Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium

Detalhes bibliográficos
Autor(a) principal: Rossi, Alexandre Guilherme Zabeu [UNIFESP]
Data de Publicação: 2010
Outros Autores: Gomes, Regina Célia Teixeira [UNIFESP], Simões, Manuel de Jesus [UNIFESP], Simões, Ricardo dos Santos [UNIFESP], Oliveira, Patricia B. [UNIFESP], Soares, Jose Maria [UNIFESP], Baracat, Edmund Chada [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/001300001813t
Texto Completo: http://dx.doi.org/10.1016/j.fertnstert.2009.02.021
http://repositorio.unifesp.br/handle/11600/32380
Resumo: Objective: To evaluate the effects of metoclopramide-induced hyperprolactinemia, on the prolactin receptor of murine endometrium.Design: Experimental study using the RNA extraction to detect tissue prolactin recepter isoforms by reverse-transcriptase polymerase chain reaction (RT-PCR).Setting: University-based laboratory.Animal(s): Seventy-two female swiss albino mice (Mus musculus), approximately 100 days old, were divided into six 12-animal groups: (Cl) nonoophorectomized mice given vehicle; (GII) nonoophorectomized mice treated with metoclopramide; (Gill) oophorectomized mice treated with metoclopramide; (GIV)oophorectomized mice treated with metoclopramide and 17 beta-estradiol; (GV) oophorectomized mice treated with metoclopramide and micronized progesterone; (GVI) oophorectomized mice treated with metoclopramide and a solution of 17 beta-estradiol and micronized progesterone.Intervention(s): Drugs were administered for 50 days. Following euthanasia, the middle portions of the uterine horns were removed, sectioned, and immediately frozen for RT-PCR procedures. Blood was collected for the dosage of prolactin and serum estrogen and progesterone using radioimmune assay.Main Outcome Measure(s): Identification of uterine prolactin receptor isoforms:Result(s): the PRL receptor and its isoform L were identified only in GI (control group) and GII (metoclopramide), the two groups with nonoophorectomized animals. the amount of PRL receptor mRNA and that of its isoform L from GII were the largest. No other isoforms of the prolactin receptor were identified in any of the groups.Conclusion(s): Our results suggest that replacement of estrogen and progestin may not increase the mRNA of endometrial PRL receptor in metoclopromide-induced hyperprolactinemia in rats after castration. (Fertil Steril (R) 2010;93:1643-9. (C)2010 by American Society for Reproductive Medicine.)
id UFSP_002abdf22c0d2caea70592533e67e978
oai_identifier_str oai:repositorio.unifesp.br/:11600/32380
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometriumHyperprolactinemiaprolactin receptorprolactinmouseestrogenendometriumObjective: To evaluate the effects of metoclopramide-induced hyperprolactinemia, on the prolactin receptor of murine endometrium.Design: Experimental study using the RNA extraction to detect tissue prolactin recepter isoforms by reverse-transcriptase polymerase chain reaction (RT-PCR).Setting: University-based laboratory.Animal(s): Seventy-two female swiss albino mice (Mus musculus), approximately 100 days old, were divided into six 12-animal groups: (Cl) nonoophorectomized mice given vehicle; (GII) nonoophorectomized mice treated with metoclopramide; (Gill) oophorectomized mice treated with metoclopramide; (GIV)oophorectomized mice treated with metoclopramide and 17 beta-estradiol; (GV) oophorectomized mice treated with metoclopramide and micronized progesterone; (GVI) oophorectomized mice treated with metoclopramide and a solution of 17 beta-estradiol and micronized progesterone.Intervention(s): Drugs were administered for 50 days. Following euthanasia, the middle portions of the uterine horns were removed, sectioned, and immediately frozen for RT-PCR procedures. Blood was collected for the dosage of prolactin and serum estrogen and progesterone using radioimmune assay.Main Outcome Measure(s): Identification of uterine prolactin receptor isoforms:Result(s): the PRL receptor and its isoform L were identified only in GI (control group) and GII (metoclopramide), the two groups with nonoophorectomized animals. the amount of PRL receptor mRNA and that of its isoform L from GII were the largest. No other isoforms of the prolactin receptor were identified in any of the groups.Conclusion(s): Our results suggest that replacement of estrogen and progestin may not increase the mRNA of endometrial PRL receptor in metoclopromide-induced hyperprolactinemia in rats after castration. (Fertil Steril (R) 2010;93:1643-9. (C)2010 by American Society for Reproductive Medicine.)Universidade Federal de São Paulo, Dept Gynecol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morphol, São Paulo, BrazilUniv São Paulo, Sch Med, Dept Obstet & Gynecol, BR-09500900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Gynecol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morphol, São Paulo, BrazilWeb of ScienceElsevier B.V.Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Rossi, Alexandre Guilherme Zabeu [UNIFESP]Gomes, Regina Célia Teixeira [UNIFESP]Simões, Manuel de Jesus [UNIFESP]Simões, Ricardo dos Santos [UNIFESP]Oliveira, Patricia B. [UNIFESP]Soares, Jose Maria [UNIFESP]Baracat, Edmund Chada [UNIFESP]2016-01-24T13:59:28Z2016-01-24T13:59:28Z2010-03-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1643-1649http://dx.doi.org/10.1016/j.fertnstert.2009.02.021Fertility and Sterility. New York: Elsevier B.V., v. 93, n. 5, p. 1643-1649, 2010.10.1016/j.fertnstert.2009.02.0210015-0282http://repositorio.unifesp.br/handle/11600/32380WOS:000276038700034ark:/48912/001300001813tengFertility and Sterilityinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-10-10T13:38:33Zoai:repositorio.unifesp.br/:11600/32380Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T21:02:43.244451Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium
title Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium
spellingShingle Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium
Rossi, Alexandre Guilherme Zabeu [UNIFESP]
Hyperprolactinemia
prolactin receptor
prolactin
mouse
estrogen
endometrium
title_short Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium
title_full Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium
title_fullStr Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium
title_full_unstemmed Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium
title_sort Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium
author Rossi, Alexandre Guilherme Zabeu [UNIFESP]
author_facet Rossi, Alexandre Guilherme Zabeu [UNIFESP]
Gomes, Regina Célia Teixeira [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Simões, Ricardo dos Santos [UNIFESP]
Oliveira, Patricia B. [UNIFESP]
Soares, Jose Maria [UNIFESP]
Baracat, Edmund Chada [UNIFESP]
author_role author
author2 Gomes, Regina Célia Teixeira [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Simões, Ricardo dos Santos [UNIFESP]
Oliveira, Patricia B. [UNIFESP]
Soares, Jose Maria [UNIFESP]
Baracat, Edmund Chada [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Rossi, Alexandre Guilherme Zabeu [UNIFESP]
Gomes, Regina Célia Teixeira [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Simões, Ricardo dos Santos [UNIFESP]
Oliveira, Patricia B. [UNIFESP]
Soares, Jose Maria [UNIFESP]
Baracat, Edmund Chada [UNIFESP]
dc.subject.por.fl_str_mv Hyperprolactinemia
prolactin receptor
prolactin
mouse
estrogen
endometrium
topic Hyperprolactinemia
prolactin receptor
prolactin
mouse
estrogen
endometrium
description Objective: To evaluate the effects of metoclopramide-induced hyperprolactinemia, on the prolactin receptor of murine endometrium.Design: Experimental study using the RNA extraction to detect tissue prolactin recepter isoforms by reverse-transcriptase polymerase chain reaction (RT-PCR).Setting: University-based laboratory.Animal(s): Seventy-two female swiss albino mice (Mus musculus), approximately 100 days old, were divided into six 12-animal groups: (Cl) nonoophorectomized mice given vehicle; (GII) nonoophorectomized mice treated with metoclopramide; (Gill) oophorectomized mice treated with metoclopramide; (GIV)oophorectomized mice treated with metoclopramide and 17 beta-estradiol; (GV) oophorectomized mice treated with metoclopramide and micronized progesterone; (GVI) oophorectomized mice treated with metoclopramide and a solution of 17 beta-estradiol and micronized progesterone.Intervention(s): Drugs were administered for 50 days. Following euthanasia, the middle portions of the uterine horns were removed, sectioned, and immediately frozen for RT-PCR procedures. Blood was collected for the dosage of prolactin and serum estrogen and progesterone using radioimmune assay.Main Outcome Measure(s): Identification of uterine prolactin receptor isoforms:Result(s): the PRL receptor and its isoform L were identified only in GI (control group) and GII (metoclopramide), the two groups with nonoophorectomized animals. the amount of PRL receptor mRNA and that of its isoform L from GII were the largest. No other isoforms of the prolactin receptor were identified in any of the groups.Conclusion(s): Our results suggest that replacement of estrogen and progestin may not increase the mRNA of endometrial PRL receptor in metoclopromide-induced hyperprolactinemia in rats after castration. (Fertil Steril (R) 2010;93:1643-9. (C)2010 by American Society for Reproductive Medicine.)
publishDate 2010
dc.date.none.fl_str_mv 2010-03-15
2016-01-24T13:59:28Z
2016-01-24T13:59:28Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.fertnstert.2009.02.021
Fertility and Sterility. New York: Elsevier B.V., v. 93, n. 5, p. 1643-1649, 2010.
10.1016/j.fertnstert.2009.02.021
0015-0282
http://repositorio.unifesp.br/handle/11600/32380
WOS:000276038700034
dc.identifier.dark.fl_str_mv ark:/48912/001300001813t
url http://dx.doi.org/10.1016/j.fertnstert.2009.02.021
http://repositorio.unifesp.br/handle/11600/32380
identifier_str_mv Fertility and Sterility. New York: Elsevier B.V., v. 93, n. 5, p. 1643-1649, 2010.
10.1016/j.fertnstert.2009.02.021
0015-0282
WOS:000276038700034
ark:/48912/001300001813t
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fertility and Sterility
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 1643-1649
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1818602591794233344