Intraspecies Variation in Trypanosoma cruzi GPI-Mucins: Biological Activities and Differential Expression of alpha-Galactosyl Residues

• Autor(a) principal: Soares, Rodrigo Pedro
• Data de Publicação: 2012
• Outros Autores: Torrecilhas, Ana Claudia Trocoli [UNIFESP], Assis, Rafael Ramiro de, Rocha, Marcele Neves, Castro, Felipe Augusto Moura e, Freitas, Gustavo Ferreira de, Murta, Silvane Maria, Santos, Sara Lopes dos, Marques, Alexandre Ferreira, Almeida, Igor Correia de, Romanha, Alvaro Jose
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://repositorio.unifesp.br/handle/11600/35027; http://dx.doi.org/10.4269/ajtmh.2012.12-0015

The glycosylphosphatidylinositol (GPI)-anchored mucins of Trypanosoma cruzi trypomastigotes play an important immunomodulatory role during the course of Chagas disease. Here, some biological activities of tGPI-mucins from four T cruzi isolates, including benznidazole-susceptible (BZS-Y), benznidazole-resistant (BZR-Y), CL, and Colombiana, were evaluated. GPI-mucins were able to differentially trigger the production of interleukin-12 and nitric oxide in BALB/c macrophages and modulate LLC-MK2 cell invasion. the significance of these variations was assessed after analysis of the terminal alpha-galactosyl residues. Enzymatic treatment with alpha-galactosidase indicated a differential expression of O-linked alpha-galactosyl residues among the strains, with higher expression of this sugar in BZS-Y and BZR-Y T cruzi populations followed by Colombiana and CL. Unweighted pair group method analysis of the carbohydrate anchor profile and biological parameters allowed the clustering of two groups. One group includes Y and CL strains (T cruzi II and VI), and the other group is represented by Colombiana strain (T. cruzi I).