Protective role of mitochondrial unsaturated lipids on the preservation of the apoptotic ability of cytochrome c exposed to singlet oxygen
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/29969 http://dx.doi.org/10.1074/jbc.M700009200 |
Resumo: | Cytochrome c-mediated apoptosis in cells submitted to photodynamic therapy raises the question about the ability of photodynamically oxidized cytochrome c ( cytc405) to trigger apoptosis as well as the effect of membranes on protein photo-oxidation. Cytochrome c was submitted to irradiation in the presence of MB+ in phosphate buffer and in the presence of four types of phosphatidylcholine/phosphatidylethanolamine/cardiolipin ( PCPECL) liposomes ( 50/30/20%): totally saturated lipids ( tsPCPECL), totally unsaturated lipids ( tuPCPECL), partially unsaturated ( 80%) lipids, with unsaturation in the PC and PE content ( puPCPECL80), and partially unsaturated ( 20%) lipids, with unsaturation in the CL content ( puPCPECL20). Cytc405 was formed by irradiation in buffered water and in tsPCPECL and puPCPECL20 liposomes. in the presence of tuPCPECL and puPCPECL80, cytochrome c was protected from photodynamic damage ( lipid-protected cytochrome c). in CL liposomes, 25% unsaturated lipids were enough to protect cytochrome c. the presence of unsaturated lipids, in amounts varying according to the liposome composition, are crucial to protect cytochrome c. Interesting findings corroborating the unsaturated lipids as cytochrome c protectors were obtained from the analysis of the lipid-oxidized derivatives of the samples. Native cytochrome c, lipid-protected cytochrome c, and cytc405 were microinjected in aortic smooth muscle cells. Apoptosis, characterized by nucleus blebbing and chromatin condensation, was detected in cells loaded with native and lipid protected cytochrome c but not in cells loaded with cytc405. These results suggest that photodynamic therapy-promoted apoptosis is feasible due to the protective effect of the mitochondrial lipids on the cytochrome c structure and function. |
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Rodrigues, TiagoFrança, Lucimar Pereira de [UNIFESP]Kawai, CintiaFaria, Priscila A. deMugnol, Katia Cristina Ugolini [UNIFESP]Braga, Fernanda M.Tersariol, Ivarne Luis dos Santos [UNIFESP]Smaili, Soraya Soubhi [UNIFESP]Nantes, Iseli L.Univ Mogi das CruzesUniversidade Federal de São Paulo (UNIFESP)2016-01-24T13:49:00Z2016-01-24T13:49:00Z2007-08-31Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 282, n. 35, p. 25577-25587, 2007.0021-9258http://repositorio.unifesp.br/handle/11600/29969http://dx.doi.org/10.1074/jbc.M70000920010.1074/jbc.M700009200WOS:000249014100044Cytochrome c-mediated apoptosis in cells submitted to photodynamic therapy raises the question about the ability of photodynamically oxidized cytochrome c ( cytc405) to trigger apoptosis as well as the effect of membranes on protein photo-oxidation. Cytochrome c was submitted to irradiation in the presence of MB+ in phosphate buffer and in the presence of four types of phosphatidylcholine/phosphatidylethanolamine/cardiolipin ( PCPECL) liposomes ( 50/30/20%): totally saturated lipids ( tsPCPECL), totally unsaturated lipids ( tuPCPECL), partially unsaturated ( 80%) lipids, with unsaturation in the PC and PE content ( puPCPECL80), and partially unsaturated ( 20%) lipids, with unsaturation in the CL content ( puPCPECL20). Cytc405 was formed by irradiation in buffered water and in tsPCPECL and puPCPECL20 liposomes. in the presence of tuPCPECL and puPCPECL80, cytochrome c was protected from photodynamic damage ( lipid-protected cytochrome c). in CL liposomes, 25% unsaturated lipids were enough to protect cytochrome c. the presence of unsaturated lipids, in amounts varying according to the liposome composition, are crucial to protect cytochrome c. Interesting findings corroborating the unsaturated lipids as cytochrome c protectors were obtained from the analysis of the lipid-oxidized derivatives of the samples. Native cytochrome c, lipid-protected cytochrome c, and cytc405 were microinjected in aortic smooth muscle cells. Apoptosis, characterized by nucleus blebbing and chromatin condensation, was detected in cells loaded with native and lipid protected cytochrome c but not in cells loaded with cytc405. These results suggest that photodynamic therapy-promoted apoptosis is feasible due to the protective effect of the mitochondrial lipids on the cytochrome c structure and function.Univ Mogi das Cruzes, Ctr Interdisciplinar Invest Bioquim, BR-08780911 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Farmacol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Farmacol, São Paulo, BrazilWeb of Science25577-25587engAmer Soc Biochemistry Molecular Biology IncJournal of Biological ChemistryProtective role of mitochondrial unsaturated lipids on the preservation of the apoptotic ability of cytochrome c exposed to singlet oxygeninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/299692022-11-03 10:40:50.56metadata only accessoai:repositorio.unifesp.br:11600/29969Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-11-03T13:40:50Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Protective role of mitochondrial unsaturated lipids on the preservation of the apoptotic ability of cytochrome c exposed to singlet oxygen |
title |
Protective role of mitochondrial unsaturated lipids on the preservation of the apoptotic ability of cytochrome c exposed to singlet oxygen |
spellingShingle |
Protective role of mitochondrial unsaturated lipids on the preservation of the apoptotic ability of cytochrome c exposed to singlet oxygen Rodrigues, Tiago |
title_short |
Protective role of mitochondrial unsaturated lipids on the preservation of the apoptotic ability of cytochrome c exposed to singlet oxygen |
title_full |
Protective role of mitochondrial unsaturated lipids on the preservation of the apoptotic ability of cytochrome c exposed to singlet oxygen |
title_fullStr |
Protective role of mitochondrial unsaturated lipids on the preservation of the apoptotic ability of cytochrome c exposed to singlet oxygen |
title_full_unstemmed |
Protective role of mitochondrial unsaturated lipids on the preservation of the apoptotic ability of cytochrome c exposed to singlet oxygen |
title_sort |
Protective role of mitochondrial unsaturated lipids on the preservation of the apoptotic ability of cytochrome c exposed to singlet oxygen |
author |
Rodrigues, Tiago |
author_facet |
Rodrigues, Tiago França, Lucimar Pereira de [UNIFESP] Kawai, Cintia Faria, Priscila A. de Mugnol, Katia Cristina Ugolini [UNIFESP] Braga, Fernanda M. Tersariol, Ivarne Luis dos Santos [UNIFESP] Smaili, Soraya Soubhi [UNIFESP] Nantes, Iseli L. |
author_role |
author |
author2 |
França, Lucimar Pereira de [UNIFESP] Kawai, Cintia Faria, Priscila A. de Mugnol, Katia Cristina Ugolini [UNIFESP] Braga, Fernanda M. Tersariol, Ivarne Luis dos Santos [UNIFESP] Smaili, Soraya Soubhi [UNIFESP] Nantes, Iseli L. |
author2_role |
author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Univ Mogi das Cruzes Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Rodrigues, Tiago França, Lucimar Pereira de [UNIFESP] Kawai, Cintia Faria, Priscila A. de Mugnol, Katia Cristina Ugolini [UNIFESP] Braga, Fernanda M. Tersariol, Ivarne Luis dos Santos [UNIFESP] Smaili, Soraya Soubhi [UNIFESP] Nantes, Iseli L. |
description |
Cytochrome c-mediated apoptosis in cells submitted to photodynamic therapy raises the question about the ability of photodynamically oxidized cytochrome c ( cytc405) to trigger apoptosis as well as the effect of membranes on protein photo-oxidation. Cytochrome c was submitted to irradiation in the presence of MB+ in phosphate buffer and in the presence of four types of phosphatidylcholine/phosphatidylethanolamine/cardiolipin ( PCPECL) liposomes ( 50/30/20%): totally saturated lipids ( tsPCPECL), totally unsaturated lipids ( tuPCPECL), partially unsaturated ( 80%) lipids, with unsaturation in the PC and PE content ( puPCPECL80), and partially unsaturated ( 20%) lipids, with unsaturation in the CL content ( puPCPECL20). Cytc405 was formed by irradiation in buffered water and in tsPCPECL and puPCPECL20 liposomes. in the presence of tuPCPECL and puPCPECL80, cytochrome c was protected from photodynamic damage ( lipid-protected cytochrome c). in CL liposomes, 25% unsaturated lipids were enough to protect cytochrome c. the presence of unsaturated lipids, in amounts varying according to the liposome composition, are crucial to protect cytochrome c. Interesting findings corroborating the unsaturated lipids as cytochrome c protectors were obtained from the analysis of the lipid-oxidized derivatives of the samples. Native cytochrome c, lipid-protected cytochrome c, and cytc405 were microinjected in aortic smooth muscle cells. Apoptosis, characterized by nucleus blebbing and chromatin condensation, was detected in cells loaded with native and lipid protected cytochrome c but not in cells loaded with cytc405. These results suggest that photodynamic therapy-promoted apoptosis is feasible due to the protective effect of the mitochondrial lipids on the cytochrome c structure and function. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-08-31 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:49:00Z |
dc.date.available.fl_str_mv |
2016-01-24T13:49:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 282, n. 35, p. 25577-25587, 2007. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/29969 http://dx.doi.org/10.1074/jbc.M700009200 |
dc.identifier.issn.none.fl_str_mv |
0021-9258 |
dc.identifier.doi.none.fl_str_mv |
10.1074/jbc.M700009200 |
dc.identifier.wos.none.fl_str_mv |
WOS:000249014100044 |
identifier_str_mv |
Journal of Biological Chemistry. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 282, n. 35, p. 25577-25587, 2007. 0021-9258 10.1074/jbc.M700009200 WOS:000249014100044 |
url |
http://repositorio.unifesp.br/handle/11600/29969 http://dx.doi.org/10.1074/jbc.M700009200 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Journal of Biological Chemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
25577-25587 |
dc.publisher.none.fl_str_mv |
Amer Soc Biochemistry Molecular Biology Inc |
publisher.none.fl_str_mv |
Amer Soc Biochemistry Molecular Biology Inc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764178897764352 |