ACE2-angiotensin-(1-7)-Mas axis in renal ischaemia/reperfusion injury in rats

• Autor(a) principal: Silveira, Katia D. da
• Data de Publicação: 2010
• Outros Autores: Bosco, Kenia S. Pompermayer, Diniz, Lucio R. L., Carmona, Adriana Karaoglanovic [UNIFESP], Cassali, Giovanni D., Bruna-Romero, Oscar, Sousa, Lirlandia P. de, Teixeira, Mauro M., Santos, Robson A. S., Simoes e Silva, Ana C., Ribeiro Vieira, Maria A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1042/CS20090554; http://repositorio.unifesp.br/handle/11600/33007
• topic: acute kidney injury; angiotensin I-converting enzyme (ACE); angiotensin I-converting enzyme 2 (ACE2); angiotensin-(1-7) [Ang-(1-7)]; angiotensin II (AngII); Mas receptor

AngII (angiotensin II), ACE (angiotensin I-converting enzyme) and the AT(1) receptor (AngII type I receptor) are associated with the inflammatory process and microvascular dysfunction of AKI (acute kidney injury) induced by renal I/R (ischaemia/reperfusion). However, Ang-(1-7) [angiotensin-(1-7)], ACE2 (angiotensin I-converting enzyme 2) and the Mas receptor also play a role in renal disease models. Therefore, in the present study, we have examined the renal profile of Ang-(1-7), ACE2 and the Mas receptor in renal I/R and compared them with that of AngII, ACE and the AT(1) receptor. Male Wistar rats were submitted to left nephrectomy and ischaemia (45 min) followed by reperfusion (2 or 4 h) in the right kidney. At 4 h of reperfusion, renal AngII was increased (P < 0.01) and renal Ang-(1-7) was decreased substantially (P < 0.05), although plasma levels of both angiotensins were unchanged. in addition, renal I/R decreased the renal mRNA expression of renin (P < 0.05), AT(1) receptors (P < 0.001) and ACE2 (P < 0.05). At 2 and 4 h of reperfusion, renal ACE activity was reduced (P < 0.05). On the other hand, renal expression of the Mas receptor was greatly increased at 4 h of reperfusion (P < 0.01), which was confirmed by immunohistochemical and Western blot analysis. in conclusion, increased renal expression of the Mas receptor associated with changes in the RAS (renin-angiotensin-system)-related peptidases support an important role for the ACE2 Ang-(1-7) Mas axis in AKI.