Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions

Detalhes bibliográficos
Autor(a) principal: Mattos, Viviane Fagundes
Data de Publicação: 2014
Outros Autores: Carvalho, Leonardo Sousa, Cella, Doralice Maria, Schneider, Marielle Cristina [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.2108/zs140005
http://repositorio.unifesp.br/handle/11600/38133
Resumo: Buthid scorpions exhibit a high variability in diploid number within genera and even within species. Cytogenetically, Buthidae differs from other families of Scorpiones based on its low diploid numbers, holocentric chromosomes, and complex chromosomal chains, which form during meiosis. in this study, we analyzed the distribution of the 45S ribosomal DNA (rDNA) genes in the mitotic and meiotic chromosomes of seven buthid species belonging to the genera Rhopalurus and Tityus with the ultimate goal of elucidating the chromosome organization in these scorpions. the chromosome number ranged from 2n = 6 to 2n = 28. Despite the high variance in diploid number, all species examined carried their 45S rDNA sites in the terminal region of exactly two chromosomes. Analyses of meiotic cells revealed 45S rDNA clusters in the chromosomal chains of Rhopalurus agamemnon, Tityus bahiensis, Tityus confluens, and Tityus martinpaechi, or in bivalent-like configuration in Rhopalurus rochai, Tityus bahiensis, Tityus confluens, Tityus fasciolatus, and Tityus paraguayensis. in the species examined, the 45S rDNA sites colocalized with constitutive heterochromatin regions. in light of the high chromosome variability and maintenance of number and terminal position of 45S rDNA sites in buthids, the heterochromatin may act to conserve the integrity of the ribosomal genes.
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spelling Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpionsconstitutive heterochromatinchromosome chaindiploid numberfluorescent in situ hybridizationholocentric chromosomesrDNAButhid scorpions exhibit a high variability in diploid number within genera and even within species. Cytogenetically, Buthidae differs from other families of Scorpiones based on its low diploid numbers, holocentric chromosomes, and complex chromosomal chains, which form during meiosis. in this study, we analyzed the distribution of the 45S ribosomal DNA (rDNA) genes in the mitotic and meiotic chromosomes of seven buthid species belonging to the genera Rhopalurus and Tityus with the ultimate goal of elucidating the chromosome organization in these scorpions. the chromosome number ranged from 2n = 6 to 2n = 28. Despite the high variance in diploid number, all species examined carried their 45S rDNA sites in the terminal region of exactly two chromosomes. Analyses of meiotic cells revealed 45S rDNA clusters in the chromosomal chains of Rhopalurus agamemnon, Tityus bahiensis, Tityus confluens, and Tityus martinpaechi, or in bivalent-like configuration in Rhopalurus rochai, Tityus bahiensis, Tityus confluens, Tityus fasciolatus, and Tityus paraguayensis. in the species examined, the 45S rDNA sites colocalized with constitutive heterochromatin regions. in light of the high chromosome variability and maintenance of number and terminal position of 45S rDNA sites in buthids, the heterochromatin may act to conserve the integrity of the ribosomal genes.Univ Estadual Paulista, UNESP, Dept Biol, Inst Biociencias, BR-13506900 São Paulo, BrazilUniv Fed Piaui, UFPI, BR-64800000 Floriano, Piaui, BrazilUniv Fed Minas Gerais, Inst Ciencias Biol, Programa Posgrad Zool, Belo Horizonte, MG, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, UNIFESP, BR-09972270 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Ciencias Biol, UNIFESP, BR-09972270 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Programa de Pesquisas em Biodiversidade do Semiarido - PPBio SemiaridoFAPESP: 2010/14226-2FAPESP: 2011/21643-1CNPq: 478630/2010-7Programa de Pesquisas em Biodiversidade do Semiarido - PPBio Semiarido: CNPq 558317/2009-0Programa de Pesquisas em Biodiversidade do Semiarido - PPBio Semiarido: 457471/2012-3Zoological Soc JapanUniv Estadual PaulistaUniv Fed PiauiUniversidade Federal de Minas Gerais (UFMG)Universidade Federal de São Paulo (UNIFESP)Mattos, Viviane FagundesCarvalho, Leonardo SousaCella, Doralice MariaSchneider, Marielle Cristina [UNIFESP]2016-01-24T14:37:46Z2016-01-24T14:37:46Z2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion603-607http://dx.doi.org/10.2108/zs140005Zoological Science. Tokyo: Zoological Soc Japan, v. 31, n. 9, p. 603-607, 2014.10.2108/zs1400050289-0003http://repositorio.unifesp.br/handle/11600/38133WOS:000341573400008engZoological Scienceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2021-09-28T17:04:12Zoai:repositorio.unifesp.br/:11600/38133Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652021-09-28T17:04:12Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions
title Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions
spellingShingle Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions
Mattos, Viviane Fagundes
constitutive heterochromatin
chromosome chain
diploid number
fluorescent in situ hybridization
holocentric chromosomes
rDNA
title_short Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions
title_full Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions
title_fullStr Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions
title_full_unstemmed Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions
title_sort Location of 45S Ribosomal Genes in Mitotic and Meiotic Chromosomes of Buthid Scorpions
author Mattos, Viviane Fagundes
author_facet Mattos, Viviane Fagundes
Carvalho, Leonardo Sousa
Cella, Doralice Maria
Schneider, Marielle Cristina [UNIFESP]
author_role author
author2 Carvalho, Leonardo Sousa
Cella, Doralice Maria
Schneider, Marielle Cristina [UNIFESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Univ Estadual Paulista
Univ Fed Piaui
Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Mattos, Viviane Fagundes
Carvalho, Leonardo Sousa
Cella, Doralice Maria
Schneider, Marielle Cristina [UNIFESP]
dc.subject.por.fl_str_mv constitutive heterochromatin
chromosome chain
diploid number
fluorescent in situ hybridization
holocentric chromosomes
rDNA
topic constitutive heterochromatin
chromosome chain
diploid number
fluorescent in situ hybridization
holocentric chromosomes
rDNA
description Buthid scorpions exhibit a high variability in diploid number within genera and even within species. Cytogenetically, Buthidae differs from other families of Scorpiones based on its low diploid numbers, holocentric chromosomes, and complex chromosomal chains, which form during meiosis. in this study, we analyzed the distribution of the 45S ribosomal DNA (rDNA) genes in the mitotic and meiotic chromosomes of seven buthid species belonging to the genera Rhopalurus and Tityus with the ultimate goal of elucidating the chromosome organization in these scorpions. the chromosome number ranged from 2n = 6 to 2n = 28. Despite the high variance in diploid number, all species examined carried their 45S rDNA sites in the terminal region of exactly two chromosomes. Analyses of meiotic cells revealed 45S rDNA clusters in the chromosomal chains of Rhopalurus agamemnon, Tityus bahiensis, Tityus confluens, and Tityus martinpaechi, or in bivalent-like configuration in Rhopalurus rochai, Tityus bahiensis, Tityus confluens, Tityus fasciolatus, and Tityus paraguayensis. in the species examined, the 45S rDNA sites colocalized with constitutive heterochromatin regions. in light of the high chromosome variability and maintenance of number and terminal position of 45S rDNA sites in buthids, the heterochromatin may act to conserve the integrity of the ribosomal genes.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-01
2016-01-24T14:37:46Z
2016-01-24T14:37:46Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.2108/zs140005
Zoological Science. Tokyo: Zoological Soc Japan, v. 31, n. 9, p. 603-607, 2014.
10.2108/zs140005
0289-0003
http://repositorio.unifesp.br/handle/11600/38133
WOS:000341573400008
url http://dx.doi.org/10.2108/zs140005
http://repositorio.unifesp.br/handle/11600/38133
identifier_str_mv Zoological Science. Tokyo: Zoological Soc Japan, v. 31, n. 9, p. 603-607, 2014.
10.2108/zs140005
0289-0003
WOS:000341573400008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Zoological Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 603-607
dc.publisher.none.fl_str_mv Zoological Soc Japan
publisher.none.fl_str_mv Zoological Soc Japan
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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