Virulence properties of atypical EPEC strains

Detalhes bibliográficos
Autor(a) principal: Pelayo, J. S.
Data de Publicação: 1999
Outros Autores: Scaletsky, Isabel Cristina Affonso [UNIFESP], Pedroso, M. Z., Sperandio, V, Giron, J. A., Frankel, G., Trabulsi, Luiz Rachid [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1099/00222615-48-1-41
http://repositorio.unifesp.br/handle/11600/44500
Resumo: Virulence properties of 31 atypical enteropathogenic Escherichia coli (EPEC) strains isolated from cases of diarrhoea were examined. All except two strains adhered to HEp-2 cells in a localised adherence-like (LAL) pattern. With the exception of two strains, all were fluorescent actin staining (FAS) positive. Gentamicin HEp-2 invasion assay studies showed that all strains were invasive, Transmission electron microscopy of infected HEp-2 cells showed the characteristic attaching and effacing lesion and invasion of the cultured cells. Of the nine strains that hybridised with a DNA probe for a-haemolysin, five were haemolytic within 3 h of incubation, while the remaining strains were haemolytic only after incubation for 24 h, Three strains produced enterohaemolysin on blood agar, None of the 31 strains of E. coli induced fluid accumulation in the rabbit intestinal loop assay or displayed cytotoxic effects in HeLa and Vero cells. All the strains belonging to serotypes O26:H11, O26:H- and O119:H2 expressed intimin beta, whereas all the strains from serotype O55:H7 expressed intimin gamma, The strains belonging to serogroup O111 expressed a non-typable intimin, The participation of intimin in LAL was supported by adhesion inhibition experiments in which antibodies to intimin significantly reduced the level of LAL.
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spelling Virulence properties of atypical EPEC strainsVirulence properties of 31 atypical enteropathogenic Escherichia coli (EPEC) strains isolated from cases of diarrhoea were examined. All except two strains adhered to HEp-2 cells in a localised adherence-like (LAL) pattern. With the exception of two strains, all were fluorescent actin staining (FAS) positive. Gentamicin HEp-2 invasion assay studies showed that all strains were invasive, Transmission electron microscopy of infected HEp-2 cells showed the characteristic attaching and effacing lesion and invasion of the cultured cells. Of the nine strains that hybridised with a DNA probe for a-haemolysin, five were haemolytic within 3 h of incubation, while the remaining strains were haemolytic only after incubation for 24 h, Three strains produced enterohaemolysin on blood agar, None of the 31 strains of E. coli induced fluid accumulation in the rabbit intestinal loop assay or displayed cytotoxic effects in HeLa and Vero cells. All the strains belonging to serotypes O26:H11, O26:H- and O119:H2 expressed intimin beta, whereas all the strains from serotype O55:H7 expressed intimin gamma, The strains belonging to serogroup O111 expressed a non-typable intimin, The participation of intimin in LAL was supported by adhesion inhibition experiments in which antibodies to intimin significantly reduced the level of LAL.Univ Sao Paulo, Dept Microbiol, BR-05508900 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, BR-04023062 Sao Paulo, BrazilBenemerita Univ Autonoma Puebla, Ctr Invest Ciencias Microbiol, Puebla 72570, MexicoUniv London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AZ, EnglandUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, BR-04023062 Sao Paulo, BrazilWeb of ScienceLippincott Williams & WilkinsUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Benemerita Univ Autonoma PueblaUniv London Imperial Coll Sci Technol & MedPelayo, J. S.Scaletsky, Isabel Cristina Affonso [UNIFESP]Pedroso, M. Z.Sperandio, VGiron, J. A.Frankel, G.Trabulsi, Luiz Rachid [UNIFESP]2018-06-15T18:07:26Z2018-06-15T18:07:26Z1999-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion41-49http://dx.doi.org/10.1099/00222615-48-1-41Journal Of Medical Microbiology. Philadelphia: Lippincott Williams & Wilkins, v. 48, n. 1, p. 41-49, 1999.10.1099/00222615-48-1-410022-2615http://repositorio.unifesp.br/handle/11600/44500WOS:000079235200007engJournal Of Medical Microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T13:57:07Zoai:repositorio.unifesp.br/:11600/44500Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T13:57:07Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Virulence properties of atypical EPEC strains
title Virulence properties of atypical EPEC strains
spellingShingle Virulence properties of atypical EPEC strains
Pelayo, J. S.
title_short Virulence properties of atypical EPEC strains
title_full Virulence properties of atypical EPEC strains
title_fullStr Virulence properties of atypical EPEC strains
title_full_unstemmed Virulence properties of atypical EPEC strains
title_sort Virulence properties of atypical EPEC strains
author Pelayo, J. S.
author_facet Pelayo, J. S.
Scaletsky, Isabel Cristina Affonso [UNIFESP]
Pedroso, M. Z.
Sperandio, V
Giron, J. A.
Frankel, G.
Trabulsi, Luiz Rachid [UNIFESP]
author_role author
author2 Scaletsky, Isabel Cristina Affonso [UNIFESP]
Pedroso, M. Z.
Sperandio, V
Giron, J. A.
Frankel, G.
Trabulsi, Luiz Rachid [UNIFESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Benemerita Univ Autonoma Puebla
Univ London Imperial Coll Sci Technol & Med
dc.contributor.author.fl_str_mv Pelayo, J. S.
Scaletsky, Isabel Cristina Affonso [UNIFESP]
Pedroso, M. Z.
Sperandio, V
Giron, J. A.
Frankel, G.
Trabulsi, Luiz Rachid [UNIFESP]
description Virulence properties of 31 atypical enteropathogenic Escherichia coli (EPEC) strains isolated from cases of diarrhoea were examined. All except two strains adhered to HEp-2 cells in a localised adherence-like (LAL) pattern. With the exception of two strains, all were fluorescent actin staining (FAS) positive. Gentamicin HEp-2 invasion assay studies showed that all strains were invasive, Transmission electron microscopy of infected HEp-2 cells showed the characteristic attaching and effacing lesion and invasion of the cultured cells. Of the nine strains that hybridised with a DNA probe for a-haemolysin, five were haemolytic within 3 h of incubation, while the remaining strains were haemolytic only after incubation for 24 h, Three strains produced enterohaemolysin on blood agar, None of the 31 strains of E. coli induced fluid accumulation in the rabbit intestinal loop assay or displayed cytotoxic effects in HeLa and Vero cells. All the strains belonging to serotypes O26:H11, O26:H- and O119:H2 expressed intimin beta, whereas all the strains from serotype O55:H7 expressed intimin gamma, The strains belonging to serogroup O111 expressed a non-typable intimin, The participation of intimin in LAL was supported by adhesion inhibition experiments in which antibodies to intimin significantly reduced the level of LAL.
publishDate 1999
dc.date.none.fl_str_mv 1999-01-01
2018-06-15T18:07:26Z
2018-06-15T18:07:26Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1099/00222615-48-1-41
Journal Of Medical Microbiology. Philadelphia: Lippincott Williams & Wilkins, v. 48, n. 1, p. 41-49, 1999.
10.1099/00222615-48-1-41
0022-2615
http://repositorio.unifesp.br/handle/11600/44500
WOS:000079235200007
url http://dx.doi.org/10.1099/00222615-48-1-41
http://repositorio.unifesp.br/handle/11600/44500
identifier_str_mv Journal Of Medical Microbiology. Philadelphia: Lippincott Williams & Wilkins, v. 48, n. 1, p. 41-49, 1999.
10.1099/00222615-48-1-41
0022-2615
WOS:000079235200007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Medical Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 41-49
dc.publisher.none.fl_str_mv Lippincott Williams & Wilkins
publisher.none.fl_str_mv Lippincott Williams & Wilkins
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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