Avaliação morfológica, imunoistoquímica e molecular da musculatura de uretra de ratas após trauma induzido e estimulação elétrica de assoalho pélvico

• Autor(a) principal: Franco, Gisela Rosa [UNIFESP]
• Data de Publicação: 2016
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=3633001; https://repositorio.unifesp.br/handle/11600/47047
• topic: incontinence; uretra; vagina; therapy electrical stimulation; incontinência urinária; uretra; vagina; terapia por estimulação elétrica

Introduction: damage to the urethral component with a consequent reduction of pressure intraurethral, ??they are associated with the pathophysiology of urinary incontinence. The trauma pelvic floor induced in animals causes hypoxia, ischemia and breakdown Structural the urethral tissues. Electrical stimulation is a physical therapy modality capable of increasing intraurethral pressure, whereby their efficacy in treatment of urinary incontinence. However, modifications and immunohistochemical molecular the urethra resulting from the trauma of the pelvic floor for 12 hours and the effect of electric current used in the damaged tissue recovery, are still unknown. Thus, we set out to evaluate the effects of trauma Pelvic floor in the urethra components of rats at different times after trauma and the effects of electric current in rats of urethral components applied after trauma. Methods: The ureters were taken from rats 5 groups: 1) control without trauma; two) with induced trauma to sacrifice the rats and extraction of the ureters after 1 hour trauma; 3) with induced trauma to sacrifice the rats and after extraction of the ureters 7 days of injury; 4) with induced trauma to sacrifice the rats and extraction of ureters after 30 days of trauma; 5) with induced trauma, treatment electrostimulation, sacrifice the rats and extraction of the ureters after 30 days of trauma. The pelvic floor trauma model occurred through vaginal dilation (DV) intermittent maintained for 12 hours. The electrical stimulation treatment was performed by transvaginal probe, totaling 12 alternate sessions for 30 days. At Samples of the ureters were processed and analyzed as in (1) and morphology structure of connective tissue, nerve and vascular, through analysis Histological and immunohistochemical; (2) expression of genes and proteins involved in metabolism urethral components through R T qPCR and Western Blotting respectively. Results: One hour after the DV histological analysis revealed consistent urethra tissue disorganization. In the same period we observed a significant increase in expression of genes VEGF, NGF and COL1A1 Mki67 (p = 0.004, p = 0.009 and p = 0.03, respectively). Seven days after the DV expression of the COL1A1 gene remained increased (p = 0.009), and expression of COL3A1 gene happened to have increased in the control group (p = 0.009). The immunoreactivity of COL3 also increased in the control group (p = 0.03). The group 30 days after the trauma DV continued to show increased immunoreactivity of COL3A1 (p = 0.02) in the control group. The group that received treatment with electrostimulation after DV showed an increase in COL1A1 gene expression in relation to the group 30 days after the trauma (p = 0.036). Conclusion: we identify acute changes and persistent changes in the profile genetic, morphological and immunohistochemical in the urethral tissue components with trauma model for 12h intermittent, which show a recovery in tissue 30 days after injury. We observed that treatment with electrostimulation not improved the recovery of components of the extracellular matrix, as well as growth factors VEGF and NGF.