Lepstospira interrogans shotgun phage display identified LigB as a heparin-binding protein

• Autor(a) principal: Ching Ching, Ana Tung
• Data de Publicação: 2012
• Outros Autores: Favaro, Regiane Degan, Lima, Swiany Silveira, Muniz Chaves, Agtha de Alencar, Lima, Marcelo Andrade de [UNIFESP], Nader, Helena Bonciani [UNIFESP], Estima Abreu, Patricia A., Ho, Paulo Lee
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNIFESP
• Texto Completo: http://repositorio.unifesp.br/handle/11600/35497; http://dx.doi.org/10.1016/j.bbrc.2012.09.137
• topic: Leptospira interrogans; Heparin; Phage display; LigB

LigB is an adhesin from pathogenic Leptospira that is able to bind to extracellular matrix and is considered a virulence factor. A shotgun phage display genomic library was constructed and used for panning against Heparan Sulfate Proteoglycan (HSPG). A phage clone encoding part of LigB protein was selected in panning experiments and showed specific binding to heparin. To validate the selected clone, fragments of LigB were produced as recombinant proteins and showed affinity to heparin and to mammalian cells. Heparin was also able to reduce the binding of rLB-Ct to mammalian cells. Our data suggests that the glycosaminoglycan moiety of the HSPG is responsible for its binding and could mediate the attachment of the recombinant protein rLB-Ct. Thus, heparin may act as a receptor for Leptospira to colonize and to invade the host tissue. (C) 2012 Elsevier Inc. All rights reserved.