pH-Dependent Synthesis of Anisotropic Gold Nanostructures by Bioinspired Cysteine-Containing Peptides
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/51039 http://dx.doi.org/10.1021/acsomega.6b00140 |
Resumo: | In the present study, alkaline peptides AAAXCX (X = lysine or arginine residues) were designed based on the conserved motif of the enzyme thioredoxin and used for the synthesis of gold nanoparticles (GNPs) in the pH range of 2-11. These peptides were compared with free cysteine, the counterpart acidic peptides AAAECE and gamma-ECG (glutathione), and the neutral peptide AAAACA. The objective was to investigate the effect of the amino acids neighboring a cysteine residue on the pH-dependent synthesis of gold nanocrystals. Kohn-Sham density functional theory (KS-DFT) calculations indicated an increase in the reducing capacity of AAAKCK favored by the successive deprotonation of their ionizable groups at increasing pH values. Experimentally, it was observed that gold speciation and the peptide structure also have a strong influence on the synthesis and stabilization of GNPs. AAAKCK produced GNPs at room temperature, in the whole investigated pH range. By contrast, alkaline pH was the best condition for the synthesis of GNP assisted by the AAARCR peptide. The acidic peptides produced GNPs only in the presence of polyethylene glycol, and the synthesis using AAAECE and gamma-ECG also required heating. The ionization state of AAAKCK had a strong influence on the preferential growth of the GNPs. Therefore, pH had a remarkable effect on the synthesis, kinetics, size, shape, and polydispersity of GNPs produced using AAAKCK. The AAAKCK peptide produced anisotropic decahedral and platelike nanocrystals at acidic pH values and spherical GNPs at alkaline pH values. Both alkaline peptides were also efficient capping agents for GNPs, but they produced a significant difference in the zeta potential, probably because of different orientations on the gold surface. |
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Tofanello, AryaneMiranda, Erica G. A.Dias, Igor W. R.Lanfredi, Alexandre J. C.Arantes, Jeverson T.Juliano, Maria A. [UNIFESP]Nantes, Iseli L.2019-07-22T15:46:43Z2019-07-22T15:46:43Z2016Acs Omega. Washington, v. 1, n. 3, p. 424-434, 2016.2470-1343http://repositorio.unifesp.br/handle/11600/51039http://dx.doi.org/10.1021/acsomega.6b00140WOS000391204000013.pdf10.1021/acsomega.6b00140WOS:000391204000013In the present study, alkaline peptides AAAXCX (X = lysine or arginine residues) were designed based on the conserved motif of the enzyme thioredoxin and used for the synthesis of gold nanoparticles (GNPs) in the pH range of 2-11. These peptides were compared with free cysteine, the counterpart acidic peptides AAAECE and gamma-ECG (glutathione), and the neutral peptide AAAACA. The objective was to investigate the effect of the amino acids neighboring a cysteine residue on the pH-dependent synthesis of gold nanocrystals. Kohn-Sham density functional theory (KS-DFT) calculations indicated an increase in the reducing capacity of AAAKCK favored by the successive deprotonation of their ionizable groups at increasing pH values. Experimentally, it was observed that gold speciation and the peptide structure also have a strong influence on the synthesis and stabilization of GNPs. AAAKCK produced GNPs at room temperature, in the whole investigated pH range. By contrast, alkaline pH was the best condition for the synthesis of GNP assisted by the AAARCR peptide. The acidic peptides produced GNPs only in the presence of polyethylene glycol, and the synthesis using AAAECE and gamma-ECG also required heating. The ionization state of AAAKCK had a strong influence on the preferential growth of the GNPs. Therefore, pH had a remarkable effect on the synthesis, kinetics, size, shape, and polydispersity of GNPs produced using AAAKCK. The AAAKCK peptide produced anisotropic decahedral and platelike nanocrystals at acidic pH values and spherical GNPs at alkaline pH values. Both alkaline peptides were also efficient capping agents for GNPs, but they produced a significant difference in the zeta potential, probably because of different orientations on the gold surface.FAPESP [2012/07456-7, 2015/17688-0]CNPqCAPES [5583/2014-04]Univ Fed ABC UFABC, CCNH, NanoBioMAv, Ave Estados 5001, BR-09210580 Sao Paulo, BrazilUniv Fed ABC UFABC, Ctr Engn Modelagem & Ciencias Sociais Aplicadas C, Ave Estados 5001, BR-09210580 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Biol Mol, Rua 3 Maio 100,Vila Clementino, BR-04044020 Sao Paulo, BrazilUniv Fed Sao Paulo, Dept Biol Mol, Rua 3 Maio 100,Vila Clementino, BR-04044020 Sao Paulo, BrazilFAPESP:2012/07456-72015/17688-0CAPES:5583/2014-04Web of Science424-434engAmer Chemical SocpH-Dependent Synthesis of Anisotropic Gold Nanostructures by Bioinspired Cysteine-Containing Peptidesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP11600/510392021-09-29 12:11:15.846metadata only accessoai:repositorio.unifesp.br:11600/51039Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-09-29T15:11:15Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
pH-Dependent Synthesis of Anisotropic Gold Nanostructures by Bioinspired Cysteine-Containing Peptides |
title |
pH-Dependent Synthesis of Anisotropic Gold Nanostructures by Bioinspired Cysteine-Containing Peptides |
spellingShingle |
pH-Dependent Synthesis of Anisotropic Gold Nanostructures by Bioinspired Cysteine-Containing Peptides Tofanello, Aryane |
title_short |
pH-Dependent Synthesis of Anisotropic Gold Nanostructures by Bioinspired Cysteine-Containing Peptides |
title_full |
pH-Dependent Synthesis of Anisotropic Gold Nanostructures by Bioinspired Cysteine-Containing Peptides |
title_fullStr |
pH-Dependent Synthesis of Anisotropic Gold Nanostructures by Bioinspired Cysteine-Containing Peptides |
title_full_unstemmed |
pH-Dependent Synthesis of Anisotropic Gold Nanostructures by Bioinspired Cysteine-Containing Peptides |
title_sort |
pH-Dependent Synthesis of Anisotropic Gold Nanostructures by Bioinspired Cysteine-Containing Peptides |
author |
Tofanello, Aryane |
author_facet |
Tofanello, Aryane Miranda, Erica G. A. Dias, Igor W. R. Lanfredi, Alexandre J. C. Arantes, Jeverson T. Juliano, Maria A. [UNIFESP] Nantes, Iseli L. |
author_role |
author |
author2 |
Miranda, Erica G. A. Dias, Igor W. R. Lanfredi, Alexandre J. C. Arantes, Jeverson T. Juliano, Maria A. [UNIFESP] Nantes, Iseli L. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Tofanello, Aryane Miranda, Erica G. A. Dias, Igor W. R. Lanfredi, Alexandre J. C. Arantes, Jeverson T. Juliano, Maria A. [UNIFESP] Nantes, Iseli L. |
description |
In the present study, alkaline peptides AAAXCX (X = lysine or arginine residues) were designed based on the conserved motif of the enzyme thioredoxin and used for the synthesis of gold nanoparticles (GNPs) in the pH range of 2-11. These peptides were compared with free cysteine, the counterpart acidic peptides AAAECE and gamma-ECG (glutathione), and the neutral peptide AAAACA. The objective was to investigate the effect of the amino acids neighboring a cysteine residue on the pH-dependent synthesis of gold nanocrystals. Kohn-Sham density functional theory (KS-DFT) calculations indicated an increase in the reducing capacity of AAAKCK favored by the successive deprotonation of their ionizable groups at increasing pH values. Experimentally, it was observed that gold speciation and the peptide structure also have a strong influence on the synthesis and stabilization of GNPs. AAAKCK produced GNPs at room temperature, in the whole investigated pH range. By contrast, alkaline pH was the best condition for the synthesis of GNP assisted by the AAARCR peptide. The acidic peptides produced GNPs only in the presence of polyethylene glycol, and the synthesis using AAAECE and gamma-ECG also required heating. The ionization state of AAAKCK had a strong influence on the preferential growth of the GNPs. Therefore, pH had a remarkable effect on the synthesis, kinetics, size, shape, and polydispersity of GNPs produced using AAAKCK. The AAAKCK peptide produced anisotropic decahedral and platelike nanocrystals at acidic pH values and spherical GNPs at alkaline pH values. Both alkaline peptides were also efficient capping agents for GNPs, but they produced a significant difference in the zeta potential, probably because of different orientations on the gold surface. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2019-07-22T15:46:43Z |
dc.date.available.fl_str_mv |
2019-07-22T15:46:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Acs Omega. Washington, v. 1, n. 3, p. 424-434, 2016. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/51039 http://dx.doi.org/10.1021/acsomega.6b00140 |
dc.identifier.issn.none.fl_str_mv |
2470-1343 |
dc.identifier.file.none.fl_str_mv |
WOS000391204000013.pdf |
dc.identifier.doi.none.fl_str_mv |
10.1021/acsomega.6b00140 |
dc.identifier.wos.none.fl_str_mv |
WOS:000391204000013 |
identifier_str_mv |
Acs Omega. Washington, v. 1, n. 3, p. 424-434, 2016. 2470-1343 WOS000391204000013.pdf 10.1021/acsomega.6b00140 WOS:000391204000013 |
url |
http://repositorio.unifesp.br/handle/11600/51039 http://dx.doi.org/10.1021/acsomega.6b00140 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
424-434 |
dc.publisher.none.fl_str_mv |
Amer Chemical Soc |
publisher.none.fl_str_mv |
Amer Chemical Soc |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
|
_version_ |
1802764140880592896 |