The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Reis, Flavia Coelho Garcia dos
Data de Publicação: 2006
Outros Autores: Judice, Wagner AS, Juliano, Maria Aparecida [UNIFESP], Juliano, Luiz [UNIFESP], Scharfstein, Julio, Lima, Ana Paula CD
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1111/j.1574-6968.2006.00267.x
http://repositorio.unifesp.br/handle/11600/28927
Resumo: Papain-like cysteine proteases are important for the survival of the flagellated protozoa Trypanosoma cruzi, the causative agent of Chagas' Disease. the lysosomal cysteine protease designated as cruzipain or cruzain, is the archetype of a multigene family of related isoforms. We investigated the substrate specificity of the cruzipain 2 isoform using internally quenched fluorogenic substrates. We found that cruzipain 2 and cruzain differ substantially regarding the specificity in the S-2, S-1(') and S-2(') pockets. Our study indicates that cruzipain 2 has a more restricted specificity than cruzain, suggesting that these isoforms might act on distinct natural substrates.
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spelling The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzicysteine proteasetrypanosomeChagas DiseasespecificityisoformPapain-like cysteine proteases are important for the survival of the flagellated protozoa Trypanosoma cruzi, the causative agent of Chagas' Disease. the lysosomal cysteine protease designated as cruzipain or cruzain, is the archetype of a multigene family of related isoforms. We investigated the substrate specificity of the cruzipain 2 isoform using internally quenched fluorogenic substrates. We found that cruzipain 2 and cruzain differ substantially regarding the specificity in the S-2, S-1(') and S-2(') pockets. Our study indicates that cruzipain 2 has a more restricted specificity than cruzain, suggesting that these isoforms might act on distinct natural substrates.Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21949900 Rio de Janeiro, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, São Paulo, BrazilWeb of ScienceBlackwell PublishingUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Federal de São Paulo (UNIFESP)Reis, Flavia Coelho Garcia dosJudice, Wagner ASJuliano, Maria Aparecida [UNIFESP]Juliano, Luiz [UNIFESP]Scharfstein, JulioLima, Ana Paula CD2016-01-24T12:41:12Z2016-01-24T12:41:12Z2006-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion215-220http://dx.doi.org/10.1111/j.1574-6968.2006.00267.xFems Microbiology Letters. Oxford: Blackwell Publishing, v. 259, n. 2, p. 215-220, 2006.10.1111/j.1574-6968.2006.00267.x0378-1097http://repositorio.unifesp.br/handle/11600/28927WOS:000237739500008engFems Microbiology Lettersinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2023-05-18T13:49:26Zoai:repositorio.unifesp.br/:11600/28927Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652023-05-18T13:49:26Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi
title The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi
spellingShingle The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi
Reis, Flavia Coelho Garcia dos
cysteine protease
trypanosome
Chagas Disease
specificity
isoform
title_short The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi
title_full The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi
title_fullStr The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi
title_full_unstemmed The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi
title_sort The substrate specificity of cruzipain 2, a cysteine protease isoform from Trypanosoma cruzi
author Reis, Flavia Coelho Garcia dos
author_facet Reis, Flavia Coelho Garcia dos
Judice, Wagner AS
Juliano, Maria Aparecida [UNIFESP]
Juliano, Luiz [UNIFESP]
Scharfstein, Julio
Lima, Ana Paula CD
author_role author
author2 Judice, Wagner AS
Juliano, Maria Aparecida [UNIFESP]
Juliano, Luiz [UNIFESP]
Scharfstein, Julio
Lima, Ana Paula CD
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Reis, Flavia Coelho Garcia dos
Judice, Wagner AS
Juliano, Maria Aparecida [UNIFESP]
Juliano, Luiz [UNIFESP]
Scharfstein, Julio
Lima, Ana Paula CD
dc.subject.por.fl_str_mv cysteine protease
trypanosome
Chagas Disease
specificity
isoform
topic cysteine protease
trypanosome
Chagas Disease
specificity
isoform
description Papain-like cysteine proteases are important for the survival of the flagellated protozoa Trypanosoma cruzi, the causative agent of Chagas' Disease. the lysosomal cysteine protease designated as cruzipain or cruzain, is the archetype of a multigene family of related isoforms. We investigated the substrate specificity of the cruzipain 2 isoform using internally quenched fluorogenic substrates. We found that cruzipain 2 and cruzain differ substantially regarding the specificity in the S-2, S-1(') and S-2(') pockets. Our study indicates that cruzipain 2 has a more restricted specificity than cruzain, suggesting that these isoforms might act on distinct natural substrates.
publishDate 2006
dc.date.none.fl_str_mv 2006-06-01
2016-01-24T12:41:12Z
2016-01-24T12:41:12Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/j.1574-6968.2006.00267.x
Fems Microbiology Letters. Oxford: Blackwell Publishing, v. 259, n. 2, p. 215-220, 2006.
10.1111/j.1574-6968.2006.00267.x
0378-1097
http://repositorio.unifesp.br/handle/11600/28927
WOS:000237739500008
url http://dx.doi.org/10.1111/j.1574-6968.2006.00267.x
http://repositorio.unifesp.br/handle/11600/28927
identifier_str_mv Fems Microbiology Letters. Oxford: Blackwell Publishing, v. 259, n. 2, p. 215-220, 2006.
10.1111/j.1574-6968.2006.00267.x
0378-1097
WOS:000237739500008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fems Microbiology Letters
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 215-220
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268342027943936

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