Parthenolide Modulates Immune Response in Cells from C57BL/6 Mice Induced with Experimental Autoimmune Encephalomyelitis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1055/s-0042-122783 https://repositorio.unifesp.br/handle/11600/54562 |
Resumo: | Multiple sclerosis is a chronic inflammatory and autoimmune disease of the central nervous system that affects more than 2.5 million people worldwide. Experimental autoimmune encephalomyelitis is a murine autoimmune disease used to study multiple sclerosis. Parthenolide, a natural sesguiterpene lactone found in Tanocetum parthenium L., is known for its strong anti-inflammatory activity. Herein, we have investigated the in vitro irnmunomodulatory effects of parthenolide on cytokine production and nitric oxide in cultured cells from myelin oligodendrocyte glycoprotein 35-55 amino acid peptide mice. Experimental autoimmune encephalomyelitis was induced in C576146 mice with myelin oligodendrocyte glycoprotein 35-55 amino acid peptide, and parthenolide was isolated from T. porthenium. Splenocytes and peritoneal cells were obtained from experimental autoimmune encephalomyelitis -induced mice and incubated with parthenolide (1, 5, and 20 mu M). After in vitro treatment with parthenolide, supernatants were collected, and nitric oxide and cytokines were measured. The results suggested that parthenolide may regulate the activity of Th17 and Th1 cells, mainly by decreasing IL 17, TNF-alpha, and interferon gamma production. This modulation may be related to the lower levels of IL-12p40 and IL-6 after treatment with parthenolide. It was shown, for the first time, that parthenolide presents in vitro immunomodulatory effects on inflammatory mediators produced by cells from experimental autoimmune encephalomyelitis-induced mice. |
id |
UFSP_07c28f8b007ee8cd4c46457bb24ee315 |
---|---|
oai_identifier_str |
oai:repositorio.unifesp.br/:11600/54562 |
network_acronym_str |
UFSP |
network_name_str |
Repositório Institucional da UNIFESP |
repository_id_str |
3465 |
spelling |
Parthenolide Modulates Immune Response in Cells from C57BL/6 Mice Induced with Experimental Autoimmune EncephalomyelitisparthenolideTanacetum partheniumAsteracraemultiple sclerosisexperimental autoimmune encephalomyelitisIL-17Multiple sclerosis is a chronic inflammatory and autoimmune disease of the central nervous system that affects more than 2.5 million people worldwide. Experimental autoimmune encephalomyelitis is a murine autoimmune disease used to study multiple sclerosis. Parthenolide, a natural sesguiterpene lactone found in Tanocetum parthenium L., is known for its strong anti-inflammatory activity. Herein, we have investigated the in vitro irnmunomodulatory effects of parthenolide on cytokine production and nitric oxide in cultured cells from myelin oligodendrocyte glycoprotein 35-55 amino acid peptide mice. Experimental autoimmune encephalomyelitis was induced in C576146 mice with myelin oligodendrocyte glycoprotein 35-55 amino acid peptide, and parthenolide was isolated from T. porthenium. Splenocytes and peritoneal cells were obtained from experimental autoimmune encephalomyelitis -induced mice and incubated with parthenolide (1, 5, and 20 mu M). After in vitro treatment with parthenolide, supernatants were collected, and nitric oxide and cytokines were measured. The results suggested that parthenolide may regulate the activity of Th17 and Th1 cells, mainly by decreasing IL 17, TNF-alpha, and interferon gamma production. This modulation may be related to the lower levels of IL-12p40 and IL-6 after treatment with parthenolide. It was shown, for the first time, that parthenolide presents in vitro immunomodulatory effects on inflammatory mediators produced by cells from experimental autoimmune encephalomyelitis-induced mice.Univ Fed Juiz de Fora, Fac Farm, Dept Ciencias Farmaceut, Rua Jose Lourenco Kelmer S-N, BR-36036900 Juiz De Fora, MG, BrazilUniv Fed Sao Paulo, Dept Biofis, Escola Paulista Med, Sao Paulo, BrazilUniv Fed Juiz de Fora, Dept Parasitol Microbiol & Imunol, Juiz De Fora, BrazilUniv Fed Sao Paulo, Dept Biofis, Escola Paulista Med, Sao Paulo, BrazilWeb of ScienceFAPEMIGCNPqCAPESPIBIC/CNPq/UFJFCNPqFAPEMIG: APQ 02015/14FAPEMIG: APQ 0171/11FAPEMIG: APQ 02140/14FAPEMIG: BPD 00284-14CNPq: 487 221/2012-5CNPq: 478 430/2013-2CNPq: 480 047/2013-8Georg Thieme Verlag Kg2020-07-13T11:53:21Z2020-07-13T11:53:21Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion693-700http://dx.doi.org/10.1055/s-0042-122783Planta Medica. Stuttgart, v. 83, n. 8, p. 693-700, 2017.10.1055/s-0042-1227830032-0943https://repositorio.unifesp.br/handle/11600/54562WOS:000403071800003engPlanta MedicaStuttgartinfo:eu-repo/semantics/openAccessAleixo de Carvalho, Lara SoaresAlmeida Fontes, Livia BeatrizGazolla, Matheus CoutinhoDias, Debora dos SantosJuliano, Maria Aparecida [UNIFESP]Macedo, Gilson Costado Amaral Correa, Jose OtavioDa Silva Filho, Ademar A.reponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-09-29T11:27:22Zoai:repositorio.unifesp.br/:11600/54562Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652021-09-29T11:27:22Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Parthenolide Modulates Immune Response in Cells from C57BL/6 Mice Induced with Experimental Autoimmune Encephalomyelitis |
title |
Parthenolide Modulates Immune Response in Cells from C57BL/6 Mice Induced with Experimental Autoimmune Encephalomyelitis |
spellingShingle |
Parthenolide Modulates Immune Response in Cells from C57BL/6 Mice Induced with Experimental Autoimmune Encephalomyelitis Aleixo de Carvalho, Lara Soares parthenolide Tanacetum parthenium Asteracrae multiple sclerosis experimental autoimmune encephalomyelitis IL-17 |
title_short |
Parthenolide Modulates Immune Response in Cells from C57BL/6 Mice Induced with Experimental Autoimmune Encephalomyelitis |
title_full |
Parthenolide Modulates Immune Response in Cells from C57BL/6 Mice Induced with Experimental Autoimmune Encephalomyelitis |
title_fullStr |
Parthenolide Modulates Immune Response in Cells from C57BL/6 Mice Induced with Experimental Autoimmune Encephalomyelitis |
title_full_unstemmed |
Parthenolide Modulates Immune Response in Cells from C57BL/6 Mice Induced with Experimental Autoimmune Encephalomyelitis |
title_sort |
Parthenolide Modulates Immune Response in Cells from C57BL/6 Mice Induced with Experimental Autoimmune Encephalomyelitis |
author |
Aleixo de Carvalho, Lara Soares |
author_facet |
Aleixo de Carvalho, Lara Soares Almeida Fontes, Livia Beatriz Gazolla, Matheus Coutinho Dias, Debora dos Santos Juliano, Maria Aparecida [UNIFESP] Macedo, Gilson Costa do Amaral Correa, Jose Otavio Da Silva Filho, Ademar A. |
author_role |
author |
author2 |
Almeida Fontes, Livia Beatriz Gazolla, Matheus Coutinho Dias, Debora dos Santos Juliano, Maria Aparecida [UNIFESP] Macedo, Gilson Costa do Amaral Correa, Jose Otavio Da Silva Filho, Ademar A. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Aleixo de Carvalho, Lara Soares Almeida Fontes, Livia Beatriz Gazolla, Matheus Coutinho Dias, Debora dos Santos Juliano, Maria Aparecida [UNIFESP] Macedo, Gilson Costa do Amaral Correa, Jose Otavio Da Silva Filho, Ademar A. |
dc.subject.por.fl_str_mv |
parthenolide Tanacetum parthenium Asteracrae multiple sclerosis experimental autoimmune encephalomyelitis IL-17 |
topic |
parthenolide Tanacetum parthenium Asteracrae multiple sclerosis experimental autoimmune encephalomyelitis IL-17 |
description |
Multiple sclerosis is a chronic inflammatory and autoimmune disease of the central nervous system that affects more than 2.5 million people worldwide. Experimental autoimmune encephalomyelitis is a murine autoimmune disease used to study multiple sclerosis. Parthenolide, a natural sesguiterpene lactone found in Tanocetum parthenium L., is known for its strong anti-inflammatory activity. Herein, we have investigated the in vitro irnmunomodulatory effects of parthenolide on cytokine production and nitric oxide in cultured cells from myelin oligodendrocyte glycoprotein 35-55 amino acid peptide mice. Experimental autoimmune encephalomyelitis was induced in C576146 mice with myelin oligodendrocyte glycoprotein 35-55 amino acid peptide, and parthenolide was isolated from T. porthenium. Splenocytes and peritoneal cells were obtained from experimental autoimmune encephalomyelitis -induced mice and incubated with parthenolide (1, 5, and 20 mu M). After in vitro treatment with parthenolide, supernatants were collected, and nitric oxide and cytokines were measured. The results suggested that parthenolide may regulate the activity of Th17 and Th1 cells, mainly by decreasing IL 17, TNF-alpha, and interferon gamma production. This modulation may be related to the lower levels of IL-12p40 and IL-6 after treatment with parthenolide. It was shown, for the first time, that parthenolide presents in vitro immunomodulatory effects on inflammatory mediators produced by cells from experimental autoimmune encephalomyelitis-induced mice. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2020-07-13T11:53:21Z 2020-07-13T11:53:21Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1055/s-0042-122783 Planta Medica. Stuttgart, v. 83, n. 8, p. 693-700, 2017. 10.1055/s-0042-122783 0032-0943 https://repositorio.unifesp.br/handle/11600/54562 WOS:000403071800003 |
url |
http://dx.doi.org/10.1055/s-0042-122783 https://repositorio.unifesp.br/handle/11600/54562 |
identifier_str_mv |
Planta Medica. Stuttgart, v. 83, n. 8, p. 693-700, 2017. 10.1055/s-0042-122783 0032-0943 WOS:000403071800003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Planta Medica |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
693-700 |
dc.coverage.none.fl_str_mv |
Stuttgart |
dc.publisher.none.fl_str_mv |
Georg Thieme Verlag Kg |
publisher.none.fl_str_mv |
Georg Thieme Verlag Kg |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268332496388096 |