Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-879X2002000100003 http://repositorio.unifesp.br/handle/11600/1309 |
Resumo: | Renin is an enzyme involved in the stepwise generation of angiotensin II. Juxtaglomerular cells are the main source of plasma renin, but renin activity has been detected in other cell types. In the present study we evaluated the presence of renin mRNA in adult male Wistar rat and mouse (C-57 Black/6) mesangial cells (MC) and their ability to process, store and release both the active and inactive forms of the enzyme. Active renin and total renin content obtained after trypsin treatment were estimated by angiotensinogen consumption analyzed by SDS-PAGE electrophoresis and quantified by angiotensin I generation by HPLC. Renin mRNA, detected by RT-PCR, was present in both rat and mouse MC under basal conditions. Active renin was significantly higher (P<0.05) in the cell lysate (43.5 ± 5.7 ng h-1 10(6) cells) than in the culture medium (12.5 ± 2.5 ng h-1 10(6) cells). Inactive prorenin content was similar for the intra- and extracellular compartments (9.7 ± 3.1 and 3.9 ± 0.9 ng h-1 10(6) cells). Free active renin was the predominant form found in both cell compartments. These results indicate that MC in culture are able to synthesize and translate renin mRNA probably as inactive prorenin which is mostly processed to active renin inside the cell. MC secrete both forms of the enzyme but at a lower level compared with intracellular content, suggesting that the main role of renin synthesized by MC may be the intracellular generation of angiotensin II. |
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Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cellsReninProreninMesangial cellKidneyRenin is an enzyme involved in the stepwise generation of angiotensin II. Juxtaglomerular cells are the main source of plasma renin, but renin activity has been detected in other cell types. In the present study we evaluated the presence of renin mRNA in adult male Wistar rat and mouse (C-57 Black/6) mesangial cells (MC) and their ability to process, store and release both the active and inactive forms of the enzyme. Active renin and total renin content obtained after trypsin treatment were estimated by angiotensinogen consumption analyzed by SDS-PAGE electrophoresis and quantified by angiotensin I generation by HPLC. Renin mRNA, detected by RT-PCR, was present in both rat and mouse MC under basal conditions. Active renin was significantly higher (P<0.05) in the cell lysate (43.5 ± 5.7 ng h-1 10(6) cells) than in the culture medium (12.5 ± 2.5 ng h-1 10(6) cells). Inactive prorenin content was similar for the intra- and extracellular compartments (9.7 ± 3.1 and 3.9 ± 0.9 ng h-1 10(6) cells). Free active renin was the predominant form found in both cell compartments. These results indicate that MC in culture are able to synthesize and translate renin mRNA probably as inactive prorenin which is mostly processed to active renin inside the cell. MC secrete both forms of the enzyme but at a lower level compared with intracellular content, suggesting that the main role of renin synthesized by MC may be the intracellular generation of angiotensin II.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Disciplina de NefrologiaUNIFESP, EPM, Disciplina de NefrologiaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal de São Paulo (UNIFESP)Andrade, A.q. [UNIFESP]Casarini, Dulce Elena [UNIFESP]Schor, Nestor [UNIFESP]Boim, Mirian Aparecida [UNIFESP]2015-06-14T13:29:34Z2015-06-14T13:29:34Z2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion17-24application/pdfhttp://dx.doi.org/10.1590/S0100-879X2002000100003Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 1, p. 17-24, 2002.10.1590/S0100-879X2002000100003S0100-879X2002000100003.pdf0100-879XS0100-879X2002000100003http://repositorio.unifesp.br/handle/11600/1309WOS:000173942600003engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T15:17:34Zoai:repositorio.unifesp.br/:11600/1309Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T15:17:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells |
title |
Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells |
spellingShingle |
Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells Andrade, A.q. [UNIFESP] Renin Prorenin Mesangial cell Kidney |
title_short |
Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells |
title_full |
Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells |
title_fullStr |
Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells |
title_full_unstemmed |
Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells |
title_sort |
Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells |
author |
Andrade, A.q. [UNIFESP] |
author_facet |
Andrade, A.q. [UNIFESP] Casarini, Dulce Elena [UNIFESP] Schor, Nestor [UNIFESP] Boim, Mirian Aparecida [UNIFESP] |
author_role |
author |
author2 |
Casarini, Dulce Elena [UNIFESP] Schor, Nestor [UNIFESP] Boim, Mirian Aparecida [UNIFESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Andrade, A.q. [UNIFESP] Casarini, Dulce Elena [UNIFESP] Schor, Nestor [UNIFESP] Boim, Mirian Aparecida [UNIFESP] |
dc.subject.por.fl_str_mv |
Renin Prorenin Mesangial cell Kidney |
topic |
Renin Prorenin Mesangial cell Kidney |
description |
Renin is an enzyme involved in the stepwise generation of angiotensin II. Juxtaglomerular cells are the main source of plasma renin, but renin activity has been detected in other cell types. In the present study we evaluated the presence of renin mRNA in adult male Wistar rat and mouse (C-57 Black/6) mesangial cells (MC) and their ability to process, store and release both the active and inactive forms of the enzyme. Active renin and total renin content obtained after trypsin treatment were estimated by angiotensinogen consumption analyzed by SDS-PAGE electrophoresis and quantified by angiotensin I generation by HPLC. Renin mRNA, detected by RT-PCR, was present in both rat and mouse MC under basal conditions. Active renin was significantly higher (P<0.05) in the cell lysate (43.5 ± 5.7 ng h-1 10(6) cells) than in the culture medium (12.5 ± 2.5 ng h-1 10(6) cells). Inactive prorenin content was similar for the intra- and extracellular compartments (9.7 ± 3.1 and 3.9 ± 0.9 ng h-1 10(6) cells). Free active renin was the predominant form found in both cell compartments. These results indicate that MC in culture are able to synthesize and translate renin mRNA probably as inactive prorenin which is mostly processed to active renin inside the cell. MC secrete both forms of the enzyme but at a lower level compared with intracellular content, suggesting that the main role of renin synthesized by MC may be the intracellular generation of angiotensin II. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-01-01 2015-06-14T13:29:34Z 2015-06-14T13:29:34Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X2002000100003 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 1, p. 17-24, 2002. 10.1590/S0100-879X2002000100003 S0100-879X2002000100003.pdf 0100-879X S0100-879X2002000100003 http://repositorio.unifesp.br/handle/11600/1309 WOS:000173942600003 |
url |
http://dx.doi.org/10.1590/S0100-879X2002000100003 http://repositorio.unifesp.br/handle/11600/1309 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 1, p. 17-24, 2002. 10.1590/S0100-879X2002000100003 S0100-879X2002000100003.pdf 0100-879X S0100-879X2002000100003 WOS:000173942600003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17-24 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
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1814268444857597952 |