Morphological changes in distant muscle fibers following thermal injury in Wistar rats

Detalhes bibliográficos
Autor(a) principal: De Oliveira, Flavia [UNIFESP]
Data de Publicação: 2010
Outros Autores: Bevilacqua, Luci Rezende, Anaruma, Carlos Alberto, Boldrini, Silvia de Campos, Liberti, Edson Aparecido
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: https://dx.doi.org/10.1590/S0102-86502010000600012
https://repositorio.unifesp.br/handle/11600/41980
Resumo: Purpose: Thermal injury causes catabolic processes as the body attempts to repair the damaged area. This study evaluated the effects of a scald injury on the morphology of muscle fibers belonging to a muscle distant from the lesion. Methods: Thirty Wistar rats were divided into control (C) and scalded (S) groups. Group S was scalded over 45% of the body surface, standardized by body weight. Rats in both groups were euthanized at four, seven and 14 days following the injury. The middle portions of the medial gastrocnemius muscles were sectioned, stained with hematoxylin and eosin and Picrosirius, and submitted to histological analysis. Results: Control group sections exhibited equidistantly distributed polygonal muscle fibers with peripheral nuclei, characteristic of normal muscle. The injured group sections did not consistently show these characteristics; many fibers in these sections exhibited a rounded contour, variable stain intensities, and greater interfiber distances. A substantially increased amount of connective tissue was also observed on the injured group sections. Conclusion: This experimental model found a morphological change in muscle distant from the site of thermal injury covering 45% of the body surface.
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spelling Morphological changes in distant muscle fibers following thermal injury in Wistar ratsAlterações morfológicas das fibras musculares de um músculo distante da lesão térmica em ratos WistarMorphologyMuscle Fibers, SkeletalConnective TissueBurnsRatsPurpose: Thermal injury causes catabolic processes as the body attempts to repair the damaged area. This study evaluated the effects of a scald injury on the morphology of muscle fibers belonging to a muscle distant from the lesion. Methods: Thirty Wistar rats were divided into control (C) and scalded (S) groups. Group S was scalded over 45% of the body surface, standardized by body weight. Rats in both groups were euthanized at four, seven and 14 days following the injury. The middle portions of the medial gastrocnemius muscles were sectioned, stained with hematoxylin and eosin and Picrosirius, and submitted to histological analysis. Results: Control group sections exhibited equidistantly distributed polygonal muscle fibers with peripheral nuclei, characteristic of normal muscle. The injured group sections did not consistently show these characteristics; many fibers in these sections exhibited a rounded contour, variable stain intensities, and greater interfiber distances. A substantially increased amount of connective tissue was also observed on the injured group sections. Conclusion: This experimental model found a morphological change in muscle distant from the site of thermal injury covering 45% of the body surface.UNIFESP, Dept Biociencias, BR-11060001 Santos, SP, BrazilUniv Sao Paulo, Sch Med, BR-09500900 Sao Paulo, BrazilState Univ Sao Paulo UNESP, Dept Biosci, Sao Paulo, BrazilUniv Sao Paulo, ICB, Dept Anat, BR-09500900 Sao Paulo, BrazilUniv Sao Paulo, Inst Biomed Sci, Lab Funct Anat Appl Clin & Surg, BR-09500900 Sao Paulo, BrazilUNIFESP, Dept Biociencias, BR-11060001 Santos, SP, BrazilWeb of ScienceActa Cirurgica BrasileiraUniversidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)De Oliveira, Flavia [UNIFESP]Bevilacqua, Luci RezendeAnaruma, Carlos AlbertoBoldrini, Silvia de CamposLiberti, Edson Aparecido2018-06-15T11:14:55Z2018-06-15T11:14:55Z2010-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion525-528https://dx.doi.org/10.1590/S0102-86502010000600012Acta Cirurgica Brasileira. Sao Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 525-528, 2010.10.1590/S0102-86502010000600012S0102-86502010000600012.pdf0102-8650S0102-86502010000600012https://repositorio.unifesp.br/handle/11600/41980WOS:000284779000012engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-09T09:22:17Zoai:repositorio.unifesp.br/:11600/41980Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-09T09:22:17Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Morphological changes in distant muscle fibers following thermal injury in Wistar rats
Alterações morfológicas das fibras musculares de um músculo distante da lesão térmica em ratos Wistar
title Morphological changes in distant muscle fibers following thermal injury in Wistar rats
spellingShingle Morphological changes in distant muscle fibers following thermal injury in Wistar rats
De Oliveira, Flavia [UNIFESP]
Morphology
Muscle Fibers, Skeletal
Connective Tissue
Burns
Rats
title_short Morphological changes in distant muscle fibers following thermal injury in Wistar rats
title_full Morphological changes in distant muscle fibers following thermal injury in Wistar rats
title_fullStr Morphological changes in distant muscle fibers following thermal injury in Wistar rats
title_full_unstemmed Morphological changes in distant muscle fibers following thermal injury in Wistar rats
title_sort Morphological changes in distant muscle fibers following thermal injury in Wistar rats
author De Oliveira, Flavia [UNIFESP]
author_facet De Oliveira, Flavia [UNIFESP]
Bevilacqua, Luci Rezende
Anaruma, Carlos Alberto
Boldrini, Silvia de Campos
Liberti, Edson Aparecido
author_role author
author2 Bevilacqua, Luci Rezende
Anaruma, Carlos Alberto
Boldrini, Silvia de Campos
Liberti, Edson Aparecido
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv De Oliveira, Flavia [UNIFESP]
Bevilacqua, Luci Rezende
Anaruma, Carlos Alberto
Boldrini, Silvia de Campos
Liberti, Edson Aparecido
dc.subject.por.fl_str_mv Morphology
Muscle Fibers, Skeletal
Connective Tissue
Burns
Rats
topic Morphology
Muscle Fibers, Skeletal
Connective Tissue
Burns
Rats
description Purpose: Thermal injury causes catabolic processes as the body attempts to repair the damaged area. This study evaluated the effects of a scald injury on the morphology of muscle fibers belonging to a muscle distant from the lesion. Methods: Thirty Wistar rats were divided into control (C) and scalded (S) groups. Group S was scalded over 45% of the body surface, standardized by body weight. Rats in both groups were euthanized at four, seven and 14 days following the injury. The middle portions of the medial gastrocnemius muscles were sectioned, stained with hematoxylin and eosin and Picrosirius, and submitted to histological analysis. Results: Control group sections exhibited equidistantly distributed polygonal muscle fibers with peripheral nuclei, characteristic of normal muscle. The injured group sections did not consistently show these characteristics; many fibers in these sections exhibited a rounded contour, variable stain intensities, and greater interfiber distances. A substantially increased amount of connective tissue was also observed on the injured group sections. Conclusion: This experimental model found a morphological change in muscle distant from the site of thermal injury covering 45% of the body surface.
publishDate 2010
dc.date.none.fl_str_mv 2010-11-01
2018-06-15T11:14:55Z
2018-06-15T11:14:55Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://dx.doi.org/10.1590/S0102-86502010000600012
Acta Cirurgica Brasileira. Sao Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 525-528, 2010.
10.1590/S0102-86502010000600012
S0102-86502010000600012.pdf
0102-8650
S0102-86502010000600012
https://repositorio.unifesp.br/handle/11600/41980
WOS:000284779000012
url https://dx.doi.org/10.1590/S0102-86502010000600012
https://repositorio.unifesp.br/handle/11600/41980
identifier_str_mv Acta Cirurgica Brasileira. Sao Paulo: Acta Cirurgica Brasileira, v. 25, n. 6, p. 525-528, 2010.
10.1590/S0102-86502010000600012
S0102-86502010000600012.pdf
0102-8650
S0102-86502010000600012
WOS:000284779000012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 525-528
dc.publisher.none.fl_str_mv Acta Cirurgica Brasileira
publisher.none.fl_str_mv Acta Cirurgica Brasileira
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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