Erythropoietin reduces the expression of myostatin in mdx dystrophic mice
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/38368 http://dx.doi.org/10.1590/1414-431X20143858 |
Resumo: | Erythropoietin (EPO) has been well characterized as a renal glycoprotein hormone regulating red blood cell production by inhibiting apoptosis of erythrocyte progenitors in hematopoietic tissues. EPO exerts regulatory effects in cardiac and skeletal muscles. Duchenne muscular dystrophy is a lethal degenerative disorder of skeletal and cardiac muscle. in this study, we tested the possible therapeutic beneficial effect of recombinant EPO (rhEPO) in dystrophic muscles in mdx mice. Total strength was measured using a force transducer coupled to a computer. Gene expression for myostatin, transforming growth factor-beta 1 (TGF-beta 1), and tumor necrosis factor-alpha (TNF-alpha) was determined by quantitative real time polymerase chain reaction. Myostatin expression was significantly decreased in quadriceps from mdx mice treated with rhEPO (rhEPO = 0.60 +/- 0.11, control= 1.07 +/- 0.11). On the other hand, rhEPO had no significant effect on the expression of TGF-beta 1 (rhEPO = 0.95 +/- 0.14, control= 1.05 +/- 0.16) and TNF-alpha (rhEPO = 0.73 +/- 0.20, control= 1.01 +/- 0.09). These results may help to clarify some of the direct actions of EPO on skeletal muscle. |
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Feder, DavidRugollini, M.Santomauro, A.Oliveira, Luciano P.Lioi, V. P.Santos, Rosangela Aparecida dosFerreira, Leonardo G.Nunes, Maria TerezaCarvalho, Maria HelenaDelgado, Pilar O.Carvalho, Alzira A. S.Fonseca, Fernando Luiz Affonso [UNIFESP]Fac Med ABCUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)2016-01-24T14:38:03Z2016-01-24T14:38:03Z2014-11-01Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 47, n. 11, p. 966-971, 2014.0100-879Xhttp://repositorio.unifesp.br/handle/11600/38368http://dx.doi.org/10.1590/1414-431X20143858S0100-879X2014001100966.pdfS0100-879X201400110096610.1590/1414-431X20143858WOS:000343748800006Erythropoietin (EPO) has been well characterized as a renal glycoprotein hormone regulating red blood cell production by inhibiting apoptosis of erythrocyte progenitors in hematopoietic tissues. EPO exerts regulatory effects in cardiac and skeletal muscles. Duchenne muscular dystrophy is a lethal degenerative disorder of skeletal and cardiac muscle. in this study, we tested the possible therapeutic beneficial effect of recombinant EPO (rhEPO) in dystrophic muscles in mdx mice. Total strength was measured using a force transducer coupled to a computer. Gene expression for myostatin, transforming growth factor-beta 1 (TGF-beta 1), and tumor necrosis factor-alpha (TNF-alpha) was determined by quantitative real time polymerase chain reaction. Myostatin expression was significantly decreased in quadriceps from mdx mice treated with rhEPO (rhEPO = 0.60 +/- 0.11, control= 1.07 +/- 0.11). On the other hand, rhEPO had no significant effect on the expression of TGF-beta 1 (rhEPO = 0.95 +/- 0.14, control= 1.05 +/- 0.16) and TNF-alpha (rhEPO = 0.73 +/- 0.20, control= 1.01 +/- 0.09). These results may help to clarify some of the direct actions of EPO on skeletal muscle.Fac Med ABC, BR-09060650 Santo Andre, SP, BrazilUniv São Paulo, Inst Ciencias Biomed, BR-05508 São Paulo, BrazilUniversidade Federal de São Paulo, Inst Ciencias Quim Ambientais & Farmaceut, Diadema, SP, BrazilUniversidade Federal de São Paulo, Inst Ciencias Quim Ambientais & Farmaceut, Diadema, SP, BrazilWeb of Science966-971engAssoc Bras Divulg CientificaBrazilian Journal of Medical and Biological ResearchMuscular dystrophyErythropoietinMyostatinSkeletal muscleQuadricepsErythropoietin reduces the expression of myostatin in mdx dystrophic miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0100-879X2014001100966.pdfapplication/pdf162945${dspace.ui.url}/bitstream/11600/38368/1/S0100-879X2014001100966.pdf283d825729b234ded272cc93bd8eeb04MD51open accessTEXTS0100-879X2014001100966.pdf.txtS0100-879X2014001100966.pdf.txtExtracted texttext/plain26145${dspace.ui.url}/bitstream/11600/38368/8/S0100-879X2014001100966.pdf.txt03ce710432becc1567482e4e98acaeb7MD58open accessTHUMBNAILS0100-879X2014001100966.pdf.jpgS0100-879X2014001100966.pdf.jpgIM Thumbnailimage/jpeg6480${dspace.ui.url}/bitstream/11600/38368/10/S0100-879X2014001100966.pdf.jpg2fb4a84730ae75f150efe123b5c2231eMD510open access11600/383682023-06-05 19:23:00.373open accessoai:repositorio.unifesp.br:11600/38368Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:23Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Erythropoietin reduces the expression of myostatin in mdx dystrophic mice |
title |
Erythropoietin reduces the expression of myostatin in mdx dystrophic mice |
spellingShingle |
Erythropoietin reduces the expression of myostatin in mdx dystrophic mice Feder, David Muscular dystrophy Erythropoietin Myostatin Skeletal muscle Quadriceps |
title_short |
Erythropoietin reduces the expression of myostatin in mdx dystrophic mice |
title_full |
Erythropoietin reduces the expression of myostatin in mdx dystrophic mice |
title_fullStr |
Erythropoietin reduces the expression of myostatin in mdx dystrophic mice |
title_full_unstemmed |
Erythropoietin reduces the expression of myostatin in mdx dystrophic mice |
title_sort |
Erythropoietin reduces the expression of myostatin in mdx dystrophic mice |
author |
Feder, David |
author_facet |
Feder, David Rugollini, M. Santomauro, A. Oliveira, Luciano P. Lioi, V. P. Santos, Rosangela Aparecida dos Ferreira, Leonardo G. Nunes, Maria Tereza Carvalho, Maria Helena Delgado, Pilar O. Carvalho, Alzira A. S. Fonseca, Fernando Luiz Affonso [UNIFESP] |
author_role |
author |
author2 |
Rugollini, M. Santomauro, A. Oliveira, Luciano P. Lioi, V. P. Santos, Rosangela Aparecida dos Ferreira, Leonardo G. Nunes, Maria Tereza Carvalho, Maria Helena Delgado, Pilar O. Carvalho, Alzira A. S. Fonseca, Fernando Luiz Affonso [UNIFESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Fac Med ABC Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Feder, David Rugollini, M. Santomauro, A. Oliveira, Luciano P. Lioi, V. P. Santos, Rosangela Aparecida dos Ferreira, Leonardo G. Nunes, Maria Tereza Carvalho, Maria Helena Delgado, Pilar O. Carvalho, Alzira A. S. Fonseca, Fernando Luiz Affonso [UNIFESP] |
dc.subject.eng.fl_str_mv |
Muscular dystrophy Erythropoietin Myostatin Skeletal muscle Quadriceps |
topic |
Muscular dystrophy Erythropoietin Myostatin Skeletal muscle Quadriceps |
description |
Erythropoietin (EPO) has been well characterized as a renal glycoprotein hormone regulating red blood cell production by inhibiting apoptosis of erythrocyte progenitors in hematopoietic tissues. EPO exerts regulatory effects in cardiac and skeletal muscles. Duchenne muscular dystrophy is a lethal degenerative disorder of skeletal and cardiac muscle. in this study, we tested the possible therapeutic beneficial effect of recombinant EPO (rhEPO) in dystrophic muscles in mdx mice. Total strength was measured using a force transducer coupled to a computer. Gene expression for myostatin, transforming growth factor-beta 1 (TGF-beta 1), and tumor necrosis factor-alpha (TNF-alpha) was determined by quantitative real time polymerase chain reaction. Myostatin expression was significantly decreased in quadriceps from mdx mice treated with rhEPO (rhEPO = 0.60 +/- 0.11, control= 1.07 +/- 0.11). On the other hand, rhEPO had no significant effect on the expression of TGF-beta 1 (rhEPO = 0.95 +/- 0.14, control= 1.05 +/- 0.16) and TNF-alpha (rhEPO = 0.73 +/- 0.20, control= 1.01 +/- 0.09). These results may help to clarify some of the direct actions of EPO on skeletal muscle. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-11-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T14:38:03Z |
dc.date.available.fl_str_mv |
2016-01-24T14:38:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 47, n. 11, p. 966-971, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/38368 http://dx.doi.org/10.1590/1414-431X20143858 |
dc.identifier.issn.none.fl_str_mv |
0100-879X |
dc.identifier.file.none.fl_str_mv |
S0100-879X2014001100966.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0100-879X2014001100966 |
dc.identifier.doi.none.fl_str_mv |
10.1590/1414-431X20143858 |
dc.identifier.wos.none.fl_str_mv |
WOS:000343748800006 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. São Paulo: Assoc Bras Divulg Cientifica, v. 47, n. 11, p. 966-971, 2014. 0100-879X S0100-879X2014001100966.pdf S0100-879X2014001100966 10.1590/1414-431X20143858 WOS:000343748800006 |
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http://repositorio.unifesp.br/handle/11600/38368 http://dx.doi.org/10.1590/1414-431X20143858 |
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Brazilian Journal of Medical and Biological Research |
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Assoc Bras Divulg Cientifica |
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Assoc Bras Divulg Cientifica |
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