Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
dARK ID: | ark:/48912/0013000000gv5 |
DOI: | 10.1017/S1461145714000480 |
Texto Completo: | http://dx.doi.org/10.1017/S1461145714000480 http://repositorio.unifesp.br/handle/11600/38355 |
Resumo: | To address the role of mixed anxiety/mood disorder on appetitive associative learning, we verify whether previous chronic light deprivation changes ethanol-induced conditioned place preference and its respective expression of c-Fos and pCREB, markers of neuronal activity and plasticity. the experimental group was maintained in light deprivation for 24 h for a period of 4 wk. Subsequently, it was adapted to a standard light-dark cycle for 1 wk. As a control, some mice were maintained in standard cycle for a period of 4 wk (Naive group). Then, all animals were submitted to behavioral tests to assess emotionality: elevated plus maze; open field; and forced swim. After that, they were submitted to ethanol-induced conditioned place preference. Ninety minutes after the place preference test, they were perfused, and their brains processed for c-Fos and pCREB immunohistochemistry. Light deprivation induced anxiety-like trait (elevated plus maze), despair (forced swim), and hyperlocomotion (open field), common features seen in other animal models of depression. Ethanol-induced conditioned place preference was accompanied by increases on c-Fos and pCREB in the hippocampus, prefrontal cortex and striatum. Interestingly, mice previously submitted to light deprivation did not develop either acquisition and/or expression of ethanol-induced conditioned place preference or increases in c-Fos and pCREB. Therefore, chronic light deprivation mimics several behavioral aspects of other animal models of depression. Furthermore, it could be useful to study the neurochemical mechanisms involved in the dual diagnosis. However, given its likely deleterious effects on appetitive associative memory, it should be used with caution to investigate the cognitive aspects related to the dual diagnosis. |
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Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expressionAnxietyc-Fosconditioned place preferencedepressionlight deprivationpCREBTo address the role of mixed anxiety/mood disorder on appetitive associative learning, we verify whether previous chronic light deprivation changes ethanol-induced conditioned place preference and its respective expression of c-Fos and pCREB, markers of neuronal activity and plasticity. the experimental group was maintained in light deprivation for 24 h for a period of 4 wk. Subsequently, it was adapted to a standard light-dark cycle for 1 wk. As a control, some mice were maintained in standard cycle for a period of 4 wk (Naive group). Then, all animals were submitted to behavioral tests to assess emotionality: elevated plus maze; open field; and forced swim. After that, they were submitted to ethanol-induced conditioned place preference. Ninety minutes after the place preference test, they were perfused, and their brains processed for c-Fos and pCREB immunohistochemistry. Light deprivation induced anxiety-like trait (elevated plus maze), despair (forced swim), and hyperlocomotion (open field), common features seen in other animal models of depression. Ethanol-induced conditioned place preference was accompanied by increases on c-Fos and pCREB in the hippocampus, prefrontal cortex and striatum. Interestingly, mice previously submitted to light deprivation did not develop either acquisition and/or expression of ethanol-induced conditioned place preference or increases in c-Fos and pCREB. Therefore, chronic light deprivation mimics several behavioral aspects of other animal models of depression. Furthermore, it could be useful to study the neurochemical mechanisms involved in the dual diagnosis. However, given its likely deleterious effects on appetitive associative memory, it should be used with caution to investigate the cognitive aspects related to the dual diagnosis.Universidade Federal de São Paulo, Dept Psychiat, BR-04038001 São Paulo, BrazilUniversidade Federal de São Paulo, Neurobiol Lab, BR-04023062 São Paulo, BrazilFac Ciencias Med São Paulo, Dept Physiol Sci, BR-01221020 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Psychiat, BR-04038001 São Paulo, BrazilUniversidade Federal de São Paulo, Neurobiol Lab, BR-04023062 São Paulo, BrazilWeb of ScienceCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Cambridge Univ PressUniversidade Federal de São Paulo (UNIFESP)Fac Ciencias Med São PauloVarela, Patricia [UNIFESP]Escosteguy-Neto, João Carlos [UNIFESP]Coelho, Carolina Tesone [UNIFESP]Mello, Luiz Eugenio Araujo de Moraes [UNIFESP]Silveira, Dartiu Xavier da [UNIFESP]Santos-Junior, Jair Guilherme [UNIFESP]2016-01-24T14:38:02Z2016-01-24T14:38:02Z2014-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion1815-1830http://dx.doi.org/10.1017/S1461145714000480International Journal of Neuropsychopharmacology. New York: Cambridge Univ Press, v. 17, n. 11, p. 1815-1830, 2014.10.1017/S14611457140004801461-1457http://repositorio.unifesp.br/handle/11600/38355WOS:000345005400010ark:/48912/0013000000gv5engInternational Journal of Neuropsychopharmacologyinfo:eu-repo/semantics/openAccesshttp://journals.cambridge.org/action/displaySpecialPage?pageId=4676reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-02-18T12:05:29Zoai:repositorio.unifesp.br/:11600/38355Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T19:48:32.342472Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression |
title |
Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression |
spellingShingle |
Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression Varela, Patricia [UNIFESP] Anxiety c-Fos conditioned place preference depression light deprivation pCREB Varela, Patricia [UNIFESP] Anxiety c-Fos conditioned place preference depression light deprivation pCREB |
title_short |
Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression |
title_full |
Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression |
title_fullStr |
Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression |
title_full_unstemmed |
Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression |
title_sort |
Chronic light deprivation inhibits appetitive associative learning induced by ethanol and its respective c-Fos and pCREB expression |
author |
Varela, Patricia [UNIFESP] |
author_facet |
Varela, Patricia [UNIFESP] Varela, Patricia [UNIFESP] Escosteguy-Neto, João Carlos [UNIFESP] Coelho, Carolina Tesone [UNIFESP] Mello, Luiz Eugenio Araujo de Moraes [UNIFESP] Silveira, Dartiu Xavier da [UNIFESP] Santos-Junior, Jair Guilherme [UNIFESP] Escosteguy-Neto, João Carlos [UNIFESP] Coelho, Carolina Tesone [UNIFESP] Mello, Luiz Eugenio Araujo de Moraes [UNIFESP] Silveira, Dartiu Xavier da [UNIFESP] Santos-Junior, Jair Guilherme [UNIFESP] |
author_role |
author |
author2 |
Escosteguy-Neto, João Carlos [UNIFESP] Coelho, Carolina Tesone [UNIFESP] Mello, Luiz Eugenio Araujo de Moraes [UNIFESP] Silveira, Dartiu Xavier da [UNIFESP] Santos-Junior, Jair Guilherme [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Fac Ciencias Med São Paulo |
dc.contributor.author.fl_str_mv |
Varela, Patricia [UNIFESP] Escosteguy-Neto, João Carlos [UNIFESP] Coelho, Carolina Tesone [UNIFESP] Mello, Luiz Eugenio Araujo de Moraes [UNIFESP] Silveira, Dartiu Xavier da [UNIFESP] Santos-Junior, Jair Guilherme [UNIFESP] |
dc.subject.por.fl_str_mv |
Anxiety c-Fos conditioned place preference depression light deprivation pCREB |
topic |
Anxiety c-Fos conditioned place preference depression light deprivation pCREB |
description |
To address the role of mixed anxiety/mood disorder on appetitive associative learning, we verify whether previous chronic light deprivation changes ethanol-induced conditioned place preference and its respective expression of c-Fos and pCREB, markers of neuronal activity and plasticity. the experimental group was maintained in light deprivation for 24 h for a period of 4 wk. Subsequently, it was adapted to a standard light-dark cycle for 1 wk. As a control, some mice were maintained in standard cycle for a period of 4 wk (Naive group). Then, all animals were submitted to behavioral tests to assess emotionality: elevated plus maze; open field; and forced swim. After that, they were submitted to ethanol-induced conditioned place preference. Ninety minutes after the place preference test, they were perfused, and their brains processed for c-Fos and pCREB immunohistochemistry. Light deprivation induced anxiety-like trait (elevated plus maze), despair (forced swim), and hyperlocomotion (open field), common features seen in other animal models of depression. Ethanol-induced conditioned place preference was accompanied by increases on c-Fos and pCREB in the hippocampus, prefrontal cortex and striatum. Interestingly, mice previously submitted to light deprivation did not develop either acquisition and/or expression of ethanol-induced conditioned place preference or increases in c-Fos and pCREB. Therefore, chronic light deprivation mimics several behavioral aspects of other animal models of depression. Furthermore, it could be useful to study the neurochemical mechanisms involved in the dual diagnosis. However, given its likely deleterious effects on appetitive associative memory, it should be used with caution to investigate the cognitive aspects related to the dual diagnosis. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-11-01 2016-01-24T14:38:02Z 2016-01-24T14:38:02Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S1461145714000480 International Journal of Neuropsychopharmacology. New York: Cambridge Univ Press, v. 17, n. 11, p. 1815-1830, 2014. 10.1017/S1461145714000480 1461-1457 http://repositorio.unifesp.br/handle/11600/38355 WOS:000345005400010 |
dc.identifier.dark.fl_str_mv |
ark:/48912/0013000000gv5 |
url |
http://dx.doi.org/10.1017/S1461145714000480 http://repositorio.unifesp.br/handle/11600/38355 |
identifier_str_mv |
International Journal of Neuropsychopharmacology. New York: Cambridge Univ Press, v. 17, n. 11, p. 1815-1830, 2014. 10.1017/S1461145714000480 1461-1457 WOS:000345005400010 ark:/48912/0013000000gv5 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Neuropsychopharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://journals.cambridge.org/action/displaySpecialPage?pageId=4676 |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://journals.cambridge.org/action/displaySpecialPage?pageId=4676 |
dc.format.none.fl_str_mv |
1815-1830 |
dc.publisher.none.fl_str_mv |
Cambridge Univ Press |
publisher.none.fl_str_mv |
Cambridge Univ Press |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1822219253002010624 |
dc.identifier.doi.none.fl_str_mv |
10.1017/S1461145714000480 |