Relação da densidade tumoral das células Dendríticas e Natural Killer com os aspectos clínico-patológicos e prognóstico no adenocarcinoma gástrico

• Autor(a) principal: Rosso, Déborah de [UNIFESP]
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: https://repositorio.unifesp.br/handle/11600/10023
• topic: Células matadoras naturais; Células dendríticas; Prognóstico; Sobrevivência; Neoplasias gástricas

Introduction: Dendritic (DC) and Natural Killer (NK) cells are responsible for individual’s natural immunity and more and more its presence infiltrating the tumors is being related with clinical and pathological features in different types of tumors, including Gastric Cancer. Objectives: Evaluate the density of dendritic and natural killer cells in gastric adenocarcinoma and its relation with several prognostic factors and with survival. Methods: We studied 72 cases of gastric adenocarcinoma submitted to gastrectomy with D2 lymph node dissection in the Service of General Surgery of The University of Santo Amaro, between 1997 and 2007. We analyzed age, gender, tumor location in the stomach, degree of differentiation (well, moderately or poorly differentiated types), Laurén’s classification, tumor stage, lymph node invasion, metastasis and survival. The evaluation of dendritic cells was done through immunohistochemical evaluation with CD1a and CD57. Ten high power (400x) fields (HPF) were analyzed and the patients divided into 2 groups: those with high cell density (> 15 cells/10 HPF) and those with low cell density (< 15 cells/10 HPF). Results: Regarding the density of DC there was a very small percentage of patients (5%) with high density; this finding did not allow a statistical evaluation. There was no correlation of NK with the several findings studied: gender, age, location, tumor depth (T), lymph node invasion (N), metastasis (M) and cell differentiation. In more advanced stages the NK cells density was significantly higher (p<0.02). The survival was higher in the group with high density of NK when compared with the low-density group (32.25 versus 18.22 months) (p=0.0007). Conclusions: The analysis of the several clinical, surgical and pathological findings did not show difference in Natural Killer cells density. Only in advanced stages the density of NK cells was significantly higher. Patients with high NK cells density presented higher survival rates when compared to those with low density.