Virulence properties of atypical EPEC strains
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1099/00222615-48-1-41 http://repositorio.unifesp.br/handle/11600/44500 |
Resumo: | Virulence properties of 31 atypical enteropathogenic Escherichia coli (EPEC) strains isolated from cases of diarrhoea were examined. All except two strains adhered to HEp-2 cells in a localised adherence-like (LAL) pattern. With the exception of two strains, all were fluorescent actin staining (FAS) positive. Gentamicin HEp-2 invasion assay studies showed that all strains were invasive, Transmission electron microscopy of infected HEp-2 cells showed the characteristic attaching and effacing lesion and invasion of the cultured cells. Of the nine strains that hybridised with a DNA probe for a-haemolysin, five were haemolytic within 3 h of incubation, while the remaining strains were haemolytic only after incubation for 24 h, Three strains produced enterohaemolysin on blood agar, None of the 31 strains of E. coli induced fluid accumulation in the rabbit intestinal loop assay or displayed cytotoxic effects in HeLa and Vero cells. All the strains belonging to serotypes O26:H11, O26:H- and O119:H2 expressed intimin beta, whereas all the strains from serotype O55:H7 expressed intimin gamma, The strains belonging to serogroup O111 expressed a non-typable intimin, The participation of intimin in LAL was supported by adhesion inhibition experiments in which antibodies to intimin significantly reduced the level of LAL. |
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Virulence properties of atypical EPEC strainsVirulence properties of 31 atypical enteropathogenic Escherichia coli (EPEC) strains isolated from cases of diarrhoea were examined. All except two strains adhered to HEp-2 cells in a localised adherence-like (LAL) pattern. With the exception of two strains, all were fluorescent actin staining (FAS) positive. Gentamicin HEp-2 invasion assay studies showed that all strains were invasive, Transmission electron microscopy of infected HEp-2 cells showed the characteristic attaching and effacing lesion and invasion of the cultured cells. Of the nine strains that hybridised with a DNA probe for a-haemolysin, five were haemolytic within 3 h of incubation, while the remaining strains were haemolytic only after incubation for 24 h, Three strains produced enterohaemolysin on blood agar, None of the 31 strains of E. coli induced fluid accumulation in the rabbit intestinal loop assay or displayed cytotoxic effects in HeLa and Vero cells. All the strains belonging to serotypes O26:H11, O26:H- and O119:H2 expressed intimin beta, whereas all the strains from serotype O55:H7 expressed intimin gamma, The strains belonging to serogroup O111 expressed a non-typable intimin, The participation of intimin in LAL was supported by adhesion inhibition experiments in which antibodies to intimin significantly reduced the level of LAL.Univ Sao Paulo, Dept Microbiol, BR-05508900 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, BR-04023062 Sao Paulo, BrazilBenemerita Univ Autonoma Puebla, Ctr Invest Ciencias Microbiol, Puebla 72570, MexicoUniv London Imperial Coll Sci Technol & Med, Dept Biochem, London SW7 2AZ, EnglandUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, BR-04023062 Sao Paulo, BrazilWeb of ScienceLippincott Williams & WilkinsUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Benemerita Univ Autonoma PueblaUniv London Imperial Coll Sci Technol & MedPelayo, J. S.Scaletsky, Isabel Cristina Affonso [UNIFESP]Pedroso, M. Z.Sperandio, VGiron, J. A.Frankel, G.Trabulsi, Luiz Rachid [UNIFESP]2018-06-15T18:07:26Z2018-06-15T18:07:26Z1999-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion41-49http://dx.doi.org/10.1099/00222615-48-1-41Journal Of Medical Microbiology. Philadelphia: Lippincott Williams & Wilkins, v. 48, n. 1, p. 41-49, 1999.10.1099/00222615-48-1-410022-2615http://repositorio.unifesp.br/handle/11600/44500WOS:000079235200007engJournal Of Medical Microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T13:57:07Zoai:repositorio.unifesp.br/:11600/44500Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T13:57:07Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Virulence properties of atypical EPEC strains |
title |
Virulence properties of atypical EPEC strains |
spellingShingle |
Virulence properties of atypical EPEC strains Pelayo, J. S. |
title_short |
Virulence properties of atypical EPEC strains |
title_full |
Virulence properties of atypical EPEC strains |
title_fullStr |
Virulence properties of atypical EPEC strains |
title_full_unstemmed |
Virulence properties of atypical EPEC strains |
title_sort |
Virulence properties of atypical EPEC strains |
author |
Pelayo, J. S. |
author_facet |
Pelayo, J. S. Scaletsky, Isabel Cristina Affonso [UNIFESP] Pedroso, M. Z. Sperandio, V Giron, J. A. Frankel, G. Trabulsi, Luiz Rachid [UNIFESP] |
author_role |
author |
author2 |
Scaletsky, Isabel Cristina Affonso [UNIFESP] Pedroso, M. Z. Sperandio, V Giron, J. A. Frankel, G. Trabulsi, Luiz Rachid [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) Benemerita Univ Autonoma Puebla Univ London Imperial Coll Sci Technol & Med |
dc.contributor.author.fl_str_mv |
Pelayo, J. S. Scaletsky, Isabel Cristina Affonso [UNIFESP] Pedroso, M. Z. Sperandio, V Giron, J. A. Frankel, G. Trabulsi, Luiz Rachid [UNIFESP] |
description |
Virulence properties of 31 atypical enteropathogenic Escherichia coli (EPEC) strains isolated from cases of diarrhoea were examined. All except two strains adhered to HEp-2 cells in a localised adherence-like (LAL) pattern. With the exception of two strains, all were fluorescent actin staining (FAS) positive. Gentamicin HEp-2 invasion assay studies showed that all strains were invasive, Transmission electron microscopy of infected HEp-2 cells showed the characteristic attaching and effacing lesion and invasion of the cultured cells. Of the nine strains that hybridised with a DNA probe for a-haemolysin, five were haemolytic within 3 h of incubation, while the remaining strains were haemolytic only after incubation for 24 h, Three strains produced enterohaemolysin on blood agar, None of the 31 strains of E. coli induced fluid accumulation in the rabbit intestinal loop assay or displayed cytotoxic effects in HeLa and Vero cells. All the strains belonging to serotypes O26:H11, O26:H- and O119:H2 expressed intimin beta, whereas all the strains from serotype O55:H7 expressed intimin gamma, The strains belonging to serogroup O111 expressed a non-typable intimin, The participation of intimin in LAL was supported by adhesion inhibition experiments in which antibodies to intimin significantly reduced the level of LAL. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-01-01 2018-06-15T18:07:26Z 2018-06-15T18:07:26Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1099/00222615-48-1-41 Journal Of Medical Microbiology. Philadelphia: Lippincott Williams & Wilkins, v. 48, n. 1, p. 41-49, 1999. 10.1099/00222615-48-1-41 0022-2615 http://repositorio.unifesp.br/handle/11600/44500 WOS:000079235200007 |
url |
http://dx.doi.org/10.1099/00222615-48-1-41 http://repositorio.unifesp.br/handle/11600/44500 |
identifier_str_mv |
Journal Of Medical Microbiology. Philadelphia: Lippincott Williams & Wilkins, v. 48, n. 1, p. 41-49, 1999. 10.1099/00222615-48-1-41 0022-2615 WOS:000079235200007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Medical Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
41-49 |
dc.publisher.none.fl_str_mv |
Lippincott Williams & Wilkins |
publisher.none.fl_str_mv |
Lippincott Williams & Wilkins |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268396355715072 |