Association of NAD(P)H Oxidase with Glucose-Induced Insulin Secretion by Pancreatic beta-Cells
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/31484 http://dx.doi.org/10.1210/en.2008-1149 |
Resumo: | We previously described the presence of nicotinamide adenine dinucleotide phosphate reduced form [NAD(P)H] oxidase components in pancreatic beta-cells and its activation by glucose, palmitic acid, and proinflammatory cytokines. in the present study, the importance of the NAD(P)H oxidase complex for pancreatic beta-cell function was examined. Rat pancreatic islets were incubated in the presence of glucose plus diphenyleneiodonium, a NAD(P)H oxidase inhibitor, for 1 h or with the antisense oligonucleotide for p47(PHOX) during 24 h. Reactive oxygen species (ROS) production was determined by a fluorescence assay using 2,7-dichlorodihydrofluorescein diacetate. Insulin secretion, intracellular calcium responses, [U-(14)C] glucose oxidation, and expression of glucose transporter-2, glucokinase and insulin genes were examined. Antisense oligonucleotide reduced p47(PHOX) expression [an important NAD(P)H oxidase cytosolic subunit] and similarly to diphenyleneiodonium also blunted the enzyme activity as indicated by reduction of ROS production. Suppression of NAD(P)H oxidase activity had an inhibitory effect on intracellular calcium responses to glucose and glucose-stimulated insulin secretion by isolated islets. NAD(P)H oxidase inhibition also reduced glucose oxidation and gene expression of glucose transporter-2 and glucokinase. These findings indicate that NAD(P)H oxidase activation plays an important role for ROS production by pancreatic beta-cells during glucose-stimulated insulin secretion. the importance of this enzyme complex for the beta-cell metabolism and the machinery involved in insulin secretion were also shown. (Endocrinology 150: 2197-2201, 2009) |
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Morgan, D.Rebelato, E.Abdulkader, F.Graciano, M. F. R.Oliveira-Emilio, H. R.Hirata, Aparecida Emiko [UNIFESP]Rocha, M. S.Bordin, SilvanaCuri, RuiCarpinelli, A. R.Universidade de São Paulo (USP)Univ Estadual Ponta GrossaUniversidade Federal de São Paulo (UNIFESP)2016-01-24T13:52:29Z2016-01-24T13:52:29Z2009-05-01Endocrinology. Chevy Chase: Endocrine Soc, v. 150, n. 5, p. 2197-2201, 2009.0013-7227http://repositorio.unifesp.br/handle/11600/31484http://dx.doi.org/10.1210/en.2008-114910.1210/en.2008-1149WOS:000265407500023We previously described the presence of nicotinamide adenine dinucleotide phosphate reduced form [NAD(P)H] oxidase components in pancreatic beta-cells and its activation by glucose, palmitic acid, and proinflammatory cytokines. in the present study, the importance of the NAD(P)H oxidase complex for pancreatic beta-cell function was examined. Rat pancreatic islets were incubated in the presence of glucose plus diphenyleneiodonium, a NAD(P)H oxidase inhibitor, for 1 h or with the antisense oligonucleotide for p47(PHOX) during 24 h. Reactive oxygen species (ROS) production was determined by a fluorescence assay using 2,7-dichlorodihydrofluorescein diacetate. Insulin secretion, intracellular calcium responses, [U-(14)C] glucose oxidation, and expression of glucose transporter-2, glucokinase and insulin genes were examined. Antisense oligonucleotide reduced p47(PHOX) expression [an important NAD(P)H oxidase cytosolic subunit] and similarly to diphenyleneiodonium also blunted the enzyme activity as indicated by reduction of ROS production. Suppression of NAD(P)H oxidase activity had an inhibitory effect on intracellular calcium responses to glucose and glucose-stimulated insulin secretion by isolated islets. NAD(P)H oxidase inhibition also reduced glucose oxidation and gene expression of glucose transporter-2 and glucokinase. These findings indicate that NAD(P)H oxidase activation plays an important role for ROS production by pancreatic beta-cells during glucose-stimulated insulin secretion. the importance of this enzyme complex for the beta-cell metabolism and the machinery involved in insulin secretion were also shown. (Endocrinology 150: 2197-2201, 2009)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Cientfico e TecnologicoUniv São Paulo, Dept Physiol & Biophys, Inst Biomed Sci, BR-05508900 São Paulo, BrazilUniv Estadual Ponta Grossa, Dept Gen Biol, BR-84010919 Ponta Grossa, BrazilUniversidade Federal de São Paulo, Dept Physiol, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Physiol, BR-04023900 São Paulo, BrazilWeb of Science2197-2201engEndocrine SocEndocrinologyAssociation of NAD(P)H Oxidase with Glucose-Induced Insulin Secretion by Pancreatic beta-Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/314842022-09-27 10:00:01.07metadata only accessoai:repositorio.unifesp.br:11600/31484Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652022-09-27T13:00:01Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Association of NAD(P)H Oxidase with Glucose-Induced Insulin Secretion by Pancreatic beta-Cells |
title |
Association of NAD(P)H Oxidase with Glucose-Induced Insulin Secretion by Pancreatic beta-Cells |
spellingShingle |
Association of NAD(P)H Oxidase with Glucose-Induced Insulin Secretion by Pancreatic beta-Cells Morgan, D. |
title_short |
Association of NAD(P)H Oxidase with Glucose-Induced Insulin Secretion by Pancreatic beta-Cells |
title_full |
Association of NAD(P)H Oxidase with Glucose-Induced Insulin Secretion by Pancreatic beta-Cells |
title_fullStr |
Association of NAD(P)H Oxidase with Glucose-Induced Insulin Secretion by Pancreatic beta-Cells |
title_full_unstemmed |
Association of NAD(P)H Oxidase with Glucose-Induced Insulin Secretion by Pancreatic beta-Cells |
title_sort |
Association of NAD(P)H Oxidase with Glucose-Induced Insulin Secretion by Pancreatic beta-Cells |
author |
Morgan, D. |
author_facet |
Morgan, D. Rebelato, E. Abdulkader, F. Graciano, M. F. R. Oliveira-Emilio, H. R. Hirata, Aparecida Emiko [UNIFESP] Rocha, M. S. Bordin, Silvana Curi, Rui Carpinelli, A. R. |
author_role |
author |
author2 |
Rebelato, E. Abdulkader, F. Graciano, M. F. R. Oliveira-Emilio, H. R. Hirata, Aparecida Emiko [UNIFESP] Rocha, M. S. Bordin, Silvana Curi, Rui Carpinelli, A. R. |
author2_role |
author author author author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade de São Paulo (USP) Univ Estadual Ponta Grossa Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Morgan, D. Rebelato, E. Abdulkader, F. Graciano, M. F. R. Oliveira-Emilio, H. R. Hirata, Aparecida Emiko [UNIFESP] Rocha, M. S. Bordin, Silvana Curi, Rui Carpinelli, A. R. |
description |
We previously described the presence of nicotinamide adenine dinucleotide phosphate reduced form [NAD(P)H] oxidase components in pancreatic beta-cells and its activation by glucose, palmitic acid, and proinflammatory cytokines. in the present study, the importance of the NAD(P)H oxidase complex for pancreatic beta-cell function was examined. Rat pancreatic islets were incubated in the presence of glucose plus diphenyleneiodonium, a NAD(P)H oxidase inhibitor, for 1 h or with the antisense oligonucleotide for p47(PHOX) during 24 h. Reactive oxygen species (ROS) production was determined by a fluorescence assay using 2,7-dichlorodihydrofluorescein diacetate. Insulin secretion, intracellular calcium responses, [U-(14)C] glucose oxidation, and expression of glucose transporter-2, glucokinase and insulin genes were examined. Antisense oligonucleotide reduced p47(PHOX) expression [an important NAD(P)H oxidase cytosolic subunit] and similarly to diphenyleneiodonium also blunted the enzyme activity as indicated by reduction of ROS production. Suppression of NAD(P)H oxidase activity had an inhibitory effect on intracellular calcium responses to glucose and glucose-stimulated insulin secretion by isolated islets. NAD(P)H oxidase inhibition also reduced glucose oxidation and gene expression of glucose transporter-2 and glucokinase. These findings indicate that NAD(P)H oxidase activation plays an important role for ROS production by pancreatic beta-cells during glucose-stimulated insulin secretion. the importance of this enzyme complex for the beta-cell metabolism and the machinery involved in insulin secretion were also shown. (Endocrinology 150: 2197-2201, 2009) |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-05-01 |
dc.date.accessioned.fl_str_mv |
2016-01-24T13:52:29Z |
dc.date.available.fl_str_mv |
2016-01-24T13:52:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Endocrinology. Chevy Chase: Endocrine Soc, v. 150, n. 5, p. 2197-2201, 2009. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/31484 http://dx.doi.org/10.1210/en.2008-1149 |
dc.identifier.issn.none.fl_str_mv |
0013-7227 |
dc.identifier.doi.none.fl_str_mv |
10.1210/en.2008-1149 |
dc.identifier.wos.none.fl_str_mv |
WOS:000265407500023 |
identifier_str_mv |
Endocrinology. Chevy Chase: Endocrine Soc, v. 150, n. 5, p. 2197-2201, 2009. 0013-7227 10.1210/en.2008-1149 WOS:000265407500023 |
url |
http://repositorio.unifesp.br/handle/11600/31484 http://dx.doi.org/10.1210/en.2008-1149 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.none.fl_str_mv |
Endocrinology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2197-2201 |
dc.publisher.none.fl_str_mv |
Endocrine Soc |
publisher.none.fl_str_mv |
Endocrine Soc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764166076825600 |