The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission

Detalhes bibliográficos
Autor(a) principal: Borja-oliveira, C.r.
Data de Publicação: 2003
Outros Autores: Durigon, A.m., Vallin, A.c.c. [UNIFESP], Toyama, M.h., Souccar, Caden [UNIFESP], Marangoni, S., Rodrigues-simioni, L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2003000500009
http://repositorio.unifesp.br/handle/11600/1708
Resumo: The neuromuscular effects of Bothrops neuwiedii pauloensis (jararaca-pintada) venom were studied on isolated chick biventer cervicis nerve-muscle preparations. Venom concentrations of 5-50 µg/ml produced an initial inhibition and a secondary increase of indirectly evoked twitches followed by a progressive concentration-dependent and irreversible neuromuscular blockade. At venom concentrations of 1-20 µg/ml, the responses to 13.4 mM KCl were inhibited whereas those to 110 µM acetylcholine alone and cumulative concentrations of 1 µM to 10 mM were unaffected. At venom concentrations higher than 50 µg/ml, there was pronounced muscle contracture with inhibition of the responses to acetylcholine, KCl and direct stimulation. At 20-24ºC, the venom (50 µg/ml) produced only partial neuromuscular blockade (30.7 ± 8.0%, N = 3) after 120 min and the initial inhibition and the secondary increase of the twitch responses caused by the venom were prolonged and pronounced and the response to KCl was unchanged. These results indicate that B.n. pauloensis venom is neurotoxic, acting primarily at presynaptic sites, and that enzyme activity may be involved in this pharmacological action.
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spelling The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmissionChick biventer cervicisMyotoxicityNeurotoxicityPhospholipase A2Presynaptic actionThe neuromuscular effects of Bothrops neuwiedii pauloensis (jararaca-pintada) venom were studied on isolated chick biventer cervicis nerve-muscle preparations. Venom concentrations of 5-50 µg/ml produced an initial inhibition and a secondary increase of indirectly evoked twitches followed by a progressive concentration-dependent and irreversible neuromuscular blockade. At venom concentrations of 1-20 µg/ml, the responses to 13.4 mM KCl were inhibited whereas those to 110 µM acetylcholine alone and cumulative concentrations of 1 µM to 10 mM were unaffected. At venom concentrations higher than 50 µg/ml, there was pronounced muscle contracture with inhibition of the responses to acetylcholine, KCl and direct stimulation. At 20-24ºC, the venom (50 µg/ml) produced only partial neuromuscular blockade (30.7 ± 8.0%, N = 3) after 120 min and the initial inhibition and the secondary increase of the twitch responses caused by the venom were prolonged and pronounced and the response to KCl was unchanged. These results indicate that B.n. pauloensis venom is neurotoxic, acting primarily at presynaptic sites, and that enzyme activity may be involved in this pharmacological action.Universidade Estadual de Campinas Faculdade de Ciências Médicas Departamento de FarmacologiaUniversidade Estadual de Campinas Instituto de Biologia Departamento de BioquímicaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de FarmacologiaUNIFESP, EPM, Depto. de FarmacologiaSciELOAssociação Brasileira de Divulgação CientíficaUniversidade Estadual de Campinas (UNICAMP)Universidade Federal de São Paulo (UNIFESP)Borja-oliveira, C.r.Durigon, A.m.Vallin, A.c.c. [UNIFESP]Toyama, M.h.Souccar, Caden [UNIFESP]Marangoni, S.Rodrigues-simioni, L.2015-06-14T13:30:00Z2015-06-14T13:30:00Z2003-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion617-624application/pdfhttp://dx.doi.org/10.1590/S0100-879X2003000500009Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 5, p. 617-624, 2003.10.1590/S0100-879X2003000500009S0100-879X2003000500009.pdf0100-879XS0100-879X2003000500009http://repositorio.unifesp.br/handle/11600/1708WOS:000183100000009engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T17:35:55Zoai:repositorio.unifesp.br/:11600/1708Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T17:35:55Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission
title The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission
spellingShingle The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission
Borja-oliveira, C.r.
Chick biventer cervicis
Myotoxicity
Neurotoxicity
Phospholipase A2
Presynaptic action
title_short The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission
title_full The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission
title_fullStr The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission
title_full_unstemmed The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission
title_sort The pharmacological effect of Bothrops neuwiedii pauloensis (jararaca-pintada) snake venom on avian neuromuscular transmission
author Borja-oliveira, C.r.
author_facet Borja-oliveira, C.r.
Durigon, A.m.
Vallin, A.c.c. [UNIFESP]
Toyama, M.h.
Souccar, Caden [UNIFESP]
Marangoni, S.
Rodrigues-simioni, L.
author_role author
author2 Durigon, A.m.
Vallin, A.c.c. [UNIFESP]
Toyama, M.h.
Souccar, Caden [UNIFESP]
Marangoni, S.
Rodrigues-simioni, L.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Borja-oliveira, C.r.
Durigon, A.m.
Vallin, A.c.c. [UNIFESP]
Toyama, M.h.
Souccar, Caden [UNIFESP]
Marangoni, S.
Rodrigues-simioni, L.
dc.subject.por.fl_str_mv Chick biventer cervicis
Myotoxicity
Neurotoxicity
Phospholipase A2
Presynaptic action
topic Chick biventer cervicis
Myotoxicity
Neurotoxicity
Phospholipase A2
Presynaptic action
description The neuromuscular effects of Bothrops neuwiedii pauloensis (jararaca-pintada) venom were studied on isolated chick biventer cervicis nerve-muscle preparations. Venom concentrations of 5-50 µg/ml produced an initial inhibition and a secondary increase of indirectly evoked twitches followed by a progressive concentration-dependent and irreversible neuromuscular blockade. At venom concentrations of 1-20 µg/ml, the responses to 13.4 mM KCl were inhibited whereas those to 110 µM acetylcholine alone and cumulative concentrations of 1 µM to 10 mM were unaffected. At venom concentrations higher than 50 µg/ml, there was pronounced muscle contracture with inhibition of the responses to acetylcholine, KCl and direct stimulation. At 20-24ºC, the venom (50 µg/ml) produced only partial neuromuscular blockade (30.7 ± 8.0%, N = 3) after 120 min and the initial inhibition and the secondary increase of the twitch responses caused by the venom were prolonged and pronounced and the response to KCl was unchanged. These results indicate that B.n. pauloensis venom is neurotoxic, acting primarily at presynaptic sites, and that enzyme activity may be involved in this pharmacological action.
publishDate 2003
dc.date.none.fl_str_mv 2003-05-01
2015-06-14T13:30:00Z
2015-06-14T13:30:00Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2003000500009
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 5, p. 617-624, 2003.
10.1590/S0100-879X2003000500009
S0100-879X2003000500009.pdf
0100-879X
S0100-879X2003000500009
http://repositorio.unifesp.br/handle/11600/1708
WOS:000183100000009
url http://dx.doi.org/10.1590/S0100-879X2003000500009
http://repositorio.unifesp.br/handle/11600/1708
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 5, p. 617-624, 2003.
10.1590/S0100-879X2003000500009
S0100-879X2003000500009.pdf
0100-879X
S0100-879X2003000500009
WOS:000183100000009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 617-624
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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