Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
dARK ID: | ark:/48912/001300001813t |
Texto Completo: | http://dx.doi.org/10.1016/j.fertnstert.2009.02.021 http://repositorio.unifesp.br/handle/11600/32380 |
Resumo: | Objective: To evaluate the effects of metoclopramide-induced hyperprolactinemia, on the prolactin receptor of murine endometrium.Design: Experimental study using the RNA extraction to detect tissue prolactin recepter isoforms by reverse-transcriptase polymerase chain reaction (RT-PCR).Setting: University-based laboratory.Animal(s): Seventy-two female swiss albino mice (Mus musculus), approximately 100 days old, were divided into six 12-animal groups: (Cl) nonoophorectomized mice given vehicle; (GII) nonoophorectomized mice treated with metoclopramide; (Gill) oophorectomized mice treated with metoclopramide; (GIV)oophorectomized mice treated with metoclopramide and 17 beta-estradiol; (GV) oophorectomized mice treated with metoclopramide and micronized progesterone; (GVI) oophorectomized mice treated with metoclopramide and a solution of 17 beta-estradiol and micronized progesterone.Intervention(s): Drugs were administered for 50 days. Following euthanasia, the middle portions of the uterine horns were removed, sectioned, and immediately frozen for RT-PCR procedures. Blood was collected for the dosage of prolactin and serum estrogen and progesterone using radioimmune assay.Main Outcome Measure(s): Identification of uterine prolactin receptor isoforms:Result(s): the PRL receptor and its isoform L were identified only in GI (control group) and GII (metoclopramide), the two groups with nonoophorectomized animals. the amount of PRL receptor mRNA and that of its isoform L from GII were the largest. No other isoforms of the prolactin receptor were identified in any of the groups.Conclusion(s): Our results suggest that replacement of estrogen and progestin may not increase the mRNA of endometrial PRL receptor in metoclopromide-induced hyperprolactinemia in rats after castration. (Fertil Steril (R) 2010;93:1643-9. (C)2010 by American Society for Reproductive Medicine.) |
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Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometriumHyperprolactinemiaprolactin receptorprolactinmouseestrogenendometriumObjective: To evaluate the effects of metoclopramide-induced hyperprolactinemia, on the prolactin receptor of murine endometrium.Design: Experimental study using the RNA extraction to detect tissue prolactin recepter isoforms by reverse-transcriptase polymerase chain reaction (RT-PCR).Setting: University-based laboratory.Animal(s): Seventy-two female swiss albino mice (Mus musculus), approximately 100 days old, were divided into six 12-animal groups: (Cl) nonoophorectomized mice given vehicle; (GII) nonoophorectomized mice treated with metoclopramide; (Gill) oophorectomized mice treated with metoclopramide; (GIV)oophorectomized mice treated with metoclopramide and 17 beta-estradiol; (GV) oophorectomized mice treated with metoclopramide and micronized progesterone; (GVI) oophorectomized mice treated with metoclopramide and a solution of 17 beta-estradiol and micronized progesterone.Intervention(s): Drugs were administered for 50 days. Following euthanasia, the middle portions of the uterine horns were removed, sectioned, and immediately frozen for RT-PCR procedures. Blood was collected for the dosage of prolactin and serum estrogen and progesterone using radioimmune assay.Main Outcome Measure(s): Identification of uterine prolactin receptor isoforms:Result(s): the PRL receptor and its isoform L were identified only in GI (control group) and GII (metoclopramide), the two groups with nonoophorectomized animals. the amount of PRL receptor mRNA and that of its isoform L from GII were the largest. No other isoforms of the prolactin receptor were identified in any of the groups.Conclusion(s): Our results suggest that replacement of estrogen and progestin may not increase the mRNA of endometrial PRL receptor in metoclopromide-induced hyperprolactinemia in rats after castration. (Fertil Steril (R) 2010;93:1643-9. (C)2010 by American Society for Reproductive Medicine.)Universidade Federal de São Paulo, Dept Gynecol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morphol, São Paulo, BrazilUniv São Paulo, Sch Med, Dept Obstet & Gynecol, BR-09500900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Gynecol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Morphol, São Paulo, BrazilWeb of ScienceElsevier B.V.Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Rossi, Alexandre Guilherme Zabeu [UNIFESP]Gomes, Regina Célia Teixeira [UNIFESP]Simões, Manuel de Jesus [UNIFESP]Simões, Ricardo dos Santos [UNIFESP]Oliveira, Patricia B. [UNIFESP]Soares, Jose Maria [UNIFESP]Baracat, Edmund Chada [UNIFESP]2016-01-24T13:59:28Z2016-01-24T13:59:28Z2010-03-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1643-1649http://dx.doi.org/10.1016/j.fertnstert.2009.02.021Fertility and Sterility. New York: Elsevier B.V., v. 93, n. 5, p. 1643-1649, 2010.10.1016/j.fertnstert.2009.02.0210015-0282http://repositorio.unifesp.br/handle/11600/32380WOS:000276038700034ark:/48912/001300001813tengFertility and Sterilityinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-10-10T13:38:33Zoai:repositorio.unifesp.br/:11600/32380Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T21:02:43.244451Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium |
title |
Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium |
spellingShingle |
Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium Rossi, Alexandre Guilherme Zabeu [UNIFESP] Hyperprolactinemia prolactin receptor prolactin mouse estrogen endometrium |
title_short |
Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium |
title_full |
Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium |
title_fullStr |
Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium |
title_full_unstemmed |
Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium |
title_sort |
Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium |
author |
Rossi, Alexandre Guilherme Zabeu [UNIFESP] |
author_facet |
Rossi, Alexandre Guilherme Zabeu [UNIFESP] Gomes, Regina Célia Teixeira [UNIFESP] Simões, Manuel de Jesus [UNIFESP] Simões, Ricardo dos Santos [UNIFESP] Oliveira, Patricia B. [UNIFESP] Soares, Jose Maria [UNIFESP] Baracat, Edmund Chada [UNIFESP] |
author_role |
author |
author2 |
Gomes, Regina Célia Teixeira [UNIFESP] Simões, Manuel de Jesus [UNIFESP] Simões, Ricardo dos Santos [UNIFESP] Oliveira, Patricia B. [UNIFESP] Soares, Jose Maria [UNIFESP] Baracat, Edmund Chada [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Rossi, Alexandre Guilherme Zabeu [UNIFESP] Gomes, Regina Célia Teixeira [UNIFESP] Simões, Manuel de Jesus [UNIFESP] Simões, Ricardo dos Santos [UNIFESP] Oliveira, Patricia B. [UNIFESP] Soares, Jose Maria [UNIFESP] Baracat, Edmund Chada [UNIFESP] |
dc.subject.por.fl_str_mv |
Hyperprolactinemia prolactin receptor prolactin mouse estrogen endometrium |
topic |
Hyperprolactinemia prolactin receptor prolactin mouse estrogen endometrium |
description |
Objective: To evaluate the effects of metoclopramide-induced hyperprolactinemia, on the prolactin receptor of murine endometrium.Design: Experimental study using the RNA extraction to detect tissue prolactin recepter isoforms by reverse-transcriptase polymerase chain reaction (RT-PCR).Setting: University-based laboratory.Animal(s): Seventy-two female swiss albino mice (Mus musculus), approximately 100 days old, were divided into six 12-animal groups: (Cl) nonoophorectomized mice given vehicle; (GII) nonoophorectomized mice treated with metoclopramide; (Gill) oophorectomized mice treated with metoclopramide; (GIV)oophorectomized mice treated with metoclopramide and 17 beta-estradiol; (GV) oophorectomized mice treated with metoclopramide and micronized progesterone; (GVI) oophorectomized mice treated with metoclopramide and a solution of 17 beta-estradiol and micronized progesterone.Intervention(s): Drugs were administered for 50 days. Following euthanasia, the middle portions of the uterine horns were removed, sectioned, and immediately frozen for RT-PCR procedures. Blood was collected for the dosage of prolactin and serum estrogen and progesterone using radioimmune assay.Main Outcome Measure(s): Identification of uterine prolactin receptor isoforms:Result(s): the PRL receptor and its isoform L were identified only in GI (control group) and GII (metoclopramide), the two groups with nonoophorectomized animals. the amount of PRL receptor mRNA and that of its isoform L from GII were the largest. No other isoforms of the prolactin receptor were identified in any of the groups.Conclusion(s): Our results suggest that replacement of estrogen and progestin may not increase the mRNA of endometrial PRL receptor in metoclopromide-induced hyperprolactinemia in rats after castration. (Fertil Steril (R) 2010;93:1643-9. (C)2010 by American Society for Reproductive Medicine.) |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03-15 2016-01-24T13:59:28Z 2016-01-24T13:59:28Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fertnstert.2009.02.021 Fertility and Sterility. New York: Elsevier B.V., v. 93, n. 5, p. 1643-1649, 2010. 10.1016/j.fertnstert.2009.02.021 0015-0282 http://repositorio.unifesp.br/handle/11600/32380 WOS:000276038700034 |
dc.identifier.dark.fl_str_mv |
ark:/48912/001300001813t |
url |
http://dx.doi.org/10.1016/j.fertnstert.2009.02.021 http://repositorio.unifesp.br/handle/11600/32380 |
identifier_str_mv |
Fertility and Sterility. New York: Elsevier B.V., v. 93, n. 5, p. 1643-1649, 2010. 10.1016/j.fertnstert.2009.02.021 0015-0282 WOS:000276038700034 ark:/48912/001300001813t |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fertility and Sterility |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
dc.format.none.fl_str_mv |
1643-1649 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1818602591794233344 |