Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression

Detalhes bibliográficos
Autor(a) principal: Varela, Patricia [UNIFESP]
Data de Publicação: 2014
Outros Autores: Escosteguy-Neto, João Carlos [UNIFESP], Coelho, Carolina Tesone [UNIFESP], Mello, Luiz Eugenio Araujo de Moraes [UNIFESP], Silveira, Dartiu Xavier da [UNIFESP], Santos-Junior, Jair Guilherme [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/0013000000gv5
DOI: 10.1017/S1461145714000480
Texto Completo: http://dx.doi.org/10.1017/S1461145714000480
http://repositorio.unifesp.br/handle/11600/38355
Resumo: To address the role of mixed anxiety/mood disorder on appetitive associative learning, we verify whether previous chronic light deprivation changes ethanol-induced conditioned place preference and its respective expression of c-Fos and pCREB, markers of neuronal activity and plasticity. the experimental group was maintained in light deprivation for 24 h for a period of 4 wk. Subsequently, it was adapted to a standard light-dark cycle for 1 wk. As a control, some mice were maintained in standard cycle for a period of 4 wk (Naive group). Then, all animals were submitted to behavioral tests to assess emotionality: elevated plus maze; open field; and forced swim. After that, they were submitted to ethanol-induced conditioned place preference. Ninety minutes after the place preference test, they were perfused, and their brains processed for c-Fos and pCREB immunohistochemistry. Light deprivation induced anxiety-like trait (elevated plus maze), despair (forced swim), and hyperlocomotion (open field), common features seen in other animal models of depression. Ethanol-induced conditioned place preference was accompanied by increases on c-Fos and pCREB in the hippocampus, prefrontal cortex and striatum. Interestingly, mice previously submitted to light deprivation did not develop either acquisition and/or expression of ethanol-induced conditioned place preference or increases in c-Fos and pCREB. Therefore, chronic light deprivation mimics several behavioral aspects of other animal models of depression. Furthermore, it could be useful to study the neurochemical mechanisms involved in the dual diagnosis. However, given its likely deleterious effects on appetitive associative memory, it should be used with caution to investigate the cognitive aspects related to the dual diagnosis.
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spelling Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expressionAnxietyc-Fosconditioned place preferencedepressionlight deprivationpCREBTo address the role of mixed anxiety/mood disorder on appetitive associative learning, we verify whether previous chronic light deprivation changes ethanol-induced conditioned place preference and its respective expression of c-Fos and pCREB, markers of neuronal activity and plasticity. the experimental group was maintained in light deprivation for 24 h for a period of 4 wk. Subsequently, it was adapted to a standard light-dark cycle for 1 wk. As a control, some mice were maintained in standard cycle for a period of 4 wk (Naive group). Then, all animals were submitted to behavioral tests to assess emotionality: elevated plus maze; open field; and forced swim. After that, they were submitted to ethanol-induced conditioned place preference. Ninety minutes after the place preference test, they were perfused, and their brains processed for c-Fos and pCREB immunohistochemistry. Light deprivation induced anxiety-like trait (elevated plus maze), despair (forced swim), and hyperlocomotion (open field), common features seen in other animal models of depression. Ethanol-induced conditioned place preference was accompanied by increases on c-Fos and pCREB in the hippocampus, prefrontal cortex and striatum. Interestingly, mice previously submitted to light deprivation did not develop either acquisition and/or expression of ethanol-induced conditioned place preference or increases in c-Fos and pCREB. Therefore, chronic light deprivation mimics several behavioral aspects of other animal models of depression. Furthermore, it could be useful to study the neurochemical mechanisms involved in the dual diagnosis. However, given its likely deleterious effects on appetitive associative memory, it should be used with caution to investigate the cognitive aspects related to the dual diagnosis.Universidade Federal de São Paulo, Dept Psychiat, BR-04038001 São Paulo, BrazilUniversidade Federal de São Paulo, Neurobiol Lab, BR-04023062 São Paulo, BrazilFac Ciencias Med São Paulo, Dept Physiol Sci, BR-01221020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Psychiat, BR-04038001 São Paulo, BrazilUniversidade Federal de São Paulo, Neurobiol Lab, BR-04023062 São Paulo, BrazilWeb of ScienceCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Cambridge Univ PressUniversidade Federal de São Paulo (UNIFESP)Fac Ciencias Med São PauloVarela, Patricia [UNIFESP]Escosteguy-Neto, João Carlos [UNIFESP]Coelho, Carolina Tesone [UNIFESP]Mello, Luiz Eugenio Araujo de Moraes [UNIFESP]Silveira, Dartiu Xavier da [UNIFESP]Santos-Junior, Jair Guilherme [UNIFESP]2016-01-24T14:38:02Z2016-01-24T14:38:02Z2014-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1815-1830http://dx.doi.org/10.1017/S1461145714000480International Journal of Neuropsychopharmacology. New York: Cambridge Univ Press, v. 17, n. 11, p. 1815-1830, 2014.10.1017/S14611457140004801461-1457http://repositorio.unifesp.br/handle/11600/38355WOS:000345005400010ark:/48912/0013000000gv5engInternational Journal of Neuropsychopharmacologyinfo:eu-repo/semantics/openAccesshttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-02-18T12:05:29Zoai:repositorio.unifesp.br/:11600/38355Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T19:48:32.342472Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression
title Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression
spellingShingle Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression
Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression
Varela, Patricia [UNIFESP]
Anxiety
c-Fos
conditioned place preference
depression
light deprivation
pCREB
Varela, Patricia [UNIFESP]
Anxiety
c-Fos
conditioned place preference
depression
light deprivation
pCREB
title_short Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression
title_full Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression
title_fullStr Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression
Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression
title_full_unstemmed Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression
Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression
title_sort Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression
author Varela, Patricia [UNIFESP]
author_facet Varela, Patricia [UNIFESP]
Varela, Patricia [UNIFESP]
Escosteguy-Neto, João Carlos [UNIFESP]
Coelho, Carolina Tesone [UNIFESP]
Mello, Luiz Eugenio Araujo de Moraes [UNIFESP]
Silveira, Dartiu Xavier da [UNIFESP]
Santos-Junior, Jair Guilherme [UNIFESP]
Escosteguy-Neto, João Carlos [UNIFESP]
Coelho, Carolina Tesone [UNIFESP]
Mello, Luiz Eugenio Araujo de Moraes [UNIFESP]
Silveira, Dartiu Xavier da [UNIFESP]
Santos-Junior, Jair Guilherme [UNIFESP]
author_role author
author2 Escosteguy-Neto, João Carlos [UNIFESP]
Coelho, Carolina Tesone [UNIFESP]
Mello, Luiz Eugenio Araujo de Moraes [UNIFESP]
Silveira, Dartiu Xavier da [UNIFESP]
Santos-Junior, Jair Guilherme [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Fac Ciencias Med São Paulo
dc.contributor.author.fl_str_mv Varela, Patricia [UNIFESP]
Escosteguy-Neto, João Carlos [UNIFESP]
Coelho, Carolina Tesone [UNIFESP]
Mello, Luiz Eugenio Araujo de Moraes [UNIFESP]
Silveira, Dartiu Xavier da [UNIFESP]
Santos-Junior, Jair Guilherme [UNIFESP]
dc.subject.por.fl_str_mv Anxiety
c-Fos
conditioned place preference
depression
light deprivation
pCREB
topic Anxiety
c-Fos
conditioned place preference
depression
light deprivation
pCREB
description To address the role of mixed anxiety/mood disorder on appetitive associative learning, we verify whether previous chronic light deprivation changes ethanol-induced conditioned place preference and its respective expression of c-Fos and pCREB, markers of neuronal activity and plasticity. the experimental group was maintained in light deprivation for 24 h for a period of 4 wk. Subsequently, it was adapted to a standard light-dark cycle for 1 wk. As a control, some mice were maintained in standard cycle for a period of 4 wk (Naive group). Then, all animals were submitted to behavioral tests to assess emotionality: elevated plus maze; open field; and forced swim. After that, they were submitted to ethanol-induced conditioned place preference. Ninety minutes after the place preference test, they were perfused, and their brains processed for c-Fos and pCREB immunohistochemistry. Light deprivation induced anxiety-like trait (elevated plus maze), despair (forced swim), and hyperlocomotion (open field), common features seen in other animal models of depression. Ethanol-induced conditioned place preference was accompanied by increases on c-Fos and pCREB in the hippocampus, prefrontal cortex and striatum. Interestingly, mice previously submitted to light deprivation did not develop either acquisition and/or expression of ethanol-induced conditioned place preference or increases in c-Fos and pCREB. Therefore, chronic light deprivation mimics several behavioral aspects of other animal models of depression. Furthermore, it could be useful to study the neurochemical mechanisms involved in the dual diagnosis. However, given its likely deleterious effects on appetitive associative memory, it should be used with caution to investigate the cognitive aspects related to the dual diagnosis.
publishDate 2014
dc.date.none.fl_str_mv 2014-11-01
2016-01-24T14:38:02Z
2016-01-24T14:38:02Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1017/S1461145714000480
International Journal of Neuropsychopharmacology. New York: Cambridge Univ Press, v. 17, n. 11, p. 1815-1830, 2014.
10.1017/S1461145714000480
1461-1457
http://repositorio.unifesp.br/handle/11600/38355
WOS:000345005400010
dc.identifier.dark.fl_str_mv ark:/48912/0013000000gv5
url http://dx.doi.org/10.1017/S1461145714000480
http://repositorio.unifesp.br/handle/11600/38355
identifier_str_mv International Journal of Neuropsychopharmacology. New York: Cambridge Univ Press, v. 17, n. 11, p. 1815-1830, 2014.
10.1017/S1461145714000480
1461-1457
WOS:000345005400010
ark:/48912/0013000000gv5
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Neuropsychopharmacology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://journals.cambridge.org/action/displaySpecialPage?pageId=4676
eu_rights_str_mv openAccess
rights_invalid_str_mv http://journals.cambridge.org/action/displaySpecialPage?pageId=4676
dc.format.none.fl_str_mv 1815-1830
dc.publisher.none.fl_str_mv Cambridge Univ Press
publisher.none.fl_str_mv Cambridge Univ Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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dc.identifier.doi.none.fl_str_mv 10.1017/S1461145714000480