Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages

Detalhes bibliográficos
Autor(a) principal: Silva, Reinaldo Correia [UNIFESP]
Data de Publicação: 2013
Outros Autores: Landgraf, Maristella de Almeida [UNIFESP], Correa-Costa, Matheus, Semedo, Patricia [UNIFESP], Cenedeze, Marcos Antonio [UNIFESP], Pacheco-Silva, Alvaro [UNIFESP], Landgraf, Richardt Gama [UNIFESP], Câmara, Niels Olsen Saraiva [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1159/000343359
http://repositorio.unifesp.br/handle/11600/35715
Resumo: Background/Aims: Renal ischaemia-reperfusion injury (IRI) is a systemic inflammatory process in which Th1 responses predominate affecting other organs including the lungs. the present study explored the phagocytic and microbicidal capacity of macrophages in rats with lung inflammation that underwent IRI. Methods: the alveolar macrophages of rats sensitised to OVA were evaluated for phagocytosis and bacterial killing 24h after antigen challenge in animals with or without prior submission to 60 min of renal ischaemia. Results: Bronchoalveolar lavage had a high level of cellular infiltrate in immunised animals (420%) compared with control animals; IRI significantly reduced this infiltration (52%). Macrophages from animals immunised and challenged with OVA presented a 10x increase in phagocytic capacity compared to the control group, whereas immunised animals subjected to IRI showed a reduction in the phagocytic index of 68%. the killing of Klebsiella pneumoniae by macrophages from immunised animals was higher (56%) compared with the control group but reduced in animals submitted to IRI (45%). Immunised and challenged group showed an increase in gene expression levels of IL-10(450%), HO-1 (259%), INF-gamma (460%) and MCP-1 (370%) compared to the immunised group subjected to IRI. Conclusions: Renal ischaemia and reperfusion injury apparently alters the phagocytic and microbicidal capacity of macrophages, reducing lung inflammation to OVA. Copyright (C) 2013 S. Karger AG, Basel
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spelling Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar MacrophagesAlveolar macrophagesPulmonary inflammationKillingKlebsiella pneumoniaIschaemia and reperfusion injuryBackground/Aims: Renal ischaemia-reperfusion injury (IRI) is a systemic inflammatory process in which Th1 responses predominate affecting other organs including the lungs. the present study explored the phagocytic and microbicidal capacity of macrophages in rats with lung inflammation that underwent IRI. Methods: the alveolar macrophages of rats sensitised to OVA were evaluated for phagocytosis and bacterial killing 24h after antigen challenge in animals with or without prior submission to 60 min of renal ischaemia. Results: Bronchoalveolar lavage had a high level of cellular infiltrate in immunised animals (420%) compared with control animals; IRI significantly reduced this infiltration (52%). Macrophages from animals immunised and challenged with OVA presented a 10x increase in phagocytic capacity compared to the control group, whereas immunised animals subjected to IRI showed a reduction in the phagocytic index of 68%. the killing of Klebsiella pneumoniae by macrophages from immunised animals was higher (56%) compared with the control group but reduced in animals submitted to IRI (45%). Immunised and challenged group showed an increase in gene expression levels of IL-10(450%), HO-1 (259%), INF-gamma (460%) and MCP-1 (370%) compared to the immunised group subjected to IRI. Conclusions: Renal ischaemia and reperfusion injury apparently alters the phagocytic and microbicidal capacity of macrophages, reducing lung inflammation to OVA. Copyright (C) 2013 S. Karger AG, BaselUniversidade Federal de São Paulo, Disciplina Nefrol, Lab Imunol Clin & Expt, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Inflamacao & Farmacol Vasc, BR-04023900 São Paulo, BrazilUniv São Paulo, Inst Ciencias Biomed, Dept Imunol, Lab Imunobiol Transplante, BR-05508 São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Nefrol, Lab Imunol Clin & Expt, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Inflamacao & Farmacol Vasc, BR-04023900 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)INCT Complex FluidsFADA-UNIFESPFAPESP: 07/07139-3FAPESP: 10/52180-4FAPESP: 10/01404-0FAPESP: 12/02270-0FAPESP: 12/51104-8KargerUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Silva, Reinaldo Correia [UNIFESP]Landgraf, Maristella de Almeida [UNIFESP]Correa-Costa, MatheusSemedo, Patricia [UNIFESP]Cenedeze, Marcos Antonio [UNIFESP]Pacheco-Silva, Alvaro [UNIFESP]Landgraf, Richardt Gama [UNIFESP]Câmara, Niels Olsen Saraiva [UNIFESP]2016-01-24T14:30:55Z2016-01-24T14:30:55Z2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion179-188application/pdfhttp://dx.doi.org/10.1159/000343359Cellular Physiology and Biochemistry. Basel: Karger, v. 31, n. 2-3, p. 179-188, 2013.10.1159/000343359WOS000318411800001.pdf1015-8987http://repositorio.unifesp.br/handle/11600/35715WOS:000318411800001engCellular Physiology and Biochemistryinfo:eu-repo/semantics/openAccesshttp://www.karger.com/Services/RightsPermissionsreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-01T04:28:34Zoai:repositorio.unifesp.br/:11600/35715Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-01T04:28:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages
title Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages
spellingShingle Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages
Silva, Reinaldo Correia [UNIFESP]
Alveolar macrophages
Pulmonary inflammation
Killing
Klebsiella pneumonia
Ischaemia and reperfusion injury
title_short Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages
title_full Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages
title_fullStr Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages
title_full_unstemmed Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages
title_sort Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages
author Silva, Reinaldo Correia [UNIFESP]
author_facet Silva, Reinaldo Correia [UNIFESP]
Landgraf, Maristella de Almeida [UNIFESP]
Correa-Costa, Matheus
Semedo, Patricia [UNIFESP]
Cenedeze, Marcos Antonio [UNIFESP]
Pacheco-Silva, Alvaro [UNIFESP]
Landgraf, Richardt Gama [UNIFESP]
Câmara, Niels Olsen Saraiva [UNIFESP]
author_role author
author2 Landgraf, Maristella de Almeida [UNIFESP]
Correa-Costa, Matheus
Semedo, Patricia [UNIFESP]
Cenedeze, Marcos Antonio [UNIFESP]
Pacheco-Silva, Alvaro [UNIFESP]
Landgraf, Richardt Gama [UNIFESP]
Câmara, Niels Olsen Saraiva [UNIFESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Silva, Reinaldo Correia [UNIFESP]
Landgraf, Maristella de Almeida [UNIFESP]
Correa-Costa, Matheus
Semedo, Patricia [UNIFESP]
Cenedeze, Marcos Antonio [UNIFESP]
Pacheco-Silva, Alvaro [UNIFESP]
Landgraf, Richardt Gama [UNIFESP]
Câmara, Niels Olsen Saraiva [UNIFESP]
dc.subject.por.fl_str_mv Alveolar macrophages
Pulmonary inflammation
Killing
Klebsiella pneumonia
Ischaemia and reperfusion injury
topic Alveolar macrophages
Pulmonary inflammation
Killing
Klebsiella pneumonia
Ischaemia and reperfusion injury
description Background/Aims: Renal ischaemia-reperfusion injury (IRI) is a systemic inflammatory process in which Th1 responses predominate affecting other organs including the lungs. the present study explored the phagocytic and microbicidal capacity of macrophages in rats with lung inflammation that underwent IRI. Methods: the alveolar macrophages of rats sensitised to OVA were evaluated for phagocytosis and bacterial killing 24h after antigen challenge in animals with or without prior submission to 60 min of renal ischaemia. Results: Bronchoalveolar lavage had a high level of cellular infiltrate in immunised animals (420%) compared with control animals; IRI significantly reduced this infiltration (52%). Macrophages from animals immunised and challenged with OVA presented a 10x increase in phagocytic capacity compared to the control group, whereas immunised animals subjected to IRI showed a reduction in the phagocytic index of 68%. the killing of Klebsiella pneumoniae by macrophages from immunised animals was higher (56%) compared with the control group but reduced in animals submitted to IRI (45%). Immunised and challenged group showed an increase in gene expression levels of IL-10(450%), HO-1 (259%), INF-gamma (460%) and MCP-1 (370%) compared to the immunised group subjected to IRI. Conclusions: Renal ischaemia and reperfusion injury apparently alters the phagocytic and microbicidal capacity of macrophages, reducing lung inflammation to OVA. Copyright (C) 2013 S. Karger AG, Basel
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
2016-01-24T14:30:55Z
2016-01-24T14:30:55Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1159/000343359
Cellular Physiology and Biochemistry. Basel: Karger, v. 31, n. 2-3, p. 179-188, 2013.
10.1159/000343359
WOS000318411800001.pdf
1015-8987
http://repositorio.unifesp.br/handle/11600/35715
WOS:000318411800001
url http://dx.doi.org/10.1159/000343359
http://repositorio.unifesp.br/handle/11600/35715
identifier_str_mv Cellular Physiology and Biochemistry. Basel: Karger, v. 31, n. 2-3, p. 179-188, 2013.
10.1159/000343359
WOS000318411800001.pdf
1015-8987
WOS:000318411800001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cellular Physiology and Biochemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.karger.com/Services/RightsPermissions
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.karger.com/Services/RightsPermissions
dc.format.none.fl_str_mv 179-188
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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