Aspectos epidemiológicos, clínicos, anatomopatológicos e imuno-histoquímicos do linfoma em bovinos (1965-2014)

• Autor(a) principal: Panziera, Welden
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Manancial - Repositório Digital da UFSM
• Texto Completo: http://repositorio.ufsm.br/handle/1/10193
• topic: Doenças de bovinos; Leucose enzoótica bovina; Linfoma; Linfossarcoma; Sistemas de classificação de tumores linfoides; Cattle diseases; Enzootic bovine leukosis; Lymphoma; Lymphosarcoma; Classification of lymphoid neoplasms; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA

The epidemiological, clinical and pathological aspects of 128 cases of bovine lymphoma are retrospectively described in this study (1965-2014).Out of the cases were the gender was informed (n=111), 84.7% of affected animals were females and 15.3% were males. Out of the cases were breed was informed (n=108), 63% of affected animals were Holstein cows. The age of affected cows (n=107) varied from 1 to 14 years, with most animals being adults (80.4%) with 5 to 8 years of age (57.9%). The most common clinical sign (n=89) was lymphadenomegaly (74.1%). Gross findings (n=125) included enlargement of lymph nodes in 71.2% of the cases; this finding was classified as localized in 89.6% of the cases and generalized in 10.3% of the cases. A retrospective study of 86 cases of bovine lymphoma classified as with diffuse pattern of distribution and verified by phenotypic (histology) and immunophenotypic (immunohistochemistry [IHC]) is presented. Based on these results, the lymphoma cases were classified by the Working Formulation (WF) of Non-Hodgkin s Lymphomas for Clinical Usage as: diffuse large non-cleaved cell (46.5%), diffuse large cleaved cell (33.7%), diffuse mixed small and large cell (4.6%), diffuse small cell plasmacytoid (7%), immunoblastic (3.5%), diffuse small cell intermediate (2.3%), diffuse small non-cleaved cell (1.2%), and diffuse small non-cleaved cell Burkitt s (1.2%). According to the IHC, 27 out of 86 (31.4%) lymphomas were positive to monoclonal antibody CD79αcy, used to detect B cells, and none were positive for polyclonal antibody CD3, used to detect T cells. Based on this, the B-cell type lymphomas were distributed as follows: diffuse large B-cell lymphoma (81.5%), large cell immunoblastic lymphoma (11.1%), and lymphoplasmacytoid lymphoma (7.4%), according the Revised European-American Classification of Lymphoid Neoplasms (REAL). The results of this retrospective study, similar to what has been described in other parts of the world, allow us to conclude that bovine lymphomas are basically diffuse and predominantly made of intermediate-grade, large cells, with cleaved or non-cleaved nuclei. These lymphomas are due to neoplastic proliferation of B cells and correspond to almost all (92.6%) to what is currently classified as diffuse large B-cell lymphoma by REAL.