The use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitals
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/1949 http://dx.doi.org/10.1590/S1413-86702003000600002 |
Resumo: | Antimicrobial resistance has increased rapidly in Brazil and worldwide during the past few years, giving rise to a growing necessity for antimicrobial resistance surveillance programs. These programs have been instituted in order to monitor bacterial resistance in various regions, and to guide empirical antimicrobial therapy. We evaluated the use of molecular typing in multicenter surveillance programs. We also studied the dissemination modes of selected resistance profiles. Antimicrobial susceptibility to various antimicrobial agents was evaluated by the reference broth microdilution method. Bacterial isolates with selected susceptibility patterns were characterized by pulsed field-gel electrophoresis (PFGE). A total of 119 Gram-negative bacteria were molecularly typed, including 22 imipenem-resistant Pseudomonas aeruginosa, 26 ESBL-producing Escherichia coli, 27 cefoxitin-resistant-ESBL-producing Klebsiella pneumoniae, 33 Enterobacter spp., 8 Citrobacter spp., and 3 S. marcescens isolates resistant to ceftazidime. The isolates were from clinically apparent bacteremia of patients hospitalized in medical centers located in 13 cities of 11 Brazilian states. Our molecular typing results revealed a great genetic diversity among isolates of the same species. However, some major PFGE patterns were found in more than one isolate. All repeated PFGE patterns were detected in only 2 isolates, which were isolated within the same institutions or in different medical centers. We conclude that the ability to characterize organisms phenotypically and genotypically is a powerful epidemiologic tool and it provides unique information that is very important for multicenter surveillance programs. |
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Tosin, Iraci [UNIFESP]Silbert, Suzane [UNIFESP]Sader, Helio Silva [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Federal University of Santa Catarina Centre of Biological Sciences2015-06-14T13:30:14Z2015-06-14T13:30:14Z2003-12-01Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 7, n. 6, p. 360-369, 2003.1413-8670http://repositorio.unifesp.br/handle/11600/1949http://dx.doi.org/10.1590/S1413-86702003000600002S1413-86702003000600002.pdfS1413-8670200300060000210.1590/S1413-86702003000600002Antimicrobial resistance has increased rapidly in Brazil and worldwide during the past few years, giving rise to a growing necessity for antimicrobial resistance surveillance programs. These programs have been instituted in order to monitor bacterial resistance in various regions, and to guide empirical antimicrobial therapy. We evaluated the use of molecular typing in multicenter surveillance programs. We also studied the dissemination modes of selected resistance profiles. Antimicrobial susceptibility to various antimicrobial agents was evaluated by the reference broth microdilution method. Bacterial isolates with selected susceptibility patterns were characterized by pulsed field-gel electrophoresis (PFGE). A total of 119 Gram-negative bacteria were molecularly typed, including 22 imipenem-resistant Pseudomonas aeruginosa, 26 ESBL-producing Escherichia coli, 27 cefoxitin-resistant-ESBL-producing Klebsiella pneumoniae, 33 Enterobacter spp., 8 Citrobacter spp., and 3 S. marcescens isolates resistant to ceftazidime. The isolates were from clinically apparent bacteremia of patients hospitalized in medical centers located in 13 cities of 11 Brazilian states. Our molecular typing results revealed a great genetic diversity among isolates of the same species. However, some major PFGE patterns were found in more than one isolate. All repeated PFGE patterns were detected in only 2 isolates, which were isolated within the same institutions or in different medical centers. We conclude that the ability to characterize organisms phenotypically and genotypically is a powerful epidemiologic tool and it provides unique information that is very important for multicenter surveillance programs.Federal University of São Paulo Special Laboratory of Clinical MicrobiologyFederal University of Santa Catarina Centre of Biological SciencesUNIFESP, Special Laboratory of Clinical MicrobiologySciELO360-369engBrazilian Society of Infectious DiseasesBrazilian Journal of Infectious DiseasesMolecular typingpulsed-field gel electrophoresisantimicrobial resistancecarbapenem-resistant Pseudomonas aeruginosaclonal disseminationSentinela ProgramThe use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitalsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1413-86702003000600002.pdfapplication/pdf863921${dspace.ui.url}/bitstream/11600/1949/1/S1413-86702003000600002.pdfca3b25942373aee992072acfc3f92f14MD51open accessTEXTS1413-86702003000600002.pdf.txtS1413-86702003000600002.pdf.txtExtracted texttext/plain24860${dspace.ui.url}/bitstream/11600/1949/2/S1413-86702003000600002.pdf.txtb9039f282f95d1e782ed17d92dced372MD52open access11600/19492021-10-04 21:20:47.462open accessoai:repositorio.unifesp.br:11600/1949Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-10-05T00:20:47Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
The use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitals |
title |
The use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitals |
spellingShingle |
The use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitals Tosin, Iraci [UNIFESP] Molecular typing pulsed-field gel electrophoresis antimicrobial resistance carbapenem-resistant Pseudomonas aeruginosa clonal dissemination Sentinela Program |
title_short |
The use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitals |
title_full |
The use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitals |
title_fullStr |
The use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitals |
title_full_unstemmed |
The use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitals |
title_sort |
The use of molecular typing to evaluate the dissemination of antimicrobial resistance among gram-negative rods in Brazilian hospitals |
author |
Tosin, Iraci [UNIFESP] |
author_facet |
Tosin, Iraci [UNIFESP] Silbert, Suzane [UNIFESP] Sader, Helio Silva [UNIFESP] |
author_role |
author |
author2 |
Silbert, Suzane [UNIFESP] Sader, Helio Silva [UNIFESP] |
author2_role |
author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Federal University of Santa Catarina Centre of Biological Sciences |
dc.contributor.author.fl_str_mv |
Tosin, Iraci [UNIFESP] Silbert, Suzane [UNIFESP] Sader, Helio Silva [UNIFESP] |
dc.subject.eng.fl_str_mv |
Molecular typing pulsed-field gel electrophoresis antimicrobial resistance carbapenem-resistant Pseudomonas aeruginosa clonal dissemination Sentinela Program |
topic |
Molecular typing pulsed-field gel electrophoresis antimicrobial resistance carbapenem-resistant Pseudomonas aeruginosa clonal dissemination Sentinela Program |
description |
Antimicrobial resistance has increased rapidly in Brazil and worldwide during the past few years, giving rise to a growing necessity for antimicrobial resistance surveillance programs. These programs have been instituted in order to monitor bacterial resistance in various regions, and to guide empirical antimicrobial therapy. We evaluated the use of molecular typing in multicenter surveillance programs. We also studied the dissemination modes of selected resistance profiles. Antimicrobial susceptibility to various antimicrobial agents was evaluated by the reference broth microdilution method. Bacterial isolates with selected susceptibility patterns were characterized by pulsed field-gel electrophoresis (PFGE). A total of 119 Gram-negative bacteria were molecularly typed, including 22 imipenem-resistant Pseudomonas aeruginosa, 26 ESBL-producing Escherichia coli, 27 cefoxitin-resistant-ESBL-producing Klebsiella pneumoniae, 33 Enterobacter spp., 8 Citrobacter spp., and 3 S. marcescens isolates resistant to ceftazidime. The isolates were from clinically apparent bacteremia of patients hospitalized in medical centers located in 13 cities of 11 Brazilian states. Our molecular typing results revealed a great genetic diversity among isolates of the same species. However, some major PFGE patterns were found in more than one isolate. All repeated PFGE patterns were detected in only 2 isolates, which were isolated within the same institutions or in different medical centers. We conclude that the ability to characterize organisms phenotypically and genotypically is a powerful epidemiologic tool and it provides unique information that is very important for multicenter surveillance programs. |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003-12-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:30:14Z |
dc.date.available.fl_str_mv |
2015-06-14T13:30:14Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 7, n. 6, p. 360-369, 2003. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/1949 http://dx.doi.org/10.1590/S1413-86702003000600002 |
dc.identifier.issn.none.fl_str_mv |
1413-8670 |
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S1413-86702003000600002.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1413-86702003000600002 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1413-86702003000600002 |
identifier_str_mv |
Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 7, n. 6, p. 360-369, 2003. 1413-8670 S1413-86702003000600002.pdf S1413-86702003000600002 10.1590/S1413-86702003000600002 |
url |
http://repositorio.unifesp.br/handle/11600/1949 http://dx.doi.org/10.1590/S1413-86702003000600002 |
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eng |
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360-369 |
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Brazilian Society of Infectious Diseases |
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Brazilian Society of Infectious Diseases |
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