Produção de ácido láctico a partir da casca de arroz

Detalhes bibliográficos
Autor(a) principal: Montipó, Sheila
Data de Publicação: 2012
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000qdnh
Texto Completo: http://repositorio.ufsm.br/handle/1/7961
Resumo: Lignocellulosic agroindustrial wastes, such as rice husks (RH), are abundant, low cost, renewable sources, available to biotransformation in value-added products. Thus, the main objective of this study was to investigate the use of RH with the purpose of its biotransformation in lactic acid (LacA) starting from hydrolysates of this residual biomass. At the same time, aiming to contribute to the mitigation of the growing environmental concern about the illegal disposal of this waste in the State of Rio Grande do Sul the biggest Brazilian rice producer. For this, a central composite rotational design (CCRD) of the hydrolysis experiments of RH in pressurized conversion system with diluted HCl and H2SO4, revealed that experiments conducted at 175 °C, for 46 min, with acid concentrations of 2.2% v v-1, generate the highest glucose contents. For hydrolysis involving HCl, 17.8 g glucose L-1, with an yield of 106.6 mg glucose g-1 RH; for hydrolysis with H2SO4, 14.1 g glucose L-1, with an yield of 84.5 mg glucose g-1 RH. Previous fermentative experiments were carried out with the aim to select resilient and efficient micro-organisms for the LacA production and, also, to evaluate the need of supplementation of the RH hydrolyzate with specific nutrients. L. rhamnosus, in this study, was the most suitable bacteria for the lactic fermentation, among all the Lactobacillus tested, being able to to produce 5.6 g LacA L-1, with a yield of 33.8 mg LacA g-1 RH (hydrolysed with HCl, without nutrient addition, 96 h fermentation). The acid hydrolysis, in this particular case, was carried out at 160 °C, for 70 min, using 4.0% HCl v v-1, generating 14.7 g glucose L-1. The lactic fermentation via CCRD was batch conducted in 4 mL vials, at 37 °C, pH 6.0, using L. casei subsp. rhamnosus ATCC 10863, specialized in catabolization of both hexoses and pentoses (except arabinose). Experiments with RH hydrolysates containing nutrients added produced up to 18.8 g LacA L-1 (maximum yield of 997,1 mg LacA g-1 sugars) in substrate hydrolyzed with HCl, after 52 h fermentation; and 14.6 g LacA L-1 (maximum yield of 999.8 mg LacA g-1 sugars) in substrate hydrolyzed with H2SO4, after 24 h fermentation. Therefore, in this study, the choices of most influence in the LacA production were fermentation with sulfuric acid and employment of L. rhamnosus.
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spelling Produção de ácido láctico a partir da casca de arrozLactic acid production from rice huskBiorrefinariaCasca de arrozHidrólise ácida. LCasei subsp. rhamnosus ATCC 10863Ácido lácticoOtimização de processosCromatografiaBiorefineryRice husksAcid hydrolysisLactic acidProcess optimizationChromatographyCNPQ::ENGENHARIAS::ENGENHARIA DE PRODUCAOLignocellulosic agroindustrial wastes, such as rice husks (RH), are abundant, low cost, renewable sources, available to biotransformation in value-added products. Thus, the main objective of this study was to investigate the use of RH with the purpose of its biotransformation in lactic acid (LacA) starting from hydrolysates of this residual biomass. At the same time, aiming to contribute to the mitigation of the growing environmental concern about the illegal disposal of this waste in the State of Rio Grande do Sul the biggest Brazilian rice producer. For this, a central composite rotational design (CCRD) of the hydrolysis experiments of RH in pressurized conversion system with diluted HCl and H2SO4, revealed that experiments conducted at 175 °C, for 46 min, with acid concentrations of 2.2% v v-1, generate the highest glucose contents. For hydrolysis involving HCl, 17.8 g glucose L-1, with an yield of 106.6 mg glucose g-1 RH; for hydrolysis with H2SO4, 14.1 g glucose L-1, with an yield of 84.5 mg glucose g-1 RH. Previous fermentative experiments were carried out with the aim to select resilient and efficient micro-organisms for the LacA production and, also, to evaluate the need of supplementation of the RH hydrolyzate with specific nutrients. L. rhamnosus, in this study, was the most suitable bacteria for the lactic fermentation, among all the Lactobacillus tested, being able to to produce 5.6 g LacA L-1, with a yield of 33.8 mg LacA g-1 RH (hydrolysed with HCl, without nutrient addition, 96 h fermentation). The acid hydrolysis, in this particular case, was carried out at 160 °C, for 70 min, using 4.0% HCl v v-1, generating 14.7 g glucose L-1. The lactic fermentation via CCRD was batch conducted in 4 mL vials, at 37 °C, pH 6.0, using L. casei subsp. rhamnosus ATCC 10863, specialized in catabolization of both hexoses and pentoses (except arabinose). Experiments with RH hydrolysates containing nutrients added produced up to 18.8 g LacA L-1 (maximum yield of 997,1 mg LacA g-1 sugars) in substrate hydrolyzed with HCl, after 52 h fermentation; and 14.6 g LacA L-1 (maximum yield of 999.8 mg LacA g-1 sugars) in substrate hydrolyzed with H2SO4, after 24 h fermentation. Therefore, in this study, the choices of most influence in the LacA production were fermentation with sulfuric acid and employment of L. rhamnosus.Conselho Nacional de Desenvolvimento Científico e TecnológicoResíduos lignocelulósicos agroindustriais, como a casca de arroz (CA), são fontes renováveis, abundantes e de baixo custo, disponíveis para a biotransformação em produtos com valor agregado. O principal objetivo deste trabalho foi investigar o aproveitamento da CA com vistas à sua biotransformação em ácido láctico (ALac), partindo-se de hidrolisados desta biomassa residual. Ao mesmo tempo, objetivou-se contribuir para a mitigação do crescente problema ambiental decorrente da disposição irregular deste resíduo no estado do Rio Grande do Sul, maior produtor brasileiro. Para tanto, o delineamento composto central rotacional (DCCR) do planejamento experimental da hidrólise ácida da CA em sistema de conversão à pressão, com HCl e H2SO4 diluídos, revelou que, experimentos conduzidos a 175 °C, por 46 min, com concentração de 2,2% (v v-1) de ácido, produziram os teores máximos de glicose. Para hidrólises envolvendo HCl, teor de 17,8 g de glicose L-1, com rendimento de 106,6 mg de glicose g-1 CA; para hidrólises com H2SO4, teor de 14,1 g de glicose L-1, com rendimento de 84,5 mg de glicose g-1 CA. Ensaios fermentativos prévios foram feitos com o intuito de selecionar micro-organismos resilientes e eficientes para a produção de ALac e, também, para avaliar a necessidade de suplementação do hidrolisado da CA com nutrientes específicos. L. rhamnosus, neste trabalho, foi a bactéria mais apropriada à fermentação láctica, dentre os Lactobacillus testados, sendo capaz de produzir 5,6 g ALac L-1, com rendimento de 33,8 mg ALac g-1 CA (hidrolisado com HCl, sem adição de nutrientes, 96 h de fermentação). A hidrólise ácida, neste caso específico, foi feita a 160 °C, por 70 min, com 4,0% HCl v v-1, gerando 14,7 g L-1 de glicose. A fermentação láctica via DCCR foi conduzida em vials de 4 mL, em batelada, a 37 °C, pH 6,0, empregando-se L. casei subsp. rhamnosus ATCC 10863, cepa especializada em catabolização de hexoses e pentoses (exceto arabinose). Experimentos com hidrolisados da CA adicionados de nutrientes produziram até 18,8 g ALac L-1 (rendimento máximo de 997,1 mg ALac g-1 açúcares) em substrato hidrolisado com HCl, após 52 h de fermentação; e 14,6 g ALac L-1 (rendimento máximo de 999,8 mg ALac g-1 açúcares) em substrato hidrolisado com H2SO4, após 24 h de fermentação. Portanto, neste trabalho, as escolhas de maior influência na produção de ALac foram a fermentação com hidrolisado sulfúrico e emprego de L. rhamnosus.Universidade Federal de Santa MariaBREngenharia de ProcessosUFSMPrograma de Pós-Graduação em Engenharia de ProcessosMartins, Ayrton Figueiredohttp://lattes.cnpq.br/2113532494494821Mazutti, Marcio Antoniohttp://lattes.cnpq.br/3520282081196395Camassola, Marlihttp://lattes.cnpq.br/0418316745860676Montipó, Sheila2014-01-232014-01-232012-11-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfMONTIPÓ, Sheila. Lactic acid production from rice husk. 2012. 100 f. Dissertação (Mestrado em Engenharia) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/7961ark:/26339/001300000qdnhporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-01-04T13:37:00Zoai:repositorio.ufsm.br:1/7961Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-01-04T13:37Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Produção de ácido láctico a partir da casca de arroz
Lactic acid production from rice husk
title Produção de ácido láctico a partir da casca de arroz
spellingShingle Produção de ácido láctico a partir da casca de arroz
Montipó, Sheila
Biorrefinaria
Casca de arroz
Hidrólise ácida. L
Casei subsp. rhamnosus ATCC 10863
Ácido láctico
Otimização de processos
Cromatografia
Biorefinery
Rice husks
Acid hydrolysis
Lactic acid
Process optimization
Chromatography
CNPQ::ENGENHARIAS::ENGENHARIA DE PRODUCAO
title_short Produção de ácido láctico a partir da casca de arroz
title_full Produção de ácido láctico a partir da casca de arroz
title_fullStr Produção de ácido láctico a partir da casca de arroz
title_full_unstemmed Produção de ácido láctico a partir da casca de arroz
title_sort Produção de ácido láctico a partir da casca de arroz
author Montipó, Sheila
author_facet Montipó, Sheila
author_role author
dc.contributor.none.fl_str_mv Martins, Ayrton Figueiredo
http://lattes.cnpq.br/2113532494494821
Mazutti, Marcio Antonio
http://lattes.cnpq.br/3520282081196395
Camassola, Marli
http://lattes.cnpq.br/0418316745860676
dc.contributor.author.fl_str_mv Montipó, Sheila
dc.subject.por.fl_str_mv Biorrefinaria
Casca de arroz
Hidrólise ácida. L
Casei subsp. rhamnosus ATCC 10863
Ácido láctico
Otimização de processos
Cromatografia
Biorefinery
Rice husks
Acid hydrolysis
Lactic acid
Process optimization
Chromatography
CNPQ::ENGENHARIAS::ENGENHARIA DE PRODUCAO
topic Biorrefinaria
Casca de arroz
Hidrólise ácida. L
Casei subsp. rhamnosus ATCC 10863
Ácido láctico
Otimização de processos
Cromatografia
Biorefinery
Rice husks
Acid hydrolysis
Lactic acid
Process optimization
Chromatography
CNPQ::ENGENHARIAS::ENGENHARIA DE PRODUCAO
description Lignocellulosic agroindustrial wastes, such as rice husks (RH), are abundant, low cost, renewable sources, available to biotransformation in value-added products. Thus, the main objective of this study was to investigate the use of RH with the purpose of its biotransformation in lactic acid (LacA) starting from hydrolysates of this residual biomass. At the same time, aiming to contribute to the mitigation of the growing environmental concern about the illegal disposal of this waste in the State of Rio Grande do Sul the biggest Brazilian rice producer. For this, a central composite rotational design (CCRD) of the hydrolysis experiments of RH in pressurized conversion system with diluted HCl and H2SO4, revealed that experiments conducted at 175 °C, for 46 min, with acid concentrations of 2.2% v v-1, generate the highest glucose contents. For hydrolysis involving HCl, 17.8 g glucose L-1, with an yield of 106.6 mg glucose g-1 RH; for hydrolysis with H2SO4, 14.1 g glucose L-1, with an yield of 84.5 mg glucose g-1 RH. Previous fermentative experiments were carried out with the aim to select resilient and efficient micro-organisms for the LacA production and, also, to evaluate the need of supplementation of the RH hydrolyzate with specific nutrients. L. rhamnosus, in this study, was the most suitable bacteria for the lactic fermentation, among all the Lactobacillus tested, being able to to produce 5.6 g LacA L-1, with a yield of 33.8 mg LacA g-1 RH (hydrolysed with HCl, without nutrient addition, 96 h fermentation). The acid hydrolysis, in this particular case, was carried out at 160 °C, for 70 min, using 4.0% HCl v v-1, generating 14.7 g glucose L-1. The lactic fermentation via CCRD was batch conducted in 4 mL vials, at 37 °C, pH 6.0, using L. casei subsp. rhamnosus ATCC 10863, specialized in catabolization of both hexoses and pentoses (except arabinose). Experiments with RH hydrolysates containing nutrients added produced up to 18.8 g LacA L-1 (maximum yield of 997,1 mg LacA g-1 sugars) in substrate hydrolyzed with HCl, after 52 h fermentation; and 14.6 g LacA L-1 (maximum yield of 999.8 mg LacA g-1 sugars) in substrate hydrolyzed with H2SO4, after 24 h fermentation. Therefore, in this study, the choices of most influence in the LacA production were fermentation with sulfuric acid and employment of L. rhamnosus.
publishDate 2012
dc.date.none.fl_str_mv 2012-11-16
2014-01-23
2014-01-23
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv MONTIPÓ, Sheila. Lactic acid production from rice husk. 2012. 100 f. Dissertação (Mestrado em Engenharia) - Universidade Federal de Santa Maria, Santa Maria, 2012.
http://repositorio.ufsm.br/handle/1/7961
dc.identifier.dark.fl_str_mv ark:/26339/001300000qdnh
identifier_str_mv MONTIPÓ, Sheila. Lactic acid production from rice husk. 2012. 100 f. Dissertação (Mestrado em Engenharia) - Universidade Federal de Santa Maria, Santa Maria, 2012.
ark:/26339/001300000qdnh
url http://repositorio.ufsm.br/handle/1/7961
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Engenharia de Processos
UFSM
Programa de Pós-Graduação em Engenharia de Processos
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Engenharia de Processos
UFSM
Programa de Pós-Graduação em Engenharia de Processos
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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