Infecção latente em coelhos inoculados com o herpesvírus bovino tipo 5 (BHV-5): reativação, excreção viral e recrudescência da enfermidade neurológica
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/26987 |
Resumo: | Weanling rabbits are highly susceptible to bovine herpesvirus type-5 (BHV-5) and after experimental infection often develop an acute fatal neurological disease. To determine whether acute infection is followed by the establishment of latent infection, older rabbits (3-4 and 5-6-months-old), which are less susceptible to acute neurological infection, were inoculated by the intranasal or conjunctival routes with two south american BHV-5 isolates (613 and EVI-88). Eight rabbits (8/27) incoculated with isolate 613 and one (1/34) inoculated with isolate EVI-88 developed neurological disease and died during acute infection a few days after inoculation. In addition, three rabbits developed neurological disease and died 34, 41 and 58 days after virus inoculation. Fifty six to 62 days after inoculation, the remaining rabbits were submitted to dexamethasone (Dx) administration to reactivate the infection. Twenty five out of 44 rabbits (56.8%) shed virus in nasal or ocular secretions after Dx treatment. The frequency and course of virus shedding varied with the virus isolate, route of inoculation and Dx dose. Virus shedding was first detected at day two post-Dx treatment (dpDx) and lasted from one to eleven days (average 2.8 days). The frequency of virus reactivation and shedding was higher among rabbits inoculated with isolate 613 (12/16 or 75%) than among rabbits inoculated with isolate EVI-88 (13/28 or 46.4%). Virus reactivation upon Dx administration was accompanied by neurological signs in nine rabbits (20.4%), resulting in six deaths (13.6%). The neurological signs resembled those observed during acute infection; started as early as at day 3 pDx and lasted from less than 24 hours to twelve days. In addition, three rabbits showed signs of neurological infection followed by death 31 to 54 days after Dx treatment. Infectious virus, viral nucleic acids and inflammatory changes were detected in the brain of these rabbits. The late onset of clinical disease after acute infection or Dx administration some rabbits suggests these animals experienced a spontaneous or a delayed-onset Dx-induced viral reactivation and recrudescence of neurological disease. These results demonstrate that BHV-5 does establish a spontaneously and chemically reactivatable latent infection in rabbits after acute infection. Reactivation of latent infection courses with virus shedding and frequently with recrudescence of neurological disease. Further studies in rabbits may help understanding the neuropathogenesis of BHV-5 latent infection in cattle. |
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Infecção latente em coelhos inoculados com o herpesvírus bovino tipo 5 (BHV-5): reativação, excreção viral e recrudescência da enfermidade neurológicaLatent infection by bovine herpesvirus type-5 (BHV-5) in experimentally infected rabbits: virus reactivation, shedding and recrudescence of neurological diseaseHerpesvírus bovino tipo 5BHV-5Infecção latenteCoelhosBovine herpesvirus type 5Latent infectionRabbitsCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAWeanling rabbits are highly susceptible to bovine herpesvirus type-5 (BHV-5) and after experimental infection often develop an acute fatal neurological disease. To determine whether acute infection is followed by the establishment of latent infection, older rabbits (3-4 and 5-6-months-old), which are less susceptible to acute neurological infection, were inoculated by the intranasal or conjunctival routes with two south american BHV-5 isolates (613 and EVI-88). Eight rabbits (8/27) incoculated with isolate 613 and one (1/34) inoculated with isolate EVI-88 developed neurological disease and died during acute infection a few days after inoculation. In addition, three rabbits developed neurological disease and died 34, 41 and 58 days after virus inoculation. Fifty six to 62 days after inoculation, the remaining rabbits were submitted to dexamethasone (Dx) administration to reactivate the infection. Twenty five out of 44 rabbits (56.8%) shed virus in nasal or ocular secretions after Dx treatment. The frequency and course of virus shedding varied with the virus isolate, route of inoculation and Dx dose. Virus shedding was first detected at day two post-Dx treatment (dpDx) and lasted from one to eleven days (average 2.8 days). The frequency of virus reactivation and shedding was higher among rabbits inoculated with isolate 613 (12/16 or 75%) than among rabbits inoculated with isolate EVI-88 (13/28 or 46.4%). Virus reactivation upon Dx administration was accompanied by neurological signs in nine rabbits (20.4%), resulting in six deaths (13.6%). The neurological signs resembled those observed during acute infection; started as early as at day 3 pDx and lasted from less than 24 hours to twelve days. In addition, three rabbits showed signs of neurological infection followed by death 31 to 54 days after Dx treatment. Infectious virus, viral nucleic acids and inflammatory changes were detected in the brain of these rabbits. The late onset of clinical disease after acute infection or Dx administration some rabbits suggests these animals experienced a spontaneous or a delayed-onset Dx-induced viral reactivation and recrudescence of neurological disease. These results demonstrate that BHV-5 does establish a spontaneously and chemically reactivatable latent infection in rabbits after acute infection. Reactivation of latent infection courses with virus shedding and frequently with recrudescence of neurological disease. Further studies in rabbits may help understanding the neuropathogenesis of BHV-5 latent infection in cattle.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESCoelhos recém desmamados são altamente susceptíveis à infecção pelo herpesvírus bovino tipo 5 (BHV-5) e desenvolvem uma doença neurológica aguda fatal após inoculação experimental. Para determinar se a infecção aguda é seguida do estabelecimento de infecção latente, coelhos adultos (3-4 e 5-6 meses), que são menos susceptíveis à infecção neurológica aguda, foram inoculados pela via intranasal ou conjuntival com duas amostras sul americanas do BHV-5 (613 e EVI-88). Oito coelhos (8/27) inoculados com o isolado 613 e um coelho (1/34) inoculado com o isolado EVI-88, desenvolveram doença neurológica e morreram durante a infecção aguda, poucos dias após a inoculação. Três outros coelhos desenvolveram doença neurológica e morreram nos dias 34, 41 e 58 após a inoculação. Aos 56 e 62 dpi, os coelhos remanescentes foram submetidos à administração de dexametasona (Dx) para reativação da infecção latente. Vinte e cinco de 44 coelhos (56,8%) excretaram o vírus em secreções nasais ou conjuntivais após a administração de Dx. A freqüência e o curso da excreção viral variaram com o isolado, via de inoculação e dose de Dx. A excreção foi detectada a partir do segundo dia pós-inoculação (dpDx) e durou de dois a onze dias (média 2,8 dias). A freqüência de reativação e excreção viral foi maior entre os coelhos inoculados com a amostra 613 (12/16 ou 75%) do que entre os coelhos inoculados com o isolado EVI-88 (13/28 ou 46,4%). A reativação viral após a administração com Dx foi acompanhada de sinais neurológicos em nove coelhos (20,4%), resultando em seis mortes (13,6%). Os sinais neurológicos foram semelhantes aos observados durante a infecção aguda; iniciaram no dia 3 pDx e duraram de 24 horas a doze dias. Adicionalmente, três coelhos apresentaram sinais neurológicos seguidos de morte nos dias 31 e 54 pDx. Vírus, ácidos nucléicos virais e alterações inflamatórias foram detectados no cérebro desses animais. A manifestação tardia dos sinais clínicos em alguns coelhos sugere que esses animais apresentaram reativação espontânea ou reativação viral e recrudescência tardia da doença neurológica induzida pela Dx. Esses resultados demonstram que o BHV-5 estabelece infecção latente após a infecção aguda em coelhos e que a infecção pode ser reativada espontaneamente ou induzida por corticosteróides. A reativação da infecção cursa com excreção viral e freqüentemente com recrudescência da doença neurológica. Estudos adicionais em coelhos podem contribuir para o conhecimento da neuropatogenia da infecção latente pelo BHV-5 em bovinos.Universidade Federal de Santa MariaBrasilMedicina VeterináriaUFSMPrograma de Pós-Graduação em Medicina VeterináriaCentro de Ciências RuraisWeiblen, Rudihttp://lattes.cnpq.br/7946350215388090Flores, Eduardo FurtadoZanella, Janice R. CiacciCaron, Luizinho2022-11-17T19:43:47Z2022-11-17T19:43:47Z2001-02-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/26987porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-11-17T19:43:47Zoai:repositorio.ufsm.br:1/26987Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-11-17T19:43:47Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Infecção latente em coelhos inoculados com o herpesvírus bovino tipo 5 (BHV-5): reativação, excreção viral e recrudescência da enfermidade neurológica Latent infection by bovine herpesvirus type-5 (BHV-5) in experimentally infected rabbits: virus reactivation, shedding and recrudescence of neurological disease |
title |
Infecção latente em coelhos inoculados com o herpesvírus bovino tipo 5 (BHV-5): reativação, excreção viral e recrudescência da enfermidade neurológica |
spellingShingle |
Infecção latente em coelhos inoculados com o herpesvírus bovino tipo 5 (BHV-5): reativação, excreção viral e recrudescência da enfermidade neurológica Caron, Luizinho Herpesvírus bovino tipo 5 BHV-5 Infecção latente Coelhos Bovine herpesvirus type 5 Latent infection Rabbits CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Infecção latente em coelhos inoculados com o herpesvírus bovino tipo 5 (BHV-5): reativação, excreção viral e recrudescência da enfermidade neurológica |
title_full |
Infecção latente em coelhos inoculados com o herpesvírus bovino tipo 5 (BHV-5): reativação, excreção viral e recrudescência da enfermidade neurológica |
title_fullStr |
Infecção latente em coelhos inoculados com o herpesvírus bovino tipo 5 (BHV-5): reativação, excreção viral e recrudescência da enfermidade neurológica |
title_full_unstemmed |
Infecção latente em coelhos inoculados com o herpesvírus bovino tipo 5 (BHV-5): reativação, excreção viral e recrudescência da enfermidade neurológica |
title_sort |
Infecção latente em coelhos inoculados com o herpesvírus bovino tipo 5 (BHV-5): reativação, excreção viral e recrudescência da enfermidade neurológica |
author |
Caron, Luizinho |
author_facet |
Caron, Luizinho |
author_role |
author |
dc.contributor.none.fl_str_mv |
Weiblen, Rudi http://lattes.cnpq.br/7946350215388090 Flores, Eduardo Furtado Zanella, Janice R. Ciacci |
dc.contributor.author.fl_str_mv |
Caron, Luizinho |
dc.subject.por.fl_str_mv |
Herpesvírus bovino tipo 5 BHV-5 Infecção latente Coelhos Bovine herpesvirus type 5 Latent infection Rabbits CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
topic |
Herpesvírus bovino tipo 5 BHV-5 Infecção latente Coelhos Bovine herpesvirus type 5 Latent infection Rabbits CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Weanling rabbits are highly susceptible to bovine herpesvirus type-5 (BHV-5) and after experimental infection often develop an acute fatal neurological disease. To determine whether acute infection is followed by the establishment of latent infection, older rabbits (3-4 and 5-6-months-old), which are less susceptible to acute neurological infection, were inoculated by the intranasal or conjunctival routes with two south american BHV-5 isolates (613 and EVI-88). Eight rabbits (8/27) incoculated with isolate 613 and one (1/34) inoculated with isolate EVI-88 developed neurological disease and died during acute infection a few days after inoculation. In addition, three rabbits developed neurological disease and died 34, 41 and 58 days after virus inoculation. Fifty six to 62 days after inoculation, the remaining rabbits were submitted to dexamethasone (Dx) administration to reactivate the infection. Twenty five out of 44 rabbits (56.8%) shed virus in nasal or ocular secretions after Dx treatment. The frequency and course of virus shedding varied with the virus isolate, route of inoculation and Dx dose. Virus shedding was first detected at day two post-Dx treatment (dpDx) and lasted from one to eleven days (average 2.8 days). The frequency of virus reactivation and shedding was higher among rabbits inoculated with isolate 613 (12/16 or 75%) than among rabbits inoculated with isolate EVI-88 (13/28 or 46.4%). Virus reactivation upon Dx administration was accompanied by neurological signs in nine rabbits (20.4%), resulting in six deaths (13.6%). The neurological signs resembled those observed during acute infection; started as early as at day 3 pDx and lasted from less than 24 hours to twelve days. In addition, three rabbits showed signs of neurological infection followed by death 31 to 54 days after Dx treatment. Infectious virus, viral nucleic acids and inflammatory changes were detected in the brain of these rabbits. The late onset of clinical disease after acute infection or Dx administration some rabbits suggests these animals experienced a spontaneous or a delayed-onset Dx-induced viral reactivation and recrudescence of neurological disease. These results demonstrate that BHV-5 does establish a spontaneously and chemically reactivatable latent infection in rabbits after acute infection. Reactivation of latent infection courses with virus shedding and frequently with recrudescence of neurological disease. Further studies in rabbits may help understanding the neuropathogenesis of BHV-5 latent infection in cattle. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-02-19 2022-11-17T19:43:47Z 2022-11-17T19:43:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/26987 |
url |
http://repositorio.ufsm.br/handle/1/26987 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária Centro de Ciências Rurais |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Medicina Veterinária UFSM Programa de Pós-Graduação em Medicina Veterinária Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1805922110972887040 |