Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://dx.doi.org/10.2108/zs140005 http://repositorio.unifesp.br/handle/11600/38133 |
Resumo: | Buthid scorpions exhibit a high variability in diploid number within genera and even within species. Cytogenetically, Buthidae differs from other families of Scorpiones based on its low diploid numbers, holocentric chromosomes, and complex chromosomal chains, which form during meiosis. in this study, we analyzed the distribution of the 45S ribosomal DNA (rDNA) genes in the mitotic and meiotic chromosomes of seven buthid species belonging to the genera Rhopalurus and Tityus with the ultimate goal of elucidating the chromosome organization in these scorpions. the chromosome number ranged from 2n = 6 to 2n = 28. Despite the high variance in diploid number, all species examined carried their 45S rDNA sites in the terminal region of exactly two chromosomes. Analyses of meiotic cells revealed 45S rDNA clusters in the chromosomal chains of Rhopalurus agamemnon, Tityus bahiensis, Tityus confluens, and Tityus martinpaechi, or in bivalent-like configuration in Rhopalurus rochai, Tityus bahiensis, Tityus confluens, Tityus fasciolatus, and Tityus paraguayensis. in the species examined, the 45S rDNA sites colocalized with constitutive heterochromatin regions. in light of the high chromosome variability and maintenance of number and terminal position of 45S rDNA sites in buthids, the heterochromatin may act to conserve the integrity of the ribosomal genes. |
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Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpionsconstitutive heterochromatinchromosome chaindiploid numberfluorescent in situ hybridizationholocentric chromosomesrDNAButhid scorpions exhibit a high variability in diploid number within genera and even within species. Cytogenetically, Buthidae differs from other families of Scorpiones based on its low diploid numbers, holocentric chromosomes, and complex chromosomal chains, which form during meiosis. in this study, we analyzed the distribution of the 45S ribosomal DNA (rDNA) genes in the mitotic and meiotic chromosomes of seven buthid species belonging to the genera Rhopalurus and Tityus with the ultimate goal of elucidating the chromosome organization in these scorpions. the chromosome number ranged from 2n = 6 to 2n = 28. Despite the high variance in diploid number, all species examined carried their 45S rDNA sites in the terminal region of exactly two chromosomes. Analyses of meiotic cells revealed 45S rDNA clusters in the chromosomal chains of Rhopalurus agamemnon, Tityus bahiensis, Tityus confluens, and Tityus martinpaechi, or in bivalent-like configuration in Rhopalurus rochai, Tityus bahiensis, Tityus confluens, Tityus fasciolatus, and Tityus paraguayensis. in the species examined, the 45S rDNA sites colocalized with constitutive heterochromatin regions. in light of the high chromosome variability and maintenance of number and terminal position of 45S rDNA sites in buthids, the heterochromatin may act to conserve the integrity of the ribosomal genes.Univ Estadual Paulista, UNESP, Dept Biol, Inst Biociencias, BR-13506900 São Paulo, BrazilUniv Fed Piaui, UFPI, BR-64800000 Floriano, Piaui, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Programa Posgrad Zool, Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, UNIFESP, BR-09972270 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, UNIFESP, BR-09972270 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Programa de Pesquisas em Biodiversidade do Semiarido - PPBio SemiaridoFAPESP: 2010/14226-2FAPESP: 2011/21643-1CNPq: 478630/2010-7Programa de Pesquisas em Biodiversidade do Semiarido - PPBio Semiarido: CNPq 558317/2009-0Programa de Pesquisas em Biodiversidade do Semiarido - PPBio Semiarido: 457471/2012-3Zoological Soc JapanUniv Estadual PaulistaUniv Fed PiauiUniversidade Federal de Minas Gerais (UFMG)Universidade Federal de São Paulo (UNIFESP)Mattos, Viviane FagundesCarvalho, Leonardo SousaCella, Doralice MariaSchneider, Marielle Cristina [UNIFESP]2016-01-24T14:37:46Z2016-01-24T14:37:46Z2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion603-607http://dx.doi.org/10.2108/zs140005Zoological Science. Tokyo: Zoological Soc Japan, v. 31, n. 9, p. 603-607, 2014.10.2108/zs1400050289-0003http://repositorio.unifesp.br/handle/11600/38133WOS:000341573400008engZoological Scienceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-09-28T17:04:12Zoai:repositorio.unifesp.br/:11600/38133Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652021-09-28T17:04:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions |
title |
Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions |
spellingShingle |
Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions Mattos, Viviane Fagundes constitutive heterochromatin chromosome chain diploid number fluorescent in situ hybridization holocentric chromosomes rDNA |
title_short |
Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions |
title_full |
Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions |
title_fullStr |
Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions |
title_full_unstemmed |
Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions |
title_sort |
Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions |
author |
Mattos, Viviane Fagundes |
author_facet |
Mattos, Viviane Fagundes Carvalho, Leonardo Sousa Cella, Doralice Maria Schneider, Marielle Cristina [UNIFESP] |
author_role |
author |
author2 |
Carvalho, Leonardo Sousa Cella, Doralice Maria Schneider, Marielle Cristina [UNIFESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Univ Estadual Paulista Univ Fed Piaui Universidade Federal de Minas Gerais (UFMG) Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Mattos, Viviane Fagundes Carvalho, Leonardo Sousa Cella, Doralice Maria Schneider, Marielle Cristina [UNIFESP] |
dc.subject.por.fl_str_mv |
constitutive heterochromatin chromosome chain diploid number fluorescent in situ hybridization holocentric chromosomes rDNA |
topic |
constitutive heterochromatin chromosome chain diploid number fluorescent in situ hybridization holocentric chromosomes rDNA |
description |
Buthid scorpions exhibit a high variability in diploid number within genera and even within species. Cytogenetically, Buthidae differs from other families of Scorpiones based on its low diploid numbers, holocentric chromosomes, and complex chromosomal chains, which form during meiosis. in this study, we analyzed the distribution of the 45S ribosomal DNA (rDNA) genes in the mitotic and meiotic chromosomes of seven buthid species belonging to the genera Rhopalurus and Tityus with the ultimate goal of elucidating the chromosome organization in these scorpions. the chromosome number ranged from 2n = 6 to 2n = 28. Despite the high variance in diploid number, all species examined carried their 45S rDNA sites in the terminal region of exactly two chromosomes. Analyses of meiotic cells revealed 45S rDNA clusters in the chromosomal chains of Rhopalurus agamemnon, Tityus bahiensis, Tityus confluens, and Tityus martinpaechi, or in bivalent-like configuration in Rhopalurus rochai, Tityus bahiensis, Tityus confluens, Tityus fasciolatus, and Tityus paraguayensis. in the species examined, the 45S rDNA sites colocalized with constitutive heterochromatin regions. in light of the high chromosome variability and maintenance of number and terminal position of 45S rDNA sites in buthids, the heterochromatin may act to conserve the integrity of the ribosomal genes. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-01 2016-01-24T14:37:46Z 2016-01-24T14:37:46Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2108/zs140005 Zoological Science. Tokyo: Zoological Soc Japan, v. 31, n. 9, p. 603-607, 2014. 10.2108/zs140005 0289-0003 http://repositorio.unifesp.br/handle/11600/38133 WOS:000341573400008 |
url |
http://dx.doi.org/10.2108/zs140005 http://repositorio.unifesp.br/handle/11600/38133 |
identifier_str_mv |
Zoological Science. Tokyo: Zoological Soc Japan, v. 31, n. 9, p. 603-607, 2014. 10.2108/zs140005 0289-0003 WOS:000341573400008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zoological Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
603-607 |
dc.publisher.none.fl_str_mv |
Zoological Soc Japan |
publisher.none.fl_str_mv |
Zoological Soc Japan |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268353816035328 |