Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/7890 http://dx.doi.org/10.1590/S0102-86502013000800002 |
Resumo: | PURPOSE: To investigate if the ethyl-pyruvate solution could reduce mortality in AP and/or diminish the acute lung injury. METHODS: Forty male rats, weighing between 270 to 330 grams were operated. An experimental model of severe AP by injection of 0.1ml/100g of 2.5% sodium taurocholate into the bilio-pancreatic duct was utilized. The rats were divided into two groups of ten animals each: CT - control (treatment with 50ml/kg of Ringer's solution, intraperitoneal) and EP (treatment with 50ml/kg of Ringer ethyl- pyruvate solution, intra-peritoneal), three hours following AP induction. After six hours, a new infusion of the treatment solution was performed in each group. Two hours later, the animals were killed and the pulmonary parenchyma was resected for biomolecular analysis, consisting of: interleukin, myeloperoxidase, MDA, nitric oxide, metalloproteinases and heat shock protein. In the second part of the experiment, another, 20 rats were randomly divided into EP and CT groups, in order to evaluate a survival comparison between the two groups. RESULTS: There were no significant differences in IL-1B,IL-10, MMP-9, HSP70, nitric oxide, MPO, MDA (lipidic peroxidation) concerning both groups. The levels of IL-6 were significantly diminished in the EP group. Furthermore, the MMP-2 levels were also reduced in the EP group (p<0.05). The animals from the EP treatment groups had improved survival, when compared to control group (p<0.05). CONCLUSION: The ethyl-pyruvate diminishes acute lung injury inflammatory response in acute pancreatitis and ameliorates survival when compared to control group, in the experimental model of necrotizing acute pancreatitis. |
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Matone, Jacques [UNIFESP]Moretti, Ana Iochabel SoaresApodaca-Torrez, Franz Robert [UNIFESP]Goldenberg, Alberto [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)2015-06-14T13:45:34Z2015-06-14T13:45:34Z2013-08-01Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 8, p. 559-567, 2013.0102-8650http://repositorio.unifesp.br/handle/11600/7890http://dx.doi.org/10.1590/S0102-86502013000800002S0102-86502013000800002.pdfS0102-8650201300080000210.1590/S0102-86502013000800002PURPOSE: To investigate if the ethyl-pyruvate solution could reduce mortality in AP and/or diminish the acute lung injury. METHODS: Forty male rats, weighing between 270 to 330 grams were operated. An experimental model of severe AP by injection of 0.1ml/100g of 2.5% sodium taurocholate into the bilio-pancreatic duct was utilized. The rats were divided into two groups of ten animals each: CT - control (treatment with 50ml/kg of Ringer's solution, intraperitoneal) and EP (treatment with 50ml/kg of Ringer ethyl- pyruvate solution, intra-peritoneal), three hours following AP induction. After six hours, a new infusion of the treatment solution was performed in each group. Two hours later, the animals were killed and the pulmonary parenchyma was resected for biomolecular analysis, consisting of: interleukin, myeloperoxidase, MDA, nitric oxide, metalloproteinases and heat shock protein. In the second part of the experiment, another, 20 rats were randomly divided into EP and CT groups, in order to evaluate a survival comparison between the two groups. RESULTS: There were no significant differences in IL-1B,IL-10, MMP-9, HSP70, nitric oxide, MPO, MDA (lipidic peroxidation) concerning both groups. The levels of IL-6 were significantly diminished in the EP group. Furthermore, the MMP-2 levels were also reduced in the EP group (p<0.05). The animals from the EP treatment groups had improved survival, when compared to control group (p<0.05). CONCLUSION: The ethyl-pyruvate diminishes acute lung injury inflammatory response in acute pancreatitis and ameliorates survival when compared to control group, in the experimental model of necrotizing acute pancreatitis.UNIFESP-EPM Postgraduate Program in Gastrointestinal SurgeryUSP Department of Emergency MedicineUNIFESP-EPM Department of SurgeryUNIFESP, EPM, Postgraduate Program in Gastrointestinal SurgeryUNIFESP, EPM Department of SurgerySciELO559-567engSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirurgica BrasileiraPancreatitisAcute NecrotizingPyruvic AcidInflammationRatsEthyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS0102-86502013000800002.pdfapplication/pdf2295054${dspace.ui.url}/bitstream/11600/7890/1/S0102-86502013000800002.pdf4316eb8e312c8420345ff57afc3f2659MD51open accessTEXTS0102-86502013000800002.pdf.txtS0102-86502013000800002.pdf.txtExtracted texttext/plain33949${dspace.ui.url}/bitstream/11600/7890/2/S0102-86502013000800002.pdf.txt19a4bf5995eda14eafcbe63c9184e1daMD52open access11600/78902021-09-30 11:01:51.411open accessoai:repositorio.unifesp.br:11600/7890Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-09-30T14:01:51Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis |
title |
Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis |
spellingShingle |
Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis Matone, Jacques [UNIFESP] Pancreatitis Acute Necrotizing Pyruvic Acid Inflammation Rats |
title_short |
Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis |
title_full |
Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis |
title_fullStr |
Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis |
title_full_unstemmed |
Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis |
title_sort |
Ethyl-pyruvate reduces lung injury matrix metalloproteinases and cytokines and improves survival in experimental model of severe acute pancreatitis |
author |
Matone, Jacques [UNIFESP] |
author_facet |
Matone, Jacques [UNIFESP] Moretti, Ana Iochabel Soares Apodaca-Torrez, Franz Robert [UNIFESP] Goldenberg, Alberto [UNIFESP] |
author_role |
author |
author2 |
Moretti, Ana Iochabel Soares Apodaca-Torrez, Franz Robert [UNIFESP] Goldenberg, Alberto [UNIFESP] |
author2_role |
author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Matone, Jacques [UNIFESP] Moretti, Ana Iochabel Soares Apodaca-Torrez, Franz Robert [UNIFESP] Goldenberg, Alberto [UNIFESP] |
dc.subject.eng.fl_str_mv |
Pancreatitis Acute Necrotizing Pyruvic Acid Inflammation Rats |
topic |
Pancreatitis Acute Necrotizing Pyruvic Acid Inflammation Rats |
description |
PURPOSE: To investigate if the ethyl-pyruvate solution could reduce mortality in AP and/or diminish the acute lung injury. METHODS: Forty male rats, weighing between 270 to 330 grams were operated. An experimental model of severe AP by injection of 0.1ml/100g of 2.5% sodium taurocholate into the bilio-pancreatic duct was utilized. The rats were divided into two groups of ten animals each: CT - control (treatment with 50ml/kg of Ringer's solution, intraperitoneal) and EP (treatment with 50ml/kg of Ringer ethyl- pyruvate solution, intra-peritoneal), three hours following AP induction. After six hours, a new infusion of the treatment solution was performed in each group. Two hours later, the animals were killed and the pulmonary parenchyma was resected for biomolecular analysis, consisting of: interleukin, myeloperoxidase, MDA, nitric oxide, metalloproteinases and heat shock protein. In the second part of the experiment, another, 20 rats were randomly divided into EP and CT groups, in order to evaluate a survival comparison between the two groups. RESULTS: There were no significant differences in IL-1B,IL-10, MMP-9, HSP70, nitric oxide, MPO, MDA (lipidic peroxidation) concerning both groups. The levels of IL-6 were significantly diminished in the EP group. Furthermore, the MMP-2 levels were also reduced in the EP group (p<0.05). The animals from the EP treatment groups had improved survival, when compared to control group (p<0.05). CONCLUSION: The ethyl-pyruvate diminishes acute lung injury inflammatory response in acute pancreatitis and ameliorates survival when compared to control group, in the experimental model of necrotizing acute pancreatitis. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013-08-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:45:34Z |
dc.date.available.fl_str_mv |
2015-06-14T13:45:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 8, p. 559-567, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/7890 http://dx.doi.org/10.1590/S0102-86502013000800002 |
dc.identifier.issn.none.fl_str_mv |
0102-8650 |
dc.identifier.file.none.fl_str_mv |
S0102-86502013000800002.pdf |
dc.identifier.scielo.none.fl_str_mv |
S0102-86502013000800002 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0102-86502013000800002 |
identifier_str_mv |
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 8, p. 559-567, 2013. 0102-8650 S0102-86502013000800002.pdf S0102-86502013000800002 10.1590/S0102-86502013000800002 |
url |
http://repositorio.unifesp.br/handle/11600/7890 http://dx.doi.org/10.1590/S0102-86502013000800002 |
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eng |
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Acta Cirurgica Brasileira |
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559-567 |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
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