting in population suppression. No relevant lethal and sublethal effects of Cry1Ac soybean were detected in the Spodoptera species and genotypes evaluated. In summary, Cry1A.105/Cry2Ab2/Cry1Ac soybean caused high mortality of S. cosmioides, S. albula, and S. frugiperda genotypes susceptible to Cry1 and Cry2 and heterozygous for Cry1 and Cry2 resistance. This soybean also suppressed population growth of S. eridania but had minimal effects on S. frugiperda homozygous for resistance to Cry1 and Cry2 proteins. Cry1Ac soybean had minimal effects on all Spodoptera species and genotypes evaluated. |
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Caracterização dos efeitos letais e subletais da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para espécies de Spodoptera (Lepidoptera: Noctuidae)Characterizing the letal and sublethal effects of soybean expressing Cry1A.105, Cry2Ab2 and Cry1Ac insecticidal proteins for Spodoptera species (Lepidoptera: Noctuidae)Soja BtLagartas desfolhadorasTabela de vida de fertilidadeManejo integrado de pragasBt soybeanDefoliating speciesLife history traitsIntegrated pest managementCNPQ::CIENCIAS AGRARIAS::AGRONOMIAMON 87701 × MON 89788 × MON 87751 × MON 87708 soybean that expresses Cry1A.105, Cry2Ab2, and Cry1Ac insecticidal proteins and confers tolerance to glyphosate and dicamba is a potential tool for managing Spodoptera species in soybean in Brazil. In this study, we characterized the lethal and sub-lethal effects of Cry1A.105/Cry2Ab2/Cry1Ac soybean against Spodoptera cosmioides (Walker, 1898), Spodoptera eridania (Stoll 1782), Spodoptera albula (Walker, 1857) and genotypes of Spodoptera frugiperda (J. E. Smith 1797) (Lepidoptera: Noctuidae) resistant and susceptible to Cry1 and Cry2 proteins. These evaluations were also conducted with MON 87701 × MON 89788 soybean, which expresses Cry1Ac protein. Initially, bioassays were performed with Bt and non-Bt soybean leaves removed from plants at the phenological stages V4-5, V7-8, R2-3 and R3-4 to evaluate the neonate survivorship. Subsequently, the development of the species was also evaluated throughout the biological cycle in Bt and non-Bt soybeans. Finally, the fertility life table of Spodoptera species and was estimated to identify effects on population growth parameters. Results showed that Cry1A.105/Cry2Ab2/Cry1Ac soybean had high lethality (>92%) against neonates of S. cosmioides and S. albula. However, it showed low lethality in S. frugiperda genotypes homozygous for resistance to Cry1 and Cry2 proteins but reduced their population growth potential. Spodoptera frugiperda genotypes susceptible and heterozygous for Cry1 and Cry2 resistance were controlled by Cry1A.105/Cry2Ab2/Cry1Ac soybean, with no insects completing the biological cycle. This Bt soybean also caused intermediate mortality (60–83%) of neonates of S. eridania but no surviving larvae developed to adulthood, resulting in population suppression. No relevant lethal and sublethal effects of Cry1Ac soybean were detected in the Spodoptera species and genotypes evaluated. In summary, Cry1A.105/Cry2Ab2/Cry1Ac soybean caused high mortality of S. cosmioides, S. albula, and S. frugiperda genotypes susceptible to Cry1 and Cry2 and heterozygous for Cry1 and Cry2 resistance. This soybean also suppressed population growth of S. eridania but had minimal effects on S. frugiperda homozygous for resistance to Cry1 and Cry2 proteins. Cry1Ac soybean had minimal effects on all Spodoptera species and genotypes evaluated.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESA soja MON 87701 × MON 89788 × MON 87751 × MON 87708 que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac de Bacillus thuringiensis (Berliner) (Bt) e confere tolerância aos herbicidas glifosato e dicamba pode ser uma opção para o manejo de espécies do gênero Spodoptera em soja no Brasil. Neste estudo, caracterizou-se os efeitos letais e subletais da soja com expressão de Cry1A.105/Cry2Ab2/Cry1Ac e soja expressando Cry1Ac para Spodoptera cosmioides (Walker, 1898), Spodoptera eridania (Stoll, 1782), Spodoptera albula (Walker, 1857) e genótipos de Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) que são resistentes e suscetíveis às proteínas inseticidas Cry1 e Cry2. Inicialmente, foram realizados bioensaios com folhas de soja Bt e não-Bt nos estádios fenológicos V4-5, V7-8, R2-3 e R3-4 para avaliar a mortalidade de neonatas. Posteriormente, avaliou-se o desenvolvimento das espécies de Spodoptera em soja Bt e não-Bt. Por fim, estimou-se a tabela de vida de fertilidade em soja Bt e não-Bt para quantificar os efeitos em parâmetros de crescimento populacional. Os resultados demostraram que a tecnologia de soja que expressa Cry1A.105/Cry2Ab2/Cry1Ac causou alta mortalidade (>92%) de S. cosmioides e S. albula. Entretanto, apresentou baixa letalidade para linhagens de S. frugiperda resistentes a proteínas Cry1 e Cry2, contudo reduziu o potencial de crescimento populacional. Os genótipos de S. frugiperda sucetível e heterozigotos para resistência a Cry1 e Cry2 apresentaram 100% de mortalidade em soja expressando Cry1A.105/Cry2Ab2/Cry1Ac. Em contraste, esta soja Bt causou mortalidade intermediária (60–83%) de neonatas de S. eridania, mas nenhuma larva completou o ciclo biológico, resultando em supressão populacional. Não foram detectados efeitos letais significativos da soja com expressão de Cry1Ac nas espécies de Spodoptera e linhagens de S. frugiperda. Conclui-se que a soja com expressão de Cry1A.105/Cry2Ab2/Cry1Ac causa elevada mortalidade de S. cosmioides, S. albula e genótipos de S. frugiperda suscetível a Cry1 e Cry2 e heterozigotos para resistência a Cry1 e Cry2. Esta soja Bt também suprimiu o crescimento populacional de S. eridania, mas apresentou baixa atividade biológica para S. frugiperda com resistência a Cry1 e Cry2. A soja com expressão de Cry1Ac teve efeitos letais e subletais mínimos em todas as espécies e genótipos de Spodoptera avaliados.Universidade Federal de Santa MariaBrasilAgronomiaUFSMPrograma de Pós-Graduação em AgronomiaCentro de Ciências RuraisBernardi, Oderleihttp://lattes.cnpq.br/3085175517473196Melo, Adriano ArruéFranco, Claudio RobertoBarcellos, Giovani Antonello2022-11-21T19:46:20Z2022-11-21T19:46:20Z2022-10-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/27031porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-11-21T19:46:20Zoai:repositorio.ufsm.br:1/27031Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-11-21T19:46:20Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Caracterização dos efeitos letais e subletais da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para espécies de Spodoptera (Lepidoptera: Noctuidae) Characterizing the letal and sublethal effects of soybean expressing Cry1A.105, Cry2Ab2 and Cry1Ac insecticidal proteins for Spodoptera species (Lepidoptera: Noctuidae) |
title |
Caracterização dos efeitos letais e subletais da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para espécies de Spodoptera (Lepidoptera: Noctuidae) |
spellingShingle |
Caracterização dos efeitos letais e subletais da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para espécies de Spodoptera (Lepidoptera: Noctuidae) Barcellos, Giovani Antonello Soja Bt Lagartas desfolhadoras Tabela de vida de fertilidade Manejo integrado de pragas Bt soybean Defoliating species Life history traits Integrated pest management CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Caracterização dos efeitos letais e subletais da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para espécies de Spodoptera (Lepidoptera: Noctuidae) |
title_full |
Caracterização dos efeitos letais e subletais da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para espécies de Spodoptera (Lepidoptera: Noctuidae) |
title_fullStr |
Caracterização dos efeitos letais e subletais da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para espécies de Spodoptera (Lepidoptera: Noctuidae) |
title_full_unstemmed |
Caracterização dos efeitos letais e subletais da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para espécies de Spodoptera (Lepidoptera: Noctuidae) |
title_sort |
Caracterização dos efeitos letais e subletais da soja que expressa as proteínas inseticidas Cry1A.105, Cry2Ab2 e Cry1Ac para espécies de Spodoptera (Lepidoptera: Noctuidae) |
author |
Barcellos, Giovani Antonello |
author_facet |
Barcellos, Giovani Antonello |
author_role |
author |
dc.contributor.none.fl_str_mv |
Bernardi, Oderlei http://lattes.cnpq.br/3085175517473196 Melo, Adriano Arrué Franco, Claudio Roberto |
dc.contributor.author.fl_str_mv |
Barcellos, Giovani Antonello |
dc.subject.por.fl_str_mv |
Soja Bt Lagartas desfolhadoras Tabela de vida de fertilidade Manejo integrado de pragas Bt soybean Defoliating species Life history traits Integrated pest management CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
topic |
Soja Bt Lagartas desfolhadoras Tabela de vida de fertilidade Manejo integrado de pragas Bt soybean Defoliating species Life history traits Integrated pest management CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
MON 87701 × MON 89788 × MON 87751 × MON 87708 soybean that expresses Cry1A.105, Cry2Ab2, and Cry1Ac insecticidal proteins and confers tolerance to glyphosate and dicamba is a potential tool for managing Spodoptera species in soybean in Brazil. In this study, we characterized the lethal and sub-lethal effects of Cry1A.105/Cry2Ab2/Cry1Ac soybean against Spodoptera cosmioides (Walker, 1898), Spodoptera eridania (Stoll 1782), Spodoptera albula (Walker, 1857) and genotypes of Spodoptera frugiperda (J. E. Smith 1797) (Lepidoptera: Noctuidae) resistant and susceptible to Cry1 and Cry2 proteins. These evaluations were also conducted with MON 87701 × MON 89788 soybean, which expresses Cry1Ac protein. Initially, bioassays were performed with Bt and non-Bt soybean leaves removed from plants at the phenological stages V4-5, V7-8, R2-3 and R3-4 to evaluate the neonate survivorship. Subsequently, the development of the species was also evaluated throughout the biological cycle in Bt and non-Bt soybeans. Finally, the fertility life table of Spodoptera species and was estimated to identify effects on population growth parameters. Results showed that Cry1A.105/Cry2Ab2/Cry1Ac soybean had high lethality (>92%) against neonates of S. cosmioides and S. albula. However, it showed low lethality in S. frugiperda genotypes homozygous for resistance to Cry1 and Cry2 proteins but reduced their population growth potential. Spodoptera frugiperda genotypes susceptible and heterozygous for Cry1 and Cry2 resistance were controlled by Cry1A.105/Cry2Ab2/Cry1Ac soybean, with no insects completing the biological cycle. This Bt soybean also caused intermediate mortality (60–83%) of neonates of S. eridania but no surviving larvae developed to adulthood, resulting in population suppression. No relevant lethal and sublethal effects of Cry1Ac soybean were detected in the Spodoptera species and genotypes evaluated. In summary, Cry1A.105/Cry2Ab2/Cry1Ac soybean caused high mortality of S. cosmioides, S. albula, and S. frugiperda genotypes susceptible to Cry1 and Cry2 and heterozygous for Cry1 and Cry2 resistance. This soybean also suppressed population growth of S. eridania but had minimal effects on S. frugiperda homozygous for resistance to Cry1 and Cry2 proteins. Cry1Ac soybean had minimal effects on all Spodoptera species and genotypes evaluated. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-21T19:46:20Z 2022-11-21T19:46:20Z 2022-10-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/27031 |
url |
http://repositorio.ufsm.br/handle/1/27031 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Agronomia UFSM Programa de Pós-Graduação em Agronomia Centro de Ciências Rurais |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Agronomia UFSM Programa de Pós-Graduação em Agronomia Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922118658949120 |