Notes total e híbrida em frangos (Gallus gallus domesticus) como modelo experimental para aves exóticas
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/21284 |
Resumo: | The purpose of this study was to assess the feasibility of the intracloacal hybrid-NOTES (HN) or total-NOTES (TN) techniques in chickens (Gallus gallus domesticus) for biopsy sampling, in two experimental stages: (1) to assess the HN and TN techniques in chicken cadavers for liver and kidney biopsy, regarding the quality of access, complications and general findings; (2) to compare the HN or TN technique for liver biopsy in posture pullets regarding operative time, complications, technical difficulties and histological quality of liver samples. In the first stage of the study, four cadavers of broiler chicken, weighting 2.6 (± 0.7) kg. Both HN and TN surgical approaches for biopsy sampling were performed in all cadavers. Surgical steps were classified into: (1) surgical access, which involved cloacoscopy and endoscope entry into the celomatic cavity. This step was common to both treatments; (2) HN liver and renal biopsy sampling, by insertion of a 5-fr. biopsy forceps through a percutaneous 14G catheter sheath; (3) TN liver and renal biopsy sampling, by insertion of the biopsy forceps through the same cloacal access, in parallel to the endoscope; (4) cloacal synthesis. Both surgical approaches were successfully carried out in all cadavers (n = 4). Time for the surgical steps were 2.7 (± 1.4) min for surgical access, 9.2 (± 3.4) minutes for HN biopsy sampling, 14.6 (± 5.7). min for TN biopsy sampling and 2.2 (± 0.5) min for cloacal incision suture. Besides biopsy sampling, most organs of clinical relevance for avian species were successfully viewed. In the second stage of the study, 12 posture pullets (224.2 ± 16.9g.) were randomly assigned into HN (n = 6) or TN (n = 6) group for liver biopsy. In all animals (n = 12) liver biopsies were successfully carried out. Total surgical time was 33.5 (± 13.7) minutes in the TN group and 32.3 (± 15.8) minutes in the HN group (p = 0.8940). Nine samples presented minimal artifacts and were classified as optimal for histological assessment. In conclusion, both NT and NH transcloacal approaches are feasible for intracoelomatic biopsy sampling in chicken, as an experimental model for exotic avian. |
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Notes total e híbrida em frangos (Gallus gallus domesticus) como modelo experimental para aves exóticasTotal and hybrid notes in cockerel (Gallus gallus domesticus) as an experimental model for exotic avianAvesEndoscopiaAnimais silvestresAnimais exóticosAbordagem intracloacalBirdsEndoscopyWildlifeExotic animalsIntracloacal approachCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAThe purpose of this study was to assess the feasibility of the intracloacal hybrid-NOTES (HN) or total-NOTES (TN) techniques in chickens (Gallus gallus domesticus) for biopsy sampling, in two experimental stages: (1) to assess the HN and TN techniques in chicken cadavers for liver and kidney biopsy, regarding the quality of access, complications and general findings; (2) to compare the HN or TN technique for liver biopsy in posture pullets regarding operative time, complications, technical difficulties and histological quality of liver samples. In the first stage of the study, four cadavers of broiler chicken, weighting 2.6 (± 0.7) kg. Both HN and TN surgical approaches for biopsy sampling were performed in all cadavers. Surgical steps were classified into: (1) surgical access, which involved cloacoscopy and endoscope entry into the celomatic cavity. This step was common to both treatments; (2) HN liver and renal biopsy sampling, by insertion of a 5-fr. biopsy forceps through a percutaneous 14G catheter sheath; (3) TN liver and renal biopsy sampling, by insertion of the biopsy forceps through the same cloacal access, in parallel to the endoscope; (4) cloacal synthesis. Both surgical approaches were successfully carried out in all cadavers (n = 4). Time for the surgical steps were 2.7 (± 1.4) min for surgical access, 9.2 (± 3.4) minutes for HN biopsy sampling, 14.6 (± 5.7). min for TN biopsy sampling and 2.2 (± 0.5) min for cloacal incision suture. Besides biopsy sampling, most organs of clinical relevance for avian species were successfully viewed. In the second stage of the study, 12 posture pullets (224.2 ± 16.9g.) were randomly assigned into HN (n = 6) or TN (n = 6) group for liver biopsy. In all animals (n = 12) liver biopsies were successfully carried out. Total surgical time was 33.5 (± 13.7) minutes in the TN group and 32.3 (± 15.8) minutes in the HN group (p = 0.8940). Nine samples presented minimal artifacts and were classified as optimal for histological assessment. In conclusion, both NT and NH transcloacal approaches are feasible for intracoelomatic biopsy sampling in chicken, as an experimental model for exotic avian.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESO objetivo do presente estudo foi analisar a factibilidade na técnica de NOTES Híbrida (NH) ou NOTES Total (NT) intracloacal, em frangos (Gallus gallus domesticus) para obtenção de amostras de biopsias, mediante: (1) realização de ambos tratamentos cirúrgicos em cadáveres de frango para biopsia hepática e renal e avaliar quanto a qualidade do acesso, complicações e achados durante a execução; (2) comparação da técnica de NH ou NT, em frangas de postura para biopsia hepática quanto a parâmetros de tempo operatório, complicações e dificuldades da técnica e qualidade na amostra do fígado. Na primeira etapa do estudo, quatro cadáveres de frango de corte (n=4), com peso médio de 2,6±0,7 kg. Foram realizadas ambas as abordagens cirúrgicas em todos os animais. As etapas cirúrgicas foram divididas em: (1) acesso cirúrgico, etapa da cloacoscopia até entrada do endoscópio de 1,9 mm com sua camisa de proteção com 3,3mm na cavidade celomática, etapa comum aos dois tratamentos; (2) coleta de biopsias por NH, mediante inserção de pinça de biopsia 1,7mm. através de um cateter 14G para obtenção de fragmentos hepáticos e renais; (3) coleta de biópsia por NT, mediante inserção da pinça de biópsia pelo mesmo acesso transcloacal, em paralelo com o endoscópio, e a obtenção de fragmentos hepáticos e renais; (4) síntese da incisão de acesso cloacal. Os tempos cirúrgicos das etapas intraoperatórias foram de 2,7±1,4 min. para o acesso cirúgico, 9,2±3,4 min. para realização das biópsias pelo tratamento NH, 14,6±5,7 min. para o tratamento NT e 2,2 (±0,5) min. para a cloacorrafia. Além das coletas, foi possível visibilizar órgãos intracelomáticos de interesse clínico. Na segunda etapa, foram utilizadas 12 frangas de postura (224,2±16,9g), distribuídas aleatoriamente em NH (n=6) e NT (n=6), para realização de biopsia hepática. Em todos os animais foi possível realizar a biopsia hepática, sendo que o tempo total para realização dos procedimentos cirúrgicos foi de 33,5±13,7 min. no NT e 32,3±15,8 min no NH (p=0,8940). Nove amostras apresentaram avaliação com mínimo afetado e foram consideradas de excelente qualidade para avaliação histológica. Concluiu-se que as técnicas de NT e NH são factíveis para coleta de biopsia em galináceos, como um modelo experimental para aves exóticas.Universidade Federal de Santa MariaBrasilMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaCentro de Ciências RuraisSilva, Marco Augusto Machadohttp://lattes.cnpq.br/2134206254307043Brun, Maurício VelosoCorrêa, Luís Felipe DutraTeixeira, Pedro Paulo MaiaPedrotti, Luís Fernando2021-07-01T12:48:35Z2021-07-01T12:48:35Z2020-02-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/21284porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2021-07-02T06:02:06Zoai:repositorio.ufsm.br:1/21284Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2021-07-02T06:02:06Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Notes total e híbrida em frangos (Gallus gallus domesticus) como modelo experimental para aves exóticas Total and hybrid notes in cockerel (Gallus gallus domesticus) as an experimental model for exotic avian |
title |
Notes total e híbrida em frangos (Gallus gallus domesticus) como modelo experimental para aves exóticas |
spellingShingle |
Notes total e híbrida em frangos (Gallus gallus domesticus) como modelo experimental para aves exóticas Pedrotti, Luís Fernando Aves Endoscopia Animais silvestres Animais exóticos Abordagem intracloacal Birds Endoscopy Wildlife Exotic animals Intracloacal approach CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Notes total e híbrida em frangos (Gallus gallus domesticus) como modelo experimental para aves exóticas |
title_full |
Notes total e híbrida em frangos (Gallus gallus domesticus) como modelo experimental para aves exóticas |
title_fullStr |
Notes total e híbrida em frangos (Gallus gallus domesticus) como modelo experimental para aves exóticas |
title_full_unstemmed |
Notes total e híbrida em frangos (Gallus gallus domesticus) como modelo experimental para aves exóticas |
title_sort |
Notes total e híbrida em frangos (Gallus gallus domesticus) como modelo experimental para aves exóticas |
author |
Pedrotti, Luís Fernando |
author_facet |
Pedrotti, Luís Fernando |
author_role |
author |
dc.contributor.none.fl_str_mv |
Silva, Marco Augusto Machado http://lattes.cnpq.br/2134206254307043 Brun, Maurício Veloso Corrêa, Luís Felipe Dutra Teixeira, Pedro Paulo Maia |
dc.contributor.author.fl_str_mv |
Pedrotti, Luís Fernando |
dc.subject.por.fl_str_mv |
Aves Endoscopia Animais silvestres Animais exóticos Abordagem intracloacal Birds Endoscopy Wildlife Exotic animals Intracloacal approach CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Aves Endoscopia Animais silvestres Animais exóticos Abordagem intracloacal Birds Endoscopy Wildlife Exotic animals Intracloacal approach CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The purpose of this study was to assess the feasibility of the intracloacal hybrid-NOTES (HN) or total-NOTES (TN) techniques in chickens (Gallus gallus domesticus) for biopsy sampling, in two experimental stages: (1) to assess the HN and TN techniques in chicken cadavers for liver and kidney biopsy, regarding the quality of access, complications and general findings; (2) to compare the HN or TN technique for liver biopsy in posture pullets regarding operative time, complications, technical difficulties and histological quality of liver samples. In the first stage of the study, four cadavers of broiler chicken, weighting 2.6 (± 0.7) kg. Both HN and TN surgical approaches for biopsy sampling were performed in all cadavers. Surgical steps were classified into: (1) surgical access, which involved cloacoscopy and endoscope entry into the celomatic cavity. This step was common to both treatments; (2) HN liver and renal biopsy sampling, by insertion of a 5-fr. biopsy forceps through a percutaneous 14G catheter sheath; (3) TN liver and renal biopsy sampling, by insertion of the biopsy forceps through the same cloacal access, in parallel to the endoscope; (4) cloacal synthesis. Both surgical approaches were successfully carried out in all cadavers (n = 4). Time for the surgical steps were 2.7 (± 1.4) min for surgical access, 9.2 (± 3.4) minutes for HN biopsy sampling, 14.6 (± 5.7). min for TN biopsy sampling and 2.2 (± 0.5) min for cloacal incision suture. Besides biopsy sampling, most organs of clinical relevance for avian species were successfully viewed. In the second stage of the study, 12 posture pullets (224.2 ± 16.9g.) were randomly assigned into HN (n = 6) or TN (n = 6) group for liver biopsy. In all animals (n = 12) liver biopsies were successfully carried out. Total surgical time was 33.5 (± 13.7) minutes in the TN group and 32.3 (± 15.8) minutes in the HN group (p = 0.8940). Nine samples presented minimal artifacts and were classified as optimal for histological assessment. In conclusion, both NT and NH transcloacal approaches are feasible for intracoelomatic biopsy sampling in chicken, as an experimental model for exotic avian. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02-27 2021-07-01T12:48:35Z 2021-07-01T12:48:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/21284 |
url |
http://repositorio.ufsm.br/handle/1/21284 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária Centro de Ciências Rurais |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922069567766528 |