Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage

Detalhes bibliográficos
Autor(a) principal: Guimaraes, Natalia S. S.
Data de Publicação: 2012
Outros Autores: Mello, Joyce C., Paiva, Juliana S., Bueno, Paula C. P., Berretta, Andresa A., Torquato, Ricardo José Soares [UNIFESP], Nantes, Iseli L., Rodrigues, Tiago
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.fct.2011.11.014
http://repositorio.unifesp.br/handle/11600/34633
Resumo: Baccharis dracunculifolia DC (Asteraceae) is the main botanical source used by honeybees to produce Brazilian green propolis whose hepatoprotective properties have been already described. in this work we investigated the protective effects of the glycolic extract of B. dracunculifolia (GEBd) against oxidative stress in isolated rat liver mitochondria (RLM). the GEBd was prepared by fractionated percolation using propylene glycol as solvent. the total phenols and flavonoids, which are substances with recognized antioxidant action, were quantified in GEBd and the phytochemical analysis was carried out by HPLC. GEBd exhibited significant scavenger activity towards DPPH radicals and superoxide anions in a concentration-dependent manner, and also a Fe2+ chelating activity. GEBd decreased the basal H2O2 generation and the Fe2+- or t-BuOOH-induced ROS production in isolated mitochondria. Lipid oxidation of mitochondrial membranes, protein thiol groups and GSH oxidation were also prevented by GEBd. This shows that B. dracunculifolia exhibit potent antioxidant activity protecting liver mitochondria against oxidative damage and such action probably contribute to the antioxidant and hepatoprotective effects of green propolis. (C) 2011 Elsevier B.V. All rights reserved.
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spelling Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damageBaccharis dracunculifoliaAntioxidantFree radicalsHepatoprotectionMitochondriaOxidative stressBaccharis dracunculifolia DC (Asteraceae) is the main botanical source used by honeybees to produce Brazilian green propolis whose hepatoprotective properties have been already described. in this work we investigated the protective effects of the glycolic extract of B. dracunculifolia (GEBd) against oxidative stress in isolated rat liver mitochondria (RLM). the GEBd was prepared by fractionated percolation using propylene glycol as solvent. the total phenols and flavonoids, which are substances with recognized antioxidant action, were quantified in GEBd and the phytochemical analysis was carried out by HPLC. GEBd exhibited significant scavenger activity towards DPPH radicals and superoxide anions in a concentration-dependent manner, and also a Fe2+ chelating activity. GEBd decreased the basal H2O2 generation and the Fe2+- or t-BuOOH-induced ROS production in isolated mitochondria. Lipid oxidation of mitochondrial membranes, protein thiol groups and GSH oxidation were also prevented by GEBd. This shows that B. dracunculifolia exhibit potent antioxidant activity protecting liver mitochondria against oxidative damage and such action probably contribute to the antioxidant and hepatoprotective effects of green propolis. (C) 2011 Elsevier B.V. All rights reserved.Univ Mogi das Cruzes UMC, Ctr Interdisciplinar Invest Bioquim CIIB, Mogi Das Cruzes, SP, BrazilUniv Estadual Paulista UNESP, Inst Quim, São Paulo, BrazilFac Ciencias Farmaceut Ribeirao Preto FCFRP USP, São Paulo, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Bioquim, São Paulo, BrazilUniv Fed ABC UFABC, Ctr Ciencias Nat & Humanas CCNH, Santo Andre, SP, BrazilUniversidade Federal de São Paulo UNIFESP, Dept Bioquim, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 2008/01724-4FAPESP: 2008/07246-7CNPq: 301672/2009-1CNPq: 136255/2009-4Elsevier B.V.Univ Mogi das Cruzes UMCUniv Estadual Paulista UNESPUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Universidade Federal do ABC (UFABC)Guimaraes, Natalia S. S.Mello, Joyce C.Paiva, Juliana S.Bueno, Paula C. P.Berretta, Andresa A.Torquato, Ricardo José Soares [UNIFESP]Nantes, Iseli L.Rodrigues, Tiago2016-01-24T14:17:56Z2016-01-24T14:17:56Z2012-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1091-1097application/pdfhttp://dx.doi.org/10.1016/j.fct.2011.11.014Food and Chemical Toxicology. Oxford: Pergamon-Elsevier B.V., v. 50, n. 3-4, p. 1091-1097, 2012.10.1016/j.fct.2011.11.014WOS000303284600091.pdf0278-6915http://repositorio.unifesp.br/handle/11600/34633WOS:000303284600091engFood and Chemical Toxicologyinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-01T00:23:50Zoai:repositorio.unifesp.br/:11600/34633Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-01T00:23:50Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage
title Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage
spellingShingle Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage
Guimaraes, Natalia S. S.
Baccharis dracunculifolia
Antioxidant
Free radicals
Hepatoprotection
Mitochondria
Oxidative stress
title_short Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage
title_full Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage
title_fullStr Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage
title_full_unstemmed Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage
title_sort Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage
author Guimaraes, Natalia S. S.
author_facet Guimaraes, Natalia S. S.
Mello, Joyce C.
Paiva, Juliana S.
Bueno, Paula C. P.
Berretta, Andresa A.
Torquato, Ricardo José Soares [UNIFESP]
Nantes, Iseli L.
Rodrigues, Tiago
author_role author
author2 Mello, Joyce C.
Paiva, Juliana S.
Bueno, Paula C. P.
Berretta, Andresa A.
Torquato, Ricardo José Soares [UNIFESP]
Nantes, Iseli L.
Rodrigues, Tiago
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Mogi das Cruzes UMC
Univ Estadual Paulista UNESP
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do ABC (UFABC)
dc.contributor.author.fl_str_mv Guimaraes, Natalia S. S.
Mello, Joyce C.
Paiva, Juliana S.
Bueno, Paula C. P.
Berretta, Andresa A.
Torquato, Ricardo José Soares [UNIFESP]
Nantes, Iseli L.
Rodrigues, Tiago
dc.subject.por.fl_str_mv Baccharis dracunculifolia
Antioxidant
Free radicals
Hepatoprotection
Mitochondria
Oxidative stress
topic Baccharis dracunculifolia
Antioxidant
Free radicals
Hepatoprotection
Mitochondria
Oxidative stress
description Baccharis dracunculifolia DC (Asteraceae) is the main botanical source used by honeybees to produce Brazilian green propolis whose hepatoprotective properties have been already described. in this work we investigated the protective effects of the glycolic extract of B. dracunculifolia (GEBd) against oxidative stress in isolated rat liver mitochondria (RLM). the GEBd was prepared by fractionated percolation using propylene glycol as solvent. the total phenols and flavonoids, which are substances with recognized antioxidant action, were quantified in GEBd and the phytochemical analysis was carried out by HPLC. GEBd exhibited significant scavenger activity towards DPPH radicals and superoxide anions in a concentration-dependent manner, and also a Fe2+ chelating activity. GEBd decreased the basal H2O2 generation and the Fe2+- or t-BuOOH-induced ROS production in isolated mitochondria. Lipid oxidation of mitochondrial membranes, protein thiol groups and GSH oxidation were also prevented by GEBd. This shows that B. dracunculifolia exhibit potent antioxidant activity protecting liver mitochondria against oxidative damage and such action probably contribute to the antioxidant and hepatoprotective effects of green propolis. (C) 2011 Elsevier B.V. All rights reserved.
publishDate 2012
dc.date.none.fl_str_mv 2012-03-01
2016-01-24T14:17:56Z
2016-01-24T14:17:56Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.fct.2011.11.014
Food and Chemical Toxicology. Oxford: Pergamon-Elsevier B.V., v. 50, n. 3-4, p. 1091-1097, 2012.
10.1016/j.fct.2011.11.014
WOS000303284600091.pdf
0278-6915
http://repositorio.unifesp.br/handle/11600/34633
WOS:000303284600091
url http://dx.doi.org/10.1016/j.fct.2011.11.014
http://repositorio.unifesp.br/handle/11600/34633
identifier_str_mv Food and Chemical Toxicology. Oxford: Pergamon-Elsevier B.V., v. 50, n. 3-4, p. 1091-1097, 2012.
10.1016/j.fct.2011.11.014
WOS000303284600091.pdf
0278-6915
WOS:000303284600091
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Food and Chemical Toxicology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 1091-1097
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268447498960896

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