Evaluation of MCT1, MCT4 and CD147 Genes in Peripheral Blood Cells of Breast Cancer Patients and Their Potential Use as Diagnostic and Prognostic Markers

• Autor(a) principal: Luz, Maria Claudia de B.
• Data de Publicação: 2017
• Outros Autores: Perez, Matheus M., Azzalis, Ligia A. [UNIFESP], Sousa, Luiz Vinicius de A., Adami, Fernando, Fonseca, Fernando L. A. [UNIFESP], Alves, Beatriz da C. A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.3390/ijms18040170; https://repositorio.unifesp.br/handle/11600/54789
• topic: biomarkers; prognosis; metastasis; breast cancer; monocarboxylate transporter (MCT); hypoxia

Background: Patients with breast cancer-the deadliest cancer among women-are at constant risk of developing metastasis. Oxidative stress and hypoxia are common feature of tumor cells that can proliferate even in a resultant metabolic acidosis. Despite the low extracellular pH, intracellular pH of tumor cells remains relatively normal, or even more alkaline due to the action of a membrane protein family known as monocarboxylate transporters (MCTs). The objective of this study was to verify the diagnostic and prognostic value of MCT1, MCT4 and CD147 in tumor and peripheral blood samples of patients with breast cancer undergoing chemotherapic treatment. Methods: Differential expression of MCT1, MCT4 and CD147 obtained by qPCR was determined by 2(-Delta Delta Cq) method between biological samples (tumor and serial samples of peripheral) of patients (n = 125) and healthy women (n = 25). Results: tumor samples with higher histological grades have shown higher expression of these markers