Efeito do guaraná (Paullinia cupana) em parâmetros comportamentais e sistemas purinérgico e colinérgico em modelo de hiperlipidemia
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/18974 |
Resumo: | Hyperlipidemia is a condition associated with cognitive and inflammatory disorders, with has a high impact on cardiovascular diseases and central nervous system. Guarana, on the other hand, presents potent anti-inflammatory action and is able to act in the improvement of cogni tion. Thus, this study aimed to evaluate the effect of guarana on behavioral parameters, in purinergic and cholinergic systems of rats with hyperlipidemia. For this, we used adult male Wistar rats previously treated by gavage with guarana 12.5, 25 and 50 mg/kg/day or caffeine (0.2 mg/kg/day) for a period of 30 days. Hyperlipidemia was acutely induced by a single intraperitoneal injection with 500 mg/kg of Poloxamer-407. Simvastatin (0.04 mg/kg) was administered only after induction. Memory tests and anxiety evaluations were performed. The cortex, cerebellum, hippocampus, hypothalamus, and striatum were separated for evaluation of acetylcholinesterase activity. Peripheral blood lymphocytes were separated for the determination of the activity of purinergic enzymes. In addition, serum concentrations of nucleotides and nucleosides, biochemical parameters and inflammatory profile were determined. Our data demonstrate that guarana powder and caffeine were able to prevent elevation of total cholesterol and LDL levels and in aspartate aminotransferase activity caused by hyperlipidemia. These compounds acted to prevent alterations in AChE activity and decreased memory capacity caused by hyperlipidemia in hippocampus. Guarana and caffeine promoted changes in the activity of E-NTPDase and E-ADA enzymes as a compensatory organic response, contributing to the change in inflammatory profile. Guarana and caffeine also acted to prevent elevation of ATP levels and increase extracellular adenosine. In addition, they acted to prevent the increase of INF-γ and potentiated the elevation of IL-4 levels caused by hyperlipidemia, contributing to an anti -inflammatory profile. These results obtained suggest that guarana is a promising compound to be used as an adjuvant therapy to benefit patients with hyperlipidemia and cognitive disorders. |
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Efeito do guaraná (Paullinia cupana) em parâmetros comportamentais e sistemas purinérgico e colinérgico em modelo de hiperlipidemiaEffect of guarana (Paullinia cupana) on behavioral parameters and purinergic and cholinergic systems in hyperlipidemia modelHiperlipidemiaMemóriaPaullinia cupanaSistema colinérgicoSistema purinérgicoCholinergic systemHyperlipidemiaMemoryPurinergic systemCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAHyperlipidemia is a condition associated with cognitive and inflammatory disorders, with has a high impact on cardiovascular diseases and central nervous system. Guarana, on the other hand, presents potent anti-inflammatory action and is able to act in the improvement of cogni tion. Thus, this study aimed to evaluate the effect of guarana on behavioral parameters, in purinergic and cholinergic systems of rats with hyperlipidemia. For this, we used adult male Wistar rats previously treated by gavage with guarana 12.5, 25 and 50 mg/kg/day or caffeine (0.2 mg/kg/day) for a period of 30 days. Hyperlipidemia was acutely induced by a single intraperitoneal injection with 500 mg/kg of Poloxamer-407. Simvastatin (0.04 mg/kg) was administered only after induction. Memory tests and anxiety evaluations were performed. The cortex, cerebellum, hippocampus, hypothalamus, and striatum were separated for evaluation of acetylcholinesterase activity. Peripheral blood lymphocytes were separated for the determination of the activity of purinergic enzymes. In addition, serum concentrations of nucleotides and nucleosides, biochemical parameters and inflammatory profile were determined. Our data demonstrate that guarana powder and caffeine were able to prevent elevation of total cholesterol and LDL levels and in aspartate aminotransferase activity caused by hyperlipidemia. These compounds acted to prevent alterations in AChE activity and decreased memory capacity caused by hyperlipidemia in hippocampus. Guarana and caffeine promoted changes in the activity of E-NTPDase and E-ADA enzymes as a compensatory organic response, contributing to the change in inflammatory profile. Guarana and caffeine also acted to prevent elevation of ATP levels and increase extracellular adenosine. In addition, they acted to prevent the increase of INF-γ and potentiated the elevation of IL-4 levels caused by hyperlipidemia, contributing to an anti -inflammatory profile. These results obtained suggest that guarana is a promising compound to be used as an adjuvant therapy to benefit patients with hyperlipidemia and cognitive disorders.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESA hiperlipidemia é uma condição associada a distúrbios cognitivos e inflamatórios, repercutindo em um alto impacto nas doenças cardiovasculares e no sistema nervoso central. Já o guaraná (Paullinia cupana), por sua vez, apresenta potente ação anti-inflamatória e é capaz de atuar na melhora da cognição. Deste modo, este trabalho teve como objetivo avaliar o efeito do guaraná nos parâmetros comportamentais e nos sistemas purinérgico e colinérgico de ratos submetidos à modelo de hiperlipidemia. Para isso, foram utilizados ratos machos Wistar adultos tratados previamente, por gavagem, com guaraná em pó diluído nas doses de 12,5, 25 e 50 mg/kg/dia e cafeína (0,2 mg/kg/dia), por um período de 30 dias. A hiperlipidemia foi induzida, de forma aguda, através de uma única injeção via intraperitoneal com 500 mg/kg de Poloxamer-407. A sinvastatina (0,04 mg/kg), foi administrada somente após a indução. Foram realizados testes de memória e avaliação da ansiedade. O córtex, cerebelo, hipocampo, hipotálamo e estriado foram separados para avaliação da atividade da acetilcolinesterase. Os linfócitos do sangue periférico foram separados para a determinação da atividade de enzimas purinérgicas. Além disso, foram determinadas as concentrações séricas dos nucleotídeos e nucleosídeos, parâmetros bioquímicos e perfil inflamatório. Nossos dados demonstram que o pó do guaraná e a cafeína foram capazes de evitar a elevação dos níveis de colesterol total, LDL e da atividade da aspartato aminotransferase, causados pela hiperlipidemia. Esses compostos atuaram na prevenção das alterações na atividade da AChE e na diminuição da capacidade de memória provocada pela hiperlipidemia no hipocampo. O guaraná e a cafeína promoveram alterações na atividade das enzimas E-NTPDase e E-ADA, como uma resposta orgânica compensatória, contribuindo assim, para a mudança no perfil inflamatório. O guaraná e a cafeína também atuaram prevenindo a elevação dos níveis de ATP e aumentando a adenosina extracelular. Além disso, agiram prevenindo o aumento do INF-γ e potencializando a elevação dos níveis de IL-4 provocado pela hiperlipidemia, contribuindo assim, para um perfil anti-inflamatório. Os dados obtidos sugerem que o guaraná é um composto promissor a ser usado como uma terapia adjuvante para beneficiar pacientes com hiperlipidemia e distúrbios cognitivos.Universidade Federal de Santa MariaBrasilBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaCentro de Ciências Naturais e ExatasLeal, Daniela Bitencourt Rosahttp://lattes.cnpq.br/3639683273462361Jaques, Jeandre Augusto dos Santoshttp://lattes.cnpq.br/9733439439501163Pereira, Patríciahttp://lattes.cnpq.br/1141660061525473Fachinetto, Roseleihttp://lattes.cnpq.br/7203076675431306Spanevello, Roselia Mariahttp://lattes.cnpq.br/3446031341157893Ruchel, Jader Betsch2019-11-19T19:27:06Z2019-11-19T19:27:06Z2017-08-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/18974porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2019-11-20T06:02:24Zoai:repositorio.ufsm.br:1/18974Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2019-11-20T06:02:24Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Efeito do guaraná (Paullinia cupana) em parâmetros comportamentais e sistemas purinérgico e colinérgico em modelo de hiperlipidemia Effect of guarana (Paullinia cupana) on behavioral parameters and purinergic and cholinergic systems in hyperlipidemia model |
title |
Efeito do guaraná (Paullinia cupana) em parâmetros comportamentais e sistemas purinérgico e colinérgico em modelo de hiperlipidemia |
spellingShingle |
Efeito do guaraná (Paullinia cupana) em parâmetros comportamentais e sistemas purinérgico e colinérgico em modelo de hiperlipidemia Ruchel, Jader Betsch Hiperlipidemia Memória Paullinia cupana Sistema colinérgico Sistema purinérgico Cholinergic system Hyperlipidemia Memory Purinergic system CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeito do guaraná (Paullinia cupana) em parâmetros comportamentais e sistemas purinérgico e colinérgico em modelo de hiperlipidemia |
title_full |
Efeito do guaraná (Paullinia cupana) em parâmetros comportamentais e sistemas purinérgico e colinérgico em modelo de hiperlipidemia |
title_fullStr |
Efeito do guaraná (Paullinia cupana) em parâmetros comportamentais e sistemas purinérgico e colinérgico em modelo de hiperlipidemia |
title_full_unstemmed |
Efeito do guaraná (Paullinia cupana) em parâmetros comportamentais e sistemas purinérgico e colinérgico em modelo de hiperlipidemia |
title_sort |
Efeito do guaraná (Paullinia cupana) em parâmetros comportamentais e sistemas purinérgico e colinérgico em modelo de hiperlipidemia |
author |
Ruchel, Jader Betsch |
author_facet |
Ruchel, Jader Betsch |
author_role |
author |
dc.contributor.none.fl_str_mv |
Leal, Daniela Bitencourt Rosa http://lattes.cnpq.br/3639683273462361 Jaques, Jeandre Augusto dos Santos http://lattes.cnpq.br/9733439439501163 Pereira, Patrícia http://lattes.cnpq.br/1141660061525473 Fachinetto, Roselei http://lattes.cnpq.br/7203076675431306 Spanevello, Roselia Maria http://lattes.cnpq.br/3446031341157893 |
dc.contributor.author.fl_str_mv |
Ruchel, Jader Betsch |
dc.subject.por.fl_str_mv |
Hiperlipidemia Memória Paullinia cupana Sistema colinérgico Sistema purinérgico Cholinergic system Hyperlipidemia Memory Purinergic system CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
topic |
Hiperlipidemia Memória Paullinia cupana Sistema colinérgico Sistema purinérgico Cholinergic system Hyperlipidemia Memory Purinergic system CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Hyperlipidemia is a condition associated with cognitive and inflammatory disorders, with has a high impact on cardiovascular diseases and central nervous system. Guarana, on the other hand, presents potent anti-inflammatory action and is able to act in the improvement of cogni tion. Thus, this study aimed to evaluate the effect of guarana on behavioral parameters, in purinergic and cholinergic systems of rats with hyperlipidemia. For this, we used adult male Wistar rats previously treated by gavage with guarana 12.5, 25 and 50 mg/kg/day or caffeine (0.2 mg/kg/day) for a period of 30 days. Hyperlipidemia was acutely induced by a single intraperitoneal injection with 500 mg/kg of Poloxamer-407. Simvastatin (0.04 mg/kg) was administered only after induction. Memory tests and anxiety evaluations were performed. The cortex, cerebellum, hippocampus, hypothalamus, and striatum were separated for evaluation of acetylcholinesterase activity. Peripheral blood lymphocytes were separated for the determination of the activity of purinergic enzymes. In addition, serum concentrations of nucleotides and nucleosides, biochemical parameters and inflammatory profile were determined. Our data demonstrate that guarana powder and caffeine were able to prevent elevation of total cholesterol and LDL levels and in aspartate aminotransferase activity caused by hyperlipidemia. These compounds acted to prevent alterations in AChE activity and decreased memory capacity caused by hyperlipidemia in hippocampus. Guarana and caffeine promoted changes in the activity of E-NTPDase and E-ADA enzymes as a compensatory organic response, contributing to the change in inflammatory profile. Guarana and caffeine also acted to prevent elevation of ATP levels and increase extracellular adenosine. In addition, they acted to prevent the increase of INF-γ and potentiated the elevation of IL-4 levels caused by hyperlipidemia, contributing to an anti -inflammatory profile. These results obtained suggest that guarana is a promising compound to be used as an adjuvant therapy to benefit patients with hyperlipidemia and cognitive disorders. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-28 2019-11-19T19:27:06Z 2019-11-19T19:27:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/18974 |
url |
http://repositorio.ufsm.br/handle/1/18974 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica Centro de Ciências Naturais e Exatas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922091478810624 |