Resposta da microalga clorofícea Desmodesmus quadricauda (Turpin) Brébisson a acrilamida em diferentes condições simuladas de estresse abiótico

• Autor(a) principal: Elicker, Camila
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Manancial - Repositório Digital da UFSM
• dARK ID: ark:/26339/001300000pgds
• Texto Completo: http://repositorio.ufsm.br/handle/1/4913
• topic: Ecotoxicologia; Clorofíceas; Biovolume; Ecotoxicology; Chlorophytes; Biovolume; CNPQ::CIENCIAS BIOLOGICAS

Essential to life, water is present in almost all human activities, mainly in the consumption of drinking water and agricultural activities. Considering that the world population has increased enormously, food production must be increased and this need has encouraged cooperatives and producers to seek alternative techniques to improve productivity and reduce costs. In this context, the addition of hydrogels on the ground, optimizes the availability of water due to its large storage capacity, accelerating the development of the root system and aerial part of the plant. Most hydrogels is done based on polyacrylamide polymer of acrylamide (AM) monomer which is in the form odorless and white colored water soluble and highly toxic. The damaging effects of AM, which has carcinogenic risk and endocrine disrupter in living beings are known for over 30 years. In soil, acrylamide has a high time to be degraded and due to leaching, the monomer can be found in aquatic environments. Due to its toxicity, acrylamide in a concentration of 5.88 mg / L-1 restates the EC50 microalgae Chlorophyceae Desmodesmus quadricauda (Turpin) Brébisson. In addition to the influence of pollution, various abiotic factors such as temperature and pH, interfere with aquatic communities. The aim of this study was to evaluate the toxic effects of acrylamide in predetermined concentration with changes in temperature (24 ° C and 17 ° C) and pH variation on physiological parameters of microalgae D. quadricauda. The green algae were grown in Water Culture (WC) with its exposed cells in exponential growth phase for a period of 72 hours at concentrations of 5.88 mg l-1 of acrylamide, pH values ranging from 5, 6, 7 and 8. Periodic samples counts were performed under the microscope, in which it took into account the phenotypic plasticity and cellular measures, the biovolume and cell density. When comparing this study with acrylamide EC50 obtained in a previous study, the results indicated at low temperatures (17 ° C) the toxicity of acrylamide and decreased at high temperatures (24 ° C) increased, not having the pH interfered in this parameter. Analyzing cellular measures of width and length, results were significant with the control of pH 8 and temperature 15 ° C, as in biovolume values where only the variation of pH shows variation among the tested controls and treatments. Phenotypic gender plasticity shows that when analyzing the groups separately for periods of 24, 48 and 72 hours, although no significant differences, most phenotypic change was found in the monasteries of a cell that prevailed most in the group treated with acrylamide and pH change in almost all periods. Therefore, the concentration of acrylamide in this test along with changes in temperature and pH tested, indicating that the toxicity of the substance changes the parameters tested in microalgae, becoming a risk to aquatic communities exposed to acrylamide.