Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion

Detalhes bibliográficos
Autor(a) principal: Prezoto, Benedito Carlos
Data de Publicação: 2009
Outros Autores: Couto, Gisele Kruger, Rossoni, Luciana Venturini, Schoorlemmer, Gerhardus Hermanus Maria [UNIFESP], Carillo, Bruno A. [UNIFESP], Campos, Ruy Ribeiro [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0100-879X2009000900009
http://repositorio.unifesp.br/handle/11600/5247
Resumo: The generation of bradykinin (BK; Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg) in blood and kallidin (Lys-BK) in tissues by the action of the kallikrein-kinin system has received little attention in non-mammalian vertebrates. In mammals, kallidin can be generated by the coronary endothelium and myocytes in response to ischemia, mediating cardioprotective events. The plasma of birds lacks two key components of the kallikrein-kinin system: the low molecular weight kininogen and a prekallikrein activator analogous to mammalian factor XII, but treatment with bovine plasma kallikrein generates ornitho-kinin [Thr6,Leu8]-BK. The possible cardioprotective effect of ornitho-kinin infusion was investigated in an anesthetized, open-chest chicken model of acute coronary occlusion. A branch of the left main coronary artery was reversibly ligated to produce ischemia followed by reperfusion, after which the degree of myocardial necrosis (infarct size as a percent of area at risk) was assessed by tetrazolium staining. The iv injection of a low dose of ornitho-kinin (4 µg/kg) reduced mean arterial pressure from 88 ± 12 to 42 ± 7 mmHg and increased heart rate from 335 ± 38 to 402 ± 45 bpm (N = 5). The size of the infarct was reduced by pretreatment with ornitho-kinin (500 µg/kg infused over a period of 5 min) from 35 ± 3 to 10 ± 2% of the area at risk. These results suggest that the physiological role of the kallikrein-kinin system is preserved in this animal model in spite of the absence of two key components, i.e., low molecular weight kininogen and factor XII.
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spelling Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusionChickenInfarct sizeKallikrein-kinin systemOrnitho-kininFactor XIIThe generation of bradykinin (BK; Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg) in blood and kallidin (Lys-BK) in tissues by the action of the kallikrein-kinin system has received little attention in non-mammalian vertebrates. In mammals, kallidin can be generated by the coronary endothelium and myocytes in response to ischemia, mediating cardioprotective events. The plasma of birds lacks two key components of the kallikrein-kinin system: the low molecular weight kininogen and a prekallikrein activator analogous to mammalian factor XII, but treatment with bovine plasma kallikrein generates ornitho-kinin [Thr6,Leu8]-BK. The possible cardioprotective effect of ornitho-kinin infusion was investigated in an anesthetized, open-chest chicken model of acute coronary occlusion. A branch of the left main coronary artery was reversibly ligated to produce ischemia followed by reperfusion, after which the degree of myocardial necrosis (infarct size as a percent of area at risk) was assessed by tetrazolium staining. The iv injection of a low dose of ornitho-kinin (4 µg/kg) reduced mean arterial pressure from 88 ± 12 to 42 ± 7 mmHg and increased heart rate from 335 ± 38 to 402 ± 45 bpm (N = 5). The size of the infarct was reduced by pretreatment with ornitho-kinin (500 µg/kg infused over a period of 5 min) from 35 ± 3 to 10 ± 2% of the area at risk. These results suggest that the physiological role of the kallikrein-kinin system is preserved in this animal model in spite of the absence of two key components, i.e., low molecular weight kininogen and factor XII.Instituto Butantan Laboratório de FarmacologiaUniversidade de São Paulo Instituto de Ciências Biomédicas I Departamento de Fisiologia e BiofísicaUniversidade Federal de São Paulo (UNIFESP) Fisiologia Cardiovascular Departamento de FisiologiaUNIFESP, Fisiologia Cardiovascular Depto. de FisiologiaSciELOAssociação Brasileira de Divulgação CientíficaInstituto Butantan Laboratório de FarmacologiaUniversidade de São Paulo (USP)Universidade Federal de São Paulo (UNIFESP)Prezoto, Benedito CarlosCouto, Gisele KrugerRossoni, Luciana VenturiniSchoorlemmer, Gerhardus Hermanus Maria [UNIFESP]Carillo, Bruno A. [UNIFESP]Campos, Ruy Ribeiro [UNIFESP]2015-06-14T13:41:10Z2015-06-14T13:41:10Z2009-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion824-830application/pdfhttp://dx.doi.org/10.1590/S0100-879X2009000900009Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 42, n. 9, p. 824-830, 2009.10.1590/S0100-879X2009000900009S0100-879X2009000900009.pdf0100-879XS0100-879X2009000900009http://repositorio.unifesp.br/handle/11600/5247WOS:000270233200009engBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T18:34:06Zoai:repositorio.unifesp.br/:11600/5247Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T18:34:06Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion
title Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion
spellingShingle Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion
Prezoto, Benedito Carlos
Chicken
Infarct size
Kallikrein-kinin system
Ornitho-kinin
Factor XII
title_short Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion
title_full Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion
title_fullStr Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion
title_full_unstemmed Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion
title_sort Cardioprotective effect of ornitho-kinin in an anesthetized, open-chest chicken model of acute coronary occlusion
author Prezoto, Benedito Carlos
author_facet Prezoto, Benedito Carlos
Couto, Gisele Kruger
Rossoni, Luciana Venturini
Schoorlemmer, Gerhardus Hermanus Maria [UNIFESP]
Carillo, Bruno A. [UNIFESP]
Campos, Ruy Ribeiro [UNIFESP]
author_role author
author2 Couto, Gisele Kruger
Rossoni, Luciana Venturini
Schoorlemmer, Gerhardus Hermanus Maria [UNIFESP]
Carillo, Bruno A. [UNIFESP]
Campos, Ruy Ribeiro [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Butantan Laboratório de Farmacologia
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Prezoto, Benedito Carlos
Couto, Gisele Kruger
Rossoni, Luciana Venturini
Schoorlemmer, Gerhardus Hermanus Maria [UNIFESP]
Carillo, Bruno A. [UNIFESP]
Campos, Ruy Ribeiro [UNIFESP]
dc.subject.por.fl_str_mv Chicken
Infarct size
Kallikrein-kinin system
Ornitho-kinin
Factor XII
topic Chicken
Infarct size
Kallikrein-kinin system
Ornitho-kinin
Factor XII
description The generation of bradykinin (BK; Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg) in blood and kallidin (Lys-BK) in tissues by the action of the kallikrein-kinin system has received little attention in non-mammalian vertebrates. In mammals, kallidin can be generated by the coronary endothelium and myocytes in response to ischemia, mediating cardioprotective events. The plasma of birds lacks two key components of the kallikrein-kinin system: the low molecular weight kininogen and a prekallikrein activator analogous to mammalian factor XII, but treatment with bovine plasma kallikrein generates ornitho-kinin [Thr6,Leu8]-BK. The possible cardioprotective effect of ornitho-kinin infusion was investigated in an anesthetized, open-chest chicken model of acute coronary occlusion. A branch of the left main coronary artery was reversibly ligated to produce ischemia followed by reperfusion, after which the degree of myocardial necrosis (infarct size as a percent of area at risk) was assessed by tetrazolium staining. The iv injection of a low dose of ornitho-kinin (4 µg/kg) reduced mean arterial pressure from 88 ± 12 to 42 ± 7 mmHg and increased heart rate from 335 ± 38 to 402 ± 45 bpm (N = 5). The size of the infarct was reduced by pretreatment with ornitho-kinin (500 µg/kg infused over a period of 5 min) from 35 ± 3 to 10 ± 2% of the area at risk. These results suggest that the physiological role of the kallikrein-kinin system is preserved in this animal model in spite of the absence of two key components, i.e., low molecular weight kininogen and factor XII.
publishDate 2009
dc.date.none.fl_str_mv 2009-09-01
2015-06-14T13:41:10Z
2015-06-14T13:41:10Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-879X2009000900009
Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 42, n. 9, p. 824-830, 2009.
10.1590/S0100-879X2009000900009
S0100-879X2009000900009.pdf
0100-879X
S0100-879X2009000900009
http://repositorio.unifesp.br/handle/11600/5247
WOS:000270233200009
url http://dx.doi.org/10.1590/S0100-879X2009000900009
http://repositorio.unifesp.br/handle/11600/5247
identifier_str_mv Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 42, n. 9, p. 824-830, 2009.
10.1590/S0100-879X2009000900009
S0100-879X2009000900009.pdf
0100-879X
S0100-879X2009000900009
WOS:000270233200009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Medical and Biological Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 824-830
application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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