Relationship between fluid-attenuated inversion-recovery (FLAIR) signal intensity and inflammatory mediator's levels in the hippocampus of patients with temporal lobe epilepsy and mesial temporal sclerosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1590/S0004-282X2011000100018 http://repositorio.unifesp.br/handle/11600/6317 |
Resumo: | We investigated a relationship between the FLAIR signal found in mesial temporal sclerosis (MTS) and inflammation. Twenty nine patients were selected through clinical and MRI analysis and submitted to cortico-amygdalo-hippocampectomy to seizure control. Glutamate, TNFα, IL1, nitric oxide (NO) levels and immunostaining against IL1β and CD45 was performed. Control tissues (n=10) were obtained after autopsy of patients without neurological disorders. The glutamate was decreased in the temporal lobe epilepsy (TLE) -MTS group (p<0.001), suggesting increased release of this neurotransmitter. The IL1β and TNFα were increased in the hippocampus (p<0.05) demonstrating an active inflammatory process. A positive linear correlation between FLAIR signal and NO and IL1β levels and a negative linear correlation between FLAIR signal and glutamate concentration was found. Lymphocytes infiltrates were present in hippocampi of TLE patients. These data showed an association between hippocampal signal alteration and increased inflammatory markers in TLE-MTS. |
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Relationship between fluid-attenuated inversion-recovery (FLAIR) signal intensity and inflammatory mediator's levels in the hippocampus of patients with temporal lobe epilepsy and mesial temporal sclerosisCorrelação da intensidade do sinal em FLAIR e os níveis de mediadores inflamatórios no hipocampo de pacientes com epilepsia do lobo temporal e esclerose mesial temporalFLAIR hippocampal signalcytokinesNOglutamate and hippocampal sclerosissinal hipocampal em FLAIRcitocinasNOglutamatoesclerose hipocampalWe investigated a relationship between the FLAIR signal found in mesial temporal sclerosis (MTS) and inflammation. Twenty nine patients were selected through clinical and MRI analysis and submitted to cortico-amygdalo-hippocampectomy to seizure control. Glutamate, TNFα, IL1, nitric oxide (NO) levels and immunostaining against IL1β and CD45 was performed. Control tissues (n=10) were obtained after autopsy of patients without neurological disorders. The glutamate was decreased in the temporal lobe epilepsy (TLE) -MTS group (p<0.001), suggesting increased release of this neurotransmitter. The IL1β and TNFα were increased in the hippocampus (p<0.05) demonstrating an active inflammatory process. A positive linear correlation between FLAIR signal and NO and IL1β levels and a negative linear correlation between FLAIR signal and glutamate concentration was found. Lymphocytes infiltrates were present in hippocampi of TLE patients. These data showed an association between hippocampal signal alteration and increased inflammatory markers in TLE-MTS.Este estudo foi delineado para investigar a presença de relação entre a intensidade de sinal em FLAIR e níveis de citocinas, óxido nítrico (NO) e glutamato no hipocampo de pacientes com epilepsia do lobo temporal refratária, associada com esclerose mesial (TLE-MTS). Vinte e nove pacientes foram selecionados através de análise clínica e de ressonância magnética (RM) que foram submetidos a cortico-amigdalo-hipocampectomia para o controle das crises. Os níveis de glutamato foram avaliados por HPLC, as citocinas TNFα e IL1β por ELISA e os níveis de NO via NO system. Avaliamos também por imuno-histoquímica a expressão de IL1β e CD45 em tecidos controles e com esclerose. Tecido controle foi obtido após autópsia de indivíduos mortos sem disfunções inflamatórias e neurológicas (n=10). A concentração de glutamato se mostrou reduzida no tecido TLE-MTS (p<0,001) sugerindo aumento na liberação desse neurotransmissor. TNFα e IL1β também apresentaram níveis elevados no hipocampo dos pacientes (p<0,05), demonstrando um processo inflamatório crônico. Houve uma correlação linear positiva entre a intensidade do sinal em FLAIR e os níveis de NO e IL1β. Em contraste, uma correlação linear negativa foi encontrada entre a intensidade do sinal em FLAIR e níveis de glutamato no hipocampo com esclerose. Infiltrado linfocitário hipocampal também foi visualizado pela imuno-marcação com CD45 em pacientes com TLE-MTS. Esses dados mostraram uma associação entre alteração de sinal na RM e marcadores inflamatórios em pacientes com TLE-MTS.UNIFESP Neurosurgery DepartmentUNIFESP Diagnosis and Image DepartmentUNIFESP Medicine DepartmentUniversity of São Paulo Hearth InstituteUNIFESP Biochemistry DepartmentUNIFESP, Neurosurgery DepartmentUNIFESP, Diagnosis and Image DepartmentUNIFESP, Medicine DepartmentUNIFESP, Biochemistry DepartmentSciELOAcademia Brasileira de Neurologia - ABNEUROUniversidade Federal de São Paulo (UNIFESP)University of São Paulo Hearth InstituteVarella, Pedro Paulo Vasconcellos [UNIFESP]Santiago, Joselita Ferreira Carvalho [UNIFESP]Carrete Junior, Henrique [UNIFESP]Higa, Elisa Mieko Suemitsu [UNIFESP]Yacubian, Elza Márcia Targas [UNIFESP]Centeno, Ricardo Silva [UNIFESP]Caboclo, Luís Otávio Sales Ferreira [UNIFESP]Castro Neto, Eduardo Ferreira de [UNIFESP]Canzian, MauroAmado, Débora [UNIFESP]Cavalheiro, Esper Abrão [UNIFESP]Naffah-Mazzacoratti, Maria da Graca [UNIFESP]2015-06-14T13:42:56Z2015-06-14T13:42:56Z2011-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion91-99application/pdfhttp://dx.doi.org/10.1590/S0004-282X2011000100018Arquivos de Neuro-Psiquiatria. Academia Brasileira de Neurologia - ABNEURO, v. 69, n. 1, p. 91-99, 2011.10.1590/S0004-282X2011000100018S0004-282X2011000100018.pdf0004-282XS0004-282X2011000100018http://repositorio.unifesp.br/handle/11600/6317WOS:000287809600018engArquivos de Neuro-Psiquiatriainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-05T17:51:20Zoai:repositorio.unifesp.br/:11600/6317Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-05T17:51:20Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Relationship between fluid-attenuated inversion-recovery (FLAIR) signal intensity and inflammatory mediator's levels in the hippocampus of patients with temporal lobe epilepsy and mesial temporal sclerosis Correlação da intensidade do sinal em FLAIR e os níveis de mediadores inflamatórios no hipocampo de pacientes com epilepsia do lobo temporal e esclerose mesial temporal |
| title |
Relationship between fluid-attenuated inversion-recovery (FLAIR) signal intensity and inflammatory mediator's levels in the hippocampus of patients with temporal lobe epilepsy and mesial temporal sclerosis |
| spellingShingle |
Relationship between fluid-attenuated inversion-recovery (FLAIR) signal intensity and inflammatory mediator's levels in the hippocampus of patients with temporal lobe epilepsy and mesial temporal sclerosis Varella, Pedro Paulo Vasconcellos [UNIFESP] FLAIR hippocampal signal cytokines NO glutamate and hippocampal sclerosis sinal hipocampal em FLAIR citocinas NO glutamato esclerose hipocampal |
| title_short |
Relationship between fluid-attenuated inversion-recovery (FLAIR) signal intensity and inflammatory mediator's levels in the hippocampus of patients with temporal lobe epilepsy and mesial temporal sclerosis |
| title_full |
Relationship between fluid-attenuated inversion-recovery (FLAIR) signal intensity and inflammatory mediator's levels in the hippocampus of patients with temporal lobe epilepsy and mesial temporal sclerosis |
| title_fullStr |
Relationship between fluid-attenuated inversion-recovery (FLAIR) signal intensity and inflammatory mediator's levels in the hippocampus of patients with temporal lobe epilepsy and mesial temporal sclerosis |
| title_full_unstemmed |
Relationship between fluid-attenuated inversion-recovery (FLAIR) signal intensity and inflammatory mediator's levels in the hippocampus of patients with temporal lobe epilepsy and mesial temporal sclerosis |
| title_sort |
Relationship between fluid-attenuated inversion-recovery (FLAIR) signal intensity and inflammatory mediator's levels in the hippocampus of patients with temporal lobe epilepsy and mesial temporal sclerosis |
| author |
Varella, Pedro Paulo Vasconcellos [UNIFESP] |
| author_facet |
Varella, Pedro Paulo Vasconcellos [UNIFESP] Santiago, Joselita Ferreira Carvalho [UNIFESP] Carrete Junior, Henrique [UNIFESP] Higa, Elisa Mieko Suemitsu [UNIFESP] Yacubian, Elza Márcia Targas [UNIFESP] Centeno, Ricardo Silva [UNIFESP] Caboclo, Luís Otávio Sales Ferreira [UNIFESP] Castro Neto, Eduardo Ferreira de [UNIFESP] Canzian, Mauro Amado, Débora [UNIFESP] Cavalheiro, Esper Abrão [UNIFESP] Naffah-Mazzacoratti, Maria da Graca [UNIFESP] |
| author_role |
author |
| author2 |
Santiago, Joselita Ferreira Carvalho [UNIFESP] Carrete Junior, Henrique [UNIFESP] Higa, Elisa Mieko Suemitsu [UNIFESP] Yacubian, Elza Márcia Targas [UNIFESP] Centeno, Ricardo Silva [UNIFESP] Caboclo, Luís Otávio Sales Ferreira [UNIFESP] Castro Neto, Eduardo Ferreira de [UNIFESP] Canzian, Mauro Amado, Débora [UNIFESP] Cavalheiro, Esper Abrão [UNIFESP] Naffah-Mazzacoratti, Maria da Graca [UNIFESP] |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) University of São Paulo Hearth Institute |
| dc.contributor.author.fl_str_mv |
Varella, Pedro Paulo Vasconcellos [UNIFESP] Santiago, Joselita Ferreira Carvalho [UNIFESP] Carrete Junior, Henrique [UNIFESP] Higa, Elisa Mieko Suemitsu [UNIFESP] Yacubian, Elza Márcia Targas [UNIFESP] Centeno, Ricardo Silva [UNIFESP] Caboclo, Luís Otávio Sales Ferreira [UNIFESP] Castro Neto, Eduardo Ferreira de [UNIFESP] Canzian, Mauro Amado, Débora [UNIFESP] Cavalheiro, Esper Abrão [UNIFESP] Naffah-Mazzacoratti, Maria da Graca [UNIFESP] |
| dc.subject.por.fl_str_mv |
FLAIR hippocampal signal cytokines NO glutamate and hippocampal sclerosis sinal hipocampal em FLAIR citocinas NO glutamato esclerose hipocampal |
| topic |
FLAIR hippocampal signal cytokines NO glutamate and hippocampal sclerosis sinal hipocampal em FLAIR citocinas NO glutamato esclerose hipocampal |
| description |
We investigated a relationship between the FLAIR signal found in mesial temporal sclerosis (MTS) and inflammation. Twenty nine patients were selected through clinical and MRI analysis and submitted to cortico-amygdalo-hippocampectomy to seizure control. Glutamate, TNFα, IL1, nitric oxide (NO) levels and immunostaining against IL1β and CD45 was performed. Control tissues (n=10) were obtained after autopsy of patients without neurological disorders. The glutamate was decreased in the temporal lobe epilepsy (TLE) -MTS group (p<0.001), suggesting increased release of this neurotransmitter. The IL1β and TNFα were increased in the hippocampus (p<0.05) demonstrating an active inflammatory process. A positive linear correlation between FLAIR signal and NO and IL1β levels and a negative linear correlation between FLAIR signal and glutamate concentration was found. Lymphocytes infiltrates were present in hippocampi of TLE patients. These data showed an association between hippocampal signal alteration and increased inflammatory markers in TLE-MTS. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011-02-01 2015-06-14T13:42:56Z 2015-06-14T13:42:56Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0004-282X2011000100018 Arquivos de Neuro-Psiquiatria. Academia Brasileira de Neurologia - ABNEURO, v. 69, n. 1, p. 91-99, 2011. 10.1590/S0004-282X2011000100018 S0004-282X2011000100018.pdf 0004-282X S0004-282X2011000100018 http://repositorio.unifesp.br/handle/11600/6317 WOS:000287809600018 |
| url |
http://dx.doi.org/10.1590/S0004-282X2011000100018 http://repositorio.unifesp.br/handle/11600/6317 |
| identifier_str_mv |
Arquivos de Neuro-Psiquiatria. Academia Brasileira de Neurologia - ABNEURO, v. 69, n. 1, p. 91-99, 2011. 10.1590/S0004-282X2011000100018 S0004-282X2011000100018.pdf 0004-282X S0004-282X2011000100018 WOS:000287809600018 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Arquivos de Neuro-Psiquiatria |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
91-99 application/pdf |
| dc.publisher.none.fl_str_mv |
Academia Brasileira de Neurologia - ABNEURO |
| publisher.none.fl_str_mv |
Academia Brasileira de Neurologia - ABNEURO |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268352557744128 |