Hemodynamic, morphometric and autonomic patterns in hypertensive rats - renin-angiotensin system modulation
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://repositorio.unifesp.br/handle/11600/5578 http://dx.doi.org/10.1590/S1807-59322010000100013 |
Resumo: | BACKGROUND: Spontaneously hypertensive rats develop left ventricular hypertrophy, increased blood pressure and blood pressure variability, which are important determinants of heart damage, like the activation of renin-angiotensin system. AIMS: To investigate the effects of the time-course of hypertension over 1) hemodynamic and autonomic patterns (blood pressure; blood pressure variability; heart rate); 2) left ventricular hypertrophy; and 3) local and systemic Renin-angiotensin system of the spontaneously hypertensive rats. METHODS: Male spontaneously hypertensive rats were randomized into two groups: young (n=13) and adult (n=12). Hemodynamic signals (blood pressure, heart rate), blood pressure variability (BPV) and spectral analysis of the autonomic components of blood pressure were analyzed. LEFT ventricular hypertrophy was measured by the ratio of LV mass to body weight (mg/g), by myocyte diameter (μm) and by relative fibrosis area (RFA, %). ACE and ACE2 activities were measured by fluorometry (UF/min), and plasma renin activity (PRA) was assessed by a radioimmunoassay (ng/mL/h). Cardiac gene expressions of Agt, Ace and Ace2 were quantified by RT-PCR (AU). RESULTS: The time-course of hypertension in spontaneously hypertensive rats increased BPV and reduced the alpha index in adult spontaneously hypertensive rats. Adult rats showed increases in left ventricular hypertrophy and in RFA. Compared to young spontaneously hypertensive rats, adult spontaneously hypertensive rats had lower cardiac ACE and ACE2 activities, and high levels of PRA. No change was observed in gene expression of Renin-angiotensin system components. CONCLUSIONS: The observed autonomic dysfunction and modulation of Renin-angiotensin system activity are contributing factors to end-organ damage in hypertension and could be interacting. Our findings suggest that the management of hypertensive disease must start before blood pressure reaches the highest stable levels and the consequent established end-organ damage is reached. |
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Zamo, Fernanda de Souza [UNIFESP]Lacchini, SilviaMostarda, Cristiano [UNIFESP]Chiavegatto, SilvanaMoraes-Silva, Ivana Cinthya [UNIFESP]Oliveira, Edilamar Menezes deIrigoyen, Maria Claudia [UNIFESP]Universidade Federal de São Paulo (UNIFESP)Universidade de São Paulo (USP)2015-06-14T13:41:31Z2015-06-14T13:41:31Z2010-01-01Clinics. Faculdade de Medicina / USP, v. 65, n. 1, p. 85-92, 2010.1807-5932http://repositorio.unifesp.br/handle/11600/5578http://dx.doi.org/10.1590/S1807-59322010000100013S1807-59322010000100013.pdfS1807-5932201000010001310.1590/S1807-59322010000100013WOS:000274628800012BACKGROUND: Spontaneously hypertensive rats develop left ventricular hypertrophy, increased blood pressure and blood pressure variability, which are important determinants of heart damage, like the activation of renin-angiotensin system. AIMS: To investigate the effects of the time-course of hypertension over 1) hemodynamic and autonomic patterns (blood pressure; blood pressure variability; heart rate); 2) left ventricular hypertrophy; and 3) local and systemic Renin-angiotensin system of the spontaneously hypertensive rats. METHODS: Male spontaneously hypertensive rats were randomized into two groups: young (n=13) and adult (n=12). Hemodynamic signals (blood pressure, heart rate), blood pressure variability (BPV) and spectral analysis of the autonomic components of blood pressure were analyzed. LEFT ventricular hypertrophy was measured by the ratio of LV mass to body weight (mg/g), by myocyte diameter (μm) and by relative fibrosis area (RFA, %). ACE and ACE2 activities were measured by fluorometry (UF/min), and plasma renin activity (PRA) was assessed by a radioimmunoassay (ng/mL/h). Cardiac gene expressions of Agt, Ace and Ace2 were quantified by RT-PCR (AU). RESULTS: The time-course of hypertension in spontaneously hypertensive rats increased BPV and reduced the alpha index in adult spontaneously hypertensive rats. Adult rats showed increases in left ventricular hypertrophy and in RFA. Compared to young spontaneously hypertensive rats, adult spontaneously hypertensive rats had lower cardiac ACE and ACE2 activities, and high levels of PRA. No change was observed in gene expression of Renin-angiotensin system components. CONCLUSIONS: The observed autonomic dysfunction and modulation of Renin-angiotensin system activity are contributing factors to end-organ damage in hypertension and could be interacting. Our findings suggest that the management of hypertensive disease must start before blood pressure reaches the highest stable levels and the consequent established end-organ damage is reached.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Federal University of São Paulo Nephrology DepartmentUniversidade de São Paulo Faculdade de Medicina Heart Institute (InCor)Universidade de São Paulo Institute of Biomedical Sciences Department of AnatomyUniversidade de São Paulo Institute of Biomedical Sciences Department of PharmacologyUNIFESP, Nephrology DepartmentFAPESP: 01/01129-0FAPESP: 01/00009-0SciELO85-92engFaculdade de Medicina / USPClinicsSHRHypertensionBlood pressure variabilityRASLVHHemodynamic, morphometric and autonomic patterns in hypertensive rats - renin-angiotensin system modulationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESPORIGINALS1807-59322010000100013.pdfapplication/pdf340551${dspace.ui.url}/bitstream/11600/5578/1/S1807-59322010000100013.pdf17e36698033ec1d59af4c3a82506c53cMD51open accessTEXTS1807-59322010000100013.pdf.txtS1807-59322010000100013.pdf.txtExtracted texttext/plain37562${dspace.ui.url}/bitstream/11600/5578/2/S1807-59322010000100013.pdf.txtf826f3f3898ea102f3d9962d4583e858MD52open access11600/55782021-10-05 21:53:32.498open accessoai:repositorio.unifesp.br:11600/5578Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestopendoar:34652021-10-06T00:53:32Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.en.fl_str_mv |
Hemodynamic, morphometric and autonomic patterns in hypertensive rats - renin-angiotensin system modulation |
title |
Hemodynamic, morphometric and autonomic patterns in hypertensive rats - renin-angiotensin system modulation |
spellingShingle |
Hemodynamic, morphometric and autonomic patterns in hypertensive rats - renin-angiotensin system modulation Zamo, Fernanda de Souza [UNIFESP] SHR Hypertension Blood pressure variability RAS LVH |
title_short |
Hemodynamic, morphometric and autonomic patterns in hypertensive rats - renin-angiotensin system modulation |
title_full |
Hemodynamic, morphometric and autonomic patterns in hypertensive rats - renin-angiotensin system modulation |
title_fullStr |
Hemodynamic, morphometric and autonomic patterns in hypertensive rats - renin-angiotensin system modulation |
title_full_unstemmed |
Hemodynamic, morphometric and autonomic patterns in hypertensive rats - renin-angiotensin system modulation |
title_sort |
Hemodynamic, morphometric and autonomic patterns in hypertensive rats - renin-angiotensin system modulation |
author |
Zamo, Fernanda de Souza [UNIFESP] |
author_facet |
Zamo, Fernanda de Souza [UNIFESP] Lacchini, Silvia Mostarda, Cristiano [UNIFESP] Chiavegatto, Silvana Moraes-Silva, Ivana Cinthya [UNIFESP] Oliveira, Edilamar Menezes de Irigoyen, Maria Claudia [UNIFESP] |
author_role |
author |
author2 |
Lacchini, Silvia Mostarda, Cristiano [UNIFESP] Chiavegatto, Silvana Moraes-Silva, Ivana Cinthya [UNIFESP] Oliveira, Edilamar Menezes de Irigoyen, Maria Claudia [UNIFESP] |
author2_role |
author author author author author author |
dc.contributor.institution.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Zamo, Fernanda de Souza [UNIFESP] Lacchini, Silvia Mostarda, Cristiano [UNIFESP] Chiavegatto, Silvana Moraes-Silva, Ivana Cinthya [UNIFESP] Oliveira, Edilamar Menezes de Irigoyen, Maria Claudia [UNIFESP] |
dc.subject.eng.fl_str_mv |
SHR Hypertension Blood pressure variability RAS LVH |
topic |
SHR Hypertension Blood pressure variability RAS LVH |
description |
BACKGROUND: Spontaneously hypertensive rats develop left ventricular hypertrophy, increased blood pressure and blood pressure variability, which are important determinants of heart damage, like the activation of renin-angiotensin system. AIMS: To investigate the effects of the time-course of hypertension over 1) hemodynamic and autonomic patterns (blood pressure; blood pressure variability; heart rate); 2) left ventricular hypertrophy; and 3) local and systemic Renin-angiotensin system of the spontaneously hypertensive rats. METHODS: Male spontaneously hypertensive rats were randomized into two groups: young (n=13) and adult (n=12). Hemodynamic signals (blood pressure, heart rate), blood pressure variability (BPV) and spectral analysis of the autonomic components of blood pressure were analyzed. LEFT ventricular hypertrophy was measured by the ratio of LV mass to body weight (mg/g), by myocyte diameter (μm) and by relative fibrosis area (RFA, %). ACE and ACE2 activities were measured by fluorometry (UF/min), and plasma renin activity (PRA) was assessed by a radioimmunoassay (ng/mL/h). Cardiac gene expressions of Agt, Ace and Ace2 were quantified by RT-PCR (AU). RESULTS: The time-course of hypertension in spontaneously hypertensive rats increased BPV and reduced the alpha index in adult spontaneously hypertensive rats. Adult rats showed increases in left ventricular hypertrophy and in RFA. Compared to young spontaneously hypertensive rats, adult spontaneously hypertensive rats had lower cardiac ACE and ACE2 activities, and high levels of PRA. No change was observed in gene expression of Renin-angiotensin system components. CONCLUSIONS: The observed autonomic dysfunction and modulation of Renin-angiotensin system activity are contributing factors to end-organ damage in hypertension and could be interacting. Our findings suggest that the management of hypertensive disease must start before blood pressure reaches the highest stable levels and the consequent established end-organ damage is reached. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-01-01 |
dc.date.accessioned.fl_str_mv |
2015-06-14T13:41:31Z |
dc.date.available.fl_str_mv |
2015-06-14T13:41:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
Clinics. Faculdade de Medicina / USP, v. 65, n. 1, p. 85-92, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.unifesp.br/handle/11600/5578 http://dx.doi.org/10.1590/S1807-59322010000100013 |
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1807-5932 |
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S1807-59322010000100013.pdf |
dc.identifier.scielo.none.fl_str_mv |
S1807-59322010000100013 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1807-59322010000100013 |
dc.identifier.wos.none.fl_str_mv |
WOS:000274628800012 |
identifier_str_mv |
Clinics. Faculdade de Medicina / USP, v. 65, n. 1, p. 85-92, 2010. 1807-5932 S1807-59322010000100013.pdf S1807-59322010000100013 10.1590/S1807-59322010000100013 WOS:000274628800012 |
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http://repositorio.unifesp.br/handle/11600/5578 http://dx.doi.org/10.1590/S1807-59322010000100013 |
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eng |
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85-92 |
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Faculdade de Medicina / USP |
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Faculdade de Medicina / USP |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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