Cross-reactivity of anti-HIV-1 T cell immune responses among the major HIV-1 clades in HIV-1-positive individuals from 4 continents

• Autor(a) principal: Coplan, P. M.
• Data de Publicação: 2005
• Outros Autores: Gupta, S. B., Dubey, S. A., Pitisuttithum, P., Nikas, A., Mbewe, B., Vardas, E., Schechter, M., Kallas, Esper Georges [UNIFESP], Freed, D. C., Fu, T. M., Mast, C. T., Puthavathana, P., Kublin, J., Collins, K. B., Chisi, J., Pendame, R., Thaler, S. J., Gray, G., Mcintyre, J., Straus, W. L., Condra, J. H., Mehrotra, D. V., Guess, H. A., Emini, E. A., Shiver, J. W.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.1086/428450; http://repositorio.unifesp.br/handle/11600/28261

Background. the genetic diversity of human immunodeficiency virus type 1 (HIV-1) raises the question of whether vaccines that include a component to elicit antiviral T cell immunity based on a single viral genetic clade could provide cellular immune protection against divergent HIV-1 clades. Therefore, we quantified the cross-clade reactivity, among unvaccinated individuals, of anti-HIV-1 T cell responses to the infecting HIV-1 clade relative to other major circulating clades.Methods. Cellular immune responses to HIV-1 clades A, B, and C were compared by standardized interferon-gamma enzyme-linked immunospot assays among 250 unvaccinated individuals, infected with diverse HIV-1 clades, from Brazil, Malawi, South Africa, Thailand, and the United States. Cross-clade reactivity was evaluated by use of the ratio of responses to heterologous versus homologous ( infecting) clades of HIV-1.Results. Cellular immune responses were predominantly focused on viral Gag and Nef proteins. Cross-clade reactivity of cellular immune responses to HIV-1 clade A, B, and C proteins was substantial for Nef proteins ( ratio, 0.97 [95% confidence interval, 0.89-1.05]) and lower for Gag proteins ( ratio, 0.67 [ 95% confidence interval, 0.62-0.73]). the difference in cross-clade reactivity to Nef and Gag proteins was significant (P < .0001).Conclusions. Cross-clade reactivity of cellular immune responses can be substantial but varies by viral protein.