Central and peripheral effects of physical exercise without weight reduction in obese and lean mice
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | https://repositorio.unifesp.br/handle/11600/55624 http://dx.doi.org/10.1042/BSR20171033 |
Resumo: | To investigate the central (hypothalamic) and peripheral effects of exercise without body weight change in diet-induced obesity (DIO). Twelve-week-old male C57Bl/6 mice received a control (C) or a high-fat diet (H). Half of them had free access to running wheels for 5 days/week for 10 weeks (CE) and HE, respectively). Hypothalamic expression of genes related to energy homeostasis, and leptin (Stat3 and p-Stat3) and insulin (Akt and p-Akt) signaling were evaluated. Glucose and leptin tolerance, peripheral insulin sensitivity, and plasma insulin, leptin and adiponectin were determined. Perigonadal and retroperitoneal fat depots were increased by diet but reduced by exercise despite lack of effect of exercise on body weight. Blood glucose during intraperitoneal glucose tolerance test (ipGTT) was higher and glucose decay during intraperitoneal insulin tolerance test (ipITT) was lower in H and HE compared with C and CE. Exercise increased liver p-Akt expression and reduced fast glycemia. High-fat diet increased plasma insulin and leptin. Exercise had no effect on insulin but decreased leptin and increased adiponectin. Leptin inhibited food intake in all groups. Hypothalamic total and p-Stat3 and Akt were similar amongst the groups despite higher plasma levels of leptin and insulin in H and HE mice. High-fat diet modulated gene expression favoring a positive energy balance. Exercise only marginally changed the gene expression. Exercise induced positive changes (decreased fast glycemia and fat depots; increased liver insulin signaling and adiponectin concentration) without weight loss. Thus, despite reducing body weight could bring additional benefits, the effects of exercise must not be overlooked when weight reduction is not achieved. |
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Carvalho, Francine Pereira de [UNIFESP]Moretto, Thais Ludmilla [UNIFESP]Benfato, Izabelle Dias [UNIFESP]Barthichoto, Marcela [UNIFESP]Ferreira, Sandra MaraCosta-Junior, Jose MariaOliveira, Camila Aparecida Machado de [UNIFESP]2020-07-20T16:30:59Z2020-07-20T16:30:59Z2018Bioscience Reports. London, v. 38, p. -, 2018.0144-8463https://repositorio.unifesp.br/handle/11600/55624http://dx.doi.org/10.1042/BSR20171033WOS000431504200007.pdf10.1042/BSR20171033WOS:000431504200007To investigate the central (hypothalamic) and peripheral effects of exercise without body weight change in diet-induced obesity (DIO). Twelve-week-old male C57Bl/6 mice received a control (C) or a high-fat diet (H). Half of them had free access to running wheels for 5 days/week for 10 weeks (CE) and HE, respectively). Hypothalamic expression of genes related to energy homeostasis, and leptin (Stat3 and p-Stat3) and insulin (Akt and p-Akt) signaling were evaluated. Glucose and leptin tolerance, peripheral insulin sensitivity, and plasma insulin, leptin and adiponectin were determined. Perigonadal and retroperitoneal fat depots were increased by diet but reduced by exercise despite lack of effect of exercise on body weight. Blood glucose during intraperitoneal glucose tolerance test (ipGTT) was higher and glucose decay during intraperitoneal insulin tolerance test (ipITT) was lower in H and HE compared with C and CE. Exercise increased liver p-Akt expression and reduced fast glycemia. High-fat diet increased plasma insulin and leptin. Exercise had no effect on insulin but decreased leptin and increased adiponectin. Leptin inhibited food intake in all groups. Hypothalamic total and p-Stat3 and Akt were similar amongst the groups despite higher plasma levels of leptin and insulin in H and HE mice. High-fat diet modulated gene expression favoring a positive energy balance. Exercise only marginally changed the gene expression. Exercise induced positive changes (decreased fast glycemia and fat depots; increased liver insulin signaling and adiponectin concentration) without weight loss. Thus, despite reducing body weight could bring additional benefits, the effects of exercise must not be overlooked when weight reduction is not achieved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Sao Paulo, Programa Posgrad Interdisciplinar Ciencias Saude, Santos, SP, BrazilUniv Fed Sao Paulo, Programa Posgrad Alimentos Nutr & Saude, Santos, SP, BrazilUniv Estadual Campinas Unicamp, Inst Biol, Dept Biol Func & Estrutural, Campinas, SP, BrazilUniv Fed Sao Paulo, Inst Saude & Soc, Dept Biociencias, Santos, SP, BrazilUniv Fed Sao Paulo, Programa Posgrad Interdisciplinar Ciencias Saude, Santos, SP, BrazilUniv Fed Sao Paulo, Programa Posgrad Alimentos Nutr & Saude, Santos, SP, BrazilUniv Fed Sao Paulo, Inst Saude & Soc, Dept Biociencias, Santos, SP, BrazilFAPESP: 2011/05932-3FAPESP: 2013/01624-8Web of Science-engPortland Press LtdBioscience ReportsCentral and peripheral effects of physical exercise without weight reduction in obese and lean miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondonv. 38info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALWOS000431504200007.pdfapplication/pdf908585${dspace.ui.url}/bitstream/11600/55624/1/WOS000431504200007.pdf6a52e003d19092708cb22b5036382434MD51open accessTEXTWOS000431504200007.pdf.txtWOS000431504200007.pdf.txtExtracted texttext/plain56067${dspace.ui.url}/bitstream/11600/55624/5/WOS000431504200007.pdf.txt35fe180debf6d23b6039a3b879cf6226MD55open accessTHUMBNAILWOS000431504200007.pdf.jpgWOS000431504200007.pdf.jpgIM Thumbnailimage/jpeg7391${dspace.ui.url}/bitstream/11600/55624/7/WOS000431504200007.pdf.jpg4834419e675f4955e52a7ca1637983e0MD57open access11600/556242023-06-05 19:17:08.577open accessoai:repositorio.unifesp.br:11600/55624Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652023-06-05T22:17:08Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Central and peripheral effects of physical exercise without weight reduction in obese and lean mice |
title |
Central and peripheral effects of physical exercise without weight reduction in obese and lean mice |
spellingShingle |
Central and peripheral effects of physical exercise without weight reduction in obese and lean mice Carvalho, Francine Pereira de [UNIFESP] |
title_short |
Central and peripheral effects of physical exercise without weight reduction in obese and lean mice |
title_full |
Central and peripheral effects of physical exercise without weight reduction in obese and lean mice |
title_fullStr |
Central and peripheral effects of physical exercise without weight reduction in obese and lean mice |
title_full_unstemmed |
Central and peripheral effects of physical exercise without weight reduction in obese and lean mice |
title_sort |
Central and peripheral effects of physical exercise without weight reduction in obese and lean mice |
author |
Carvalho, Francine Pereira de [UNIFESP] |
author_facet |
Carvalho, Francine Pereira de [UNIFESP] Moretto, Thais Ludmilla [UNIFESP] Benfato, Izabelle Dias [UNIFESP] Barthichoto, Marcela [UNIFESP] Ferreira, Sandra Mara Costa-Junior, Jose Maria Oliveira, Camila Aparecida Machado de [UNIFESP] |
author_role |
author |
author2 |
Moretto, Thais Ludmilla [UNIFESP] Benfato, Izabelle Dias [UNIFESP] Barthichoto, Marcela [UNIFESP] Ferreira, Sandra Mara Costa-Junior, Jose Maria Oliveira, Camila Aparecida Machado de [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Carvalho, Francine Pereira de [UNIFESP] Moretto, Thais Ludmilla [UNIFESP] Benfato, Izabelle Dias [UNIFESP] Barthichoto, Marcela [UNIFESP] Ferreira, Sandra Mara Costa-Junior, Jose Maria Oliveira, Camila Aparecida Machado de [UNIFESP] |
description |
To investigate the central (hypothalamic) and peripheral effects of exercise without body weight change in diet-induced obesity (DIO). Twelve-week-old male C57Bl/6 mice received a control (C) or a high-fat diet (H). Half of them had free access to running wheels for 5 days/week for 10 weeks (CE) and HE, respectively). Hypothalamic expression of genes related to energy homeostasis, and leptin (Stat3 and p-Stat3) and insulin (Akt and p-Akt) signaling were evaluated. Glucose and leptin tolerance, peripheral insulin sensitivity, and plasma insulin, leptin and adiponectin were determined. Perigonadal and retroperitoneal fat depots were increased by diet but reduced by exercise despite lack of effect of exercise on body weight. Blood glucose during intraperitoneal glucose tolerance test (ipGTT) was higher and glucose decay during intraperitoneal insulin tolerance test (ipITT) was lower in H and HE compared with C and CE. Exercise increased liver p-Akt expression and reduced fast glycemia. High-fat diet increased plasma insulin and leptin. Exercise had no effect on insulin but decreased leptin and increased adiponectin. Leptin inhibited food intake in all groups. Hypothalamic total and p-Stat3 and Akt were similar amongst the groups despite higher plasma levels of leptin and insulin in H and HE mice. High-fat diet modulated gene expression favoring a positive energy balance. Exercise only marginally changed the gene expression. Exercise induced positive changes (decreased fast glycemia and fat depots; increased liver insulin signaling and adiponectin concentration) without weight loss. Thus, despite reducing body weight could bring additional benefits, the effects of exercise must not be overlooked when weight reduction is not achieved. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2020-07-20T16:30:59Z |
dc.date.available.fl_str_mv |
2020-07-20T16:30:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Bioscience Reports. London, v. 38, p. -, 2018. |
dc.identifier.uri.fl_str_mv |
https://repositorio.unifesp.br/handle/11600/55624 http://dx.doi.org/10.1042/BSR20171033 |
dc.identifier.issn.none.fl_str_mv |
0144-8463 |
dc.identifier.file.none.fl_str_mv |
WOS000431504200007.pdf |
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10.1042/BSR20171033 |
dc.identifier.wos.none.fl_str_mv |
WOS:000431504200007 |
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Bioscience Reports. London, v. 38, p. -, 2018. 0144-8463 WOS000431504200007.pdf 10.1042/BSR20171033 WOS:000431504200007 |
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https://repositorio.unifesp.br/handle/11600/55624 http://dx.doi.org/10.1042/BSR20171033 |
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Portland Press Ltd |
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