Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells

Detalhes bibliográficos
Autor(a) principal: Andrade, A.q. [UNIFESP]
Data de Publicação: 2002
Outros Autores: Casarini, Dulce Elena [UNIFESP], Schor, Nestor [UNIFESP], Boim, Mirian Aparecida [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2002000100003
http://repositorio.unifesp.br/handle/11600/1309
Resumo: Renin is an enzyme involved in the stepwise generation of angiotensin II. Juxtaglomerular cells are the main source of plasma renin, but renin activity has been detected in other cell types. In the present study we evaluated the presence of renin mRNA in adult male Wistar rat and mouse (C-57 Black/6) mesangial cells (MC) and their ability to process, store and release both the active and inactive forms of the enzyme. Active renin and total renin content obtained after trypsin treatment were estimated by angiotensinogen consumption analyzed by SDS-PAGE electrophoresis and quantified by angiotensin I generation by HPLC. Renin mRNA, detected by RT-PCR, was present in both rat and mouse MC under basal conditions. Active renin was significantly higher (P<0.05) in the cell lysate (43.5 ± 5.7 ng h-1 10(6) cells) than in the culture medium (12.5 ± 2.5 ng h-1 10(6) cells). Inactive prorenin content was similar for the intra- and extracellular compartments (9.7 ± 3.1 and 3.9 ± 0.9 ng h-1 10(6) cells). Free active renin was the predominant form found in both cell compartments. These results indicate that MC in culture are able to synthesize and translate renin mRNA probably as inactive prorenin which is mostly processed to active renin inside the cell. MC secrete both forms of the enzyme but at a lower level compared with intracellular content, suggesting that the main role of renin synthesized by MC may be the intracellular generation of angiotensin II.
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spelling Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cellsReninProreninMesangial cellKidneyRenin is an enzyme involved in the stepwise generation of angiotensin II. Juxtaglomerular cells are the main source of plasma renin, but renin activity has been detected in other cell types. In the present study we evaluated the presence of renin mRNA in adult male Wistar rat and mouse (C-57 Black/6) mesangial cells (MC) and their ability to process, store and release both the active and inactive forms of the enzyme. Active renin and total renin content obtained after trypsin treatment were estimated by angiotensinogen consumption analyzed by SDS-PAGE electrophoresis and quantified by angiotensin I generation by HPLC. Renin mRNA, detected by RT-PCR, was present in both rat and mouse MC under basal conditions. Active renin was significantly higher (P<0.05) in the cell lysate (43.5 ± 5.7 ng h-1 10(6) cells) than in the culture medium (12.5 ± 2.5 ng h-1 10(6) cells). Inactive prorenin content was similar for the intra- and extracellular compartments (9.7 ± 3.1 and 3.9 ± 0.9 ng h-1 10(6) cells). Free active renin was the predominant form found in both cell compartments. These results indicate that MC in culture are able to synthesize and translate renin mRNA probably as inactive prorenin which is mostly processed to active renin inside the cell. MC secrete both forms of the enzyme but at a lower level compared with intracellular content, suggesting that the main role of renin synthesized by MC may be the intracellular generation of angiotensin II.Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Disciplina de NefrologiaUNIFESP, EPM, Disciplina de NefrologiaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Federal de São Paulo (UNIFESP)Andrade, A.q. [UNIFESP]Casarini, Dulce Elena [UNIFESP]Schor, Nestor [UNIFESP]Boim, Mirian Aparecida [UNIFESP]2015-06-14T13:29:34Z2015-06-14T13:29:34Z2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion17-24application/pdfhttp://dx.doi.org/10.1590/S0100-879X2002000100003Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 1, p. 17-24, 2002.10.1590/S0100-879X2002000100003S0100-879X2002000100003.pdf0100-879XS0100-879X2002000100003http://repositorio.unifesp.br/handle/11600/1309WOS:000173942600003engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T15:17:34Zoai:repositorio.unifesp.br/:11600/1309Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T15:17:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells
title Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells
spellingShingle Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells
Andrade, A.q. [UNIFESP]
Renin
Prorenin
Mesangial cell
Kidney
title_short Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells
title_full Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells
title_fullStr Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells
title_full_unstemmed Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells
title_sort Characterization of renin mRNA expression and enzyme activity in rat and mouse mesangial cells
author Andrade, A.q. [UNIFESP]
author_facet Andrade, A.q. [UNIFESP]
Casarini, Dulce Elena [UNIFESP]
Schor, Nestor [UNIFESP]
Boim, Mirian Aparecida [UNIFESP]
author_role author
author2 Casarini, Dulce Elena [UNIFESP]
Schor, Nestor [UNIFESP]
Boim, Mirian Aparecida [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Andrade, A.q. [UNIFESP]
Casarini, Dulce Elena [UNIFESP]
Schor, Nestor [UNIFESP]
Boim, Mirian Aparecida [UNIFESP]
dc.subject.por.fl_str_mv Renin
Prorenin
Mesangial cell
Kidney
topic Renin
Prorenin
Mesangial cell
Kidney
description Renin is an enzyme involved in the stepwise generation of angiotensin II. Juxtaglomerular cells are the main source of plasma renin, but renin activity has been detected in other cell types. In the present study we evaluated the presence of renin mRNA in adult male Wistar rat and mouse (C-57 Black/6) mesangial cells (MC) and their ability to process, store and release both the active and inactive forms of the enzyme. Active renin and total renin content obtained after trypsin treatment were estimated by angiotensinogen consumption analyzed by SDS-PAGE electrophoresis and quantified by angiotensin I generation by HPLC. Renin mRNA, detected by RT-PCR, was present in both rat and mouse MC under basal conditions. Active renin was significantly higher (P<0.05) in the cell lysate (43.5 ± 5.7 ng h-1 10(6) cells) than in the culture medium (12.5 ± 2.5 ng h-1 10(6) cells). Inactive prorenin content was similar for the intra- and extracellular compartments (9.7 ± 3.1 and 3.9 ± 0.9 ng h-1 10(6) cells). Free active renin was the predominant form found in both cell compartments. These results indicate that MC in culture are able to synthesize and translate renin mRNA probably as inactive prorenin which is mostly processed to active renin inside the cell. MC secrete both forms of the enzyme but at a lower level compared with intracellular content, suggesting that the main role of renin synthesized by MC may be the intracellular generation of angiotensin II.
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
2015-06-14T13:29:34Z
2015-06-14T13:29:34Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2002000100003
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 1, p. 17-24, 2002.
10.1590/S0100-879X2002000100003
S0100-879X2002000100003.pdf
0100-879X
S0100-879X2002000100003
http://repositorio.unifesp.br/handle/11600/1309
WOS:000173942600003
url http://dx.doi.org/10.1590/S0100-879X2002000100003
http://repositorio.unifesp.br/handle/11600/1309
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 35, n. 1, p. 17-24, 2002.
10.1590/S0100-879X2002000100003
S0100-879X2002000100003.pdf
0100-879X
S0100-879X2002000100003
WOS:000173942600003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17-24
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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