Effects of ovariectomy on the secretory apparatus in the right atrial cardiomyocytes of middle-aged mice

• Autor(a) principal: Souza, Romeu R. de
• Data de Publicação: 2014
• Outros Autores: Oliveira, Vanessa C. de, Curi, Tania Cristina P., Maldonado, Diogo C. [UNIFESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://dx.doi.org/10.6061/clinics/2014(08)09; http://repositorio.unifesp.br/handle/11600/37198
• topic: Natriuretic Peptide; Estrogen Deficiency; Morphometry

OBJECTIVE : the aim of the present study was to evaluate the effects of ovariectomy on the secretory apparatus of natriuretic peptides in right atrial cardiomyocytes.METHODS: Nine-month-old mice underwent bilateral ovariectomy or sham surgery. the blood exam of the ovariectomized mice showed results consistent with castrated females. Systolic blood pressure was measured after ovariectomy (9 mo of age) and at the moment of sacrifice (12 mo of age). Fragments of the right atrium were collected and prepared for electron microscopy examination. the following variables were quantified: the quantitative density and area of the natriuretic peptide granules, the relative volume of euchromatin in the nucleus, the number of pores per 10 mu m of the nuclear membrane and the relative volumes of the mitochondria and Golgi complex.RESULTS: the cardiomyocytes obtained from ovariectomized mice indicated that the quantitative density and the area of secretory granules of natriuretic peptides were significantly lower compared with the shamoperated mice. Furthermore, there was a decrease in the relative volume of euchromatin, a lower density of nuclear pores, and lower relative volumes of the mitochondria and Golgi complex in the ovariectomized mice compared with the sham-operated mice. These findings suggest a pool with a low turnover rate, i.e., low synthesis and elimination of natriuretic peptides.CONCLUSION: A lack of estrogen caused hypotrophy of the secretory apparatus in right atrial cardiomyocytes that could explain the weak synthesis of natriuretic peptides in mice. Furthermore, one of the mechanisms of blood pressure control was lost, which may explain, in part, the elevated blood pressure in ovariectomized mice.