Morphological changes in distant muscle fibers following thermal injury in Wistar rats
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://dx.doi.org/10.1590/S0102-86502010000600012 https://repositorio.unifesp.br/handle/11600/41980 |
Resumo: | Purpose: Thermal injury causes catabolic processes as the body attempts to repair the damaged area. This study evaluated the effects of a scald injury on the morphology of muscle fibers belonging to a muscle distant from the lesion. Methods: Thirty Wistar rats were divided into control (C) and scalded (S) groups. Group S was scalded over 45% of the body surface, standardized by body weight. Rats in both groups were euthanized at four, seven and 14 days following the injury. The middle portions of the medial gastrocnemius muscles were sectioned, stained with hematoxylin and eosin and Picrosirius, and submitted to histological analysis. Results: Control group sections exhibited equidistantly distributed polygonal muscle fibers with peripheral nuclei, characteristic of normal muscle. The injured group sections did not consistently show these characteristics; many fibers in these sections exhibited a rounded contour, variable stain intensities, and greater interfiber distances. A substantially increased amount of connective tissue was also observed on the injured group sections. Conclusion: This experimental model found a morphological change in muscle distant from the site of thermal injury covering 45% of the body surface. |
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Morphological changes in distant muscle fibers following thermal injury in Wistar ratsAlterações morfológicas das fibras musculares de um músculo distante da lesão térmica em ratos WistarMorphologyMuscle Fibers, SkeletalConnective TissueBurnsRatsPurpose: Thermal injury causes catabolic processes as the body attempts to repair the damaged area. This study evaluated the effects of a scald injury on the morphology of muscle fibers belonging to a muscle distant from the lesion. Methods: Thirty Wistar rats were divided into control (C) and scalded (S) groups. Group S was scalded over 45% of the body surface, standardized by body weight. Rats in both groups were euthanized at four, seven and 14 days following the injury. The middle portions of the medial gastrocnemius muscles were sectioned, stained with hematoxylin and eosin and Picrosirius, and submitted to histological analysis. Results: Control group sections exhibited equidistantly distributed polygonal muscle fibers with peripheral nuclei, characteristic of normal muscle. The injured group sections did not consistently show these characteristics; many fibers in these sections exhibited a rounded contour, variable stain intensities, and greater interfiber distances. A substantially increased amount of connective tissue was also observed on the injured group sections. Conclusion: This experimental model found a morphological change in muscle distant from the site of thermal injury covering 45% of the body surface.UNIFESP, Dept Biociencias, BR-11060001 Santos, SP, BrazilUniv Sao Paulo, Sch Med, BR-09500900 Sao Paulo, BrazilState Univ Sao Paulo UNESP, Dept Biosci, Sao Paulo, BrazilUniv Sao Paulo, ICB, Dept Anat, BR-09500900 Sao Paulo, BrazilUniv Sao Paulo, Inst Biomed Sci, Lab Funct Anat Appl Clin & Surg, BR-09500900 Sao Paulo, BrazilUNIFESP, Dept Biociencias, BR-11060001 Santos, SP, BrazilWeb of ScienceActa Cirurgica BrasileiraUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)De Oliveira, Flavia [UNIFESP]Bevilacqua, Luci RezendeAnaruma, Carlos AlbertoBoldrini, Silvia de CamposLiberti, Edson Aparecido2018-06-15T11:14:55Z2018-06-15T11:14:55Z2010-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion525-528https://dx.doi.org/10.1590/S0102-86502010000600012Acta Cirurgica Brasileira. Sao Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 525-528, 2010.10.1590/S0102-86502010000600012S0102-86502010000600012.pdf0102-8650S0102-86502010000600012https://repositorio.unifesp.br/handle/11600/41980WOS:000284779000012engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-09T09:22:17Zoai:repositorio.unifesp.br/:11600/41980Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-09T09:22:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Morphological changes in distant muscle fibers following thermal injury in Wistar rats Alterações morfológicas das fibras musculares de um músculo distante da lesão térmica em ratos Wistar |
title |
Morphological changes in distant muscle fibers following thermal injury in Wistar rats |
spellingShingle |
Morphological changes in distant muscle fibers following thermal injury in Wistar rats De Oliveira, Flavia [UNIFESP] Morphology Muscle Fibers, Skeletal Connective Tissue Burns Rats |
title_short |
Morphological changes in distant muscle fibers following thermal injury in Wistar rats |
title_full |
Morphological changes in distant muscle fibers following thermal injury in Wistar rats |
title_fullStr |
Morphological changes in distant muscle fibers following thermal injury in Wistar rats |
title_full_unstemmed |
Morphological changes in distant muscle fibers following thermal injury in Wistar rats |
title_sort |
Morphological changes in distant muscle fibers following thermal injury in Wistar rats |
author |
De Oliveira, Flavia [UNIFESP] |
author_facet |
De Oliveira, Flavia [UNIFESP] Bevilacqua, Luci Rezende Anaruma, Carlos Alberto Boldrini, Silvia de Campos Liberti, Edson Aparecido |
author_role |
author |
author2 |
Bevilacqua, Luci Rezende Anaruma, Carlos Alberto Boldrini, Silvia de Campos Liberti, Edson Aparecido |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
De Oliveira, Flavia [UNIFESP] Bevilacqua, Luci Rezende Anaruma, Carlos Alberto Boldrini, Silvia de Campos Liberti, Edson Aparecido |
dc.subject.por.fl_str_mv |
Morphology Muscle Fibers, Skeletal Connective Tissue Burns Rats |
topic |
Morphology Muscle Fibers, Skeletal Connective Tissue Burns Rats |
description |
Purpose: Thermal injury causes catabolic processes as the body attempts to repair the damaged area. This study evaluated the effects of a scald injury on the morphology of muscle fibers belonging to a muscle distant from the lesion. Methods: Thirty Wistar rats were divided into control (C) and scalded (S) groups. Group S was scalded over 45% of the body surface, standardized by body weight. Rats in both groups were euthanized at four, seven and 14 days following the injury. The middle portions of the medial gastrocnemius muscles were sectioned, stained with hematoxylin and eosin and Picrosirius, and submitted to histological analysis. Results: Control group sections exhibited equidistantly distributed polygonal muscle fibers with peripheral nuclei, characteristic of normal muscle. The injured group sections did not consistently show these characteristics; many fibers in these sections exhibited a rounded contour, variable stain intensities, and greater interfiber distances. A substantially increased amount of connective tissue was also observed on the injured group sections. Conclusion: This experimental model found a morphological change in muscle distant from the site of thermal injury covering 45% of the body surface. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-11-01 2018-06-15T11:14:55Z 2018-06-15T11:14:55Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://dx.doi.org/10.1590/S0102-86502010000600012 Acta Cirurgica Brasileira. Sao Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 525-528, 2010. 10.1590/S0102-86502010000600012 S0102-86502010000600012.pdf 0102-8650 S0102-86502010000600012 https://repositorio.unifesp.br/handle/11600/41980 WOS:000284779000012 |
url |
https://dx.doi.org/10.1590/S0102-86502010000600012 https://repositorio.unifesp.br/handle/11600/41980 |
identifier_str_mv |
Acta Cirurgica Brasileira. Sao Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 525-528, 2010. 10.1590/S0102-86502010000600012 S0102-86502010000600012.pdf 0102-8650 S0102-86502010000600012 WOS:000284779000012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
525-528 |
dc.publisher.none.fl_str_mv |
Acta Cirurgica Brasileira |
publisher.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268367104638976 |