Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1159/000343359 http://repositorio.unifesp.br/handle/11600/35715 |
Resumo: | Background/Aims: Renal ischaemia-reperfusion injury (IRI) is a systemic inflammatory process in which Th1 responses predominate affecting other organs including the lungs. the present study explored the phagocytic and microbicidal capacity of macrophages in rats with lung inflammation that underwent IRI. Methods: the alveolar macrophages of rats sensitised to OVA were evaluated for phagocytosis and bacterial killing 24h after antigen challenge in animals with or without prior submission to 60 min of renal ischaemia. Results: Bronchoalveolar lavage had a high level of cellular infiltrate in immunised animals (420%) compared with control animals; IRI significantly reduced this infiltration (52%). Macrophages from animals immunised and challenged with OVA presented a 10x increase in phagocytic capacity compared to the control group, whereas immunised animals subjected to IRI showed a reduction in the phagocytic index of 68%. the killing of Klebsiella pneumoniae by macrophages from immunised animals was higher (56%) compared with the control group but reduced in animals submitted to IRI (45%). Immunised and challenged group showed an increase in gene expression levels of IL-10(450%), HO-1 (259%), INF-gamma (460%) and MCP-1 (370%) compared to the immunised group subjected to IRI. Conclusions: Renal ischaemia and reperfusion injury apparently alters the phagocytic and microbicidal capacity of macrophages, reducing lung inflammation to OVA. Copyright (C) 2013 S. Karger AG, Basel |
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Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar MacrophagesAlveolar macrophagesPulmonary inflammationKillingKlebsiella pneumoniaIschaemia and reperfusion injuryBackground/Aims: Renal ischaemia-reperfusion injury (IRI) is a systemic inflammatory process in which Th1 responses predominate affecting other organs including the lungs. the present study explored the phagocytic and microbicidal capacity of macrophages in rats with lung inflammation that underwent IRI. Methods: the alveolar macrophages of rats sensitised to OVA were evaluated for phagocytosis and bacterial killing 24h after antigen challenge in animals with or without prior submission to 60 min of renal ischaemia. Results: Bronchoalveolar lavage had a high level of cellular infiltrate in immunised animals (420%) compared with control animals; IRI significantly reduced this infiltration (52%). Macrophages from animals immunised and challenged with OVA presented a 10x increase in phagocytic capacity compared to the control group, whereas immunised animals subjected to IRI showed a reduction in the phagocytic index of 68%. the killing of Klebsiella pneumoniae by macrophages from immunised animals was higher (56%) compared with the control group but reduced in animals submitted to IRI (45%). Immunised and challenged group showed an increase in gene expression levels of IL-10(450%), HO-1 (259%), INF-gamma (460%) and MCP-1 (370%) compared to the immunised group subjected to IRI. Conclusions: Renal ischaemia and reperfusion injury apparently alters the phagocytic and microbicidal capacity of macrophages, reducing lung inflammation to OVA. Copyright (C) 2013 S. Karger AG, BaselUniversidade Federal de São Paulo, Disciplina Nefrol, Lab Imunol Clin & Expt, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Inflamacao & Farmacol Vasc, BR-04023900 São Paulo, BrazilUniv São Paulo, Inst Ciencias Biomed, Dept Imunol, Lab Imunobiol Transplante, BR-05508 São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Nefrol, Lab Imunol Clin & Expt, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Lab Inflamacao & Farmacol Vasc, BR-04023900 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)INCT Complex FluidsFADA-UNIFESPFAPESP: 07/07139-3FAPESP: 10/52180-4FAPESP: 10/01404-0FAPESP: 12/02270-0FAPESP: 12/51104-8KargerUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)Silva, Reinaldo Correia [UNIFESP]Landgraf, Maristella de Almeida [UNIFESP]Correa-Costa, MatheusSemedo, Patricia [UNIFESP]Cenedeze, Marcos Antonio [UNIFESP]Pacheco-Silva, Alvaro [UNIFESP]Landgraf, Richardt Gama [UNIFESP]Câmara, Niels Olsen Saraiva [UNIFESP]2016-01-24T14:30:55Z2016-01-24T14:30:55Z2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion179-188application/pdfhttp://dx.doi.org/10.1159/000343359Cellular Physiology and Biochemistry. Basel: Karger, v. 31, n. 2-3, p. 179-188, 2013.10.1159/000343359WOS000318411800001.pdf1015-8987http://repositorio.unifesp.br/handle/11600/35715WOS:000318411800001engCellular Physiology and Biochemistryinfo:eu-repo/semantics/openAccesshttp://www.karger.com/Services/RightsPermissionsreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-01T04:28:34Zoai:repositorio.unifesp.br/:11600/35715Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-01T04:28:34Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages |
title |
Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages |
spellingShingle |
Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages Silva, Reinaldo Correia [UNIFESP] Alveolar macrophages Pulmonary inflammation Killing Klebsiella pneumonia Ischaemia and reperfusion injury |
title_short |
Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages |
title_full |
Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages |
title_fullStr |
Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages |
title_full_unstemmed |
Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages |
title_sort |
Acute Kidney Injury Reduces Phagocytic and Microbicidal Capacities of Alveolar Macrophages |
author |
Silva, Reinaldo Correia [UNIFESP] |
author_facet |
Silva, Reinaldo Correia [UNIFESP] Landgraf, Maristella de Almeida [UNIFESP] Correa-Costa, Matheus Semedo, Patricia [UNIFESP] Cenedeze, Marcos Antonio [UNIFESP] Pacheco-Silva, Alvaro [UNIFESP] Landgraf, Richardt Gama [UNIFESP] Câmara, Niels Olsen Saraiva [UNIFESP] |
author_role |
author |
author2 |
Landgraf, Maristella de Almeida [UNIFESP] Correa-Costa, Matheus Semedo, Patricia [UNIFESP] Cenedeze, Marcos Antonio [UNIFESP] Pacheco-Silva, Alvaro [UNIFESP] Landgraf, Richardt Gama [UNIFESP] Câmara, Niels Olsen Saraiva [UNIFESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Silva, Reinaldo Correia [UNIFESP] Landgraf, Maristella de Almeida [UNIFESP] Correa-Costa, Matheus Semedo, Patricia [UNIFESP] Cenedeze, Marcos Antonio [UNIFESP] Pacheco-Silva, Alvaro [UNIFESP] Landgraf, Richardt Gama [UNIFESP] Câmara, Niels Olsen Saraiva [UNIFESP] |
dc.subject.por.fl_str_mv |
Alveolar macrophages Pulmonary inflammation Killing Klebsiella pneumonia Ischaemia and reperfusion injury |
topic |
Alveolar macrophages Pulmonary inflammation Killing Klebsiella pneumonia Ischaemia and reperfusion injury |
description |
Background/Aims: Renal ischaemia-reperfusion injury (IRI) is a systemic inflammatory process in which Th1 responses predominate affecting other organs including the lungs. the present study explored the phagocytic and microbicidal capacity of macrophages in rats with lung inflammation that underwent IRI. Methods: the alveolar macrophages of rats sensitised to OVA were evaluated for phagocytosis and bacterial killing 24h after antigen challenge in animals with or without prior submission to 60 min of renal ischaemia. Results: Bronchoalveolar lavage had a high level of cellular infiltrate in immunised animals (420%) compared with control animals; IRI significantly reduced this infiltration (52%). Macrophages from animals immunised and challenged with OVA presented a 10x increase in phagocytic capacity compared to the control group, whereas immunised animals subjected to IRI showed a reduction in the phagocytic index of 68%. the killing of Klebsiella pneumoniae by macrophages from immunised animals was higher (56%) compared with the control group but reduced in animals submitted to IRI (45%). Immunised and challenged group showed an increase in gene expression levels of IL-10(450%), HO-1 (259%), INF-gamma (460%) and MCP-1 (370%) compared to the immunised group subjected to IRI. Conclusions: Renal ischaemia and reperfusion injury apparently alters the phagocytic and microbicidal capacity of macrophages, reducing lung inflammation to OVA. Copyright (C) 2013 S. Karger AG, Basel |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 2016-01-24T14:30:55Z 2016-01-24T14:30:55Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1159/000343359 Cellular Physiology and Biochemistry. Basel: Karger, v. 31, n. 2-3, p. 179-188, 2013. 10.1159/000343359 WOS000318411800001.pdf 1015-8987 http://repositorio.unifesp.br/handle/11600/35715 WOS:000318411800001 |
url |
http://dx.doi.org/10.1159/000343359 http://repositorio.unifesp.br/handle/11600/35715 |
identifier_str_mv |
Cellular Physiology and Biochemistry. Basel: Karger, v. 31, n. 2-3, p. 179-188, 2013. 10.1159/000343359 WOS000318411800001.pdf 1015-8987 WOS:000318411800001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cellular Physiology and Biochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess http://www.karger.com/Services/RightsPermissions |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://www.karger.com/Services/RightsPermissions |
dc.format.none.fl_str_mv |
179-188 application/pdf |
dc.publisher.none.fl_str_mv |
Karger |
publisher.none.fl_str_mv |
Karger |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268400491298816 |