Mechanisms underlying the modulatory action of platelet activating factor (PAF) on the upregulation of kinin B-1 receptors in the rat paw

Detalhes bibliográficos
Autor(a) principal: Fernandes, Elizabeth S.
Data de Publicação: 2003
Outros Autores: Passos, Giselle F., Campos, Maria M., Araujo, José GVC, Pesquero, Jorge L., Avelllar, Maria Cristina Werneck [UNIFESP], Teixeira, Mauro M., Calixto, João B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1038/sj.bjp.0705314
http://repositorio.unifesp.br/handle/11600/27294
Resumo: 1 the present study evaluated the ability of the administration of platelet activating factor (PAF) to induce the upregulation of B-1 receptors in the rat paw.2 Local treatment with PAF resulted in a time-dependent increase of oedema formation induced by the B-1 receptor agonist des-Arg(9)-BK (des-Arg(9)-bradykinin), but not by the B-2 receptor agonist tyrosine 8-bradykinin. Functional upregulation of B-1 receptors was accompanied by a prominent increase of B-1 receptor mRNA expression in the rat paw.3 in PAF-treated paws, des-Arg(9)-BK-induced oedema formation was significantly inhibited by the B1 receptor antagonists des-Arg(9)-[Leu(8)]-BK and R-715. the effects of PAF pretreatment were receptor operated, as assessed by the effects of the PAF receptor antagonist WEB2086 or by desensitisation of PAF receptors.4 the protein synthesis inhibitor cycloheximide, the anti-inflammatory steroid dexamethasone or the nuclear factor (NF-kappaB) blockers pyrrolidine-dithiocarbamate ( PDTC) and Nalpha-tosyl-L-chloromethylketone significantly blocked the functional upregulation of B-1 receptors.5 the selectin inhibitor fucoidin, an anti-CD18 antibody or an anti-rat neutrophil antiserum, also significantly prevented des-Arg(9)-BK-induced paw oedema in rats pretreated with PAF.6 Intradermal injection of PAF induced a 25-fold increase of myeloperoxidase activity in the rat paw, a response that was significantly inhibited by fucoidin, anti-CD-18, anti-rat neutrophil antiserum or PDTC.7 Local treatment with PAF also resulted in a marked increase of NF-kappaB activation, an effect largely prevented by PDTC or by the anti-rat neutrophil antiserum.8 Collectively, the present results indicate that the induction of B-1 receptors following treatment with the chemotatic mediator PAF is dependent on the recruitment of neutrophils, an event that is under the control of adhesion molecules, protein synthesis and NF-kappaB activation. These findings provide new insights into the role played by cell migration and chemotatic factors on B-1 receptor upregulation in vivo.
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spelling Mechanisms underlying the modulatory action of platelet activating factor (PAF) on the upregulation of kinin B-1 receptors in the rat pawB-1 receptor expressionplatelet activating factoroedema formationneutrophil influxmyeloperoxidase activityNF-kappa B binding activation1 the present study evaluated the ability of the administration of platelet activating factor (PAF) to induce the upregulation of B-1 receptors in the rat paw.2 Local treatment with PAF resulted in a time-dependent increase of oedema formation induced by the B-1 receptor agonist des-Arg(9)-BK (des-Arg(9)-bradykinin), but not by the B-2 receptor agonist tyrosine 8-bradykinin. Functional upregulation of B-1 receptors was accompanied by a prominent increase of B-1 receptor mRNA expression in the rat paw.3 in PAF-treated paws, des-Arg(9)-BK-induced oedema formation was significantly inhibited by the B1 receptor antagonists des-Arg(9)-[Leu(8)]-BK and R-715. the effects of PAF pretreatment were receptor operated, as assessed by the effects of the PAF receptor antagonist WEB2086 or by desensitisation of PAF receptors.4 the protein synthesis inhibitor cycloheximide, the anti-inflammatory steroid dexamethasone or the nuclear factor (NF-kappaB) blockers pyrrolidine-dithiocarbamate ( PDTC) and Nalpha-tosyl-L-chloromethylketone significantly blocked the functional upregulation of B-1 receptors.5 the selectin inhibitor fucoidin, an anti-CD18 antibody or an anti-rat neutrophil antiserum, also significantly prevented des-Arg(9)-BK-induced paw oedema in rats pretreated with PAF.6 Intradermal injection of PAF induced a 25-fold increase of myeloperoxidase activity in the rat paw, a response that was significantly inhibited by fucoidin, anti-CD-18, anti-rat neutrophil antiserum or PDTC.7 Local treatment with PAF also resulted in a marked increase of NF-kappaB activation, an effect largely prevented by PDTC or by the anti-rat neutrophil antiserum.8 Collectively, the present results indicate that the induction of B-1 receptors following treatment with the chemotatic mediator PAF is dependent on the recruitment of neutrophils, an event that is under the control of adhesion molecules, protein synthesis and NF-kappaB activation. These findings provide new insights into the role played by cell migration and chemotatic factors on B-1 receptor upregulation in vivo.Univ Fed Santa Catarina, Ctr Biol Sci, Dept Pharmacol, BR-88015420 Florianopolis, SC, BrazilUniv Fed Minas Gerais, Dept Physiol & Biophys, Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, BR-04044020 São Paulo, BrazilUniv Fed Minas Gerais, Dept Biochem & Immunol, Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, BR-04044020 São Paulo, BrazilWeb of ScienceNature Publishing GroupUniversidade Federal de Santa Catarina (UFSC)Universidade Federal de Minas Gerais (UFMG)Universidade Federal de São Paulo (UNIFESP)Fernandes, Elizabeth S.Passos, Giselle F.Campos, Maria M.Araujo, José GVCPesquero, Jorge L.Avelllar, Maria Cristina Werneck [UNIFESP]Teixeira, Mauro M.Calixto, João B.2016-01-24T12:33:55Z2016-01-24T12:33:55Z2003-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion973-981http://dx.doi.org/10.1038/sj.bjp.0705314British Journal of Pharmacology. London: Nature Publishing Group, v. 139, n. 5, p. 973-981, 2003.10.1038/sj.bjp.07053140007-1188http://repositorio.unifesp.br/handle/11600/27294WOS:000184066800013engBritish Journal of Pharmacologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2016-01-24T10:33:55Zoai:repositorio.unifesp.br/:11600/27294Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652016-01-24T10:33:55Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Mechanisms underlying the modulatory action of platelet activating factor (PAF) on the upregulation of kinin B-1 receptors in the rat paw
title Mechanisms underlying the modulatory action of platelet activating factor (PAF) on the upregulation of kinin B-1 receptors in the rat paw
spellingShingle Mechanisms underlying the modulatory action of platelet activating factor (PAF) on the upregulation of kinin B-1 receptors in the rat paw
Fernandes, Elizabeth S.
B-1 receptor expression
platelet activating factor
oedema formation
neutrophil influx
myeloperoxidase activity
NF-kappa B binding activation
title_short Mechanisms underlying the modulatory action of platelet activating factor (PAF) on the upregulation of kinin B-1 receptors in the rat paw
title_full Mechanisms underlying the modulatory action of platelet activating factor (PAF) on the upregulation of kinin B-1 receptors in the rat paw
title_fullStr Mechanisms underlying the modulatory action of platelet activating factor (PAF) on the upregulation of kinin B-1 receptors in the rat paw
title_full_unstemmed Mechanisms underlying the modulatory action of platelet activating factor (PAF) on the upregulation of kinin B-1 receptors in the rat paw
title_sort Mechanisms underlying the modulatory action of platelet activating factor (PAF) on the upregulation of kinin B-1 receptors in the rat paw
author Fernandes, Elizabeth S.
author_facet Fernandes, Elizabeth S.
Passos, Giselle F.
Campos, Maria M.
Araujo, José GVC
Pesquero, Jorge L.
Avelllar, Maria Cristina Werneck [UNIFESP]
Teixeira, Mauro M.
Calixto, João B.
author_role author
author2 Passos, Giselle F.
Campos, Maria M.
Araujo, José GVC
Pesquero, Jorge L.
Avelllar, Maria Cristina Werneck [UNIFESP]
Teixeira, Mauro M.
Calixto, João B.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Santa Catarina (UFSC)
Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Fernandes, Elizabeth S.
Passos, Giselle F.
Campos, Maria M.
Araujo, José GVC
Pesquero, Jorge L.
Avelllar, Maria Cristina Werneck [UNIFESP]
Teixeira, Mauro M.
Calixto, João B.
dc.subject.por.fl_str_mv B-1 receptor expression
platelet activating factor
oedema formation
neutrophil influx
myeloperoxidase activity
NF-kappa B binding activation
topic B-1 receptor expression
platelet activating factor
oedema formation
neutrophil influx
myeloperoxidase activity
NF-kappa B binding activation
description 1 the present study evaluated the ability of the administration of platelet activating factor (PAF) to induce the upregulation of B-1 receptors in the rat paw.2 Local treatment with PAF resulted in a time-dependent increase of oedema formation induced by the B-1 receptor agonist des-Arg(9)-BK (des-Arg(9)-bradykinin), but not by the B-2 receptor agonist tyrosine 8-bradykinin. Functional upregulation of B-1 receptors was accompanied by a prominent increase of B-1 receptor mRNA expression in the rat paw.3 in PAF-treated paws, des-Arg(9)-BK-induced oedema formation was significantly inhibited by the B1 receptor antagonists des-Arg(9)-[Leu(8)]-BK and R-715. the effects of PAF pretreatment were receptor operated, as assessed by the effects of the PAF receptor antagonist WEB2086 or by desensitisation of PAF receptors.4 the protein synthesis inhibitor cycloheximide, the anti-inflammatory steroid dexamethasone or the nuclear factor (NF-kappaB) blockers pyrrolidine-dithiocarbamate ( PDTC) and Nalpha-tosyl-L-chloromethylketone significantly blocked the functional upregulation of B-1 receptors.5 the selectin inhibitor fucoidin, an anti-CD18 antibody or an anti-rat neutrophil antiserum, also significantly prevented des-Arg(9)-BK-induced paw oedema in rats pretreated with PAF.6 Intradermal injection of PAF induced a 25-fold increase of myeloperoxidase activity in the rat paw, a response that was significantly inhibited by fucoidin, anti-CD-18, anti-rat neutrophil antiserum or PDTC.7 Local treatment with PAF also resulted in a marked increase of NF-kappaB activation, an effect largely prevented by PDTC or by the anti-rat neutrophil antiserum.8 Collectively, the present results indicate that the induction of B-1 receptors following treatment with the chemotatic mediator PAF is dependent on the recruitment of neutrophils, an event that is under the control of adhesion molecules, protein synthesis and NF-kappaB activation. These findings provide new insights into the role played by cell migration and chemotatic factors on B-1 receptor upregulation in vivo.
publishDate 2003
dc.date.none.fl_str_mv 2003-07-01
2016-01-24T12:33:55Z
2016-01-24T12:33:55Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1038/sj.bjp.0705314
British Journal of Pharmacology. London: Nature Publishing Group, v. 139, n. 5, p. 973-981, 2003.
10.1038/sj.bjp.0705314
0007-1188
http://repositorio.unifesp.br/handle/11600/27294
WOS:000184066800013
url http://dx.doi.org/10.1038/sj.bjp.0705314
http://repositorio.unifesp.br/handle/11600/27294
identifier_str_mv British Journal of Pharmacology. London: Nature Publishing Group, v. 139, n. 5, p. 973-981, 2003.
10.1038/sj.bjp.0705314
0007-1188
WOS:000184066800013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv British Journal of Pharmacology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 973-981
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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