Characterization of a Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequence

Detalhes bibliográficos
Autor(a) principal: Cotrim, Paulo Cesar [UNIFESP]
Data de Publicação: 1989
Outros Autores: Cummings, Leda Maria [UNIFESP], Silveira, Jose Franco da [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0074-02761989000200001
http://repositorio.unifesp.br/handle/11600/242
Resumo: We have isolated a clone of Trypanosoma cruzi genimic DNA, lambda 3b2-5, which contains sequences that are reiterated in the genome. Northtern blot analysis showed that clone 3b2-5 hybridizes to 1,200-5,000 bases different mRNA species. The number of mRNAs species hybridized to clone 3b2-5 exceeds its coding capacity showing that this clone carries sequences that are common to several mRNAs species and conserved in the poly A(+) RNA. These sequences are not homologous to the T. cruzi spliced leader sequence, since clone 3b2-5 hybridize to a synthetic 20 nucleotice complementary to the spliced leader sequence. Clone 3b2-5 does not hybridize to DNA and RNA from several genera of Trypanosomatidae and other Trypanosoma species indicating that it carries T. cruzi species-specific sequences.
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spelling Characterization of a Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequenceCaracterização de um fragmento genômico de Trypanosoma cruzi complementar a diferentes espécies específicas de ARNs mensageiros mas diferente da seqüência líderTrypanosoma cruzigenimic clonesspecies-specific sequencesrepetitive DNAtranscriptional propertiesTrypanosoma cruziclones genômicossequências espécie-específicasADN repetitivopropriedade trasncricionaisWe have isolated a clone of Trypanosoma cruzi genimic DNA, lambda 3b2-5, which contains sequences that are reiterated in the genome. Northtern blot analysis showed that clone 3b2-5 hybridizes to 1,200-5,000 bases different mRNA species. The number of mRNAs species hybridized to clone 3b2-5 exceeds its coding capacity showing that this clone carries sequences that are common to several mRNAs species and conserved in the poly A(+) RNA. These sequences are not homologous to the T. cruzi spliced leader sequence, since clone 3b2-5 hybridize to a synthetic 20 nucleotice complementary to the spliced leader sequence. Clone 3b2-5 does not hybridize to DNA and RNA from several genera of Trypanosomatidae and other Trypanosoma species indicating that it carries T. cruzi species-specific sequences.Foi isolado um clone recombinante (lambda 3b2-5) de ADN genômico de Trypanosoma cruzi, o qual contém seqüências que estão reiteradas no genoma. Análise através da técnica de Northern blot mostrou que o clone 3b2-5 hibridiza com diferentes espécies de ARNs mensageiros de 1.200 a 5.000 bases. O número de espécies de ARNs mensageiros que hibridiza com o clone3b2-5 excede sua capacidade codante, sugerindo que este clone apresenta seqüências que são comuns a muitas espécies de ARNs mensageiros e conservadas no ARN poliadenilado. Estas seqüências não são homológas a seqüência líder presente nos ARNs de T. cruzi, pois, o clone 3b2-5 não hibridiza com um nucleotídeo sintético complementar a seqüência líder. O clone 3b2-5 não hibridiza com o ADN e ARN de diferentes gêneros de tripanosomatídeos e outras espécies de tripanosomas indicando que ele apresenta seqüências específicas para o T. cruzi.Escola Paulista de Medicina Departamento de Microbiologia, Imunologia e ParasitologiaUNIFESP, EPM, Depto. de Microbiologia, Imunologia e ParasitologiaSciELOInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Federal de São Paulo (UNIFESP)Cotrim, Paulo Cesar [UNIFESP]Cummings, Leda Maria [UNIFESP]Silveira, Jose Franco da [UNIFESP]2015-06-14T13:24:18Z2015-06-14T13:24:18Z1989-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion143-149application/pdfhttp://dx.doi.org/10.1590/S0074-02761989000200001Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 84, n. 2, p. 143-149, 1989.10.1590/S0074-02761989000200001S0074-02761989000200001.pdf0074-0276S0074-02761989000200001http://repositorio.unifesp.br/handle/11600/242WOS:A1989AK16200001engMemórias do Instituto Oswaldo Cruzinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T15:46:10Zoai:repositorio.unifesp.br/:11600/242Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T15:46:10Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Characterization of a Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequence
Caracterização de um fragmento genômico de Trypanosoma cruzi complementar a diferentes espécies específicas de ARNs mensageiros mas diferente da seqüência líder
title Characterization of a Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequence
spellingShingle Characterization of a Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequence
Cotrim, Paulo Cesar [UNIFESP]
Trypanosoma cruzi
genimic clones
species-specific sequences
repetitive DNA
transcriptional properties
Trypanosoma cruzi
clones genômicos
sequências espécie-específicas
ADN repetitivo
propriedade trasncricionais
title_short Characterization of a Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequence
title_full Characterization of a Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequence
title_fullStr Characterization of a Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequence
title_full_unstemmed Characterization of a Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequence
title_sort Characterization of a Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequence
author Cotrim, Paulo Cesar [UNIFESP]
author_facet Cotrim, Paulo Cesar [UNIFESP]
Cummings, Leda Maria [UNIFESP]
Silveira, Jose Franco da [UNIFESP]
author_role author
author2 Cummings, Leda Maria [UNIFESP]
Silveira, Jose Franco da [UNIFESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Cotrim, Paulo Cesar [UNIFESP]
Cummings, Leda Maria [UNIFESP]
Silveira, Jose Franco da [UNIFESP]
dc.subject.por.fl_str_mv Trypanosoma cruzi
genimic clones
species-specific sequences
repetitive DNA
transcriptional properties
Trypanosoma cruzi
clones genômicos
sequências espécie-específicas
ADN repetitivo
propriedade trasncricionais
topic Trypanosoma cruzi
genimic clones
species-specific sequences
repetitive DNA
transcriptional properties
Trypanosoma cruzi
clones genômicos
sequências espécie-específicas
ADN repetitivo
propriedade trasncricionais
description We have isolated a clone of Trypanosoma cruzi genimic DNA, lambda 3b2-5, which contains sequences that are reiterated in the genome. Northtern blot analysis showed that clone 3b2-5 hybridizes to 1,200-5,000 bases different mRNA species. The number of mRNAs species hybridized to clone 3b2-5 exceeds its coding capacity showing that this clone carries sequences that are common to several mRNAs species and conserved in the poly A(+) RNA. These sequences are not homologous to the T. cruzi spliced leader sequence, since clone 3b2-5 hybridize to a synthetic 20 nucleotice complementary to the spliced leader sequence. Clone 3b2-5 does not hybridize to DNA and RNA from several genera of Trypanosomatidae and other Trypanosoma species indicating that it carries T. cruzi species-specific sequences.
publishDate 1989
dc.date.none.fl_str_mv 1989-06-01
2015-06-14T13:24:18Z
2015-06-14T13:24:18Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0074-02761989000200001
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 84, n. 2, p. 143-149, 1989.
10.1590/S0074-02761989000200001
S0074-02761989000200001.pdf
0074-0276
S0074-02761989000200001
http://repositorio.unifesp.br/handle/11600/242
WOS:A1989AK16200001
url http://dx.doi.org/10.1590/S0074-02761989000200001
http://repositorio.unifesp.br/handle/11600/242
identifier_str_mv Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 84, n. 2, p. 143-149, 1989.
10.1590/S0074-02761989000200001
S0074-02761989000200001.pdf
0074-0276
S0074-02761989000200001
WOS:A1989AK16200001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 143-149
application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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