Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/37872 http://dx.doi.org/10.1186/1475-2875-13-230 |
Resumo: | Background: the mechanisms through which infection with Plasmodium spp. result in lung disease are largely unknown. Recently a number of mouse models have been developed to research malaria-associated lung injury but no detailed ultrastructure studies of the disease in its terminal stages in a murine model have yet been published. the goal was to perform an ultrastructural analysis of the lungs of mice that died with malaria-associated acute lung injury/acute respiratory distress syndrome to better determine the relevancy of the murine models and investigate the mechanism of disease.Methods: DBA/2 mice were infected with Plasmodium berghei strain ANKA. Mice had their lungs removed immediately after death, processed using standard methods and viewed by transmission electron microscopy (TEM).Results: Infected red blood cell: endothelium contact, swollen endothelium with distended cytoplasmic extensions and thickening of endothelium basement membrane were observed. Septa were thick and filled with congested capillaries and leukocytes and the alveolar spaces contained blood cells, oedema and cell debris.Conclusion: Results show that the lung ultrastructure of P. berghei ANKA-infected mice has similar features to what has been described in post-mortem TEM studies of lungs from individuals infected with Plasmodium falciparum. These data support the use of murine models to study malaria-associated acute lung injury. |
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Aitken, Elizabeth H.Negri, Elnara M.Barboza, Renato [UNIFESP]Lima, Maria RiAlvarez, Jose M.Marinho, Claudio R. F.Caldini, Elia G.Epiphanio, SabrinaUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:37:27Z2016-01-24T14:37:27Z2014-06-13Malaria Journal. London: Biomed Central Ltd, v. 13, 10 p., 2014.1475-2875http://repositorio.unifesp.br/handle/11600/37872http://dx.doi.org/10.1186/1475-2875-13-230WOS000338889000001.pdf10.1186/1475-2875-13-230WOS:000338889000001Background: the mechanisms through which infection with Plasmodium spp. result in lung disease are largely unknown. Recently a number of mouse models have been developed to research malaria-associated lung injury but no detailed ultrastructure studies of the disease in its terminal stages in a murine model have yet been published. the goal was to perform an ultrastructural analysis of the lungs of mice that died with malaria-associated acute lung injury/acute respiratory distress syndrome to better determine the relevancy of the murine models and investigate the mechanism of disease.Methods: DBA/2 mice were infected with Plasmodium berghei strain ANKA. Mice had their lungs removed immediately after death, processed using standard methods and viewed by transmission electron microscopy (TEM).Results: Infected red blood cell: endothelium contact, swollen endothelium with distended cytoplasmic extensions and thickening of endothelium basement membrane were observed. Septa were thick and filled with congested capillaries and leukocytes and the alveolar spaces contained blood cells, oedema and cell debris.Conclusion: Results show that the lung ultrastructure of P. berghei ANKA-infected mice has similar features to what has been described in post-mortem TEM studies of lungs from individuals infected with Plasmodium falciparum. These data support the use of murine models to study malaria-associated acute lung injury.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ São Paulo, Dept Immunol, São Paulo, BrazilUniv São Paulo, Fac Med, Lab Med Invest 59, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Phys & Earth Sci, Diadema, BrazilUniv São Paulo, Dept Parasitol, São Paulo, BrazilUniv São Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Phys & Earth Sci, Diadema, BrazilFAPESP: 2009/53256-7FAPESP: 2009/53889-0CNPq: 306668/2012-2FAPESP: 2011/195252-0Web of Science10engBiomed Central LtdMalaria JournalMalariaPlasmodiumAcute lung injury (ALI)Acute respiratory distress syndrome (ARDS)Transmission electron microscopyMurine modelUltrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndromeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000338889000001.pdfapplication/pdf1767366${dspace.ui.url}/bitstream/11600/37872/1/WOS000338889000001.pdf4b01d52b03731f04ee3c4d296beedc90MD51open accessTEXTWOS000338889000001.pdf.txtWOS000338889000001.pdf.txtExtracted texttext/plain34890${dspace.ui.url}/bitstream/11600/37872/9/WOS000338889000001.pdf.txt11ddf8367d16d0c865b0aecfc4a660a8MD59open accessTHUMBNAILWOS000338889000001.pdf.jpgWOS000338889000001.pdf.jpgIM Thumbnailimage/jpeg7764${dspace.ui.url}/bitstream/11600/37872/11/WOS000338889000001.pdf.jpgcd50c92f519684baab32eabd33c8ec66MD511open access11600/378722023-06-05 19:24:49.39open accessoai:repositorio.unifesp.br:11600/37872Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:24:49Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome |
title |
Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome |
spellingShingle |
Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome Aitken, Elizabeth H. Malaria Plasmodium Acute lung injury (ALI) Acute respiratory distress syndrome (ARDS) Transmission electron microscopy Murine model |
title_short |
Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome |
title_full |
Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome |
title_fullStr |
Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome |
title_full_unstemmed |
Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome |
title_sort |
Ultrastructure of the lung in a murine model of malaria-associated acute lung injury/acute respiratory distress syndrome |
author |
Aitken, Elizabeth H. |
author_facet |
Aitken, Elizabeth H. Negri, Elnara M. Barboza, Renato [UNIFESP] Lima, Maria Ri Alvarez, Jose M. Marinho, Claudio R. F. Caldini, Elia G. Epiphanio, Sabrina |
author_role |
author |
author2 |
Negri, Elnara M. Barboza, Renato [UNIFESP] Lima, Maria Ri Alvarez, Jose M. Marinho, Claudio R. F. Caldini, Elia G. Epiphanio, Sabrina |
author2_role |
author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Aitken, Elizabeth H. Negri, Elnara M. Barboza, Renato [UNIFESP] Lima, Maria Ri Alvarez, Jose M. Marinho, Claudio R. F. Caldini, Elia G. Epiphanio, Sabrina |
dc.subject.eng.fl_str_mv |
Malaria Plasmodium Acute lung injury (ALI) Acute respiratory distress syndrome (ARDS) Transmission electron microscopy Murine model |
topic |
Malaria Plasmodium Acute lung injury (ALI) Acute respiratory distress syndrome (ARDS) Transmission electron microscopy Murine model |
description |
Background: the mechanisms through which infection with Plasmodium spp. result in lung disease are largely unknown. Recently a number of mouse models have been developed to research malaria-associated lung injury but no detailed ultrastructure studies of the disease in its terminal stages in a murine model have yet been published. the goal was to perform an ultrastructural analysis of the lungs of mice that died with malaria-associated acute lung injury/acute respiratory distress syndrome to better determine the relevancy of the murine models and investigate the mechanism of disease.Methods: DBA/2 mice were infected with Plasmodium berghei strain ANKA. Mice had their lungs removed immediately after death, processed using standard methods and viewed by transmission electron microscopy (TEM).Results: Infected red blood cell: endothelium contact, swollen endothelium with distended cytoplasmic extensions and thickening of endothelium basement membrane were observed. Septa were thick and filled with congested capillaries and leukocytes and the alveolar spaces contained blood cells, oedema and cell debris.Conclusion: Results show that the lung ultrastructure of P. berghei ANKA-infected mice has similar features to what has been described in post-mortem TEM studies of lungs from individuals infected with Plasmodium falciparum. These data support the use of murine models to study malaria-associated acute lung injury. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-06-13 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:37:27Z |
dc.date.available.fl_str_mv |
2016-01-24T14:37:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Malaria Journal. London: Biomed Central Ltd, v. 13, 10 p., 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/37872 http://dx.doi.org/10.1186/1475-2875-13-230 |
dc.identifier.issn.none.fl_str_mv |
1475-2875 |
dc.identifier.file.none.fl_str_mv |
WOS000338889000001.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1186/1475-2875-13-230 |
dc.identifier.wos.none.fl_str_mv |
WOS:000338889000001 |
identifier_str_mv |
Malaria Journal. London: Biomed Central Ltd, v. 13, 10 p., 2014. 1475-2875 WOS000338889000001.pdf 10.1186/1475-2875-13-230 WOS:000338889000001 |
url |
http://repositorio.unifesp.br/handle/11600/37872 http://dx.doi.org/10.1186/1475-2875-13-230 |
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eng |
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Malaria Journal |
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Biomed Central Ltd |
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Biomed Central Ltd |
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