Avaliação do estresse oxidativo em gestantes com diabetes mellitus pré-existente e gestacional
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/20975 |
Resumo: | During the normal gestational period, there is an elevation of oxidative metabolism due to the high demand for oxygen required by the mother, fetus and placenta. In pregnant women with additional pathologies, such as Diabetes Mellitus (DM), these circumstances may be even greater. DM is a metabolic disorder of diverse origin, characterized by hyperglycemia and changes in the metabolism of carbohydrates, proteins and fats, which are generated by the defect in the action and/or secretion of insulin. In pregnancy this pathology may be present in two ways: prior to pregnancy and developed in the gestational period, thus generated at different times in the body. Oxidative stress is said to be the state where the loss of balance between oxidants and antioxidants in the body occurs, primarily as a result of increased production of reactive oxygen species (ROS), and plays an important role in the pathogenesis of many diseases, including diabetes and complications. In this context, the enzyme delta- aminolevulinate-dehydratase (δ-ALA-D) is also inserted, since this enzyme may be inhibited in pro-oxidative situations, causing a decrease in the biosynthesis of compounds, as in the case of the heme group, and its substrate 5-aminolevulinic acid (ALA), which directly contributes to the elevation of this oxidative state by the accumulation of ROS. Considering the influence of oxidative stress on pregnancy and on DM, this study aimed to evaluate, in a comparative way, the oxidative and antioxidative profile, as well as the δ-ALA-D enzyme activity, in pregnant women with previous type 2 diabetes gestation (DM2), Gestational Diabetes Mellitus (DMG) and control group (healthy pregnant women). Oxidative stress parameters were analyzed by quantification of thiobarbituric acid reactive species (TBARS), protein thiol groups (P-SH) and non-protein (NP-SH) groups, vitamin C levels, and determination of enzyme activity catalase and δ-ALA-D, in the different groups of pregnant women. The levels of TBARS were higher, whereas NP-SH and vitamin C were decreased in pregnant women with DM, with no significant difference between the two groups. The P-SH levels were significantly reduced in pregnant women with DMG, and the activity of the enzyme catalase in the DM2 group. The activity of the δ-ALA-D enzyme was significantly reduced in samples from pregnant women with GDM, whereas in the group with DM2 prior to gestation, it was similar to the control. Thus, it is confirmed that oxidative stress is higher in pregnant women with DM, as well as diminished antioxidant defenses, and although both types of diabetes may be present during pregnancy, and are generated by carbohydrate intolerance, they are differentiated events and, therefore, produce different effects in the maternal organism. |
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Avaliação do estresse oxidativo em gestantes com diabetes mellitus pré-existente e gestacionalEvaluation of oxidative stress in pregnant women with pre- existing and gestational diabetes mellitusδ-ALA-DDiabetes mellitus na gestaçãoDiabetes mellitus pré-gestacionalGrávidasPerfil oxidativoDiabetes mellitus in pregnancyPre-gestational diabetes mellitusPregnantOxidative profileCNPQ::CIENCIAS DA SAUDE::FARMACIADuring the normal gestational period, there is an elevation of oxidative metabolism due to the high demand for oxygen required by the mother, fetus and placenta. In pregnant women with additional pathologies, such as Diabetes Mellitus (DM), these circumstances may be even greater. DM is a metabolic disorder of diverse origin, characterized by hyperglycemia and changes in the metabolism of carbohydrates, proteins and fats, which are generated by the defect in the action and/or secretion of insulin. In pregnancy this pathology may be present in two ways: prior to pregnancy and developed in the gestational period, thus generated at different times in the body. Oxidative stress is said to be the state where the loss of balance between oxidants and antioxidants in the body occurs, primarily as a result of increased production of reactive oxygen species (ROS), and plays an important role in the pathogenesis of many diseases, including diabetes and complications. In this context, the enzyme delta- aminolevulinate-dehydratase (δ-ALA-D) is also inserted, since this enzyme may be inhibited in pro-oxidative situations, causing a decrease in the biosynthesis of compounds, as in the case of the heme group, and its substrate 5-aminolevulinic acid (ALA), which directly contributes to the elevation of this oxidative state by the accumulation of ROS. Considering the influence of oxidative stress on pregnancy and on DM, this study aimed to evaluate, in a comparative way, the oxidative and antioxidative profile, as well as the δ-ALA-D enzyme activity, in pregnant women with previous type 2 diabetes gestation (DM2), Gestational Diabetes Mellitus (DMG) and control group (healthy pregnant women). Oxidative stress parameters were analyzed by quantification of thiobarbituric acid reactive species (TBARS), protein thiol groups (P-SH) and non-protein (NP-SH) groups, vitamin C levels, and determination of enzyme activity catalase and δ-ALA-D, in the different groups of pregnant women. The levels of TBARS were higher, whereas NP-SH and vitamin C were decreased in pregnant women with DM, with no significant difference between the two groups. The P-SH levels were significantly reduced in pregnant women with DMG, and the activity of the enzyme catalase in the DM2 group. The activity of the δ-ALA-D enzyme was significantly reduced in samples from pregnant women with GDM, whereas in the group with DM2 prior to gestation, it was similar to the control. Thus, it is confirmed that oxidative stress is higher in pregnant women with DM, as well as diminished antioxidant defenses, and although both types of diabetes may be present during pregnancy, and are generated by carbohydrate intolerance, they are differentiated events and, therefore, produce different effects in the maternal organism.Durante o período gestacional normal, há uma elevação do metabolismo oxidativo devido à grande demanda de oxigênio requisitada pela mãe, pelo feto e pela placenta. Em gestantes com patologias adicionais, como é o caso do Diabetes Mellitus (DM), essas circunstâncias podem estar ainda mais aumentadas. O DM trata-se de um distúrbio metabólico de origem diversa, caracterizado por hiperglicemia e alterações no metabolismo de carboidratos, proteínas e gorduras, que são geradas pelo defeito na ação e/ou secreção da insulina. Na gestação essa patologia pode estar presente de duas formas: prévia a gravidez e desenvolvida no período gestacional, geradas dessa forma em momentos distintos no organismo. O estresse oxidativo é dito como o estado onde ocorre a perda do equilíbrio entre oxidantes e antioxidantes no organismo, resultado principalmente da produção aumentada de espécies reativas de oxigênio (EROs), tendo um importante papel na patogênese de muitas doenças, incluindo o diabetes e suas complicações. Nesse contexto, também está inserida a enzima delta-aminolevulinato- desidratase (δ-ALA-D), pois esta pode estar inibida em situações pró-oxidativas, gerando diminuição da biossíntese de compostos, como é o caso do grupamento heme, e acúmulo de seu substrato ácido 5-aminolevulínico (ALA), que contribui diretamente para a elevação desse estado oxidativo pelo acúmulo de EROs. Considerando a influência do estresse oxidativo sobre a gestação e sobre o DM, este trabalho teve como objetivo, avaliar de forma comparativa, o perfil oxidativo e antioxidativo, bem como a atividade da enzima δ-ALA-D, em gestantes com diabetes tipo 2 prévia a gestação (DM2), Diabetes Mellitus Gestacional (DMG) e grupo controle (gestantes hígidas). Foram analisados parâmetros de estresse oxidativo através da quantificação das espécies reativas ao ácido tiobarbitúrico (TBARS), dos grupos tióis proteicos (P-SH) e não proteicos (NP-SH), os níveis de vitamina C, além da determinação da atividade das enzimas catalase e δ-ALA-D, nos diferentes grupos de gestantes. Os níveis de TBARS foram maiores, enquanto que os NP-SH e vitamina C, diminuídos, nas gestantes com DM, sem diferença significativa entre os dois grupos. Já os P-SH mostraram-se significativamente diminuídos nas gestantes com DMG, e a atividade da enzima catalase no grupo com DM2 prévia. A atividade da enzima δ-ALA-D mostrou-se significativamente diminuída em amostras de gestantes com DMG, enquanto no grupo com DM2 prévio a gestação, apresentou-se de modo semelhante ao controle. Dessa forma, confirma-se que o estresse oxidativo está mais elevado nas gestantes com DM, assim como as defesas antioxidantes diminuídas, e embora ambos tipos de diabetes possam estar presentes na gestação, e sejam gerados pela intolerância a carboidratos, eles são eventos diferenciados e, portanto, produzem efeitos distintos no organismo materno.Universidade Federal de Santa MariaBrasilFarmáciaUFSMPrograma de Pós-Graduação em Ciências FarmacêuticasCentro de Ciências da SaúdeBernasconi, Thissiane de Lima Gonçalveshttp://lattes.cnpq.br/1968750829053627Tatsch, EtianeBrandão, RicardoPaula, Hellen Lopes de2021-05-24T20:50:26Z2021-05-24T20:50:26Z2019-01-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/20975porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-10-07T17:14:53Zoai:repositorio.ufsm.br:1/20975Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-10-07T17:14:53Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Avaliação do estresse oxidativo em gestantes com diabetes mellitus pré-existente e gestacional Evaluation of oxidative stress in pregnant women with pre- existing and gestational diabetes mellitus |
title |
Avaliação do estresse oxidativo em gestantes com diabetes mellitus pré-existente e gestacional |
spellingShingle |
Avaliação do estresse oxidativo em gestantes com diabetes mellitus pré-existente e gestacional Paula, Hellen Lopes de δ-ALA-D Diabetes mellitus na gestação Diabetes mellitus pré-gestacional Grávidas Perfil oxidativo Diabetes mellitus in pregnancy Pre-gestational diabetes mellitus Pregnant Oxidative profile CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Avaliação do estresse oxidativo em gestantes com diabetes mellitus pré-existente e gestacional |
title_full |
Avaliação do estresse oxidativo em gestantes com diabetes mellitus pré-existente e gestacional |
title_fullStr |
Avaliação do estresse oxidativo em gestantes com diabetes mellitus pré-existente e gestacional |
title_full_unstemmed |
Avaliação do estresse oxidativo em gestantes com diabetes mellitus pré-existente e gestacional |
title_sort |
Avaliação do estresse oxidativo em gestantes com diabetes mellitus pré-existente e gestacional |
author |
Paula, Hellen Lopes de |
author_facet |
Paula, Hellen Lopes de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Bernasconi, Thissiane de Lima Gonçalves http://lattes.cnpq.br/1968750829053627 Tatsch, Etiane Brandão, Ricardo |
dc.contributor.author.fl_str_mv |
Paula, Hellen Lopes de |
dc.subject.por.fl_str_mv |
δ-ALA-D Diabetes mellitus na gestação Diabetes mellitus pré-gestacional Grávidas Perfil oxidativo Diabetes mellitus in pregnancy Pre-gestational diabetes mellitus Pregnant Oxidative profile CNPQ::CIENCIAS DA SAUDE::FARMACIA |
topic |
δ-ALA-D Diabetes mellitus na gestação Diabetes mellitus pré-gestacional Grávidas Perfil oxidativo Diabetes mellitus in pregnancy Pre-gestational diabetes mellitus Pregnant Oxidative profile CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
During the normal gestational period, there is an elevation of oxidative metabolism due to the high demand for oxygen required by the mother, fetus and placenta. In pregnant women with additional pathologies, such as Diabetes Mellitus (DM), these circumstances may be even greater. DM is a metabolic disorder of diverse origin, characterized by hyperglycemia and changes in the metabolism of carbohydrates, proteins and fats, which are generated by the defect in the action and/or secretion of insulin. In pregnancy this pathology may be present in two ways: prior to pregnancy and developed in the gestational period, thus generated at different times in the body. Oxidative stress is said to be the state where the loss of balance between oxidants and antioxidants in the body occurs, primarily as a result of increased production of reactive oxygen species (ROS), and plays an important role in the pathogenesis of many diseases, including diabetes and complications. In this context, the enzyme delta- aminolevulinate-dehydratase (δ-ALA-D) is also inserted, since this enzyme may be inhibited in pro-oxidative situations, causing a decrease in the biosynthesis of compounds, as in the case of the heme group, and its substrate 5-aminolevulinic acid (ALA), which directly contributes to the elevation of this oxidative state by the accumulation of ROS. Considering the influence of oxidative stress on pregnancy and on DM, this study aimed to evaluate, in a comparative way, the oxidative and antioxidative profile, as well as the δ-ALA-D enzyme activity, in pregnant women with previous type 2 diabetes gestation (DM2), Gestational Diabetes Mellitus (DMG) and control group (healthy pregnant women). Oxidative stress parameters were analyzed by quantification of thiobarbituric acid reactive species (TBARS), protein thiol groups (P-SH) and non-protein (NP-SH) groups, vitamin C levels, and determination of enzyme activity catalase and δ-ALA-D, in the different groups of pregnant women. The levels of TBARS were higher, whereas NP-SH and vitamin C were decreased in pregnant women with DM, with no significant difference between the two groups. The P-SH levels were significantly reduced in pregnant women with DMG, and the activity of the enzyme catalase in the DM2 group. The activity of the δ-ALA-D enzyme was significantly reduced in samples from pregnant women with GDM, whereas in the group with DM2 prior to gestation, it was similar to the control. Thus, it is confirmed that oxidative stress is higher in pregnant women with DM, as well as diminished antioxidant defenses, and although both types of diabetes may be present during pregnancy, and are generated by carbohydrate intolerance, they are differentiated events and, therefore, produce different effects in the maternal organism. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-11 2021-05-24T20:50:26Z 2021-05-24T20:50:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/20975 |
url |
http://repositorio.ufsm.br/handle/1/20975 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Farmácia UFSM Programa de Pós-Graduação em Ciências Farmacêuticas Centro de Ciências da Saúde |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Farmácia UFSM Programa de Pós-Graduação em Ciências Farmacêuticas Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922148673388544 |