Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1016/j.autneu.2013.10.006 http://repositorio.unifesp.br/handle/11600/37335 |
Resumo: | Hyperhomocysteinemia (HHcy) is associated with cardiovascular disease, atherosclerosis and reactive oxygen species generation. Thus, our aim was to investigate whether there was an association between HHcy, blood pressure, autonomic control and liver oxidative stress. Male Wistar rats were divided into 2 groups and treated for 8 weeks: one group (control, CO) received tap water, while the other group (methionine, ME) was given a 100 mg/kg of methionine in water by gavage. Two catheters were implanted into the femoral artery and vein to record arterial pressure (AP) and heart rate (HR) and drug administration. Signals were recorded by a data acquisition system. Baroreflex sensitivity was evaluated by HR responses to AP changes induced by vasoactive drugs. HR variability and AP variability were performed by spectral analysis in time and frequency domains to evaluate the contribution of the sympathetic and parasympathetic modulation. Lipid peroxidation and antioxidant enzyme activities were evaluated by measuring superoxide dismutase, catalase and glutathione peroxidase in liver homogenates. the ME group presented a significant increase in systolic arterial pressure (118 +/- 9 vs 135 +/- 6 mm Hg), diastolic arterial pressure (81 +/- 6 vs. 92 +/- 4) and mean arterial pressure (95 +/- 7 vs. 106 +/- 6). in addition, pulse interval variability presented a significant decrease (41%), while the low frequency component of AP was significantly increased (delta P = 6.24 mmHg(2)) in the ME group. We also found a positive association between lipid peroxidation and cardiac sympathetic modulation, sympathetic and vagal modulation ratio and systolic pressure variability. Collectively, these findings showed that HHcy induced dysfunction of cardiovascular autonomic system and liver oxidative stress. (C) 2013 Elsevier B.V. All rights reserved. |
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Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in ratsHomocysteineAutonomic imbalanceLiver oxidative stressHyperhomocysteinemia (HHcy) is associated with cardiovascular disease, atherosclerosis and reactive oxygen species generation. Thus, our aim was to investigate whether there was an association between HHcy, blood pressure, autonomic control and liver oxidative stress. Male Wistar rats were divided into 2 groups and treated for 8 weeks: one group (control, CO) received tap water, while the other group (methionine, ME) was given a 100 mg/kg of methionine in water by gavage. Two catheters were implanted into the femoral artery and vein to record arterial pressure (AP) and heart rate (HR) and drug administration. Signals were recorded by a data acquisition system. Baroreflex sensitivity was evaluated by HR responses to AP changes induced by vasoactive drugs. HR variability and AP variability were performed by spectral analysis in time and frequency domains to evaluate the contribution of the sympathetic and parasympathetic modulation. Lipid peroxidation and antioxidant enzyme activities were evaluated by measuring superoxide dismutase, catalase and glutathione peroxidase in liver homogenates. the ME group presented a significant increase in systolic arterial pressure (118 +/- 9 vs 135 +/- 6 mm Hg), diastolic arterial pressure (81 +/- 6 vs. 92 +/- 4) and mean arterial pressure (95 +/- 7 vs. 106 +/- 6). in addition, pulse interval variability presented a significant decrease (41%), while the low frequency component of AP was significantly increased (delta P = 6.24 mmHg(2)) in the ME group. We also found a positive association between lipid peroxidation and cardiac sympathetic modulation, sympathetic and vagal modulation ratio and systolic pressure variability. Collectively, these findings showed that HHcy induced dysfunction of cardiovascular autonomic system and liver oxidative stress. (C) 2013 Elsevier B.V. All rights reserved.Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Fisiol, Lab Fisiol Cardiovasc, BR-90046900 Porto Alegre, RS, BrazilUniv São Paulo, Inst Coracao, Unidade Hipertensao, BR-05508 São Paulo, BrazilUniv Fed Ciencias Saude Porto Alegre, BR-90050170 Porto Alegre, RS, BrazilUniversidade Federal de São Paulo, Dept Biociencias, São Paulo, BrazilUniv Fed Rio de Janeiro, Inst Nutr Josue Castro, BR-21941 Rio de Janeiro, BrazilUniv Santo Amaro, Programa Posgrad Ciencias Saude, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biociencias, São Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAPERGSElsevier B.V.Univ Fed Rio Grande do SulUniversidade de São Paulo (USP)Univ Fed Ciencias Saude Porto AlegreUniversidade Federal de São Paulo (UNIFESP)Universidade Federal do Rio de Janeiro (UFRJ)Univ Santo AmaroMendes, Roberta HackMostarda, Cristiano [UNIFESP]Candido, Georgia Orsi [UNIFESP]Moraes-Silva, Ivana CinthyaD'Almeida, Vânia[UNIFESP]Bello-Klein, Adriane [UNIFESP]Irigoyen, Maria Claudia [UNIFESP]Rigatto, Katya2016-01-24T14:35:10Z2016-01-24T14:35:10Z2014-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion43-47application/pdfhttp://dx.doi.org/10.1016/j.autneu.2013.10.006Autonomic Neuroscience-basic & Clinical. Amsterdam: Elsevier B.V., v. 180, p. 43-47, 2014.10.1016/j.autneu.2013.10.006WOS000330917500007.pdf1566-0702http://repositorio.unifesp.br/handle/11600/37335WOS:000330917500007engAutonomic Neuroscience-basic & Clinicalinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T18:31:19Zoai:repositorio.unifesp.br/:11600/37335Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T18:31:19Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats |
title |
Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats |
spellingShingle |
Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats Mendes, Roberta Hack Homocysteine Autonomic imbalance Liver oxidative stress |
title_short |
Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats |
title_full |
Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats |
title_fullStr |
Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats |
title_full_unstemmed |
Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats |
title_sort |
Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats |
author |
Mendes, Roberta Hack |
author_facet |
Mendes, Roberta Hack Mostarda, Cristiano [UNIFESP] Candido, Georgia Orsi [UNIFESP] Moraes-Silva, Ivana Cinthya D'Almeida, Vânia[UNIFESP] Bello-Klein, Adriane [UNIFESP] Irigoyen, Maria Claudia [UNIFESP] Rigatto, Katya |
author_role |
author |
author2 |
Mostarda, Cristiano [UNIFESP] Candido, Georgia Orsi [UNIFESP] Moraes-Silva, Ivana Cinthya D'Almeida, Vânia[UNIFESP] Bello-Klein, Adriane [UNIFESP] Irigoyen, Maria Claudia [UNIFESP] Rigatto, Katya |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Rio Grande do Sul Universidade de São Paulo (USP) Univ Fed Ciencias Saude Porto Alegre Universidade Federal de São Paulo (UNIFESP) Universidade Federal do Rio de Janeiro (UFRJ) Univ Santo Amaro |
dc.contributor.author.fl_str_mv |
Mendes, Roberta Hack Mostarda, Cristiano [UNIFESP] Candido, Georgia Orsi [UNIFESP] Moraes-Silva, Ivana Cinthya D'Almeida, Vânia[UNIFESP] Bello-Klein, Adriane [UNIFESP] Irigoyen, Maria Claudia [UNIFESP] Rigatto, Katya |
dc.subject.por.fl_str_mv |
Homocysteine Autonomic imbalance Liver oxidative stress |
topic |
Homocysteine Autonomic imbalance Liver oxidative stress |
description |
Hyperhomocysteinemia (HHcy) is associated with cardiovascular disease, atherosclerosis and reactive oxygen species generation. Thus, our aim was to investigate whether there was an association between HHcy, blood pressure, autonomic control and liver oxidative stress. Male Wistar rats were divided into 2 groups and treated for 8 weeks: one group (control, CO) received tap water, while the other group (methionine, ME) was given a 100 mg/kg of methionine in water by gavage. Two catheters were implanted into the femoral artery and vein to record arterial pressure (AP) and heart rate (HR) and drug administration. Signals were recorded by a data acquisition system. Baroreflex sensitivity was evaluated by HR responses to AP changes induced by vasoactive drugs. HR variability and AP variability were performed by spectral analysis in time and frequency domains to evaluate the contribution of the sympathetic and parasympathetic modulation. Lipid peroxidation and antioxidant enzyme activities were evaluated by measuring superoxide dismutase, catalase and glutathione peroxidase in liver homogenates. the ME group presented a significant increase in systolic arterial pressure (118 +/- 9 vs 135 +/- 6 mm Hg), diastolic arterial pressure (81 +/- 6 vs. 92 +/- 4) and mean arterial pressure (95 +/- 7 vs. 106 +/- 6). in addition, pulse interval variability presented a significant decrease (41%), while the low frequency component of AP was significantly increased (delta P = 6.24 mmHg(2)) in the ME group. We also found a positive association between lipid peroxidation and cardiac sympathetic modulation, sympathetic and vagal modulation ratio and systolic pressure variability. Collectively, these findings showed that HHcy induced dysfunction of cardiovascular autonomic system and liver oxidative stress. (C) 2013 Elsevier B.V. All rights reserved. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-01 2016-01-24T14:35:10Z 2016-01-24T14:35:10Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.autneu.2013.10.006 Autonomic Neuroscience-basic & Clinical. Amsterdam: Elsevier B.V., v. 180, p. 43-47, 2014. 10.1016/j.autneu.2013.10.006 WOS000330917500007.pdf 1566-0702 http://repositorio.unifesp.br/handle/11600/37335 WOS:000330917500007 |
url |
http://dx.doi.org/10.1016/j.autneu.2013.10.006 http://repositorio.unifesp.br/handle/11600/37335 |
identifier_str_mv |
Autonomic Neuroscience-basic & Clinical. Amsterdam: Elsevier B.V., v. 180, p. 43-47, 2014. 10.1016/j.autneu.2013.10.006 WOS000330917500007.pdf 1566-0702 WOS:000330917500007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Autonomic Neuroscience-basic & Clinical |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy |
dc.format.none.fl_str_mv |
43-47 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268399454257152 |