Avaliação anatomopatológica de fígado, rim, pulmão e cérebro de camundongos expostos ao composto ditelureto de difenila

Detalhes bibliográficos
Autor(a) principal: Luz, Sônia Cristina Almeida da
Data de Publicação: 2014
Tipo de documento: Tese
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000c39h
Texto Completo: http://repositorio.ufsm.br/handle/1/4480
Resumo: Diphenyl ditelluride (PhTe)2 is an organic tellurium compound widely used as an intermediate in organic synthesis reactions and in the industry. Several studies have shown that this compound causes various toxic effects in different tissues. Particularly notable are teratogenic, genotoxic and neurotoxic effects. Although the mechanisms involved in cytotoxicity mediated by the compound are not fully understood, its pro-oxidant activity is directly related to its toxicology. Inhibition of sulfhydryl enzyme and antioxidant, thiol depletion, and lipid peroxidation are biochemical changes found in tissues such as blood, brain, liver and kidney of animals exposed to the compound. On the other hand, there are few histopathological studies showing the effects of exposure to (PhTe)2 in specific organs. Thus, the present study aimed to carry out the anatomopathological evaluation of the brain, kidney, liver and lung of mice exposed subcutaneously acutely (1 dose of 250 μmol/kg) and subchronically (doses 10 and 50 μmol/kg for 7 and 14 days) to the compound. Histological analysis showed that the hepatocytes of animals exposed subchronically to both doses of the compound developed extensive vacuolization of the cytoplasm or hydropic degeneration and hyperchromatic nuclei. Subchronic exposure to 50μmol/kg has also induced nonspecific focal hepatitis and focal necrosis. Hepatic steatosis was observed in animals acutely exposed to the compound. In the kidney, acute and/or subchronic intoxication with (PhTe)2 caused vacuolar degeneration or hydropic degeneration, atrophy and hypertrophy of renal tubules, forming hyaline casts and acute tubular necrosis. Pulmonar edema and vascular congestion were found in the lungs of animals exposed to (PhTe)2 50μmol/kg. Histological analysis of brain tissue revealed that acute and subchronic exposure to (PhTe)2 induced neurodegenerative disorders (neuropil vacuolation, status spongiosus) in the hippocampus and thalamus. The presence of neuronal nuclear pyknosis, the loss of neuronal viability and neuronal death were observed particularly in the hippocampus. Additionally, through immunohistochemistry, we identified glial reaction and loss of pre-synaptic activity in the thalamus and cortex, corresponding to areas of spongiform change. Our findings suggest that exposure to (PhTe)2, subcutaneously causes severe morphological changes in liver, kidney, lung and brain. The data obtained will contribute significantly to increase the knowledge on the toxicology of the compound (PhTe)2, since this is the first work that evaluates the histology of tissues of various organs after intoxication with the compound.
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spelling Avaliação anatomopatológica de fígado, rim, pulmão e cérebro de camundongos expostos ao composto ditelureto de difenilaAnatomopathological evaluation of liver, kidney, lung and brain of mice exposed to diphenyl ditellurideDitelureto de difenilaHistologiaFígadoRimPulmãoCérebroDiphenyl ditellurideHistologyLiverKidneyLungBrainCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICADiphenyl ditelluride (PhTe)2 is an organic tellurium compound widely used as an intermediate in organic synthesis reactions and in the industry. Several studies have shown that this compound causes various toxic effects in different tissues. Particularly notable are teratogenic, genotoxic and neurotoxic effects. Although the mechanisms involved in cytotoxicity mediated by the compound are not fully understood, its pro-oxidant activity is directly related to its toxicology. Inhibition of sulfhydryl enzyme and antioxidant, thiol depletion, and lipid peroxidation are biochemical changes found in tissues such as blood, brain, liver and kidney of animals exposed to the compound. On the other hand, there are few histopathological studies showing the effects of exposure to (PhTe)2 in specific organs. Thus, the present study aimed to carry out the anatomopathological evaluation of the brain, kidney, liver and lung of mice exposed subcutaneously acutely (1 dose of 250 μmol/kg) and subchronically (doses 10 and 50 μmol/kg for 7 and 14 days) to the compound. Histological analysis showed that the hepatocytes of animals exposed subchronically to both doses of the compound developed extensive vacuolization of the cytoplasm or hydropic degeneration and hyperchromatic nuclei. Subchronic exposure to 50μmol/kg has also induced nonspecific focal hepatitis and focal necrosis. Hepatic steatosis was observed in animals acutely exposed to the compound. In the kidney, acute and/or subchronic intoxication with (PhTe)2 caused vacuolar degeneration or hydropic degeneration, atrophy and hypertrophy of renal tubules, forming hyaline casts and acute tubular necrosis. Pulmonar edema and vascular congestion were found in the lungs of animals exposed to (PhTe)2 50μmol/kg. Histological analysis of brain tissue revealed that acute and subchronic exposure to (PhTe)2 induced neurodegenerative disorders (neuropil vacuolation, status spongiosus) in the hippocampus and thalamus. The presence of neuronal nuclear pyknosis, the loss of neuronal viability and neuronal death were observed particularly in the hippocampus. Additionally, through immunohistochemistry, we identified glial reaction and loss of pre-synaptic activity in the thalamus and cortex, corresponding to areas of spongiform change. Our findings suggest that exposure to (PhTe)2, subcutaneously causes severe morphological changes in liver, kidney, lung and brain. The data obtained will contribute significantly to increase the knowledge on the toxicology of the compound (PhTe)2, since this is the first work that evaluates the histology of tissues of various organs after intoxication with the compound.O ditelureto de difenila (PhTe)2 é um composto orgânico de telúrio amplamente usado na indústria, como na produção industrial de borracha, aço e vidro, indústria de nanopartículas, painéis solares, semicondutores bem como intermediário em reações de síntese orgânica. Vários estudos têm demonstrado que esse composto causa diversos efeitos tóxicos em diferentes tecidos. Dentre esses, destacam-se os teratogênicos, os genotóxicos e os neurotóxicos. Embora os mecanismos de ação envolvidos na citotoxicidade mediada pelo composto não se encontrem totalmente esclarecidos, sua atividade pró-oxidante está diretamente ligada a sua toxicologia. Inibição de enzimas sulfidrílicas e antioxidantes, depleção de tióis e peroxidação lipídica são alterações bioquímicas encontradas em tecidos como sangue, cérebro, fígado e rim de animais expostos ao composto. Por outro lado, são raros na literatura estudos histopatológicos mostrando os efeitos da exposição ao (PhTe)2 em órgãos específicos. Assim, o presente estudo teve como objetivo realizar a avaliação anatomopatológica de cérebro, rim, fígado e pulmão de camundongos expostos, via subcutânea, de forma aguda (1 dose de 250 μmol/kg) e subcrônica (doses de 10 e 50 μmol/kg por 7 e 14 dias) ao composto. As análises histológicas revelaram que os hepatócitos dos animais expostos subcronicamente a ambas as doses do composto desenvolveram extensa vacuolização do citoplasma ou degeneração hidrópica e núcleos hipercromáticos. A exposição subcrônica a 50 μmol/kg também induziu hepatite focal e não específica, e necrose focal. Esteatose hepática foi verificada nos animais agudamente expostos ao composto. Em rim, a intoxicação aguda e subcrônica com (PhTe)2 provocou degeneração vacuolar ou degeneração hidrópica, atrofia e hipertrofia de túbulos renais, formação de moldes (casts) hialinos e necrose tubular aguda. Edema e congestão vascular foram encontrados nos pulmões dos animais expostos a 50 μmol/kg de (PhTe)2. A análise histológica do tecido cerebral revelou que a exposição aguda e subcrônica ao (PhTe)2 induziu alterações neurodegenerativas (vacuolização do neurópilo, status spongiosus) na região do tálamo e hipocampo. A presença de picnose nuclear de neurônios, a perda de viabilidade neuronal e foram particularmente observados no na camada granular do hipocampo. Adicionalmente, através de técnica imunoistoquímica, foram identificadas reação glial e perda de atividade pré-sináptica na região do tálamo e córtex, correspondentes às áreas de alteração espongiforme. Nossos achados sugerem que a exposição ao (PhTe)2, por via subcutânea, provoca severas alterações morfológicas em fígado, rim, pulmão e cérebro. Os dados obtidos contribuirão de forma significativa para aumentar o conhecimento sobre a toxicologia do composto (PhTe)2, uma vez que, este é o primeiro trabalho que avalia a histologia de tecidos de vários órgãos após a intoxicação com o composto.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaBarbosa, Nilda Berenice de Vargashttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795127P7Rocha, João Batista Teixeira dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782281H2Leal, Daniela Bitencourt Rosahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7Rosemberg, Denis Broockhttp://lattes.cnpq.br/7713953979203056Pinton, Simonehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4282614T6Roos, Daniel Henriquehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4710781H2Luz, Sônia Cristina Almeida da2015-04-012015-04-012014-08-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfapplication/pdfLUZ, Sônia Cristina Almeida da. ANATOMOPATHOLOGICAL EVALUATION OF LIVER, KIDNEY, LUNG AND BRAIN OF MICE EXPOSED TO DIPHENYL DITELLURIDE. 2014. 98 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/4480ark:/26339/001300000c39hporinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2017-08-28T21:08:08Zoai:repositorio.ufsm.br:1/4480Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2017-08-28T21:08:08Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Avaliação anatomopatológica de fígado, rim, pulmão e cérebro de camundongos expostos ao composto ditelureto de difenila
Anatomopathological evaluation of liver, kidney, lung and brain of mice exposed to diphenyl ditelluride
title Avaliação anatomopatológica de fígado, rim, pulmão e cérebro de camundongos expostos ao composto ditelureto de difenila
spellingShingle Avaliação anatomopatológica de fígado, rim, pulmão e cérebro de camundongos expostos ao composto ditelureto de difenila
Luz, Sônia Cristina Almeida da
Ditelureto de difenila
Histologia
Fígado
Rim
Pulmão
Cérebro
Diphenyl ditelluride
Histology
Liver
Kidney
Lung
Brain
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Avaliação anatomopatológica de fígado, rim, pulmão e cérebro de camundongos expostos ao composto ditelureto de difenila
title_full Avaliação anatomopatológica de fígado, rim, pulmão e cérebro de camundongos expostos ao composto ditelureto de difenila
title_fullStr Avaliação anatomopatológica de fígado, rim, pulmão e cérebro de camundongos expostos ao composto ditelureto de difenila
title_full_unstemmed Avaliação anatomopatológica de fígado, rim, pulmão e cérebro de camundongos expostos ao composto ditelureto de difenila
title_sort Avaliação anatomopatológica de fígado, rim, pulmão e cérebro de camundongos expostos ao composto ditelureto de difenila
author Luz, Sônia Cristina Almeida da
author_facet Luz, Sônia Cristina Almeida da
author_role author
dc.contributor.none.fl_str_mv Barbosa, Nilda Berenice de Vargas
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795127P7
Rocha, João Batista Teixeira da
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782281H2
Leal, Daniela Bitencourt Rosa
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706487E7
Rosemberg, Denis Broock
http://lattes.cnpq.br/7713953979203056
Pinton, Simone
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4282614T6
Roos, Daniel Henrique
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4710781H2
dc.contributor.author.fl_str_mv Luz, Sônia Cristina Almeida da
dc.subject.por.fl_str_mv Ditelureto de difenila
Histologia
Fígado
Rim
Pulmão
Cérebro
Diphenyl ditelluride
Histology
Liver
Kidney
Lung
Brain
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
topic Ditelureto de difenila
Histologia
Fígado
Rim
Pulmão
Cérebro
Diphenyl ditelluride
Histology
Liver
Kidney
Lung
Brain
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description Diphenyl ditelluride (PhTe)2 is an organic tellurium compound widely used as an intermediate in organic synthesis reactions and in the industry. Several studies have shown that this compound causes various toxic effects in different tissues. Particularly notable are teratogenic, genotoxic and neurotoxic effects. Although the mechanisms involved in cytotoxicity mediated by the compound are not fully understood, its pro-oxidant activity is directly related to its toxicology. Inhibition of sulfhydryl enzyme and antioxidant, thiol depletion, and lipid peroxidation are biochemical changes found in tissues such as blood, brain, liver and kidney of animals exposed to the compound. On the other hand, there are few histopathological studies showing the effects of exposure to (PhTe)2 in specific organs. Thus, the present study aimed to carry out the anatomopathological evaluation of the brain, kidney, liver and lung of mice exposed subcutaneously acutely (1 dose of 250 μmol/kg) and subchronically (doses 10 and 50 μmol/kg for 7 and 14 days) to the compound. Histological analysis showed that the hepatocytes of animals exposed subchronically to both doses of the compound developed extensive vacuolization of the cytoplasm or hydropic degeneration and hyperchromatic nuclei. Subchronic exposure to 50μmol/kg has also induced nonspecific focal hepatitis and focal necrosis. Hepatic steatosis was observed in animals acutely exposed to the compound. In the kidney, acute and/or subchronic intoxication with (PhTe)2 caused vacuolar degeneration or hydropic degeneration, atrophy and hypertrophy of renal tubules, forming hyaline casts and acute tubular necrosis. Pulmonar edema and vascular congestion were found in the lungs of animals exposed to (PhTe)2 50μmol/kg. Histological analysis of brain tissue revealed that acute and subchronic exposure to (PhTe)2 induced neurodegenerative disorders (neuropil vacuolation, status spongiosus) in the hippocampus and thalamus. The presence of neuronal nuclear pyknosis, the loss of neuronal viability and neuronal death were observed particularly in the hippocampus. Additionally, through immunohistochemistry, we identified glial reaction and loss of pre-synaptic activity in the thalamus and cortex, corresponding to areas of spongiform change. Our findings suggest that exposure to (PhTe)2, subcutaneously causes severe morphological changes in liver, kidney, lung and brain. The data obtained will contribute significantly to increase the knowledge on the toxicology of the compound (PhTe)2, since this is the first work that evaluates the histology of tissues of various organs after intoxication with the compound.
publishDate 2014
dc.date.none.fl_str_mv 2014-08-29
2015-04-01
2015-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv LUZ, Sônia Cristina Almeida da. ANATOMOPATHOLOGICAL EVALUATION OF LIVER, KIDNEY, LUNG AND BRAIN OF MICE EXPOSED TO DIPHENYL DITELLURIDE. 2014. 98 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.
http://repositorio.ufsm.br/handle/1/4480
dc.identifier.dark.fl_str_mv ark:/26339/001300000c39h
identifier_str_mv LUZ, Sônia Cristina Almeida da. ANATOMOPATHOLOGICAL EVALUATION OF LIVER, KIDNEY, LUNG AND BRAIN OF MICE EXPOSED TO DIPHENYL DITELLURIDE. 2014. 98 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.
ark:/26339/001300000c39h
url http://repositorio.ufsm.br/handle/1/4480
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
publisher.none.fl_str_mv Universidade Federal de Santa Maria
BR
Bioquímica
UFSM
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
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reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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