Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2002 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1046/j.1528-1157.43.s.5.26.x http://repositorio.unifesp.br/handle/11600/26681 |
Resumo: | Purpose: This study was performed to study the role of adenosine triphosphate (ATP) in the brain of pilocarpine-induced chronic epileptic rats.Methods: ATP-mediated changes in intracellular calcium were studied by the fura-2 method. lmmunohistochemistry and Western blotting methods were used to localize and quantify P2X7 receptors in these animals.Results: the fluorometric study in chronic rats revealed a biphasic response indicating the presence of P2X7 receptors. the Western blotting study showed an increase of 80% of P2X7 expression in chronic rats compared with the control group. P2X7 immunoreactivity resembled mossy fiber sprouting at the dentate gyrus of epileptic animals.Conclusions: These results suggest that purinergic receptors may participate in the pathophysiology of temporal lobe epilepsy. |
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3465 |
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Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studiesepilepsyATPP2X7pilocarpineratPurpose: This study was performed to study the role of adenosine triphosphate (ATP) in the brain of pilocarpine-induced chronic epileptic rats.Methods: ATP-mediated changes in intracellular calcium were studied by the fura-2 method. lmmunohistochemistry and Western blotting methods were used to localize and quantify P2X7 receptors in these animals.Results: the fluorometric study in chronic rats revealed a biphasic response indicating the presence of P2X7 receptors. the Western blotting study showed an increase of 80% of P2X7 expression in chronic rats compared with the control group. P2X7 immunoreactivity resembled mossy fiber sprouting at the dentate gyrus of epileptic animals.Conclusions: These results suggest that purinergic receptors may participate in the pathophysiology of temporal lobe epilepsy.Universidade Federal de São Paulo, Disciplina Neurol Expt, Dept Neurol Neurocirurg, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biofis, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Disciplina Neurol Expt, Dept Neurol Neurocirurg, BR-04023900 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Biofis, BR-04023900 São Paulo, BrazilWeb of ScienceBlackwell Publishing IncUniversidade Federal de São Paulo (UNIFESP)Vianna, Eduardo Paulo Morawski [UNIFESP]Ferreira, Alice Teixeira [UNIFESP]Naffah-Mazzacoratti, Maria da Graca [UNIFESP]Sanabria, Emilio Rafael Garrido [UNIFESP]Funke, M. [UNIFESP]Cavalheiro, Esper Abrão [UNIFESP]Fernandes, Maria Jose da Silva [UNIFESP]2016-01-24T12:33:09Z2016-01-24T12:33:09Z2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion227-229http://dx.doi.org/10.1046/j.1528-1157.43.s.5.26.xEpilepsia. Malden: Blackwell Publishing Inc, v. 43, p. 227-229, 2002.10.1046/j.1528-1157.43.s.5.26.x0013-9580http://repositorio.unifesp.br/handle/11600/26681WOS:000177445900042engEpilepsiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2022-07-08T10:51:09Zoai:repositorio.unifesp.br/:11600/26681Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652022-07-08T10:51:09Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies |
| title |
Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies |
| spellingShingle |
Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies Vianna, Eduardo Paulo Morawski [UNIFESP] epilepsy ATP P2X7 pilocarpine rat |
| title_short |
Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies |
| title_full |
Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies |
| title_fullStr |
Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies |
| title_full_unstemmed |
Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies |
| title_sort |
Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies |
| author |
Vianna, Eduardo Paulo Morawski [UNIFESP] |
| author_facet |
Vianna, Eduardo Paulo Morawski [UNIFESP] Ferreira, Alice Teixeira [UNIFESP] Naffah-Mazzacoratti, Maria da Graca [UNIFESP] Sanabria, Emilio Rafael Garrido [UNIFESP] Funke, M. [UNIFESP] Cavalheiro, Esper Abrão [UNIFESP] Fernandes, Maria Jose da Silva [UNIFESP] |
| author_role |
author |
| author2 |
Ferreira, Alice Teixeira [UNIFESP] Naffah-Mazzacoratti, Maria da Graca [UNIFESP] Sanabria, Emilio Rafael Garrido [UNIFESP] Funke, M. [UNIFESP] Cavalheiro, Esper Abrão [UNIFESP] Fernandes, Maria Jose da Silva [UNIFESP] |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
| dc.contributor.author.fl_str_mv |
Vianna, Eduardo Paulo Morawski [UNIFESP] Ferreira, Alice Teixeira [UNIFESP] Naffah-Mazzacoratti, Maria da Graca [UNIFESP] Sanabria, Emilio Rafael Garrido [UNIFESP] Funke, M. [UNIFESP] Cavalheiro, Esper Abrão [UNIFESP] Fernandes, Maria Jose da Silva [UNIFESP] |
| dc.subject.por.fl_str_mv |
epilepsy ATP P2X7 pilocarpine rat |
| topic |
epilepsy ATP P2X7 pilocarpine rat |
| description |
Purpose: This study was performed to study the role of adenosine triphosphate (ATP) in the brain of pilocarpine-induced chronic epileptic rats.Methods: ATP-mediated changes in intracellular calcium were studied by the fura-2 method. lmmunohistochemistry and Western blotting methods were used to localize and quantify P2X7 receptors in these animals.Results: the fluorometric study in chronic rats revealed a biphasic response indicating the presence of P2X7 receptors. the Western blotting study showed an increase of 80% of P2X7 expression in chronic rats compared with the control group. P2X7 immunoreactivity resembled mossy fiber sprouting at the dentate gyrus of epileptic animals.Conclusions: These results suggest that purinergic receptors may participate in the pathophysiology of temporal lobe epilepsy. |
| publishDate |
2002 |
| dc.date.none.fl_str_mv |
2002-01-01 2016-01-24T12:33:09Z 2016-01-24T12:33:09Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1046/j.1528-1157.43.s.5.26.x Epilepsia. Malden: Blackwell Publishing Inc, v. 43, p. 227-229, 2002. 10.1046/j.1528-1157.43.s.5.26.x 0013-9580 http://repositorio.unifesp.br/handle/11600/26681 WOS:000177445900042 |
| url |
http://dx.doi.org/10.1046/j.1528-1157.43.s.5.26.x http://repositorio.unifesp.br/handle/11600/26681 |
| identifier_str_mv |
Epilepsia. Malden: Blackwell Publishing Inc, v. 43, p. 227-229, 2002. 10.1046/j.1528-1157.43.s.5.26.x 0013-9580 WOS:000177445900042 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Epilepsia |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
227-229 |
| dc.publisher.none.fl_str_mv |
Blackwell Publishing Inc |
| publisher.none.fl_str_mv |
Blackwell Publishing Inc |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
| instname_str |
Universidade Federal de São Paulo (UNIFESP) |
| instacron_str |
UNIFESP |
| institution |
UNIFESP |
| reponame_str |
Repositório Institucional da UNIFESP |
| collection |
Repositório Institucional da UNIFESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
| repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
| _version_ |
1814268298317004800 |