Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats

Detalhes bibliográficos
Autor(a) principal: Mendes, Roberta Hack
Data de Publicação: 2014
Outros Autores: Mostarda, Cristiano [UNIFESP], Candido, Georgia Orsi [UNIFESP], Moraes-Silva, Ivana Cinthya, D'Almeida, Vânia[UNIFESP], Bello-Klein, Adriane [UNIFESP], Irigoyen, Maria Claudia [UNIFESP], Rigatto, Katya
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1016/j.autneu.2013.10.006
http://repositorio.unifesp.br/handle/11600/37335
Resumo: Hyperhomocysteinemia (HHcy) is associated with cardiovascular disease, atherosclerosis and reactive oxygen species generation. Thus, our aim was to investigate whether there was an association between HHcy, blood pressure, autonomic control and liver oxidative stress. Male Wistar rats were divided into 2 groups and treated for 8 weeks: one group (control, CO) received tap water, while the other group (methionine, ME) was given a 100 mg/kg of methionine in water by gavage. Two catheters were implanted into the femoral artery and vein to record arterial pressure (AP) and heart rate (HR) and drug administration. Signals were recorded by a data acquisition system. Baroreflex sensitivity was evaluated by HR responses to AP changes induced by vasoactive drugs. HR variability and AP variability were performed by spectral analysis in time and frequency domains to evaluate the contribution of the sympathetic and parasympathetic modulation. Lipid peroxidation and antioxidant enzyme activities were evaluated by measuring superoxide dismutase, catalase and glutathione peroxidase in liver homogenates. the ME group presented a significant increase in systolic arterial pressure (118 +/- 9 vs 135 +/- 6 mm Hg), diastolic arterial pressure (81 +/- 6 vs. 92 +/- 4) and mean arterial pressure (95 +/- 7 vs. 106 +/- 6). in addition, pulse interval variability presented a significant decrease (41%), while the low frequency component of AP was significantly increased (delta P = 6.24 mmHg(2)) in the ME group. We also found a positive association between lipid peroxidation and cardiac sympathetic modulation, sympathetic and vagal modulation ratio and systolic pressure variability. Collectively, these findings showed that HHcy induced dysfunction of cardiovascular autonomic system and liver oxidative stress. (C) 2013 Elsevier B.V. All rights reserved.
id UFSP_0d4f96bab6d98e8237a9978df9270f17
oai_identifier_str oai:repositorio.unifesp.br/:11600/37335
network_acronym_str UFSP
network_name_str Repositório Institucional da UNIFESP
repository_id_str 3465
spelling Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in ratsHomocysteineAutonomic imbalanceLiver oxidative stressHyperhomocysteinemia (HHcy) is associated with cardiovascular disease, atherosclerosis and reactive oxygen species generation. Thus, our aim was to investigate whether there was an association between HHcy, blood pressure, autonomic control and liver oxidative stress. Male Wistar rats were divided into 2 groups and treated for 8 weeks: one group (control, CO) received tap water, while the other group (methionine, ME) was given a 100 mg/kg of methionine in water by gavage. Two catheters were implanted into the femoral artery and vein to record arterial pressure (AP) and heart rate (HR) and drug administration. Signals were recorded by a data acquisition system. Baroreflex sensitivity was evaluated by HR responses to AP changes induced by vasoactive drugs. HR variability and AP variability were performed by spectral analysis in time and frequency domains to evaluate the contribution of the sympathetic and parasympathetic modulation. Lipid peroxidation and antioxidant enzyme activities were evaluated by measuring superoxide dismutase, catalase and glutathione peroxidase in liver homogenates. the ME group presented a significant increase in systolic arterial pressure (118 +/- 9 vs 135 +/- 6 mm Hg), diastolic arterial pressure (81 +/- 6 vs. 92 +/- 4) and mean arterial pressure (95 +/- 7 vs. 106 +/- 6). in addition, pulse interval variability presented a significant decrease (41%), while the low frequency component of AP was significantly increased (delta P = 6.24 mmHg(2)) in the ME group. We also found a positive association between lipid peroxidation and cardiac sympathetic modulation, sympathetic and vagal modulation ratio and systolic pressure variability. Collectively, these findings showed that HHcy induced dysfunction of cardiovascular autonomic system and liver oxidative stress. (C) 2013 Elsevier B.V. All rights reserved.Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Fisiol, Lab Fisiol Cardiovasc, BR-90046900 Porto Alegre, RS, BrazilUniv São Paulo, Inst Coracao, Unidade Hipertensao, BR-05508 São Paulo, BrazilUniv Fed Ciencias Saude Porto Alegre, BR-90050170 Porto Alegre, RS, BrazilUniversidade Federal de São Paulo, Dept Biociencias, São Paulo, BrazilUniv Fed Rio de Janeiro, Inst Nutr Josue Castro, BR-21941 Rio de Janeiro, BrazilUniv Santo Amaro, Programa Posgrad Ciencias Saude, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biociencias, São Paulo, BrazilWeb of ScienceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FAPERGSElsevier B.V.Univ Fed Rio Grande do SulUniversidade de São Paulo (USP)Univ Fed Ciencias Saude Porto AlegreUniversidade Federal de São Paulo (UNIFESP)Universidade Federal do Rio de Janeiro (UFRJ)Univ Santo AmaroMendes, Roberta HackMostarda, Cristiano [UNIFESP]Candido, Georgia Orsi [UNIFESP]Moraes-Silva, Ivana CinthyaD'Almeida, Vânia[UNIFESP]Bello-Klein, Adriane [UNIFESP]Irigoyen, Maria Claudia [UNIFESP]Rigatto, Katya2016-01-24T14:35:10Z2016-01-24T14:35:10Z2014-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion43-47application/pdfhttp://dx.doi.org/10.1016/j.autneu.2013.10.006Autonomic Neuroscience-basic & Clinical. Amsterdam: Elsevier B.V., v. 180, p. 43-47, 2014.10.1016/j.autneu.2013.10.006WOS000330917500007.pdf1566-0702http://repositorio.unifesp.br/handle/11600/37335WOS:000330917500007engAutonomic Neuroscience-basic & Clinicalinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-31T18:31:19Zoai:repositorio.unifesp.br/:11600/37335Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-31T18:31:19Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats
title Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats
spellingShingle Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats
Mendes, Roberta Hack
Homocysteine
Autonomic imbalance
Liver oxidative stress
title_short Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats
title_full Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats
title_fullStr Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats
title_full_unstemmed Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats
title_sort Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats
author Mendes, Roberta Hack
author_facet Mendes, Roberta Hack
Mostarda, Cristiano [UNIFESP]
Candido, Georgia Orsi [UNIFESP]
Moraes-Silva, Ivana Cinthya
D'Almeida, Vânia[UNIFESP]
Bello-Klein, Adriane [UNIFESP]
Irigoyen, Maria Claudia [UNIFESP]
Rigatto, Katya
author_role author
author2 Mostarda, Cristiano [UNIFESP]
Candido, Georgia Orsi [UNIFESP]
Moraes-Silva, Ivana Cinthya
D'Almeida, Vânia[UNIFESP]
Bello-Klein, Adriane [UNIFESP]
Irigoyen, Maria Claudia [UNIFESP]
Rigatto, Katya
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Fed Rio Grande do Sul
Universidade de São Paulo (USP)
Univ Fed Ciencias Saude Porto Alegre
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do Rio de Janeiro (UFRJ)
Univ Santo Amaro
dc.contributor.author.fl_str_mv Mendes, Roberta Hack
Mostarda, Cristiano [UNIFESP]
Candido, Georgia Orsi [UNIFESP]
Moraes-Silva, Ivana Cinthya
D'Almeida, Vânia[UNIFESP]
Bello-Klein, Adriane [UNIFESP]
Irigoyen, Maria Claudia [UNIFESP]
Rigatto, Katya
dc.subject.por.fl_str_mv Homocysteine
Autonomic imbalance
Liver oxidative stress
topic Homocysteine
Autonomic imbalance
Liver oxidative stress
description Hyperhomocysteinemia (HHcy) is associated with cardiovascular disease, atherosclerosis and reactive oxygen species generation. Thus, our aim was to investigate whether there was an association between HHcy, blood pressure, autonomic control and liver oxidative stress. Male Wistar rats were divided into 2 groups and treated for 8 weeks: one group (control, CO) received tap water, while the other group (methionine, ME) was given a 100 mg/kg of methionine in water by gavage. Two catheters were implanted into the femoral artery and vein to record arterial pressure (AP) and heart rate (HR) and drug administration. Signals were recorded by a data acquisition system. Baroreflex sensitivity was evaluated by HR responses to AP changes induced by vasoactive drugs. HR variability and AP variability were performed by spectral analysis in time and frequency domains to evaluate the contribution of the sympathetic and parasympathetic modulation. Lipid peroxidation and antioxidant enzyme activities were evaluated by measuring superoxide dismutase, catalase and glutathione peroxidase in liver homogenates. the ME group presented a significant increase in systolic arterial pressure (118 +/- 9 vs 135 +/- 6 mm Hg), diastolic arterial pressure (81 +/- 6 vs. 92 +/- 4) and mean arterial pressure (95 +/- 7 vs. 106 +/- 6). in addition, pulse interval variability presented a significant decrease (41%), while the low frequency component of AP was significantly increased (delta P = 6.24 mmHg(2)) in the ME group. We also found a positive association between lipid peroxidation and cardiac sympathetic modulation, sympathetic and vagal modulation ratio and systolic pressure variability. Collectively, these findings showed that HHcy induced dysfunction of cardiovascular autonomic system and liver oxidative stress. (C) 2013 Elsevier B.V. All rights reserved.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-01
2016-01-24T14:35:10Z
2016-01-24T14:35:10Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.autneu.2013.10.006
Autonomic Neuroscience-basic & Clinical. Amsterdam: Elsevier B.V., v. 180, p. 43-47, 2014.
10.1016/j.autneu.2013.10.006
WOS000330917500007.pdf
1566-0702
http://repositorio.unifesp.br/handle/11600/37335
WOS:000330917500007
url http://dx.doi.org/10.1016/j.autneu.2013.10.006
http://repositorio.unifesp.br/handle/11600/37335
identifier_str_mv Autonomic Neuroscience-basic & Clinical. Amsterdam: Elsevier B.V., v. 180, p. 43-47, 2014.
10.1016/j.autneu.2013.10.006
WOS000330917500007.pdf
1566-0702
WOS:000330917500007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Autonomic Neuroscience-basic & Clinical
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 43-47
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268399454257152