Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/57971 http://dx.doi.org/10.7717/peerj.1704 |
Resumo: | Marine organisms are a prolific source of drug leads in a variety of therapeutic areas. In the last few years, biomedical, pharmaceutical and nutraceutical industries have shown growing interest in novel compounds from marine organisms, including macroalgae. Cystoseira is a genus of Phaeophyceae (Fucales) macroalgae known to contain bioactive compounds. Organic extracts (hexane, diethyl ether, ethyl acetate and methanol extracts) from three Cystoseira species (C. humilis, C. tamariscifolia and C. usneoides) were evaluated for their total phenolic content, radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'- azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals, and antiproliferative activity against a human hepatocarcinoma cell line (HepG2 cells). C. tamariscifolia had the highest TPC and RSA. The hexane extract of C. tamariscifolia (CTH) had the highest cytotoxic activity (IC50 = 2.31 mu g/mL), and was further tested in four human tumor (cervical adenocarcinoma HeLa; gastric adenocarcinoma AGS; colorectal adenocarcinoma HCT-15; neuroblastoma SH-SY5Y), and two non-tumor (murine bone marrow stroma S17 and human umbilical vein endothelial HUVEC) cell lines in order to determine its selectivity. CTH strongly reduced viability of all tumor cell lines, especially of HepG2 cells. Cytotoxicity was particularly selective for the latter cells with a selectivity index = 12.6 as compared to non-tumor cells. Incubation with CTH led to a 2-fold decrease of HepG2 cell proliferation as shown by the bromodeoxyuridine (BrdU) incorporation assay. CTH-treated HepG2 cells presented also pro-apoptotic features, such as increased Annexin Wpropidium iodide (PI) binding and dose-dependent morphological alterations in DAPI-stained cells. Moreover, it had a noticeable disaggregating effect on 3D multicellular tumor spheroids. Deme boxy cystoketal chromane, a derivative of the meroditerpenoid cystoketal, was identified as the active compound in CTH and was shown to display selective in vitro cYtotoxicitY towards HepG2 cells. |
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Vizetto-Duarte, CatarinaCustodio, LuisaAcosta, GerardoLago, Joao H. G.M[UNIFESP]Morais, Thiago R. [UNIFESP]de Sousa, Carolina BrunoGangadhar, Katkam N.Rodrigues, Maria JoaoPereira, HugoLima, Raquel T.Helena Vasconcelos, M.Barreiro, LuisaRauter, Amelia P.Albericioi, FernandoVarela, Joao2020-08-21T17:00:23Z2020-08-21T17:00:23Z2016Peerj. London, v. 4, p. -, 2016.2167-8359https://repositorio.unifesp.br/handle/11600/57971http://dx.doi.org/10.7717/peerj.1704WOS000370949200004.pdf10.7717/peerj.1704WOS:000370949200004Marine organisms are a prolific source of drug leads in a variety of therapeutic areas. In the last few years, biomedical, pharmaceutical and nutraceutical industries have shown growing interest in novel compounds from marine organisms, including macroalgae. Cystoseira is a genus of Phaeophyceae (Fucales) macroalgae known to contain bioactive compounds. Organic extracts (hexane, diethyl ether, ethyl acetate and methanol extracts) from three Cystoseira species (C. humilis, C. tamariscifolia and C. usneoides) were evaluated for their total phenolic content, radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'- azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals, and antiproliferative activity against a human hepatocarcinoma cell line (HepG2 cells). C. tamariscifolia had the highest TPC and RSA. The hexane extract of C. tamariscifolia (CTH) had the highest cytotoxic activity (IC50 = 2.31 mu g/mL), and was further tested in four human tumor (cervical adenocarcinoma HeLa; gastric adenocarcinoma AGS; colorectal adenocarcinoma HCT-15; neuroblastoma SH-SY5Y), and two non-tumor (murine bone marrow stroma S17 and human umbilical vein endothelial HUVEC) cell lines in order to determine its selectivity. CTH strongly reduced viability of all tumor cell lines, especially of HepG2 cells. Cytotoxicity was particularly selective for the latter cells with a selectivity index = 12.6 as compared to non-tumor cells. Incubation with CTH led to a 2-fold decrease of HepG2 cell proliferation as shown by the bromodeoxyuridine (BrdU) incorporation assay. CTH-treated HepG2 cells presented also pro-apoptotic features, such as increased Annexin Wpropidium iodide (PI) binding and dose-dependent morphological alterations in DAPI-stained cells. Moreover, it had a noticeable disaggregating effect on 3D multicellular tumor spheroids. Deme boxy cystoketal chromane, a derivative of the meroditerpenoid cystoketal, was identified as the active compound in CTH and was shown to display selective in vitro cYtotoxicitY towards HepG2 cells.SEABIOMEDXtremeBioFoundation for Science and Technology (FCT)Portuguese National BudgetFCTCAPESCNPqUniv Algarve, Fac Sci & Technol, Ctr Marine Sci, Campus Gambelas, Faro, PortugalInst Res Biomed Barcelona, Chem & Mol Pharmacol, Barcelona Sci Pk, Barcelona, SpainCIBER BNN, Networking Ctr Bioengn Biomat & Nanomed, Barcelona Sci Pk, Barcelona, SpainUniv Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Sao Paulo, BrazilUniv Nova Lisboa, Inst Tecnol Quim & Biol, P-1200 Lisbon, PortugalUniv Porto, Inst Invest & Inovacao Saude, Rua Alfredo Allen, Oporto, PortugalUniv Porto, Canc Drug Resistance Grp, IPATIMUP Inst Mol Pathol & Immunol, Rua Julio Amaral Carvalho, Oporto, PortugalUniv Porto, Dept Pathol & Oncol, Fac Med, Alameda Prof Hernani Monteiro, Oporto, PortugalUniv Porto, Fac Pharm, Dept Biol Sci, Rua Jorge Viterbo Ferreira, Oporto, PortugalUniv Lisbon, Fac Sci, Dept Chem & Biochem, Ctr Chem & Biochem, P-1699 Lisbon, PortugalUniv Barcelona, Dept Organ Chem, Barcelona, SpainUniv Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Sao Paulo, BrazilSEABIOMED: PTDC/MAR/103957/2008XtremeBio: PTDC/MAR-EST/4346/2012FCT: CCMAR/Multi/04326/2013FCT :IF/00049/2012Web of Science-engPeerj IncPeerjMarine natural productsBrown algaeAntioxidantAnti-hepatocarcinomaCystoseiraCan macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compoundsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondon4info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000370949200004.pdfapplication/pdf5096096${dspace.ui.url}/bitstream/11600/57971/1/WOS000370949200004.pdf5b27dade9c20045a769c3eab33ef96b3MD51open accessTEXTWOS000370949200004.pdf.txtWOS000370949200004.pdf.txtExtracted texttext/plain62936${dspace.ui.url}/bitstream/11600/57971/8/WOS000370949200004.pdf.txt7461e5868e71e186d88015e3d91b6c16MD58open accessTHUMBNAILWOS000370949200004.pdf.jpgWOS000370949200004.pdf.jpgIM Thumbnailimage/jpeg7381${dspace.ui.url}/bitstream/11600/57971/10/WOS000370949200004.pdf.jpgbb19c87ec4bb9a1fc08e6033ab43b015MD510open access11600/579712023-06-05 19:31:41.928open accessoai:repositorio.unifesp.br:11600/57971Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:31:41Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds |
title |
Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds |
spellingShingle |
Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds Vizetto-Duarte, Catarina Marine natural products Brown algae Antioxidant Anti-hepatocarcinoma Cystoseira |
title_short |
Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds |
title_full |
Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds |
title_fullStr |
Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds |
title_full_unstemmed |
Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds |
title_sort |
Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds |
author |
Vizetto-Duarte, Catarina |
author_facet |
Vizetto-Duarte, Catarina Custodio, Luisa Acosta, Gerardo Lago, Joao H. G.M[UNIFESP] Morais, Thiago R. [UNIFESP] de Sousa, Carolina Bruno Gangadhar, Katkam N. Rodrigues, Maria Joao Pereira, Hugo Lima, Raquel T. Helena Vasconcelos, M. Barreiro, Luisa Rauter, Amelia P. Albericioi, Fernando Varela, Joao |
author_role |
author |
author2 |
Custodio, Luisa Acosta, Gerardo Lago, Joao H. G.M[UNIFESP] Morais, Thiago R. [UNIFESP] de Sousa, Carolina Bruno Gangadhar, Katkam N. Rodrigues, Maria Joao Pereira, Hugo Lima, Raquel T. Helena Vasconcelos, M. Barreiro, Luisa Rauter, Amelia P. Albericioi, Fernando Varela, Joao |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vizetto-Duarte, Catarina Custodio, Luisa Acosta, Gerardo Lago, Joao H. G.M[UNIFESP] Morais, Thiago R. [UNIFESP] de Sousa, Carolina Bruno Gangadhar, Katkam N. Rodrigues, Maria Joao Pereira, Hugo Lima, Raquel T. Helena Vasconcelos, M. Barreiro, Luisa Rauter, Amelia P. Albericioi, Fernando Varela, Joao |
dc.subject.eng.fl_str_mv |
Marine natural products Brown algae Antioxidant Anti-hepatocarcinoma Cystoseira |
topic |
Marine natural products Brown algae Antioxidant Anti-hepatocarcinoma Cystoseira |
description |
Marine organisms are a prolific source of drug leads in a variety of therapeutic areas. In the last few years, biomedical, pharmaceutical and nutraceutical industries have shown growing interest in novel compounds from marine organisms, including macroalgae. Cystoseira is a genus of Phaeophyceae (Fucales) macroalgae known to contain bioactive compounds. Organic extracts (hexane, diethyl ether, ethyl acetate and methanol extracts) from three Cystoseira species (C. humilis, C. tamariscifolia and C. usneoides) were evaluated for their total phenolic content, radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'- azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals, and antiproliferative activity against a human hepatocarcinoma cell line (HepG2 cells). C. tamariscifolia had the highest TPC and RSA. The hexane extract of C. tamariscifolia (CTH) had the highest cytotoxic activity (IC50 = 2.31 mu g/mL), and was further tested in four human tumor (cervical adenocarcinoma HeLa; gastric adenocarcinoma AGS; colorectal adenocarcinoma HCT-15; neuroblastoma SH-SY5Y), and two non-tumor (murine bone marrow stroma S17 and human umbilical vein endothelial HUVEC) cell lines in order to determine its selectivity. CTH strongly reduced viability of all tumor cell lines, especially of HepG2 cells. Cytotoxicity was particularly selective for the latter cells with a selectivity index = 12.6 as compared to non-tumor cells. Incubation with CTH led to a 2-fold decrease of HepG2 cell proliferation as shown by the bromodeoxyuridine (BrdU) incorporation assay. CTH-treated HepG2 cells presented also pro-apoptotic features, such as increased Annexin Wpropidium iodide (PI) binding and dose-dependent morphological alterations in DAPI-stained cells. Moreover, it had a noticeable disaggregating effect on 3D multicellular tumor spheroids. Deme boxy cystoketal chromane, a derivative of the meroditerpenoid cystoketal, was identified as the active compound in CTH and was shown to display selective in vitro cYtotoxicitY towards HepG2 cells. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2020-08-21T17:00:23Z |
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2020-08-21T17:00:23Z |
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publishedVersion |
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Peerj. London, v. 4, p. -, 2016. |
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https://repositorio.unifesp.br/handle/11600/57971 http://dx.doi.org/10.7717/peerj.1704 |
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2167-8359 |
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WOS000370949200004.pdf |
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10.7717/peerj.1704 |
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Peerj. London, v. 4, p. -, 2016. 2167-8359 WOS000370949200004.pdf 10.7717/peerj.1704 WOS:000370949200004 |
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