Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| dARK ID: | ark:/48912/001300000661w |
| Texto Completo: | http://dx.doi.org/10.1128/JCM.43.10.5065-5068.2005 http://repositorio.unifesp.br/handle/11600/28480 |
| Resumo: | In this study we compared the performance of the Chagas Stat-Pak rapid immunochromatographic test with a standard enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of Chagas' disease in Central America. Out of 3,400 blood donor samples, 156 (4.6%) were positive in both assays. Three sera out of 2,084 samples from reference laboratories were negative with the rapid test but positive with the ELISA (99.8% agreement). Agreement of 100% between the two tests was observed with 339 additional sera from patients with cardiopathies and 175 sera from potential blood donors in emergency surgical cases occurring on weekends or at night. in conclusion, Chagas Stat-Pak showed 99.6% and 99.9% sensitivity and specificity, respectively, when assayed with 5,998 serum samples. It is a sensitive and specific alternative to the ELISA, as required in medical emergencies and blood screenings in Central America. |
| id |
UFSP_0994c70af03d19cfcdb0b31e93fa1c5e |
|---|---|
| oai_identifier_str |
oai:repositorio.unifesp.br/:11600/28480 |
| network_acronym_str |
UFSP |
| network_name_str |
Repositório Institucional da UNIFESP |
| repository_id_str |
3465 |
| spelling |
Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central AmericaIn this study we compared the performance of the Chagas Stat-Pak rapid immunochromatographic test with a standard enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of Chagas' disease in Central America. Out of 3,400 blood donor samples, 156 (4.6%) were positive in both assays. Three sera out of 2,084 samples from reference laboratories were negative with the rapid test but positive with the ELISA (99.8% agreement). Agreement of 100% between the two tests was observed with 339 additional sera from patients with cardiopathies and 175 sera from potential blood donors in emergency surgical cases occurring on weekends or at night. in conclusion, Chagas Stat-Pak showed 99.6% and 99.9% sensitivity and specificity, respectively, when assayed with 5,998 serum samples. It is a sensitive and specific alternative to the ELISA, as required in medical emergencies and blood screenings in Central America.Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilSecretaria Salud, Lab Cent Referencia Enfermedad Chagas & Leishmani, Tegucigalpa, HondurasCruz Roja Hondurena, Tegucigalpa, HondurasMinist Salud, Ctr Nacl Diagnost & Referencia, Managua, NicaraguaMinist Salud Publ & Asistencia Social, Cent Lab, San Salvador, El SalvadorCSIC, Inst Parasitol & Biomed, Granada, SpainUniv São Paulo, Inst Quim, Dept Bioquim, BR-01498 São Paulo, BrazilUniv Simon Bolivar, Dept Biol Celular, Caracas, VenezuelaInst Invest Engn Genet & Biol Mol, INGEBI, Buenos Aires, DF, ArgentinaChembio Diagnost Syst Inc, Medford, NY USAUniv São Paulo, Inst Med Trop São Paulo, São Paulo, BrazilUniv Fed Goias, Inst Patol Trop & Saude Publ, Goiania, Go, BrazilUniv Fed Goias, Fac Med, Goiania, Go, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, BrazilWeb of ScienceAmer Soc MicrobiologyUniversidade Federal de São Paulo (UNIFESP)Secretaria SaludCruz Roja HondurenaMinist SaludMinist Salud Publ & Asistencia SocialCSICUniversidade de São Paulo (USP)Univ Simon BolivarInst Invest Engn Genet & Biol MolChembio Diagnost Syst IncUniversidade Federal de Goiás (UFG)Ponce, CarlosPonce, ElisaVinelli, ElizabethMontoya, AlbertoAguilar, Vilma deGonzalez, AntonioZingales, BiancaRangel-Aldao, RafaelLevin, Mariano J.Esfandiari, JavanUmezawa, Eufrosina S.Luquetti, Alejandro O.Silveira, Jose Franco da [UNIFESP]2016-01-24T12:38:04Z2016-01-24T12:38:04Z2005-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion5065-5068application/pdfhttp://dx.doi.org/10.1128/JCM.43.10.5065-5068.2005Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 10, p. 5065-5068, 2005.10.1128/JCM.43.10.5065-5068.2005WOS000232762500020.pdf0095-1137http://repositorio.unifesp.br/handle/11600/28480WOS:000232762500020ark:/48912/001300000661wengJournal of Clinical Microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-30T19:25:47Zoai:repositorio.unifesp.br/:11600/28480Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-30T19:25:47Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America |
| title |
Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America |
| spellingShingle |
Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America Ponce, Carlos |
| title_short |
Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America |
| title_full |
Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America |
| title_fullStr |
Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America |
| title_full_unstemmed |
Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America |
| title_sort |
Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America |
| author |
Ponce, Carlos |
| author_facet |
Ponce, Carlos Ponce, Elisa Vinelli, Elizabeth Montoya, Alberto Aguilar, Vilma de Gonzalez, Antonio Zingales, Bianca Rangel-Aldao, Rafael Levin, Mariano J. Esfandiari, Javan Umezawa, Eufrosina S. Luquetti, Alejandro O. Silveira, Jose Franco da [UNIFESP] |
| author_role |
author |
| author2 |
Ponce, Elisa Vinelli, Elizabeth Montoya, Alberto Aguilar, Vilma de Gonzalez, Antonio Zingales, Bianca Rangel-Aldao, Rafael Levin, Mariano J. Esfandiari, Javan Umezawa, Eufrosina S. Luquetti, Alejandro O. Silveira, Jose Franco da [UNIFESP] |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Secretaria Salud Cruz Roja Hondurena Minist Salud Minist Salud Publ & Asistencia Social CSIC Universidade de São Paulo (USP) Univ Simon Bolivar Inst Invest Engn Genet & Biol Mol Chembio Diagnost Syst Inc Universidade Federal de Goiás (UFG) |
| dc.contributor.author.fl_str_mv |
Ponce, Carlos Ponce, Elisa Vinelli, Elizabeth Montoya, Alberto Aguilar, Vilma de Gonzalez, Antonio Zingales, Bianca Rangel-Aldao, Rafael Levin, Mariano J. Esfandiari, Javan Umezawa, Eufrosina S. Luquetti, Alejandro O. Silveira, Jose Franco da [UNIFESP] |
| description |
In this study we compared the performance of the Chagas Stat-Pak rapid immunochromatographic test with a standard enzyme-linked immunosorbent assay (ELISA) in the serodiagnosis of Chagas' disease in Central America. Out of 3,400 blood donor samples, 156 (4.6%) were positive in both assays. Three sera out of 2,084 samples from reference laboratories were negative with the rapid test but positive with the ELISA (99.8% agreement). Agreement of 100% between the two tests was observed with 339 additional sera from patients with cardiopathies and 175 sera from potential blood donors in emergency surgical cases occurring on weekends or at night. in conclusion, Chagas Stat-Pak showed 99.6% and 99.9% sensitivity and specificity, respectively, when assayed with 5,998 serum samples. It is a sensitive and specific alternative to the ELISA, as required in medical emergencies and blood screenings in Central America. |
| publishDate |
2005 |
| dc.date.none.fl_str_mv |
2005-10-01 2016-01-24T12:38:04Z 2016-01-24T12:38:04Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1128/JCM.43.10.5065-5068.2005 Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 10, p. 5065-5068, 2005. 10.1128/JCM.43.10.5065-5068.2005 WOS000232762500020.pdf 0095-1137 http://repositorio.unifesp.br/handle/11600/28480 WOS:000232762500020 |
| dc.identifier.dark.fl_str_mv |
ark:/48912/001300000661w |
| url |
http://dx.doi.org/10.1128/JCM.43.10.5065-5068.2005 http://repositorio.unifesp.br/handle/11600/28480 |
| identifier_str_mv |
Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 10, p. 5065-5068, 2005. 10.1128/JCM.43.10.5065-5068.2005 WOS000232762500020.pdf 0095-1137 WOS:000232762500020 ark:/48912/001300000661w |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Journal of Clinical Microbiology |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
5065-5068 application/pdf |
| dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
| publisher.none.fl_str_mv |
Amer Soc Microbiology |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
| collection |
Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
| _version_ |
1828484607594463232 |
