Kinin B1 and B2 receptors are overexpressed in the hippocampus of humans with temporal lobe epilepsy
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/29323 http://dx.doi.org/10.1002/hipo.20239 |
Resumo: | Molecular biology tools have been employed to investigate the participation of peptides in human temporal lobe epilepsy ME). Active polypeptides and their receptors have been related to several brain processes, such as inflammation, apoptosis, brain development, K+ and Ca2+ channels' activation, cellular growth, and induction of neuronal differentiation. Previous works have shown a neuroprotector effect for kinin B2 receptor and a deleterious, pro-epileptogenic action for kinin B1 receptor in animal models of TLE. the present work was delineated to analyze the kinin B1 and B2 receptors expression in the hippocampus of patients presenting refractory mesial TLE. the hippocampi were removed during the patients surgery in a procedure used for seizure control and compared with tissues obtained after autopsy. Nissl staining was performed to study the tissue morphology and immunohistochemistry, and Western blot was used to compare the distribution and levels of both receptors in the hippocampus. in addition, real time PCR was employed to analyze the gene expression of these receptors. Nissl staining showed sclerotic hippocampi with hilar, granular, and pyramidal cell loss in TLE patients. Immunohistochemistry and Western blot analyses showed increased expression of kinin B1 and B2 receptors but the real-time PCR data demonstrated increased mRNA level only for kinin B2 receptors, when compared with controls. These data show for the first time a relationship between human TLE and the kallikrein-kinin system, confirming ours previous results, obtained from experimental models of epilepsy. (c) 2006 Wiley-Liss, Inc. |
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Perosa, Sandra Regina [UNIFESP]Argañaraz, Gustavo Adolfo [UNIFESP]Goto, Eduardo Massatoshi [UNIFESP]Costa, Luciana Gilbert Pessoa [UNIFESP]Konno, Ana Carla [UNIFESP]Varella, Pedro Paulo Vasconcellos [UNIFESP]Santiago, Joselita Ferreira Carvalho [UNIFESP]Pesquero, Joao Bosco [UNIFESP]Canzian, MauroAmado, Débora [UNIFESP]Yacubian, Elza Márcia Targas [UNIFESP]Carrete Junior, Henrique [UNIFESP]Centeno, Ricardo Silva [UNIFESP]Cavalheiro, Esper Abrão [UNIFESP]Silva, Jose AntonioNaffah-Mazzacoratti, Maria da Graca [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Ctr Univ Nove de Julho2016-01-24T12:41:40Z2016-01-24T12:41:40Z2007-01-01Hippocampus. Hoboken: Wiley-liss, v. 17, n. 1, p. 26-33, 2007.1050-9631http://repositorio.unifesp.br/handle/11600/29323http://dx.doi.org/10.1002/hipo.2023910.1002/hipo.20239WOS:000243332000004Molecular biology tools have been employed to investigate the participation of peptides in human temporal lobe epilepsy ME). Active polypeptides and their receptors have been related to several brain processes, such as inflammation, apoptosis, brain development, K+ and Ca2+ channels' activation, cellular growth, and induction of neuronal differentiation. Previous works have shown a neuroprotector effect for kinin B2 receptor and a deleterious, pro-epileptogenic action for kinin B1 receptor in animal models of TLE. the present work was delineated to analyze the kinin B1 and B2 receptors expression in the hippocampus of patients presenting refractory mesial TLE. the hippocampi were removed during the patients surgery in a procedure used for seizure control and compared with tissues obtained after autopsy. Nissl staining was performed to study the tissue morphology and immunohistochemistry, and Western blot was used to compare the distribution and levels of both receptors in the hippocampus. in addition, real time PCR was employed to analyze the gene expression of these receptors. Nissl staining showed sclerotic hippocampi with hilar, granular, and pyramidal cell loss in TLE patients. Immunohistochemistry and Western blot analyses showed increased expression of kinin B1 and B2 receptors but the real-time PCR data demonstrated increased mRNA level only for kinin B2 receptors, when compared with controls. These data show for the first time a relationship between human TLE and the kallikrein-kinin system, confirming ours previous results, obtained from experimental models of epilepsy. (c) 2006 Wiley-Liss, Inc.Universidade Federal de São Paulo, Dept Expt Neurol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Patol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biofis, São Paulo, BrazilFCMUSP, Dept Anat Patol Incor, São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Neurol Clin, Dept Neurol Neurocirurgia, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Diagnost Imagem, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, BrazilCtr Univ Nove de Julho, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Expt Neurol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Patol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biofis, São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Neurol Clin, Dept Neurol Neurocirurgia, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Diagnost Imagem, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Bioquim, São Paulo, BrazilWeb of Science26-33engWiley-BlackwellHippocampushttp://olabout.wiley.com/WileyCDA/Section/id-406071.htmlinfo:eu-repo/semantics/openAccesskinin B1 and B2 receptorshuman temporal lobe epilepsyinflammationKinin B1 and B2 receptors are overexpressed in the hippocampus of humans with temporal lobe epilepsyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlereponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/293232023-01-30 22:17:46.52metadata only accessoai:repositorio.unifesp.br:11600/29323Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-05-25T12:44:41.808793Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Kinin B1 and B2 receptors are overexpressed in the hippocampus of humans with temporal lobe epilepsy |
title |
Kinin B1 and B2 receptors are overexpressed in the hippocampus of humans with temporal lobe epilepsy |
spellingShingle |
Kinin B1 and B2 receptors are overexpressed in the hippocampus of humans with temporal lobe epilepsy Perosa, Sandra Regina [UNIFESP] kinin B1 and B2 receptors human temporal lobe epilepsy inflammation |
title_short |
Kinin B1 and B2 receptors are overexpressed in the hippocampus of humans with temporal lobe epilepsy |
title_full |
Kinin B1 and B2 receptors are overexpressed in the hippocampus of humans with temporal lobe epilepsy |
title_fullStr |
Kinin B1 and B2 receptors are overexpressed in the hippocampus of humans with temporal lobe epilepsy |
title_full_unstemmed |
Kinin B1 and B2 receptors are overexpressed in the hippocampus of humans with temporal lobe epilepsy |
title_sort |
Kinin B1 and B2 receptors are overexpressed in the hippocampus of humans with temporal lobe epilepsy |
author |
Perosa, Sandra Regina [UNIFESP] |
author_facet |
Perosa, Sandra Regina [UNIFESP] Argañaraz, Gustavo Adolfo [UNIFESP] Goto, Eduardo Massatoshi [UNIFESP] Costa, Luciana Gilbert Pessoa [UNIFESP] Konno, Ana Carla [UNIFESP] Varella, Pedro Paulo Vasconcellos [UNIFESP] Santiago, Joselita Ferreira Carvalho [UNIFESP] Pesquero, Joao Bosco [UNIFESP] Canzian, Mauro Amado, Débora [UNIFESP] Yacubian, Elza Márcia Targas [UNIFESP] Carrete Junior, Henrique [UNIFESP] Centeno, Ricardo Silva [UNIFESP] Cavalheiro, Esper Abrão [UNIFESP] Silva, Jose Antonio Naffah-Mazzacoratti, Maria da Graca [UNIFESP] |
author_role |
author |
author2 |
Argañaraz, Gustavo Adolfo [UNIFESP] Goto, Eduardo Massatoshi [UNIFESP] Costa, Luciana Gilbert Pessoa [UNIFESP] Konno, Ana Carla [UNIFESP] Varella, Pedro Paulo Vasconcellos [UNIFESP] Santiago, Joselita Ferreira Carvalho [UNIFESP] Pesquero, Joao Bosco [UNIFESP] Canzian, Mauro Amado, Débora [UNIFESP] Yacubian, Elza Márcia Targas [UNIFESP] Carrete Junior, Henrique [UNIFESP] Centeno, Ricardo Silva [UNIFESP] Cavalheiro, Esper Abrão [UNIFESP] Silva, Jose Antonio Naffah-Mazzacoratti, Maria da Graca [UNIFESP] |
author2_role |
author author author author author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) Ctr Univ Nove de Julho |
dc.contributor.author.fl_str_mv |
Perosa, Sandra Regina [UNIFESP] Argañaraz, Gustavo Adolfo [UNIFESP] Goto, Eduardo Massatoshi [UNIFESP] Costa, Luciana Gilbert Pessoa [UNIFESP] Konno, Ana Carla [UNIFESP] Varella, Pedro Paulo Vasconcellos [UNIFESP] Santiago, Joselita Ferreira Carvalho [UNIFESP] Pesquero, Joao Bosco [UNIFESP] Canzian, Mauro Amado, Débora [UNIFESP] Yacubian, Elza Márcia Targas [UNIFESP] Carrete Junior, Henrique [UNIFESP] Centeno, Ricardo Silva [UNIFESP] Cavalheiro, Esper Abrão [UNIFESP] Silva, Jose Antonio Naffah-Mazzacoratti, Maria da Graca [UNIFESP] |
dc.subject.eng.fl_str_mv |
kinin B1 and B2 receptors human temporal lobe epilepsy inflammation |
topic |
kinin B1 and B2 receptors human temporal lobe epilepsy inflammation |
description |
Molecular biology tools have been employed to investigate the participation of peptides in human temporal lobe epilepsy ME). Active polypeptides and their receptors have been related to several brain processes, such as inflammation, apoptosis, brain development, K+ and Ca2+ channels' activation, cellular growth, and induction of neuronal differentiation. Previous works have shown a neuroprotector effect for kinin B2 receptor and a deleterious, pro-epileptogenic action for kinin B1 receptor in animal models of TLE. the present work was delineated to analyze the kinin B1 and B2 receptors expression in the hippocampus of patients presenting refractory mesial TLE. the hippocampi were removed during the patients surgery in a procedure used for seizure control and compared with tissues obtained after autopsy. Nissl staining was performed to study the tissue morphology and immunohistochemistry, and Western blot was used to compare the distribution and levels of both receptors in the hippocampus. in addition, real time PCR was employed to analyze the gene expression of these receptors. Nissl staining showed sclerotic hippocampi with hilar, granular, and pyramidal cell loss in TLE patients. Immunohistochemistry and Western blot analyses showed increased expression of kinin B1 and B2 receptors but the real-time PCR data demonstrated increased mRNA level only for kinin B2 receptors, when compared with controls. These data show for the first time a relationship between human TLE and the kallikrein-kinin system, confirming ours previous results, obtained from experimental models of epilepsy. (c) 2006 Wiley-Liss, Inc. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-01-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T12:41:40Z |
dc.date.available.fl_str_mv |
2016-01-24T12:41:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Hippocampus. Hoboken: Wiley-liss, v. 17, n. 1, p. 26-33, 2007. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/29323 http://dx.doi.org/10.1002/hipo.20239 |
dc.identifier.issn.none.fl_str_mv |
1050-9631 |
dc.identifier.doi.none.fl_str_mv |
10.1002/hipo.20239 |
dc.identifier.wos.none.fl_str_mv |
WOS:000243332000004 |
identifier_str_mv |
Hippocampus. Hoboken: Wiley-liss, v. 17, n. 1, p. 26-33, 2007. 1050-9631 10.1002/hipo.20239 WOS:000243332000004 |
url |
http://repositorio.unifesp.br/handle/11600/29323 http://dx.doi.org/10.1002/hipo.20239 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Hippocampus |
dc.rights.driver.fl_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://olabout.wiley.com/WileyCDA/Section/id-406071.html |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
26-33 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
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1783460334726545408 |