L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury

Detalhes bibliográficos
Autor(a) principal: Taha, Murched Omar [UNIFESP]
Data de Publicação: 2012
Outros Autores: Caricati-Neto, Afonso [UNIFESP], Miranda-Ferreira, Regiane [UNIFESP], Simões, Manuel de Jesus [UNIFESP], Monteiro, Hugo Pequeno [UNIFESP], Fagundes, Djalma José [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502012000900005
http://repositorio.unifesp.br/handle/11600/7306
Resumo: PURPOSE: To investigate the effects of intravenous L-arginine (LG) infusion on liver morphology, function and proinflammatory response of cytokines during the early phase of ischemia-reperfusion injury (IRI). METHODS: Thirty rabbits were subjected to 60 minutes of hepatic ischemia and 120 minutes of reperfusion. An intravenous injection of saline or L-arginine was administered five minutes before the ischemia and five minutes before initiating the reperfusion and at the 55th and 115th minutes after the ischemia. Samples were collected for histological analysis of the liver and measurements of the serum AST, ALT and LDH and the cytokines IL-6 and TNF-alpha. RESULTS: It was observed a significant reduction of sinusoidal congestion, cytoplasmic vacuolization, infiltration of polymorphonuclear leukocyte, nuclear pyknosis, necrosis and steatosis in liver tissue, as well as AST, ALT and LDH after injection of LG in the ischemia (p <0.001). Lower levels of IL-6 and TNF-alpha were associated with LG infusion during ischemia. Higher levels these proteins were observed in animals receiving LG during reperfusion. CONCLUSION: L-arginine protects the liver against ischemia/reperfusion injury, mainly when is administered during the ischemic phase.
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spelling L-arginine in the ischemic phase protects against liver ischemia-reperfusion injuryA L-arginina durante a fase isquêmica protege o fígado das lesões de isquemia e reperfusãoNitric OxideArginineWarm IschemiaCytokinesAntioxidantsInterleukin-6LiverRabittsÓxido NítricoArgininaIsquemia QuenteCitocinasAntioxidantesInterleucina-6FígadoCoelhosPURPOSE: To investigate the effects of intravenous L-arginine (LG) infusion on liver morphology, function and proinflammatory response of cytokines during the early phase of ischemia-reperfusion injury (IRI). METHODS: Thirty rabbits were subjected to 60 minutes of hepatic ischemia and 120 minutes of reperfusion. An intravenous injection of saline or L-arginine was administered five minutes before the ischemia and five minutes before initiating the reperfusion and at the 55th and 115th minutes after the ischemia. Samples were collected for histological analysis of the liver and measurements of the serum AST, ALT and LDH and the cytokines IL-6 and TNF-alpha. RESULTS: It was observed a significant reduction of sinusoidal congestion, cytoplasmic vacuolization, infiltration of polymorphonuclear leukocyte, nuclear pyknosis, necrosis and steatosis in liver tissue, as well as AST, ALT and LDH after injection of LG in the ischemia (p <0.001). Lower levels of IL-6 and TNF-alpha were associated with LG infusion during ischemia. Higher levels these proteins were observed in animals receiving LG during reperfusion. CONCLUSION: L-arginine protects the liver against ischemia/reperfusion injury, mainly when is administered during the ischemic phase.OBJETIVO: Investigar os efeitos da infusão endovenosa da L-arginina (LG) na morfologia, função e resposta de citocinas pró-inflamatórias do fígado durante a fase precoce da lesão de isquemia e reperfusão (IRI). MÉTODOS: Trinta coelhos foram submetidos a 60 minutos de isquemia hepática e 120 minutos de reperfusão. Foi administrada injecção intravenosa de solução salina ou L-arginina aos cinco minutos antes de iniciar a isquemia e cinco minutos antes de iniciar a reperfusão e aos 55 e 115 minutos após o início da isquemia. Realizou-se análise histológica do fígado e dosagens séricas de AST, ALT, LDH, citocinas IL-6 e TNF-alfa. RESULTADOS: Ocorreu redução significante da congestão sinusoidal, vacuolização citoplasmática, infiltração de leucócitos polimorfonucleares, picnose nuclear, necrose e esteatose no tecido hepático, assim como nos níveis de AST, ALT e LDH após a injeção da LG na isquemia (p<0,001). Níveis mais baixos de IL-6 e TNF-alfa foram associados com a infusão LG durante a isquemia. Níveis mais elevados dessas proteínas foram observados nos animais que receberam LG durante a reperfusão. CONCLUSÃO: A L-arginina protegeu o fígado contra a lesão de isquemia e reperfusão principalmente quando administrada durante a fase de isquemia.Universidade Federal de São Paulo (UNIFESP) Department of Surgery Operative Technique and Experimental Surgery DivisionUniversidade Federal de São Paulo (UNIFESP) Department of PharmacologyUniversidade Federal de São Paulo (UNIFESP) Department of Morphology Histology and Structural Biology DivisionUniversidade Federal de São Paulo (UNIFESP) Department of BiochemistryUNIFESP, Department of Surgery Operative Technique and Experimental Surgery DivisionUNIFESP, Department of PharmacologyUNIFESP, Department of Morphology Histology and Structural Biology DivisionUNIFESP, Department of BiochemistrySciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)Taha, Murched Omar [UNIFESP]Caricati-Neto, Afonso [UNIFESP]Miranda-Ferreira, Regiane [UNIFESP]Simões, Manuel de Jesus [UNIFESP]Monteiro, Hugo Pequeno [UNIFESP]Fagundes, Djalma José [UNIFESP]2015-06-14T13:44:57Z2015-06-14T13:44:57Z2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion616-623application/pdfhttp://dx.doi.org/10.1590/S0102-86502012000900005Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 27, n. 9, p. 616-623, 2012.10.1590/S0102-86502012000900005S0102-86502012000900005.pdf0102-8650S0102-86502012000900005http://repositorio.unifesp.br/handle/11600/7306WOS:000308133700005engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-03T14:35:52Zoai:repositorio.unifesp.br/:11600/7306Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-03T14:35:52Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury
A L-arginina durante a fase isquêmica protege o fígado das lesões de isquemia e reperfusão
title L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury
spellingShingle L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury
Taha, Murched Omar [UNIFESP]
Nitric Oxide
Arginine
Warm Ischemia
Cytokines
Antioxidants
Interleukin-6
Liver
Rabitts
Óxido Nítrico
Arginina
Isquemia Quente
Citocinas
Antioxidantes
Interleucina-6
Fígado
Coelhos
title_short L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury
title_full L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury
title_fullStr L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury
title_full_unstemmed L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury
title_sort L-arginine in the ischemic phase protects against liver ischemia-reperfusion injury
author Taha, Murched Omar [UNIFESP]
author_facet Taha, Murched Omar [UNIFESP]
Caricati-Neto, Afonso [UNIFESP]
Miranda-Ferreira, Regiane [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Monteiro, Hugo Pequeno [UNIFESP]
Fagundes, Djalma José [UNIFESP]
author_role author
author2 Caricati-Neto, Afonso [UNIFESP]
Miranda-Ferreira, Regiane [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Monteiro, Hugo Pequeno [UNIFESP]
Fagundes, Djalma José [UNIFESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Taha, Murched Omar [UNIFESP]
Caricati-Neto, Afonso [UNIFESP]
Miranda-Ferreira, Regiane [UNIFESP]
Simões, Manuel de Jesus [UNIFESP]
Monteiro, Hugo Pequeno [UNIFESP]
Fagundes, Djalma José [UNIFESP]
dc.subject.por.fl_str_mv Nitric Oxide
Arginine
Warm Ischemia
Cytokines
Antioxidants
Interleukin-6
Liver
Rabitts
Óxido Nítrico
Arginina
Isquemia Quente
Citocinas
Antioxidantes
Interleucina-6
Fígado
Coelhos
topic Nitric Oxide
Arginine
Warm Ischemia
Cytokines
Antioxidants
Interleukin-6
Liver
Rabitts
Óxido Nítrico
Arginina
Isquemia Quente
Citocinas
Antioxidantes
Interleucina-6
Fígado
Coelhos
description PURPOSE: To investigate the effects of intravenous L-arginine (LG) infusion on liver morphology, function and proinflammatory response of cytokines during the early phase of ischemia-reperfusion injury (IRI). METHODS: Thirty rabbits were subjected to 60 minutes of hepatic ischemia and 120 minutes of reperfusion. An intravenous injection of saline or L-arginine was administered five minutes before the ischemia and five minutes before initiating the reperfusion and at the 55th and 115th minutes after the ischemia. Samples were collected for histological analysis of the liver and measurements of the serum AST, ALT and LDH and the cytokines IL-6 and TNF-alpha. RESULTS: It was observed a significant reduction of sinusoidal congestion, cytoplasmic vacuolization, infiltration of polymorphonuclear leukocyte, nuclear pyknosis, necrosis and steatosis in liver tissue, as well as AST, ALT and LDH after injection of LG in the ischemia (p <0.001). Lower levels of IL-6 and TNF-alpha were associated with LG infusion during ischemia. Higher levels these proteins were observed in animals receiving LG during reperfusion. CONCLUSION: L-arginine protects the liver against ischemia/reperfusion injury, mainly when is administered during the ischemic phase.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-01
2015-06-14T13:44:57Z
2015-06-14T13:44:57Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502012000900005
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 27, n. 9, p. 616-623, 2012.
10.1590/S0102-86502012000900005
S0102-86502012000900005.pdf
0102-8650
S0102-86502012000900005
http://repositorio.unifesp.br/handle/11600/7306
WOS:000308133700005
url http://dx.doi.org/10.1590/S0102-86502012000900005
http://repositorio.unifesp.br/handle/11600/7306
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 27, n. 9, p. 616-623, 2012.
10.1590/S0102-86502012000900005
S0102-86502012000900005.pdf
0102-8650
S0102-86502012000900005
WOS:000308133700005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 616-623
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
_version_ 1814268294083903488

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