Produção de ácido láctico a partir da casca de arroz
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
dARK ID: | ark:/26339/001300000qdnh |
Texto Completo: | http://repositorio.ufsm.br/handle/1/7961 |
Resumo: | Lignocellulosic agroindustrial wastes, such as rice husks (RH), are abundant, low cost, renewable sources, available to biotransformation in value-added products. Thus, the main objective of this study was to investigate the use of RH with the purpose of its biotransformation in lactic acid (LacA) starting from hydrolysates of this residual biomass. At the same time, aiming to contribute to the mitigation of the growing environmental concern about the illegal disposal of this waste in the State of Rio Grande do Sul the biggest Brazilian rice producer. For this, a central composite rotational design (CCRD) of the hydrolysis experiments of RH in pressurized conversion system with diluted HCl and H2SO4, revealed that experiments conducted at 175 °C, for 46 min, with acid concentrations of 2.2% v v-1, generate the highest glucose contents. For hydrolysis involving HCl, 17.8 g glucose L-1, with an yield of 106.6 mg glucose g-1 RH; for hydrolysis with H2SO4, 14.1 g glucose L-1, with an yield of 84.5 mg glucose g-1 RH. Previous fermentative experiments were carried out with the aim to select resilient and efficient micro-organisms for the LacA production and, also, to evaluate the need of supplementation of the RH hydrolyzate with specific nutrients. L. rhamnosus, in this study, was the most suitable bacteria for the lactic fermentation, among all the Lactobacillus tested, being able to to produce 5.6 g LacA L-1, with a yield of 33.8 mg LacA g-1 RH (hydrolysed with HCl, without nutrient addition, 96 h fermentation). The acid hydrolysis, in this particular case, was carried out at 160 °C, for 70 min, using 4.0% HCl v v-1, generating 14.7 g glucose L-1. The lactic fermentation via CCRD was batch conducted in 4 mL vials, at 37 °C, pH 6.0, using L. casei subsp. rhamnosus ATCC 10863, specialized in catabolization of both hexoses and pentoses (except arabinose). Experiments with RH hydrolysates containing nutrients added produced up to 18.8 g LacA L-1 (maximum yield of 997,1 mg LacA g-1 sugars) in substrate hydrolyzed with HCl, after 52 h fermentation; and 14.6 g LacA L-1 (maximum yield of 999.8 mg LacA g-1 sugars) in substrate hydrolyzed with H2SO4, after 24 h fermentation. Therefore, in this study, the choices of most influence in the LacA production were fermentation with sulfuric acid and employment of L. rhamnosus. |
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Produção de ácido láctico a partir da casca de arrozLactic acid production from rice huskBiorrefinariaCasca de arrozHidrólise ácida. LCasei subsp. rhamnosus ATCC 10863Ácido lácticoOtimização de processosCromatografiaBiorefineryRice husksAcid hydrolysisLactic acidProcess optimizationChromatographyCNPQ::ENGENHARIAS::ENGENHARIA DE PRODUCAOLignocellulosic agroindustrial wastes, such as rice husks (RH), are abundant, low cost, renewable sources, available to biotransformation in value-added products. Thus, the main objective of this study was to investigate the use of RH with the purpose of its biotransformation in lactic acid (LacA) starting from hydrolysates of this residual biomass. At the same time, aiming to contribute to the mitigation of the growing environmental concern about the illegal disposal of this waste in the State of Rio Grande do Sul the biggest Brazilian rice producer. For this, a central composite rotational design (CCRD) of the hydrolysis experiments of RH in pressurized conversion system with diluted HCl and H2SO4, revealed that experiments conducted at 175 °C, for 46 min, with acid concentrations of 2.2% v v-1, generate the highest glucose contents. For hydrolysis involving HCl, 17.8 g glucose L-1, with an yield of 106.6 mg glucose g-1 RH; for hydrolysis with H2SO4, 14.1 g glucose L-1, with an yield of 84.5 mg glucose g-1 RH. Previous fermentative experiments were carried out with the aim to select resilient and efficient micro-organisms for the LacA production and, also, to evaluate the need of supplementation of the RH hydrolyzate with specific nutrients. L. rhamnosus, in this study, was the most suitable bacteria for the lactic fermentation, among all the Lactobacillus tested, being able to to produce 5.6 g LacA L-1, with a yield of 33.8 mg LacA g-1 RH (hydrolysed with HCl, without nutrient addition, 96 h fermentation). The acid hydrolysis, in this particular case, was carried out at 160 °C, for 70 min, using 4.0% HCl v v-1, generating 14.7 g glucose L-1. The lactic fermentation via CCRD was batch conducted in 4 mL vials, at 37 °C, pH 6.0, using L. casei subsp. rhamnosus ATCC 10863, specialized in catabolization of both hexoses and pentoses (except arabinose). Experiments with RH hydrolysates containing nutrients added produced up to 18.8 g LacA L-1 (maximum yield of 997,1 mg LacA g-1 sugars) in substrate hydrolyzed with HCl, after 52 h fermentation; and 14.6 g LacA L-1 (maximum yield of 999.8 mg LacA g-1 sugars) in substrate hydrolyzed with H2SO4, after 24 h fermentation. Therefore, in this study, the choices of most influence in the LacA production were fermentation with sulfuric acid and employment of L. rhamnosus.Conselho Nacional de Desenvolvimento Científico e TecnológicoResíduos lignocelulósicos agroindustriais, como a casca de arroz (CA), são fontes renováveis, abundantes e de baixo custo, disponíveis para a biotransformação em produtos com valor agregado. O principal objetivo deste trabalho foi investigar o aproveitamento da CA com vistas à sua biotransformação em ácido láctico (ALac), partindo-se de hidrolisados desta biomassa residual. Ao mesmo tempo, objetivou-se contribuir para a mitigação do crescente problema ambiental decorrente da disposição irregular deste resíduo no estado do Rio Grande do Sul, maior produtor brasileiro. Para tanto, o delineamento composto central rotacional (DCCR) do planejamento experimental da hidrólise ácida da CA em sistema de conversão à pressão, com HCl e H2SO4 diluídos, revelou que, experimentos conduzidos a 175 °C, por 46 min, com concentração de 2,2% (v v-1) de ácido, produziram os teores máximos de glicose. Para hidrólises envolvendo HCl, teor de 17,8 g de glicose L-1, com rendimento de 106,6 mg de glicose g-1 CA; para hidrólises com H2SO4, teor de 14,1 g de glicose L-1, com rendimento de 84,5 mg de glicose g-1 CA. Ensaios fermentativos prévios foram feitos com o intuito de selecionar micro-organismos resilientes e eficientes para a produção de ALac e, também, para avaliar a necessidade de suplementação do hidrolisado da CA com nutrientes específicos. L. rhamnosus, neste trabalho, foi a bactéria mais apropriada à fermentação láctica, dentre os Lactobacillus testados, sendo capaz de produzir 5,6 g ALac L-1, com rendimento de 33,8 mg ALac g-1 CA (hidrolisado com HCl, sem adição de nutrientes, 96 h de fermentação). A hidrólise ácida, neste caso específico, foi feita a 160 °C, por 70 min, com 4,0% HCl v v-1, gerando 14,7 g L-1 de glicose. A fermentação láctica via DCCR foi conduzida em vials de 4 mL, em batelada, a 37 °C, pH 6,0, empregando-se L. casei subsp. rhamnosus ATCC 10863, cepa especializada em catabolização de hexoses e pentoses (exceto arabinose). Experimentos com hidrolisados da CA adicionados de nutrientes produziram até 18,8 g ALac L-1 (rendimento máximo de 997,1 mg ALac g-1 açúcares) em substrato hidrolisado com HCl, após 52 h de fermentação; e 14,6 g ALac L-1 (rendimento máximo de 999,8 mg ALac g-1 açúcares) em substrato hidrolisado com H2SO4, após 24 h de fermentação. Portanto, neste trabalho, as escolhas de maior influência na produção de ALac foram a fermentação com hidrolisado sulfúrico e emprego de L. rhamnosus.Universidade Federal de Santa MariaBREngenharia de ProcessosUFSMPrograma de Pós-Graduação em Engenharia de ProcessosMartins, Ayrton Figueiredohttp://lattes.cnpq.br/2113532494494821Mazutti, Marcio Antoniohttp://lattes.cnpq.br/3520282081196395Camassola, Marlihttp://lattes.cnpq.br/0418316745860676Montipó, Sheila2014-01-232014-01-232012-11-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfMONTIPÓ, Sheila. Lactic acid production from rice husk. 2012. 100 f. Dissertação (Mestrado em Engenharia) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/7961ark:/26339/001300000qdnhporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-01-04T13:37:00Zoai:repositorio.ufsm.br:1/7961Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-01-04T13:37Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Produção de ácido láctico a partir da casca de arroz Lactic acid production from rice husk |
title |
Produção de ácido láctico a partir da casca de arroz |
spellingShingle |
Produção de ácido láctico a partir da casca de arroz Montipó, Sheila Biorrefinaria Casca de arroz Hidrólise ácida. L Casei subsp. rhamnosus ATCC 10863 Ácido láctico Otimização de processos Cromatografia Biorefinery Rice husks Acid hydrolysis Lactic acid Process optimization Chromatography CNPQ::ENGENHARIAS::ENGENHARIA DE PRODUCAO |
title_short |
Produção de ácido láctico a partir da casca de arroz |
title_full |
Produção de ácido láctico a partir da casca de arroz |
title_fullStr |
Produção de ácido láctico a partir da casca de arroz |
title_full_unstemmed |
Produção de ácido láctico a partir da casca de arroz |
title_sort |
Produção de ácido láctico a partir da casca de arroz |
author |
Montipó, Sheila |
author_facet |
Montipó, Sheila |
author_role |
author |
dc.contributor.none.fl_str_mv |
Martins, Ayrton Figueiredo http://lattes.cnpq.br/2113532494494821 Mazutti, Marcio Antonio http://lattes.cnpq.br/3520282081196395 Camassola, Marli http://lattes.cnpq.br/0418316745860676 |
dc.contributor.author.fl_str_mv |
Montipó, Sheila |
dc.subject.por.fl_str_mv |
Biorrefinaria Casca de arroz Hidrólise ácida. L Casei subsp. rhamnosus ATCC 10863 Ácido láctico Otimização de processos Cromatografia Biorefinery Rice husks Acid hydrolysis Lactic acid Process optimization Chromatography CNPQ::ENGENHARIAS::ENGENHARIA DE PRODUCAO |
topic |
Biorrefinaria Casca de arroz Hidrólise ácida. L Casei subsp. rhamnosus ATCC 10863 Ácido láctico Otimização de processos Cromatografia Biorefinery Rice husks Acid hydrolysis Lactic acid Process optimization Chromatography CNPQ::ENGENHARIAS::ENGENHARIA DE PRODUCAO |
description |
Lignocellulosic agroindustrial wastes, such as rice husks (RH), are abundant, low cost, renewable sources, available to biotransformation in value-added products. Thus, the main objective of this study was to investigate the use of RH with the purpose of its biotransformation in lactic acid (LacA) starting from hydrolysates of this residual biomass. At the same time, aiming to contribute to the mitigation of the growing environmental concern about the illegal disposal of this waste in the State of Rio Grande do Sul the biggest Brazilian rice producer. For this, a central composite rotational design (CCRD) of the hydrolysis experiments of RH in pressurized conversion system with diluted HCl and H2SO4, revealed that experiments conducted at 175 °C, for 46 min, with acid concentrations of 2.2% v v-1, generate the highest glucose contents. For hydrolysis involving HCl, 17.8 g glucose L-1, with an yield of 106.6 mg glucose g-1 RH; for hydrolysis with H2SO4, 14.1 g glucose L-1, with an yield of 84.5 mg glucose g-1 RH. Previous fermentative experiments were carried out with the aim to select resilient and efficient micro-organisms for the LacA production and, also, to evaluate the need of supplementation of the RH hydrolyzate with specific nutrients. L. rhamnosus, in this study, was the most suitable bacteria for the lactic fermentation, among all the Lactobacillus tested, being able to to produce 5.6 g LacA L-1, with a yield of 33.8 mg LacA g-1 RH (hydrolysed with HCl, without nutrient addition, 96 h fermentation). The acid hydrolysis, in this particular case, was carried out at 160 °C, for 70 min, using 4.0% HCl v v-1, generating 14.7 g glucose L-1. The lactic fermentation via CCRD was batch conducted in 4 mL vials, at 37 °C, pH 6.0, using L. casei subsp. rhamnosus ATCC 10863, specialized in catabolization of both hexoses and pentoses (except arabinose). Experiments with RH hydrolysates containing nutrients added produced up to 18.8 g LacA L-1 (maximum yield of 997,1 mg LacA g-1 sugars) in substrate hydrolyzed with HCl, after 52 h fermentation; and 14.6 g LacA L-1 (maximum yield of 999.8 mg LacA g-1 sugars) in substrate hydrolyzed with H2SO4, after 24 h fermentation. Therefore, in this study, the choices of most influence in the LacA production were fermentation with sulfuric acid and employment of L. rhamnosus. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-16 2014-01-23 2014-01-23 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MONTIPÓ, Sheila. Lactic acid production from rice husk. 2012. 100 f. Dissertação (Mestrado em Engenharia) - Universidade Federal de Santa Maria, Santa Maria, 2012. http://repositorio.ufsm.br/handle/1/7961 |
dc.identifier.dark.fl_str_mv |
ark:/26339/001300000qdnh |
identifier_str_mv |
MONTIPÓ, Sheila. Lactic acid production from rice husk. 2012. 100 f. Dissertação (Mestrado em Engenharia) - Universidade Federal de Santa Maria, Santa Maria, 2012. ark:/26339/001300000qdnh |
url |
http://repositorio.ufsm.br/handle/1/7961 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Engenharia de Processos UFSM Programa de Pós-Graduação em Engenharia de Processos |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Engenharia de Processos UFSM Programa de Pós-Graduação em Engenharia de Processos |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1815172378870677504 |