Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Lovato, Diogo Ventura [UNIFESP]
Data de Publicação: 2011
Outros Autores: Amino, Rogerio [UNIFESP], Gonzalez, Yamile, Miranda, Antonio [UNIFESP], Schenkman, Sergio [UNIFESP], Tanaka, Aparecida Sadae [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
dARK ID: ark:/48912/001300000d42n
Texto Completo: http://dx.doi.org/10.1016/j.exppara.2011.09.002
http://repositorio.unifesp.br/handle/11600/34242
Resumo: Infestins are Kazal-type serine protease inhibitors described in the midgut of Triatoma infestans, Chagas disease vector. of all infestins, only infestin 1R (INF1R) does not control host blood coagulation, due to its inhibitory specificity for chymotrypsin-like proteases. We further investigated the effect of INF1R on cell infection by Trypanosoma cruzi. the importance of INF1R reactive site to inhibit T. cruzi cell invasion was confirmed using 1RSFTI, a synthetic cyclic peptide containing the inhibitor reactive site region hybridized to the Sunflower Trypsin Inhibitor-1 (SFTI-1). Our results suggest that INF1R efficiently inhibited parasite cell invasion. for the first time, a serine protease inhibitor, derived from T. infestans, was shown to impair cell invasion by T. cruzi, representing possible new target in parasite cell invasion. (C) 2011 Elsevier Inc. All rights reserved.
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spelling Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruziProtease inhibitorSubtilisin ATriatoma infestansTrypanosoma cruziInfestins are Kazal-type serine protease inhibitors described in the midgut of Triatoma infestans, Chagas disease vector. of all infestins, only infestin 1R (INF1R) does not control host blood coagulation, due to its inhibitory specificity for chymotrypsin-like proteases. We further investigated the effect of INF1R on cell infection by Trypanosoma cruzi. the importance of INF1R reactive site to inhibit T. cruzi cell invasion was confirmed using 1RSFTI, a synthetic cyclic peptide containing the inhibitor reactive site region hybridized to the Sunflower Trypsin Inhibitor-1 (SFTI-1). Our results suggest that INF1R efficiently inhibited parasite cell invasion. for the first time, a serine protease inhibitor, derived from T. infestans, was shown to impair cell invasion by T. cruzi, representing possible new target in parasite cell invasion. (C) 2011 Elsevier Inc. All rights reserved.Universidade Federal de São Paulo, Dept Bioquim, Escola Paulista Med, São Paulo, BrazilUniv La Habana, Fac Biol, Ctr Estudio Prot, Havana, CubaUniversidade Federal de São Paulo, Dept Biofis, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Bioquim, Escola Paulista Med, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biofis, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP: 05/03514-9FAPESP: 02/12593-1FAPESP: 09/50434-1CNPq: 470070/2004-8Elsevier B.V.Universidade Federal de São Paulo (UNIFESP)Univ La HabanaLovato, Diogo Ventura [UNIFESP]Amino, Rogerio [UNIFESP]Gonzalez, YamileMiranda, Antonio [UNIFESP]Schenkman, Sergio [UNIFESP]Tanaka, Aparecida Sadae [UNIFESP]2016-01-24T14:17:28Z2016-01-24T14:17:28Z2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion362-367application/pdfhttp://dx.doi.org/10.1016/j.exppara.2011.09.002Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 129, n. 4, p. 362-367, 2011.10.1016/j.exppara.2011.09.002WOS000297777300006.pdf0014-4894http://repositorio.unifesp.br/handle/11600/34242WOS:000297777300006ark:/48912/001300000d42nengExperimental Parasitologyinfo:eu-repo/semantics/openAccesshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policyreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-08T09:33:50Zoai:repositorio.unifesp.br/:11600/34242Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-12-11T20:12:32.535672Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi
title Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi
spellingShingle Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi
Lovato, Diogo Ventura [UNIFESP]
Protease inhibitor
Subtilisin A
Triatoma infestans
Trypanosoma cruzi
title_short Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi
title_full Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi
title_fullStr Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi
title_full_unstemmed Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi
title_sort Infestin 1R, an intestinal subtilisin inhibitor from Triatoma infestans able to impair mammalian cell invasion by Trypanosoma cruzi
author Lovato, Diogo Ventura [UNIFESP]
author_facet Lovato, Diogo Ventura [UNIFESP]
Amino, Rogerio [UNIFESP]
Gonzalez, Yamile
Miranda, Antonio [UNIFESP]
Schenkman, Sergio [UNIFESP]
Tanaka, Aparecida Sadae [UNIFESP]
author_role author
author2 Amino, Rogerio [UNIFESP]
Gonzalez, Yamile
Miranda, Antonio [UNIFESP]
Schenkman, Sergio [UNIFESP]
Tanaka, Aparecida Sadae [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Univ La Habana
dc.contributor.author.fl_str_mv Lovato, Diogo Ventura [UNIFESP]
Amino, Rogerio [UNIFESP]
Gonzalez, Yamile
Miranda, Antonio [UNIFESP]
Schenkman, Sergio [UNIFESP]
Tanaka, Aparecida Sadae [UNIFESP]
dc.subject.por.fl_str_mv Protease inhibitor
Subtilisin A
Triatoma infestans
Trypanosoma cruzi
topic Protease inhibitor
Subtilisin A
Triatoma infestans
Trypanosoma cruzi
description Infestins are Kazal-type serine protease inhibitors described in the midgut of Triatoma infestans, Chagas disease vector. of all infestins, only infestin 1R (INF1R) does not control host blood coagulation, due to its inhibitory specificity for chymotrypsin-like proteases. We further investigated the effect of INF1R on cell infection by Trypanosoma cruzi. the importance of INF1R reactive site to inhibit T. cruzi cell invasion was confirmed using 1RSFTI, a synthetic cyclic peptide containing the inhibitor reactive site region hybridized to the Sunflower Trypsin Inhibitor-1 (SFTI-1). Our results suggest that INF1R efficiently inhibited parasite cell invasion. for the first time, a serine protease inhibitor, derived from T. infestans, was shown to impair cell invasion by T. cruzi, representing possible new target in parasite cell invasion. (C) 2011 Elsevier Inc. All rights reserved.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
2016-01-24T14:17:28Z
2016-01-24T14:17:28Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.exppara.2011.09.002
Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 129, n. 4, p. 362-367, 2011.
10.1016/j.exppara.2011.09.002
WOS000297777300006.pdf
0014-4894
http://repositorio.unifesp.br/handle/11600/34242
WOS:000297777300006
dc.identifier.dark.fl_str_mv ark:/48912/001300000d42n
url http://dx.doi.org/10.1016/j.exppara.2011.09.002
http://repositorio.unifesp.br/handle/11600/34242
identifier_str_mv Experimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 129, n. 4, p. 362-367, 2011.
10.1016/j.exppara.2011.09.002
WOS000297777300006.pdf
0014-4894
WOS:000297777300006
ark:/48912/001300000d42n
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Experimental Parasitology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
eu_rights_str_mv openAccess
rights_invalid_str_mv http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.format.none.fl_str_mv 362-367
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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