Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-879X2009000900009 http://repositorio.unifesp.br/handle/11600/5247 |
Resumo: | The generation of bradykinin (BK; Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg) in blood and kallidin (Lys-BK) in tissues by the action of the kallikrein-kinin system has received little attention in non-mammalian vertebrates. In mammals, kallidin can be generated by the coronary endothelium and myocytes in response to ischemia, mediating cardioprotective events. The plasma of birds lacks two key components of the kallikrein-kinin system: the low molecular weight kininogen and a prekallikrein activator analogous to mammalian factor XII, but treatment with bovine plasma kallikrein generates ornitho-kinin [Thr6,Leu8]-BK. The possible cardioprotective effect of ornitho-kinin infusion was investigated in an anesthetized, open-chest chicken model of acute coronary occlusion. A branch of the left main coronary artery was reversibly ligated to produce ischemia followed by reperfusion, after which the degree of myocardial necrosis (infarct size as a percent of area at risk) was assessed by tetrazolium staining. The iv injection of a low dose of ornitho-kinin (4 µg/kg) reduced mean arterial pressure from 88 ± 12 to 42 ± 7 mmHg and increased heart rate from 335 ± 38 to 402 ± 45 bpm (N = 5). The size of the infarct was reduced by pretreatment with ornitho-kinin (500 µg/kg infused over a period of 5 min) from 35 ± 3 to 10 ± 2% of the area at risk. These results suggest that the physiological role of the kallikrein-kinin system is preserved in this animal model in spite of the absence of two key components, i.e., low molecular weight kininogen and factor XII. |
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Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusionChickenInfarct sizeKallikrein-kinin systemOrnitho-kininFactor XIIThe generation of bradykinin (BK; Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg) in blood and kallidin (Lys-BK) in tissues by the action of the kallikrein-kinin system has received little attention in non-mammalian vertebrates. In mammals, kallidin can be generated by the coronary endothelium and myocytes in response to ischemia, mediating cardioprotective events. The plasma of birds lacks two key components of the kallikrein-kinin system: the low molecular weight kininogen and a prekallikrein activator analogous to mammalian factor XII, but treatment with bovine plasma kallikrein generates ornitho-kinin [Thr6,Leu8]-BK. The possible cardioprotective effect of ornitho-kinin infusion was investigated in an anesthetized, open-chest chicken model of acute coronary occlusion. A branch of the left main coronary artery was reversibly ligated to produce ischemia followed by reperfusion, after which the degree of myocardial necrosis (infarct size as a percent of area at risk) was assessed by tetrazolium staining. The iv injection of a low dose of ornitho-kinin (4 µg/kg) reduced mean arterial pressure from 88 ± 12 to 42 ± 7 mmHg and increased heart rate from 335 ± 38 to 402 ± 45 bpm (N = 5). The size of the infarct was reduced by pretreatment with ornitho-kinin (500 µg/kg infused over a period of 5 min) from 35 ± 3 to 10 ± 2% of the area at risk. These results suggest that the physiological role of the kallikrein-kinin system is preserved in this animal model in spite of the absence of two key components, i.e., low molecular weight kininogen and factor XII.Instituto Butantan Laboratório de FarmacologiaUniversidade de São Paulo Instituto de Ciências Biomédicas I Departamento de Fisiologia e BiofísicaUniversidade Federal de São Paulo (UNIFESP) Fisiologia Cardiovascular Departamento de FisiologiaUNIFESP, Fisiologia Cardiovascular Depto. de FisiologiaSciELOAssociação Brasileira de Divulgação CientíficaInstituto Butantan Laboratório de FarmacologiaUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Prezoto, Benedito CarlosCouto, Gisele KrugerRossoni, Luciana VenturiniSchoorlemmer, Gerhardus Hermanus Maria [UNIFESP]Carillo, Bruno A. [UNIFESP]Campos, Ruy Ribeiro [UNIFESP]2015-06-14T13:41:10Z2015-06-14T13:41:10Z2009-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion824-830application/pdfhttp://dx.doi.org/10.1590/S0100-879X2009000900009Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 42, n. 9, p. 824-830, 2009.10.1590/S0100-879X2009000900009S0100-879X2009000900009.pdf0100-879XS0100-879X2009000900009http://repositorio.unifesp.br/handle/11600/5247WOS:000270233200009engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T18:34:06Zoai:repositorio.unifesp.br/:11600/5247Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T18:34:06Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion |
title |
Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion |
spellingShingle |
Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion Prezoto, Benedito Carlos Chicken Infarct size Kallikrein-kinin system Ornitho-kinin Factor XII |
title_short |
Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion |
title_full |
Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion |
title_fullStr |
Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion |
title_full_unstemmed |
Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion |
title_sort |
Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion |
author |
Prezoto, Benedito Carlos |
author_facet |
Prezoto, Benedito Carlos Couto, Gisele Kruger Rossoni, Luciana Venturini Schoorlemmer, Gerhardus Hermanus Maria [UNIFESP] Carillo, Bruno A. [UNIFESP] Campos, Ruy Ribeiro [UNIFESP] |
author_role |
author |
author2 |
Couto, Gisele Kruger Rossoni, Luciana Venturini Schoorlemmer, Gerhardus Hermanus Maria [UNIFESP] Carillo, Bruno A. [UNIFESP] Campos, Ruy Ribeiro [UNIFESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Instituto Butantan Laboratório de Farmacologia Universidade de São Paulo (USP) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Prezoto, Benedito Carlos Couto, Gisele Kruger Rossoni, Luciana Venturini Schoorlemmer, Gerhardus Hermanus Maria [UNIFESP] Carillo, Bruno A. [UNIFESP] Campos, Ruy Ribeiro [UNIFESP] |
dc.subject.por.fl_str_mv |
Chicken Infarct size Kallikrein-kinin system Ornitho-kinin Factor XII |
topic |
Chicken Infarct size Kallikrein-kinin system Ornitho-kinin Factor XII |
description |
The generation of bradykinin (BK; Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg) in blood and kallidin (Lys-BK) in tissues by the action of the kallikrein-kinin system has received little attention in non-mammalian vertebrates. In mammals, kallidin can be generated by the coronary endothelium and myocytes in response to ischemia, mediating cardioprotective events. The plasma of birds lacks two key components of the kallikrein-kinin system: the low molecular weight kininogen and a prekallikrein activator analogous to mammalian factor XII, but treatment with bovine plasma kallikrein generates ornitho-kinin [Thr6,Leu8]-BK. The possible cardioprotective effect of ornitho-kinin infusion was investigated in an anesthetized, open-chest chicken model of acute coronary occlusion. A branch of the left main coronary artery was reversibly ligated to produce ischemia followed by reperfusion, after which the degree of myocardial necrosis (infarct size as a percent of area at risk) was assessed by tetrazolium staining. The iv injection of a low dose of ornitho-kinin (4 µg/kg) reduced mean arterial pressure from 88 ± 12 to 42 ± 7 mmHg and increased heart rate from 335 ± 38 to 402 ± 45 bpm (N = 5). The size of the infarct was reduced by pretreatment with ornitho-kinin (500 µg/kg infused over a period of 5 min) from 35 ± 3 to 10 ± 2% of the area at risk. These results suggest that the physiological role of the kallikrein-kinin system is preserved in this animal model in spite of the absence of two key components, i.e., low molecular weight kininogen and factor XII. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09-01 2015-06-14T13:41:10Z 2015-06-14T13:41:10Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-879X2009000900009 Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 42, n. 9, p. 824-830, 2009. 10.1590/S0100-879X2009000900009 S0100-879X2009000900009.pdf 0100-879X S0100-879X2009000900009 http://repositorio.unifesp.br/handle/11600/5247 WOS:000270233200009 |
url |
http://dx.doi.org/10.1590/S0100-879X2009000900009 http://repositorio.unifesp.br/handle/11600/5247 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 42, n. 9, p. 824-830, 2009. 10.1590/S0100-879X2009000900009 S0100-879X2009000900009.pdf 0100-879X S0100-879X2009000900009 WOS:000270233200009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
824-830 application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268287180079104 |