DNA barcode de drosofilídeos micófagos pertencentes aos gêneros Hirtodrosophila, Mycodrosophila e Zygothrica
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações do UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/5281 |
Resumo: | The biodiversity that exists in our planet is huge and far from being known. Most of the times the techniques that are used in species identification are based in the morphology of the specimens, and the description is a time-consuming work, that is limited to specialists. At contrast, DNA Barcode is intended to be a fast and accessible tool, which proposes the use of a standard DNA sequence encompassing the mitochondrial cytochrome c oxidase subunit I (COI) gene, with the aim of identifying species from already described sequences and discovering new species. Nevertheless, its efficiency is based on the diagnosis of specific properties, as monophyly of the intraspecific sequences and the existence of a Barcode gap between intra and interspecific variation. In this study, we tested the efficacy of the DNA Barcode in the identification/discovery of mycophagous drosofilid species belonging to the genera Hirtodrosophila, Mycodrosophila and Zygothrica. The specimens were collected throughout the southern Brazil, specially in the Santa Catarina and Rio Grande do Sul states, which totalized 10 collection points and 177 individuals. After morphological identification, total DNA was extracted and the COI (cytochrome oxidase c subunit I) and COII (cytochrome oxidase c subunit II genes) were amplified and sequenced, holding a total of 117 and 137 sequences, respectively, which were analyzed through phenetic and phylogenetic methods. A total of 33 different morphotypes were encountered and, with the collections it was possible to realize that the mycophagous genera Hirtodrosophila, Mycodrosophila and Zygothrica are well distributed throughout the Southern Brazilian Region. Furthermore, many species have here their first description for the sampled region. The molecular analyses revealed the existence of a Barcode gap between the intra and the interspecific distances, although there was an overlap between the interespecific congeneric and intergeneric variation, prperties which hamper clear generic delimitation but stimulate DNA Barcode utilization for species designation. The phenograms/phylogenies obtained through the algorithms of Neighbor- Joining/Bayesian Inference also have shown that despite the reciprocal monophyly presented by the different species, the three genera were shown as polyphyletic within Drosophilidae. In this sense, we suggest here that the DNA Barcode technique is effective in the mycophagous species discrimination, but not in genera differentiation. In fact, the application of this technology for this group of species revealed straightforward utility in cryptic species discrimination when the analysis of morphological characters is not precise, mainly due to the exclusive existence of females. Moreover, our data show the importance of joining DNA Barcode with morphological data, which may help in the delimitation or even in the differentiation of likely new species. |
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2013-07-222013-07-222011-02-28BOLZAN, Andreza Ribeiro. DNA barcode of micophagous drosophilids comprising the genera Hirtodrosophila, Mycodrosophila e Zygothrica. 2011. 86 f. Dissertação (Mestrado em Ciencias Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/5281The biodiversity that exists in our planet is huge and far from being known. Most of the times the techniques that are used in species identification are based in the morphology of the specimens, and the description is a time-consuming work, that is limited to specialists. At contrast, DNA Barcode is intended to be a fast and accessible tool, which proposes the use of a standard DNA sequence encompassing the mitochondrial cytochrome c oxidase subunit I (COI) gene, with the aim of identifying species from already described sequences and discovering new species. Nevertheless, its efficiency is based on the diagnosis of specific properties, as monophyly of the intraspecific sequences and the existence of a Barcode gap between intra and interspecific variation. In this study, we tested the efficacy of the DNA Barcode in the identification/discovery of mycophagous drosofilid species belonging to the genera Hirtodrosophila, Mycodrosophila and Zygothrica. The specimens were collected throughout the southern Brazil, specially in the Santa Catarina and Rio Grande do Sul states, which totalized 10 collection points and 177 individuals. After morphological identification, total DNA was extracted and the COI (cytochrome oxidase c subunit I) and COII (cytochrome oxidase c subunit II genes) were amplified and sequenced, holding a total of 117 and 137 sequences, respectively, which were analyzed through phenetic and phylogenetic methods. A total of 33 different morphotypes were encountered and, with the collections it was possible to realize that the mycophagous genera Hirtodrosophila, Mycodrosophila and Zygothrica are well distributed throughout the Southern Brazilian Region. Furthermore, many species have here their first description for the sampled region. The molecular analyses revealed the existence of a Barcode gap between the intra and the interspecific distances, although there was an overlap between the interespecific congeneric and intergeneric variation, prperties which hamper clear generic delimitation but stimulate DNA Barcode utilization for species designation. The phenograms/phylogenies obtained through the algorithms of Neighbor- Joining/Bayesian Inference also have shown that despite the reciprocal monophyly presented by the different species, the three genera were shown as polyphyletic within Drosophilidae. In this sense, we suggest here that the DNA Barcode technique is effective in the mycophagous species discrimination, but not in genera differentiation. In fact, the application of this technology for this group of species revealed straightforward utility in cryptic species discrimination when the analysis of morphological characters is not precise, mainly due to the exclusive existence of females. Moreover, our data show the importance of joining DNA Barcode with morphological data, which may help in the delimitation or even in the differentiation of likely new species.A biodiversidade existente em nosso planeta é imensa e ainda está longe de ser conhecida. As técnicas utilizadas na identificação de espécies baseiam-se, muitas vezes, na morfologia dos espécimes, e sua descrição é um trabalho que demanda conhecimento e tempo, limitando-se à especialistas. Em contrapartida, com a intenção de ser uma ferramenta mais rápida e de fácil acesso, o DNA Barcode propõe o uso de uma sequência padronizada de DNA do gene mitocondrial citocromo c oxidase subunidade I (COI) com o objetivo de identificar espécimes a partir de espécies já descritas e descobrir novas espécies. Para sua efetividade, entretanto, é preciso que propriedades como o monofiletismo das sequências intraespecíficas e a existência de um gap entre as variações intra e interespecíficas sejam diagnosticadas. Neste trabalho, nós testamos a eficácia desta técnica para a identificação/descoberta de espécies de drosofilídeos micófagos dos gêneros Hirtodrosophila, Mycodrosophila e Zygothrica. Os espécimes foram obtidos a partir de coletas realizadas na região Sul do Brasil, em especial, nos estados de Santa Catarina e Rio Grande do Sul, totalizando 10 pontos de coleta e 177 indivíduos. Após a identificação morfológica, o DNA total foi extraído e os genes COI (citocromo c oxidase subunidade I) e COII (citocromo c oxidase subunidade II) foram amplificados e sequenciados, obtendo-se 117 e 137 sequências, respectivamente, que foram posteriormente analisadas através de métodos fenéticos e filogenéticos. Um total de 33 diferentes morfotipos foi encontrado e, pelas coletas, pode-se perceber que os gêneros micófagos Hirtodrosophila, Mycodrosophila e Zygothrica são bem distribuídos na região Sul. Muitas espécies, inclusive, têm aqui seu primeiro registro de coleta para a região. As análises moleculares revelaram a existência de um Barcode gap entre as distâncias intra e interespecíficas, porém com a presença de sobreposição das distâncias interespecíficas congenéricas e intergenéricas, propriedade que dificultam a clara delimitação dos gêneros, mas estimulam sua utilização para a designação das espécies. Os fenogramas/filogenias obtidos pelos algoritmos de Neighbor-Joining/Análise Bayesiana também mostraram que, a despeito da monofilia recíproca apresentada pelas diferentes espécies, os três gêneros apresentam-se polifiléticos dentro de Drosophilidae. Neste sentido, sugere-se aqui que a técnica seja efetiva na discriminação de espécies, mas não de gêneros diferentes. De fato, a aplicação da técnica de DNA Barcode para este grupo de organismos revelou a sua utilidade em auxiliar na discriminação entre espécies crípticas quando a análise de caracteres morfológicos não se faz precisa, principalmente pela existência exclusiva de fêmeas. Além disso, nossos dados demonstram a importância de agregar o DNA Barcode a dados de morfologia, o que pode auxiliar na deliminação ou até levar à diferenciação de prováveis espécies novas.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Biodiversidade AnimalUFSMBRCiências BiológicasDNA BarcodeDrosofilídeos micófagosDrosophilidaeHirtodrosophilaMycodrosophilaZygothricaDNA BarcodeMycophagous drosophilidsDrosophilidaeHirtodrosophilaMycodrosophilaZygothricaCNPQ::CIENCIAS BIOLOGICASDNA barcode de drosofilídeos micófagos pertencentes aos gêneros Hirtodrosophila, Mycodrosophila e ZygothricaDNA barcode of micophagous drosophilids comprising the genera Hirtodrosophila, Mycodrosophila e Zygothricainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisRobe, Lizandra Jaquelinehttp://lattes.cnpq.br/0384455492228279Blauth, Monica Lanerhttp://lattes.cnpq.br/2719193494210903Silva, Luciano Basso dahttp://lattes.cnpq.br/4485363387273456http://lattes.cnpq.br/8727370713454282Bolzan, Andreza Ribeiro20000000000640030030030030078dfac24-0ca4-421d-920e-c5093d53e9930ecb3cdd-359b-4255-99ba-b75d140519c707812804-b266-4c71-aa71-6bbd7a95a0a74aca4da6-e767-4e5b-b10b-477ee1c43161info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALBOLZAN, ANDREZA RIBEIRO.pdfapplication/pdf1441138http://repositorio.ufsm.br/bitstream/1/5281/1/BOLZAN%2c%20ANDREZA%20RIBEIRO.pdffe3076d5e0e2ad5536bf1aafeea7360eMD51TEXTBOLZAN, ANDREZA RIBEIRO.pdf.txtBOLZAN, ANDREZA RIBEIRO.pdf.txtExtracted texttext/plain152388http://repositorio.ufsm.br/bitstream/1/5281/2/BOLZAN%2c%20ANDREZA%20RIBEIRO.pdf.txt391114fcaacac9b2e825ed070472b291MD52THUMBNAILBOLZAN, ANDREZA RIBEIRO.pdf.jpgBOLZAN, ANDREZA RIBEIRO.pdf.jpgIM Thumbnailimage/jpeg4810http://repositorio.ufsm.br/bitstream/1/5281/3/BOLZAN%2c%20ANDREZA%20RIBEIRO.pdf.jpg2e108e01745f03593e918c42aaf169c8MD531/52812023-05-22 09:40:42.834oai:repositorio.ufsm.br:1/5281Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-05-22T12:40:42Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
DNA barcode de drosofilídeos micófagos pertencentes aos gêneros Hirtodrosophila, Mycodrosophila e Zygothrica |
dc.title.alternative.eng.fl_str_mv |
DNA barcode of micophagous drosophilids comprising the genera Hirtodrosophila, Mycodrosophila e Zygothrica |
title |
DNA barcode de drosofilídeos micófagos pertencentes aos gêneros Hirtodrosophila, Mycodrosophila e Zygothrica |
spellingShingle |
DNA barcode de drosofilídeos micófagos pertencentes aos gêneros Hirtodrosophila, Mycodrosophila e Zygothrica Bolzan, Andreza Ribeiro DNA Barcode Drosofilídeos micófagos Drosophilidae Hirtodrosophila Mycodrosophila Zygothrica DNA Barcode Mycophagous drosophilids Drosophilidae Hirtodrosophila Mycodrosophila Zygothrica CNPQ::CIENCIAS BIOLOGICAS |
title_short |
DNA barcode de drosofilídeos micófagos pertencentes aos gêneros Hirtodrosophila, Mycodrosophila e Zygothrica |
title_full |
DNA barcode de drosofilídeos micófagos pertencentes aos gêneros Hirtodrosophila, Mycodrosophila e Zygothrica |
title_fullStr |
DNA barcode de drosofilídeos micófagos pertencentes aos gêneros Hirtodrosophila, Mycodrosophila e Zygothrica |
title_full_unstemmed |
DNA barcode de drosofilídeos micófagos pertencentes aos gêneros Hirtodrosophila, Mycodrosophila e Zygothrica |
title_sort |
DNA barcode de drosofilídeos micófagos pertencentes aos gêneros Hirtodrosophila, Mycodrosophila e Zygothrica |
author |
Bolzan, Andreza Ribeiro |
author_facet |
Bolzan, Andreza Ribeiro |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Robe, Lizandra Jaqueline |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0384455492228279 |
dc.contributor.referee1.fl_str_mv |
Blauth, Monica Laner |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2719193494210903 |
dc.contributor.referee2.fl_str_mv |
Silva, Luciano Basso da |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4485363387273456 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8727370713454282 |
dc.contributor.author.fl_str_mv |
Bolzan, Andreza Ribeiro |
contributor_str_mv |
Robe, Lizandra Jaqueline Blauth, Monica Laner Silva, Luciano Basso da |
dc.subject.por.fl_str_mv |
DNA Barcode Drosofilídeos micófagos Drosophilidae Hirtodrosophila Mycodrosophila Zygothrica |
topic |
DNA Barcode Drosofilídeos micófagos Drosophilidae Hirtodrosophila Mycodrosophila Zygothrica DNA Barcode Mycophagous drosophilids Drosophilidae Hirtodrosophila Mycodrosophila Zygothrica CNPQ::CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
DNA Barcode Mycophagous drosophilids Drosophilidae Hirtodrosophila Mycodrosophila Zygothrica |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS |
description |
The biodiversity that exists in our planet is huge and far from being known. Most of the times the techniques that are used in species identification are based in the morphology of the specimens, and the description is a time-consuming work, that is limited to specialists. At contrast, DNA Barcode is intended to be a fast and accessible tool, which proposes the use of a standard DNA sequence encompassing the mitochondrial cytochrome c oxidase subunit I (COI) gene, with the aim of identifying species from already described sequences and discovering new species. Nevertheless, its efficiency is based on the diagnosis of specific properties, as monophyly of the intraspecific sequences and the existence of a Barcode gap between intra and interspecific variation. In this study, we tested the efficacy of the DNA Barcode in the identification/discovery of mycophagous drosofilid species belonging to the genera Hirtodrosophila, Mycodrosophila and Zygothrica. The specimens were collected throughout the southern Brazil, specially in the Santa Catarina and Rio Grande do Sul states, which totalized 10 collection points and 177 individuals. After morphological identification, total DNA was extracted and the COI (cytochrome oxidase c subunit I) and COII (cytochrome oxidase c subunit II genes) were amplified and sequenced, holding a total of 117 and 137 sequences, respectively, which were analyzed through phenetic and phylogenetic methods. A total of 33 different morphotypes were encountered and, with the collections it was possible to realize that the mycophagous genera Hirtodrosophila, Mycodrosophila and Zygothrica are well distributed throughout the Southern Brazilian Region. Furthermore, many species have here their first description for the sampled region. The molecular analyses revealed the existence of a Barcode gap between the intra and the interspecific distances, although there was an overlap between the interespecific congeneric and intergeneric variation, prperties which hamper clear generic delimitation but stimulate DNA Barcode utilization for species designation. The phenograms/phylogenies obtained through the algorithms of Neighbor- Joining/Bayesian Inference also have shown that despite the reciprocal monophyly presented by the different species, the three genera were shown as polyphyletic within Drosophilidae. In this sense, we suggest here that the DNA Barcode technique is effective in the mycophagous species discrimination, but not in genera differentiation. In fact, the application of this technology for this group of species revealed straightforward utility in cryptic species discrimination when the analysis of morphological characters is not precise, mainly due to the exclusive existence of females. Moreover, our data show the importance of joining DNA Barcode with morphological data, which may help in the delimitation or even in the differentiation of likely new species. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-28 |
dc.date.accessioned.fl_str_mv |
2013-07-22 |
dc.date.available.fl_str_mv |
2013-07-22 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
BOLZAN, Andreza Ribeiro. DNA barcode of micophagous drosophilids comprising the genera Hirtodrosophila, Mycodrosophila e Zygothrica. 2011. 86 f. Dissertação (Mestrado em Ciencias Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/5281 |
identifier_str_mv |
BOLZAN, Andreza Ribeiro. DNA barcode of micophagous drosophilids comprising the genera Hirtodrosophila, Mycodrosophila e Zygothrica. 2011. 86 f. Dissertação (Mestrado em Ciencias Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
url |
http://repositorio.ufsm.br/handle/1/5281 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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200000000006 |
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400 300 300 300 300 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
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Programa de Pós-Graduação em Biodiversidade Animal |
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UFSM |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Ciências Biológicas |
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Universidade Federal de Santa Maria |
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